当前位置： SCI文献检索 > Scientific Reports期刊下所有文献 > Direct conversion of human fibroblasts into hepatocyte-like cells by ATF5, PROX1, FOXA2, FOXA3, and HNF4A transduction.

Direct conversion of human fibroblasts into hepatocyte-like cells by ATF5, PROX1, FOXA2, FOXA3, and HNF4A transduction.

Abstract：

:Recently, it has been reported that human hepatocyte-like cells can be generated from fibroblasts by direct reprogramming technology. However, the conversion efficiency of human induced hepatocyte-like cells (hiHeps) is not high enough. In addition, comparative analysis with the existing models of hepatocytes, such as human iPS cell-derived hepatocyte-like cells and primary human hepatocytes, has not been sufficiently carried out. In this study, we screened hepatic transcription factors for efficient direct hepatic reprogramming and compared hepatic functions between hiHeps and other existing hepatocyte models. We found that human fibroblasts were efficiently converted into hiHeps by using a combination of ATF5, PROX1, FOXA2, FOXA3, and HNF4A (albumin+/alpha-1 antitrypsin+ cells = 27%, asialoglycoprotein receptor 1+ cells = 22%). The CYP expression levels and CYP activities in hiHeps were higher than those in human iPS cell-derived hepatocyte-like cells, but lower than those in short-term (4 hr) cultured primary human hepatocytes and primary human hepatocytes collected immediately after thawing. These results suggested that functional hiHeps could be efficiently generated by ATF5, PROX1, FOXA2, FOXA3, and HNF4A transduction. We believe that hiHeps generated by our method will be useful for the drug-discovery activities such as hepatotoxicity screening and drug metabolism tests.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Nakamori D,Akamine H,Takayama K,Sakurai F,Mizuguchi H

doi

10.1038/s41598-017-16856-7

subject

Has Abstract

pub_date

2017-11-30 00:00:00

pages

16675

issue

1

issn

2045-2322

pii

10.1038/s41598-017-16856-7

journal_volume

7

pub_type

杂志文章

相关文献

Scientific Reports文献大全
  • Structural dynamics of Populus euphratica forests in different stages in the upper reaches of the Tarim River in China.

    abstract：:We selected four Populus euphratica Oliv. forest plots (100 m × 100 m) in the upper reaches of the Tarim River in the Xinjiang Uygur Autonomous Region of China. Each of the four forest plots was chosen to represent a different growth and death stage of P. euphratica forest: juvenile forest, mature forest, dying forest...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-60139-7

    authors： Miao N,Jiao P,Tao W,Li M,Li Z,Hu B,Moermond TC

    更新日期：2020-02-21 00:00:00

  • Efficacy and safety of multimodal analgesic techniques for preventing chronic postsurgery pain under different surgical categories: a meta-analysis.

    abstract：:The purpose of this meta-analysis was to compare the efficacy and safety of regional anesthesia to manage chronic postsurgery pain. A systematic search of PubMed, EmBase, and the Cochrane Central Register of Controlled Trials was performed to identify randomized controlled trials that focused on chronic pain frequency...

    journal_title：Scientific reports

    pub_type： 杂志文章,meta分析

    doi：10.1038/s41598-017-00813-5

    authors： Zhou J,Fan Y,Zhong J,Wen X,Chen H

    更新日期：2017-04-06 00:00:00

  • Effects of sediment smothering on the sponge holobiont with implications for dredging management.

    abstract：:One of the ways dredging can affect benthic habitats is through high levels of sediment deposition, which has the potential to smother sessile organisms such as sponges. In order to provide pressure-response values to sedimentation and tease apart the different cause-effect pathways of high turbidity, 5 sponge species...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-05243-x

    authors： Pineda MC,Strehlow B,Sternel M,Duckworth A,Haan JD,Jones R,Webster NS

    更新日期：2017-07-14 00:00:00

  • Transcriptome and long noncoding RNA sequencing of three extracellular vesicle subtypes released from the human colon cancer LIM1863 cell line.

    abstract：:Previously we reported that LIM1863 colorectal cancer (CRC) cells secrete three distinct extracellular vesicle subtypes - two subpopulations of exosomes (apical EpCAM-Exos and basolateral A33-Exos) and shed microvesicles (sMVs) - with distinct protein and miRNA signatures. Here, we extend our omics approach to underst...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep38397

    authors： Chen M,Xu R,Ji H,Greening DW,Rai A,Izumikawa K,Ishikawa H,Takahashi N,Simpson RJ

    更新日期：2016-12-05 00:00:00

  • Cellular Depletion of BRD8 Causes p53-Dependent Apoptosis and Induces a DNA Damage Response in Non-Stressed Cells.

    abstract：:Regulation of the chromatin state is crucial for biological processes such as the regulation of transcription, DNA replication, and DNA damage repair. Here we show that knockdown of the BRD8 bromodomain protein - a subunit of the p400/Tip60 complex - leads to p21 induction, and concomitant cell cycle arrest in G1/S. W...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-32323-3

    authors： Lashgari A,Fauteux M,Maréchal A,Gaudreau L

    更新日期：2018-09-20 00:00:00

  • EnSVMB: Metagenomics Fragments Classification using Ensemble SVM and BLAST.

    abstract：:Metagenomics brings in new discoveries and insights into the uncultured microbial world. One fundamental task in metagenomics analysis is to determine the taxonomy of raw sequence fragments. Modern sequencing technologies produce relatively short fragments and greatly increase the number of fragments, and thus make th...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-09947-y

    authors： Jiang Y,Wang J,Xia D,Yu G

    更新日期：2017-08-25 00:00:00

  • Towards a Physarum learning chip.

    abstract：:Networks of protoplasmic tubes of organism Physarum polycehpalum are macro-scale structures which optimally span multiple food sources to avoid repellents yet maximize coverage of attractants. When data are presented by configurations of attractants and behaviour of the slime mould is tuned by a range of repellents, t...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep19948

    authors： Whiting JG,Jones J,Bull L,Levin M,Adamatzky A

    更新日期：2016-02-03 00:00:00

  • Evaluation of relationships between onychomycosis and vascular diseases using sequential pattern mining.

    abstract：:Onychomycosis (OM) is a common nail disease. Although controversial, vascular diseases are considered independent predictors of OM and vice versa. Sequential pattern mining (SPM) has not been previously used for statistical analysis in dermatology, but it is an efficient method for identifying frequent association rul...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-35909-z

    authors： Bang CH,Yoon JW,Lee HJ,Lee JY,Park YM,Lee SJ,Lee JH

    更新日期：2018-12-14 00:00:00

  • Two-dimensional β-MnO₂ nanowire network with enhanced electrochemical capacitance.

    abstract：:Conventional crystalline β-MnO2 usually exhibits poor electrochemical activities due to the narrow tunnels in its rutile-type structure. In this study, we synthesized a novel 2D β-MnO2 network with long-range order assembled by β-MnO2 nanowires and demonstrated that the novel 2D β-MnO2 network exhibits enhanced electr...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep02193

    authors： Wei C,Pang H,Zhang B,Lu Q,Liang S,Gao F

    更新日期：2013-01-01 00:00:00

  • Particle size effect on sorting with optical lattice.

    abstract：:Transport of mesoscale particles due to driving flow fields or external forces on a periodic surface appears in many areas. Geometrical and physical characteristics of particles affect the velocities of the particles in these periodic landscapes. In this paper, we present a numerical simulation based on solving the La...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-75187-2

    authors： Madadi E,Biagooi M,Mohammadjafari F,Nedaaee Oskoee S

    更新日期：2020-10-26 00:00:00

  • Transcriptomic profile of Pea3 family members reveal regulatory codes for axon outgrowth and neuronal connection specificity.

    abstract：:PEA3 transcription factor subfamily is present in a variety of tissues with branching morphogenesis, and play a particularly significant role in neural circuit formation and specificity. Many target genes in axon guidance and cell-cell adhesion pathways have been identified for Pea3 transcription factor (but not for E...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-75089-3

    authors： Kandemir B,Gulfidan G,Arga KY,Yilmaz B,Kurnaz IA

    更新日期：2020-10-23 00:00:00

  • A Neural Network Approach to Identify the Peritumoral Invasive Areas in Glioblastoma Patients by Using MR Radiomics.

    abstract：:The challenge in the treatment of glioblastoma is the failure to identify the cancer invasive area outside the contrast-enhancing tumour which leads to the high local progression rate. Our study aims to identify its progression from the preoperative MR radiomics. 57 newly diagnosed cerebral glioblastoma patients were ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-66691-6

    authors： Yan JL,Li C,van der Hoorn A,Boonzaier NR,Matys T,Price SJ

    更新日期：2020-06-16 00:00:00

  • Laser- synthesized TiN nanoparticles as promising plasmonic alternative for biomedical applications.

    abstract：:Exhibiting a red-shifted absorption/scattering feature compared to conventional plasmonic metals, titanium nitride nanoparticles (TiN NPs) look as very promising candidates for biomedical applications, but these applications are still underexplored despite the presence of extensive data for conventional plasmonic coun...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-37519-1

    authors： Popov AA,Tselikov G,Dumas N,Berard C,Metwally K,Jones N,Al-Kattan A,Larrat B,Braguer D,Mensah S,Da Silva A,Estève MA,Kabashin AV

    更新日期：2019-02-04 00:00:00

  • Deficiency of the RIβ subunit of protein kinase A causes body tremor and impaired fear conditioning memory in rats.

    abstract：:The RIβ subunit of cAMP-dependent protein kinase (PKA), encoded by Prkar1b, is a neuronal isoform of the type I regulatory subunit of PKA. Mice lacking the RIβ subunit exhibit normal long-term potentiation (LTP) in the Schaffer collateral pathway of the hippocampus and normal behavior in the open-field and fear condit...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-021-81515-x

    authors： Hoang Trung H,Yoshihara T,Nakao A,Hayashida K,Hirata Y,Shirasuna K,Kuwamura M,Nakagawa Y,Kaneko T,Mori Y,Asano M,Kuramoto T

    更新日期：2021-01-21 00:00:00

  • Bioinformatical parsing of folding-on-binding proteins reveals their compositional and evolutionary sequence design.

    abstract：:Intrinsic disorder occurs when (part of) a protein remains unfolded during normal functioning. Intrinsically-disordered regions can contain segments that 'fold on binding' to another molecule. Here, we perform bioinformatical parsing of human 'folding-on-binding' (FB) proteins, into four subsets: Ordered regions, FB r...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep18586

    authors： Narasumani M,Harrison PM

    更新日期：2015-12-18 00:00:00

  • The Pressure induced by salt crystallization in confinement.

    abstract：:Salt crystallization is a major cause of weathering of rocks, artworks and monuments. Damage can only occur if crystals continue to grow in confinement, i.e. within the pore space of these materials, thus generating mechanical stress. We report the direct measurement, at the microscale, of the force exerted by growing...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep30856

    authors： Desarnaud J,Bonn D,Shahidzadeh N

    更新日期：2016-08-05 00:00:00

  • An unexpected genetic diversity pattern and a complex demographic history of a rare medicinal herb, Chinese asparagus (Asparagus cochinchinensis) in Korea.

    abstract：:Range-wide population studies of wide spread species are often associated with complex diversity patterns resulting from genetically divergent evolutionary significant units (ESUs). The compound evolutionary history creating such a pattern of diversity can be inferred through molecular analyses. Asparagus cochinchinen...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-46275-9

    authors： Lee SR,Park HS,Kim BY,Lee JH,Fan Q,Gaskin JF,Kim YD

    更新日期：2019-07-05 00:00:00

  • Direct observation of Thermomyces lanuginosus lipase diffusional states by Single Particle Tracking and their remodeling by mutations and inhibition.

    abstract：:Lipases are interfacially activated enzymes that catalyze the hydrolysis of ester bonds and constitute prime candidates for industrial and biotechnological applications ranging from detergent industry, to chiral organic synthesis. As a result, there is an incentive to understand the mechanisms underlying lipase activi...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-52539-1

    authors： Bohr SS,Lund PM,Kallenbach AS,Pinholt H,Thomsen J,Iversen L,Svendsen A,Christensen SM,Hatzakis NS

    更新日期：2019-11-07 00:00:00

  • Establishing next-generation pest control services in rice fields: eco-agriculture.

    abstract：:Pesticides are commonly used in food crop production systems to control crop pests and diseases and ensure maximum yield with high market value. However, the accumulation of these chemical inputs in crop fields increases risks to biodiversity and human health. In addition, people are increasingly seeking foods in whic...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-46688-6

    authors： Ali MP,Bari MN,Haque SS,Kabir MMM,Afrin S,Nowrin F,Islam MS,Landis DA

    更新日期：2019-07-15 00:00:00

  • Hepatic lipocalin 2 promotes liver fibrosis and portal hypertension.

    abstract：:Advanced fibrosis and portal hypertension influence short-term mortality. Lipocalin 2 (LCN2) regulates infection response and increases in liver injury. We explored the role of intrahepatic LCN2 in human alcoholic hepatitis (AH) with advanced fibrosis and portal hypertension and in experimental mouse fibrosis. We foun...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-72172-7

    authors： Chen J,Argemi J,Odena G,Xu MJ,Cai Y,Massey V,Parrish A,Vadigepalli R,Altamirano J,Cabezas J,Gines P,Caballeria J,Snider N,Sancho-Bru P,Akira S,Rusyn I,Gao B,Bataller R

    更新日期：2020-09-23 00:00:00

  • Reduction of hepatic fibrosis by overexpression of von Hippel-Lindau protein in experimental models of chronic liver disease.

    abstract：:Hypoxia-inducible factor (HIF)-1α and HIF-2α play an important role in liver fibrosis. von Hippel-Lindau protein (VHL), a key mediator of HIF-α, regulates fibrosis in an organ- and cell-specific way. In this study, human liver samples were collected from hepatitis C-, alcoholic-, and cholestatic-associated fibrotic an...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep41038

    authors： Wang J,Lu Z,Xu Z,Tian P,Miao H,Pan S,Song R,Sun X,Zhao B,Wang D,Ma Y,Song X,Zhang S,Liu L,Jiang H

    更新日期：2017-01-23 00:00:00

  • EGFP-EGF1-conjugated poly (lactic-co-glycolic acid) nanoparticles as a carrier for the delivery of CCR2- shRNA to atherosclerotic macrophage in vitro.

    abstract：:Reducing macrophage recruitment by silencing chemokine (C-C motif) receptor 2 (CCR2) expression is a promising therapeutic approach against atherosclerosis. However the transfection of macrophages with siRNA is often technically challenging. EGFP-EGF1-conjugated poly (lactic-co-glycolic acid) (PLGA) nanoparticles (ENP...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-76416-4

    authors： Wu Z,Chen C,Luo J,Davis JRJ,Zhang B,Tang L,Shi W,Liao D

    更新日期：2020-11-12 00:00:00

  • Exploration of phase structure evolution induced by alloying elements in Ti alloys via a chemical-short-range-order cluster model.

    abstract：:The prominent comprehensive properties of solid-solution- and intermetallic-based Ti alloys are derived from their diverse microstructures induced by multi-component alloying, which results in a chemical composition complexity. A cluster-plus-glue-atom model, characterizing the chemical short-range orders, was introdu...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-40302-5

    authors： Jiang B,Wang Q,Dong C,Liaw PK

    更新日期：2019-03-04 00:00:00

  • A Joint Modeling Approach for Childhood Meat, Fish and Egg Consumption and the Risk of Advanced Islet Autoimmunity.

    abstract：:Several dietary factors have been suspected to play a role in the development of advanced islet autoimmunity (IA) and/or type 1 diabetes (T1D), but the evidence is fragmentary. A prospective population-based cohort of 6081 Finnish newborn infants with HLA-DQB1-conferred susceptibility to T1D was followed up to 15 year...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-44196-1

    authors： Syrjälä E,Nevalainen J,Peltonen J,Takkinen HM,Hakola L,Åkerlund M,Veijola R,Ilonen J,Toppari J,Knip M,Virtanen SM

    更新日期：2019-05-23 00:00:00

  • Wave Manipulations by Coherent Perfect Channeling.

    abstract：:We show that through the wave energy conserved and reversible process of coherent interactions of scalar waves in a multi-channel system joint by a common junction, it is possible to generate outgoing waves only in certain channels by controlling the incoming waves. We refer to such processes as coherent perfect chann...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-14422-9

    authors： Zhang X,Meng C,Yang Z

    更新日期：2017-10-24 00:00:00

  • Quantum error correction assisted by two-way noisy communication.

    abstract：:Pre-shared non-local entanglement dramatically simplifies and improves the performance of quantum error correction via entanglement-assisted quantum error-correcting codes (EAQECCs). However, even considering the noise in quantum communication only, the non-local sharing of a perfectly entangled pair is technically im...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep07203

    authors： Wang Z,Yu S,Fan H,Oh CH

    更新日期：2014-11-26 00:00:00

  • Investigation of thermal energy transport interface of hybrid graphene-carbon nanotube/polyethylene nanocomposites.

    abstract：:It is well known the thermal properties of three-dimensional (3-D) hybrid graphene (GR)-carbon nanotube (CNT) structures are not superior to that of the individual GR and CNT, however, the 3-D hybrid GR-CNT structures can effectively improve the thermal properties of polymer matrix. Therefore, understanding the therma...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-14710-4

    authors： Liu F,Liu X,Hu N,Ning H,Atobe S,Yan C,Mo F,Fu S,Zhang J,Wang Y,Mu X

    更新日期：2017-10-31 00:00:00

  • Aspirin might reduce the incidence of pancreatic cancer: A meta-analysis of observational studies.

    abstract：:Although there is evidence that non-steroidal anti-inflammatory drugs (NSAIDs) might be able to prevent pancreatic cancer, the findings from epidemiological studies have been inconsistent. In this paper, we conducted a meta-analysis of observational studies to examine this possibility. We searched PubMed and Embase fo...

    journal_title：Scientific reports

    pub_type： 杂志文章,meta分析

    doi：10.1038/srep15460

    authors： Zhang YP,Wan YD,Sun YL,Li J,Zhu RT

    更新日期：2015-10-21 00:00:00

  • Melanoma cell-derived exosomes in plasma of melanoma patients suppress functions of immune effector cells.

    abstract：:Melanoma patients' plasma contains exosomes produced by malignant and normal cells. Plasma exosomes were isolated and separated by immunocapture into two fractions: melanoma cell-derived exosomes (MTEX) and normal cell-derived exosomes (non-MTEX). Immunosuppressive effects of MTEX on primary human immune cells were ev...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-56542-4

    authors： Sharma P,Diergaarde B,Ferrone S,Kirkwood JM,Whiteside TL

    更新日期：2020-01-09 00:00:00

  • Phylogenomic analysis of trichomycterid catfishes (Teleostei: Siluriformes) inferred from ultraconserved elements.

    abstract：:The family Trichomycteridae is one of the most diverse groups of freshwater catfishes in South and Central America with eight subfamilies, 41 genera and more than 300 valid species. Its members are widely distributed throughout South America, reaching Costa Rica in Central America and are recognized by extraordinary a...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-59519-w

    authors： Ochoa LE,Datovo A,DoNascimiento C,Roxo FF,Sabaj MH,Chang J,Melo BF,Silva GSC,Foresti F,Alfaro M,Oliveira C

    更新日期：2020-02-14 00:00:00