Abstract：
:Pluripotency can be induced in vitro from adult somatic mammalian cells by enforced expression of defined transcription factors regulating and initiating the pluripotency network. Despite the substantial advances over the last decade to improve the efficiency of direct reprogramming, exact mechanisms underlying the conversion into the pluripotent stem cell state are still vaguely understood. Several studies suggested that induced pluripotency follows reversed embryonic development. For somatic cells of mesodermal and endodermal origin that would require the transition through a Primitive streak-like state, which would necessarily require an Eomesodermin (Eomes) expressing intermediate. We analyzed reprogramming in human and mouse cells of mesodermal as well as ectodermal origin by thorough marker gene analyses in combination with genetic reporters, conditional loss of function and stable fate-labeling for the broad primitive streak marker Eomes. We unambiguously demonstrate that induced pluripotency is not dependent on a transient primitive streak-like stage and thus does not represent reversal of mesendodermal development in vivo.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Raab S,Klingenstein M,Möller A,Illing A,Tosic J,Breunig M,Kuales G,Linta L,Seufferlein T,Arnold SJ,Kleger A,Liebau Sdoi
10.1038/s41598-017-15187-xsubject
Has Abstractpub_date
2017-11-29 00:00:00pages
16543issue
1issn
2045-2322pii
10.1038/s41598-017-15187-xjournal_volume
7pub_type
杂志文章abstract：:Chemotherapy can cause cachexia, which consists of weight loss associated with muscle atrophy. The exact mechanisms underlying this skeletal muscle toxicity are largely unknown and co-therapies to attenuate chemotherapy-induced side effects are lacking. By using a rat model of cisplatin-induced cachexia, we here chara...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-13504-y
更新日期：2017-10-12 00:00:00
abstract：:Atomistic molecular dynamics simulations were performed with 13 non-peptidic neurotransmitters (NTs) in three different membrane environments. The results provide compelling evidence that NTs are divided into membrane-binding and membrane-nonbinding molecules. NTs adhere to the postsynaptic membrane surface whenever t...
journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2016-01-19 00:00:00
abstract：:A correction to this article has been published and is linked from the HTML version of this paper. The error has been fixed in the paper. ...
journal_title：Scientific reports
pub_type： 杂志文章,已发布勘误
doi：10.1038/s41598-017-18751-7
更新日期：2018-01-08 00:00:00
abstract：:Cardiac fibroblasts are present throughout the myocardium and are enriched in the microenvironment surrounding the ventricular conduction system (VCS). Several forms of arrhythmias are linked to VCS abnormalities, but it is still unclear whether VCS malformations are cardiomyocyte autonomous or could be linked to cros...
journal_title：Scientific reports
pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：Scientific reports
pub_type： 杂志文章
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journal_title：Scientific reports
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journal_title：Scientific reports
pub_type： 杂志文章
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2017-09-21 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2020-03-11 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2017-02-17 00:00:00
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journal_title：Scientific reports
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更新日期：2020-04-14 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2018-10-15 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2017-02-06 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2015-06-12 00:00:00
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journal_title：Scientific reports
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更新日期：2015-03-02 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2013-01-01 00:00:00
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更新日期：2017-06-20 00:00:00
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更新日期：2019-02-13 00:00:00
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journal_title：Scientific reports
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更新日期：2012-01-01 00:00:00
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journal_title：Scientific reports
pub_type： 临床试验,杂志文章
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journal_title：Scientific reports
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更新日期：2015-11-25 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2018-05-03 00:00:00
