Correcting CRISPR for copy number.

Abstract:

:The CRISPR-Cas9 system enables global screens of gene function with high sensitivity and specificity, but off-target effects have been reported for CRISPR guide RNAs targeting genes that are amplified at high copy number. A new study describes a computational approach to correct for this copy number effect, increasing the specificity of CRIPSR screens to identify essential genes.

journal_name

Nat Genet

journal_title

Nature genetics

authors

Shen JP,Ideker T

doi

10.1038/ng.3994

subject

Has Abstract

pub_date

2017-11-29 00:00:00

pages

1674-1675

issue

12

eissn

1061-4036

issn

1546-1718

pii

ng.3994

journal_volume

49

pub_type

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