Abstract:
:Activating transcription factor 4 (ATF4) is constitutively expressed in a variety of tissues, and regulates several pathological features associated with metabolic diseases such as non-alcoholic fatty liver diseases (NAFLD) and obesity. However, the role of ATF4 in animal model systems is poorly understood. To investigate ATF4 functions in zebrafish, we conditionally expressed ATF4 proteins, using a Tet-off transgenic system. We observed early-onset hyperlipidaemia and liver steatosis in ATF4 transgenic zebrafish (ATs) without doxycycline treatment (ATs - Dox). Oil Red O (ORO)-stained signals were predominant in the intravascular blood vessels and liver buds of larval ATs - Dox, indicating that ATF4 functionally promotes lipogenesis. Further, ATF4 overexpression accompanied the stimulation of the unfolded protein response. Therefore, adult ATs - Dox showed increased lipid accumulation, which led, in turn, to liver steatosis. Liver histology and ORO staining of ATs - Dox hepatocytes also indicated oxidative stress and induced NASH-like phenotypes. Moreover, ATF4 overexpression accelerated adipocyte differentiation via CCAAT enhancer binding protein-beta and peroxisome proliferator activated receptor-gamma inducible expression. ATs-Dox zebrafish showed increased weight gain with larger fat pads due to adipocyte hyperplasia. In this study, we report that ATF4 is a potential stimulator of lipid biosynthesis and adipogenesis in zebrafish.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Yeh KY,Lai CY,Lin CY,Hsu CC,Lo CP,Her GMdoi
10.1038/s41598-017-16587-9subject
Has Abstractpub_date
2017-11-27 00:00:00pages
16362issue
1issn
2045-2322pii
10.1038/s41598-017-16587-9journal_volume
7pub_type
杂志文章abstract::Early diagnosis of Parkinson's disease (PD) remains a challenge to date. New evidence highlights the potential clinical value of circulating trace amines (TAs) in early-stage PD and their involvement in disease progression. A new ultra performance chromatography mass spectrometry (UPLC-MS/MS) method was developed to q...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-42535-w
更新日期:2019-04-16 00:00:00
abstract::The production of tuberous roots is usually reduced by vigorous vegetative growth because of the competition for resource between the vegetative parts and reproductive organs. In this study, we conducted root pruning to examine the vigorous vegetative growth by regulating root growth, subsequently limiting vegetative ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-22586-1
更新日期:2018-03-13 00:00:00
abstract::Rice is the most important crop in the world, acting as the staple food for over half of the world's population. The evolutionary relationships of cultivated rice and its wild relatives have remained contentious and inconclusive. Here we report on the use of whole chloroplast sequences to elucidate the evolutionary an...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep13957
更新日期:2015-09-10 00:00:00
abstract::The conformational landscapes of p53 peptide variants and phage derived peptide (12/1) variants, all known to bind to MDM2, are studied using hamiltonian replica exchange molecular dynamics simulations. Complementing earlier observations, the current study suggests that the p53 peptides largely follow the 'conformatio...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-15930-4
更新日期:2017-11-15 00:00:00
abstract::Green fluorescent protein (GFP) is one of the most used reporter genes. We have used next-generation sequencing (NGS) to analyse the genetic diversity of a recombinant influenza A virus that expresses GFP and found a remarkable coverage dip in the GFP coding sequence. This coverage dip was present when virus-derived R...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep26314
更新日期:2016-05-19 00:00:00
abstract::Multi-drug resistant Pseudomonas aeruginosa has increased progressively and impedes further regression in mortality in burn patients. Such wound infections serve as bacterial reservoir for nosocomial infections and are associated with significant morbidity and costs. Anti-microbial polycationic dendrimers G3KL and G3R...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep22020
更新日期:2016-02-25 00:00:00
abstract::Nitrous oxide (N2O) is a potent greenhouse gas. In North China, vegetable fields are amended with high levels of N fertilizer and irrigation water, which causes massive N2O flux. The aim of this study was to determine the contribution of microbial processes to N2O production and characterize isotopic signature effects...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep29257
更新日期:2016-07-08 00:00:00
abstract::We set out to determine the usability of serum neurofilament light chain (sNfL), serum glial fibrillary acidic protein (sGFAP), and retinal parameters by using optical coherence tomography (OCT) as reliable biomarkers of the progression of oxaliplatin-induced peripheral neuropathy (OIPN). Forty-three patients schedule...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-64511-5
更新日期:2020-05-14 00:00:00
abstract::The effect of substrate surface roughening on the capacitance of Ni(OH)2/NiOOH nanowall array samples produced via chemical bath deposition for 2, 4, 6, 24 and 48 h on an as-received stainless steel substrate and the same substrate after sandblasting has been investigated. Symmetric cells were subjected to 120,000 cha...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-53365-1
更新日期:2019-11-14 00:00:00
abstract::MicroRNAs can promote translation of specific mRNAs in quiescent (G0) mammalian cells and immature Xenopus laevis oocytes. We report that microRNA-mediated upregulation of target mRNAs in oocytes is dependent on nuclear entry of the microRNA; cytoplasmically-injected microRNA repress target mRNAs. Components of the ac...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep00842
更新日期:2012-01-01 00:00:00
abstract::The differential conductance of graphene is shown to exhibit a zero-bias anomaly at low temperatures, arising from a suppression of the quantum corrections due to weak localization and electron interactions. A simple rescaling of these data, free of any adjustable parameters, shows that this anomaly exhibits a univers...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-62313-3
更新日期:2020-03-27 00:00:00
abstract::Marek's disease virus (MDV) is a preferred vector in the construction of recombinant vaccines. However, bivalent vaccine based on MDV that confers full protection against both very virulent Marek's and infectious bursal disease virus (IBDV) infections in chickens has not been produced. Here we developed a system utili...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep39263
更新日期:2016-12-16 00:00:00
abstract::Canine distemper virus (CDV) is the cause of a multisystem disease in domestic dogs and wild animals, infecting more than 20 carnivore and non-carnivore families and even infecting human cell lines in in vitro conditions. Phylogenetic classification based on the hemagglutinin gene shows 17 lineages with a phylogeograp...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-52345-9
更新日期:2019-10-31 00:00:00
abstract::Plutella xylostella has become the major lepidopteran pest of Brassica owing to its strong ability of resistance development to a wide range of insecticides. Destruxin A, a mycotoxin of entomopathogenic fungus, Metarhizium anisopliae, has broad-spectrum insecticidal effects. However, the interaction mechanism of destr...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-11298-7
更新日期:2017-09-08 00:00:00
abstract::The ATP-binding cassette transporter TAPL translocates polypeptides from the cytosol into the lysosomal lumen. TAPL can be divided into two functional units: coreTAPL, active in ATP-dependent peptide translocation, and the N-terminal membrane spanning domain, TMD0, responsible for cellular localization and interaction...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-33841-w
更新日期:2018-10-23 00:00:00
abstract::A variety of post-translational modifications of Plasmodium falciparum proteins, including phosphorylation and ubiquitination, are shown to have key regulatory roles during parasite development. NEDD8 is a ubiquitin-like modifier of cullin-RING E3 ubiquitin ligases, which regulates diverse cellular processes. Although...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-77001-5
更新日期:2020-11-19 00:00:00
abstract::Many established inflammation- and nutrition-related factors have been investigated as potential independent prognostic factors in various cancers, including the C-reactive protein/albumin ratio (CAR), lymphocyte/monocyte ratio (LMR), modified Glasgow prognostic score (mGPS), body mass index (BMI), and prognostic nutr...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-10328-8
更新日期:2017-08-25 00:00:00
abstract::It is generally assumed that an investment into cognitive abilities and their associated cost is particularly beneficial for long-lived species, as a prolonged lifespan allows to recoup the initial investment. However, ephemeral organisms possess astonishing cognitive abilities too. Invertebrates, for example, are cap...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-51652-5
更新日期:2019-10-23 00:00:00
abstract::Duchenne muscular dystrophy (DMD) is a disease marked by the development of skeletal muscle weakness and wasting. DMD results from mutations in the gene for the cytoskeletal protein dystrophin. The loss of dystrophin expression is not limited to muscle weakness but has multiple systemic consequences. Managing the nutr...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-31753-3
更新日期:2018-09-11 00:00:00
abstract::The mechanism whereby lactic acid bacteria extend the lifespan of Caenorhabditis elegans has previously been elucidated. However, the role of Weissella species has yet not been studied. We show that Weissella koreensis and Weissella cibaria significantly (p < 0.05) extend the lifespan of C. elegans compared with Esche...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep17128
更新日期:2015-11-25 00:00:00
abstract::Dehydroascorbate reductase (DHAR) is a key enzyme involved in the recycling of ascorbate, which catalyses the glutathione (GSH)-dependent reduction of oxidized ascorbate (dehydroascorbate, DHA). As a result, DHAR regenerates a pool of reduced ascorbate and detoxifies reactive oxygen species (ROS). In previous experime...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep19498
更新日期:2016-01-18 00:00:00
abstract::Humans interact with the oceans in diverse and profound ways. The scope, magnitude, footprint and ultimate cumulative impacts of human activities can threaten ocean ecosystems and have changed over time, resulting in new challenges and threats to marine ecosystems. A fundamental gap in understanding how humanity is af...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-47201-9
更新日期:2019-08-12 00:00:00
abstract::Whey protein concentrate (WPC) is an amino acid-rich supplement that has been shown to increase cellular antioxidant capacity. Mammalian target of rapamycin (mTOR) is a crucial regulator of signaling in mammalian cells, and serves as a therapeutic target for triple-negative breast cancer (TNBC). This study was designe...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-14159-5
更新日期:2017-11-21 00:00:00
abstract::Species of Pomacea, commonly known as apple snails, are native to South America, and have become widely distributed agricultural and environmental pests in southern China since their introduction in the 1980s. However, only since 2010 have researchers recognized that at least two species, P. canaliculata and P. macula...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-19000-7
更新日期:2018-01-19 00:00:00
abstract::Differential scanning fluorimetry is a popular method to estimate the stability of a protein in distinct buffer conditions by determining its 'melting point'. The method requires a temperature controlled fluorescence spectrometer or a RT-PCR machine. Here, we introduce a low-budget version of a microcontroller based h...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-19702-6
更新日期:2018-01-23 00:00:00
abstract::Adverse events during the prenatal and early postnatal period of life are associated with development of cardiovascular disease in adulthood. Prenatal exposure to excess testosterone (T) in sheep induces adverse reproductive and metabolic programming leading to polycystic ovarian syndrome, insulin resistance and hyper...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep28335
更新日期:2016-06-22 00:00:00
abstract::Automatically detecting and grading cancerous regions on radical prostatectomy (RP) sections facilitates graphical and quantitative pathology reporting, potentially benefitting post-surgery prognosis, recurrence prediction, and treatment planning after RP. Promising results for detecting and grading prostate cancer on...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-66849-2
更新日期:2020-06-18 00:00:00
abstract::This corrects the article DOI: 10.1038/srep46667. ...
journal_title:Scientific reports
pub_type: 杂志文章,已发布勘误
doi:10.1038/srep46924
更新日期:2017-12-22 00:00:00
abstract::Aggregates of corundum crystals with skeletal to hopper morphology occur in pyroclastic rocks erupted from Cretaceous basaltic volcanoes on Mt Carmel, N. Israel. The rapid growth of the crystals trapped volumes of the parental Al2O3-supersaturated melt; phenocrysts of tistarite (Ti2O3) in the trapped melts indicate cr...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-79739-4
更新日期:2021-01-28 00:00:00
abstract::Bilirubin neurotoxicity has been studied for decades and has been shown to affect various mechanisms via significant modulation of gene expression. This suggests that vital regulatory mechanisms of gene expression, such as epigenetic mechanisms, could play a role in bilirubin neurotoxicity. Histone acetylation has rec...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-32106-w
更新日期:2018-09-12 00:00:00