Abstract:
:Break-induced replication (BIR) is a DNA double-strand break repair pathway that leads to genomic instabilities similar to those observed in cancer. BIR proceeds by a migrating bubble where asynchrony between leading and lagging strand synthesis leads to accumulation of long single-stranded DNA (ssDNA). It remains unknown how this ssDNA is prevented from unscheduled pairing with the template, which can lead to genomic instability. Here, we propose that uncontrolled Rad51 binding to this ssDNA promotes formation of toxic joint molecules that are counteracted by Srs2. First, Srs2 dislodges Rad51 from ssDNA preventing promiscuous strand invasions. Second, it dismantles toxic intermediates that have already formed. Rare survivors in the absence of Srs2 rely on structure-specific endonucleases, Mus81 and Yen1, that resolve toxic joint-molecules. Overall, we uncover a new feature of BIR and propose that tight control of ssDNA accumulated during this process is essential to prevent its channeling into toxic structures threatening cell viability.
journal_name
Nat Communjournal_title
Nature communicationsauthors
Elango R,Sheng Z,Jackson J,DeCata J,Ibrahim Y,Pham NT,Liang DH,Sakofsky CJ,Vindigni A,Lobachev KS,Ira G,Malkova Adoi
10.1038/s41467-017-01987-2subject
Has Abstractpub_date
2017-11-27 00:00:00pages
1790issue
1issn
2041-1723pii
10.1038/s41467-017-01987-2journal_volume
8pub_type
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