Abstract:
:A striking and unexpected biochemical phenotype was found in an insertion mutant line in the model plant Arabidopsis thaliana. One of two investigated insertion mutant lines in the gene encoding the phosphate transporter PHT4;1 demonstrated a prominent loss of trienoic fatty acids, whereas the other insertion line was indistinguishable from wild type in this aspect. We demonstrate that the loss of trienoic fatty acids was due to a remnant inactive negative selection marker gene in this particular transposon tagged line, pht4;1-3. This constitutes a cautionary tale that warns of the importance to confirm the loss of this type of selection markers and the importance of verifying the relationship between a phenotype and genotype by more than one independent mutant line or alternatively genetic complementation.
journal_name
PeerJjournal_title
PeerJauthors
Nilsson AK,Andersson MXdoi
10.7717/peerj.4134subject
Has Abstractpub_date
2017-12-01 00:00:00pages
e4134issn
2167-8359pii
4134journal_volume
5pub_type
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