Abstract:
:During viral infections, some viruses subvert the host proteins to promote the translation or RNA replication with their protease-mediated cleavage. Poly (A)-binding protein (PABP) is a target for several RNA viruses; however, the impact of duck hepatitis A virus (DHAV) on PABP remains unknown. In this study, we demonstrated for the first time that DHAV infection stimulates a decrease in endogenous PABP and generates two cleavage fragments. On the basis of in vitro cleavage assays, an accumulation of PABP cleavage fragments was detected in duck embryo fibroblast (DEF) cell extracts incubated with functional DHAV 3C protease. In addition, DHAV 3C protease was sufficient for the cleavage of recombinant PABP without the assistance of other eukaryotic cellular cofactors. Furthermore, using site-directed mutagenesis, our data demonstrated a 3C protease cleavage site located between Q367 and G368 in duck PABP. Moreover, the knockdown of PABP inhibited the production of viral RNA, and the C-terminal domain of PABP caused a reduction in viral replication compared to the N-terminal domain. Taken together, these findings suggested that DHAV 3C protease mediates the cleavage of PABP, which may be a strategy to manipulate viral replication.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Sun D,Wang M,Wen X,Cheng A,Jia R,Sun K,Yang Q,Wu Y,Zhu D,Chen S,Liu M,Zhao X,Chen Xdoi
10.1038/s41598-017-16484-1subject
Has Abstractpub_date
2017-11-24 00:00:00pages
16261issue
1issn
2045-2322pii
10.1038/s41598-017-16484-1journal_volume
7pub_type
杂志文章abstract::Switching magnetizations with femtosecond circularly polarized lasers may have revolutionary impacts on magnetic data storage and relevant applications. Achievements in ferrimagnetic and ferromagnetic materials of various structures strongly imply a general phenomenon of fundamental atom-laser interaction. Rotating an...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep41294
更新日期:2017-01-24 00:00:00
abstract::Melting is considered to be one of the most fundamental problems in physical science. Generally, dimensionality plays an important role in melting. In three-dimension, it's well known that a crystal melts directly into a liquid via a first-order transition. In two-dimension (2D), however, the melting process has been ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep24056
更新日期:2016-04-07 00:00:00
abstract::The present study applied the PacBio single molecule, real-time sequencing technology (SMRT) in evaluating the quality of silage production. Specifically, we produced four types of Medicago sativa silages by using four different lactic acid bacteria-based additives (AD-I, AD-II, AD-III and AD-IV). We monitored the cha...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep28358
更新日期:2016-06-24 00:00:00
abstract::The concept of multiscale architectured materials is established using composition and grain size gradients. Composition-gradient nanostructured materials are produced from coarse grained interstitial free steels via carburization and high-pressure torsion. Quantitative analyses of the dislocation density using X-ray ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep26590
更新日期:2016-05-27 00:00:00
abstract::The design of novel exciplex-forming co-host materials provides new opportunities to achieve high device performance of organic light emitting diodes (OLEDs), including high efficiency, low driving voltage and low efficiency roll-off. Here, we report a comprehensive study of exciplex-forming co-host system in OLEDs in...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-12059-2
更新日期:2017-09-20 00:00:00
abstract::The effect of substrate surface roughening on the capacitance of Ni(OH)2/NiOOH nanowall array samples produced via chemical bath deposition for 2, 4, 6, 24 and 48 h on an as-received stainless steel substrate and the same substrate after sandblasting has been investigated. Symmetric cells were subjected to 120,000 cha...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-53365-1
更新日期:2019-11-14 00:00:00
abstract::Hox proteins are transcription factors and key regulators of segmental identity along the anterior posterior axis across all bilaterian animals. Despite decades of research, the mechanisms by which Hox proteins select and regulate their targets remain elusive. We have carried out whole-genome ChIP-chip experiments to ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep00205
更新日期:2011-01-01 00:00:00
abstract::The monitoring and early detection of abnormalities or variations in the cardiac cycle functionality are very critical practices and have significant impact on the prevention of heart diseases and their associated complications. Currently, in the field of biomedical engineering, there is a growing need for devices cap...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep37093
更新日期:2016-11-17 00:00:00
abstract::Biomarkers suitable for early diagnosis and monitoring disease progression are the cornerstone of developing disease-modifying treatments for neurodegenerative diseases such as Parkinson's disease (PD). Besides motor complications, PD is also characterized by deficits in visual processing. Here, we investigate how vir...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-68808-3
更新日期:2020-07-17 00:00:00
abstract::Graphene oxide-iron oxide (GO-Fe3O4) nanocomposites were synthesised by co-precipitating iron salts onto GO sheets in basic solution. The results showed that formation of two distinct structures was dependent upon the GO loading. The first structure corresponds to a low GO loading up to 10 wt%, associated with the ben...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep04594
更新日期:2014-04-04 00:00:00
abstract::Kmmig1 as a disrupted mutant of MIG1 encoding a regulator for glucose repression in Kluyveromyces marxianus exhibits a histidine-auxotrophic phenotype. Genome-wide expression analysis revealed that only HIS4 in seven HIS genes for histidine biosynthesis was down-regulated in Kmmig1. Consistently, introduction of HIS4 ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-46411-5
更新日期:2019-07-09 00:00:00
abstract::Ultrafast betatron x-ray emission from electron oscillations in laser wakefield acceleration (LWFA) has been widely investigated as a promising source. Betatron x-rays are usually produced via self-injected electron beams, which are not controllable and are not optimized for x-ray yields. Here, we present a new method...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep27633
更新日期:2016-06-08 00:00:00
abstract::The animal gut microbiota evolves quickly towards a complex community and plays crucial roles in its host's health and development. Factors such as host genetics and environmental changes are regarded as important for controlling the dynamics of animal gut microbiota. Migratory animals are an important group for study...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-64570-8
更新日期:2020-05-05 00:00:00
abstract::Constrained by the diffraction limit, a lens can only resolve features larger than half of the incident wavelength owing to the decaying nature of evanescent waves. Several novel devices have been proposed, for example, superlenses and hyperlenses to break this limit. In this work, we present a flat hyperlens composed...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-62243-0
更新日期:2020-03-24 00:00:00
abstract::We formerly developed an automatic colony count system based on the time-lapse shadow image analysis (TSIA). Here this system has been upgraded and applied to practical rapid decision. A microbial sample was spread on/in an agar plate with 90 mm in diameter as homogeneously as possible. We could obtain the results wit...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep10061
更新日期:2015-05-15 00:00:00
abstract::Recently, development of Adeno-associated virus (AAV) vectors has been focusing on expanding the genetic diversity of vectors from existing sequences via directed evolution or epitope remapping. Apart from intelligent design, AAV isolation from natural sources remains an important source of new AAVs with unique biolog...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep06644
更新日期:2014-10-22 00:00:00
abstract::There is emerging evidence that microRNAs (miRNAs) dysregulation is involved in the genesis and the progression of Prostate Cancer (PCa), thus potentially increasing their use in urological clinical practice. This is the first pilot study which utilizes Illumina Deep Sequencing to examine the entire miRNAs spectrum ex...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-24236-y
更新日期:2018-05-08 00:00:00
abstract::The microRNA (miR)-183/96/182 cluster plays important roles in the development and functions of sensory organs, including the inner ear. Point-mutations in the seed sequence of miR-96 result in non-syndromic hearing loss in both mice and humans. However, the lack of a functionally null mutant has hampered the evaluati...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-36894-z
更新日期:2018-12-21 00:00:00
abstract::Endocannabinoids are amphiphilic molecules that play crucial neurophysiological functions acting as lipid messengers. Antagonists and knockdown of the classical CB1 and CB2 cannabinoid receptors do not completely abolish many endocannabinoid activities, supporting the idea of a mechanism independent of receptors whose...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep41362
更新日期:2017-01-27 00:00:00
abstract::IgLONs are members of the immunoglobulin superfamily of cell adhesion proteins implicated in the process of neuronal outgrowth, cell adhesion and subdomain target recognition. IgLONs form homophilic and heterophilic complexes on the cell surface that repress or promote growth depending on the neuronal population, the ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-08315-0
更新日期:2017-08-11 00:00:00
abstract::In this work, we report our study of protein expression in rat peri-infarct tissue, 48 h after the induction of permanent focal cerebral ischemia. Two proteomic approaches, gel electrophoresis with mass spectrometry and combined fractional diagonal chromatography (COFRADIC), were performed using tissue samples from th...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep12030
更新日期:2015-07-08 00:00:00
abstract::Growth factors have great therapeutic potential for various disease therapy and tissue engineering applications. However, their clinical efficacy is hampered by low bioavailability, rapid degradation in vivo and non-specific biodistribution. Nanoparticle based delivery systems are being evaluated to overcome these lim...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-14040-5
更新日期:2017-10-30 00:00:00
abstract::Cortical gyrification of the brain represents the folding characteristic of the cerebral cortex. How the brain cortical gyrification changes from childhood to old age in healthy human subjects is still unclear. Additionally, studies have shown regional gyrification alterations in patients with major psychiatric disord...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-00582-1
更新日期:2017-03-30 00:00:00
abstract::Non-fibrillar protein aggregates that appear in the earlier stages of amyloid fibril formation are sometimes considered to play a key role in amyloid nucleation; however, the structural features of these aggregates currently remain unclear. We herein identified a characteristic pathway of fibril formation by human ins...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-18390-y
更新日期:2018-01-08 00:00:00
abstract::Alternative tillage and rice establishment options should aim at less water and labor to produce similar or improved yields compared with traditional puddled-transplanted rice cultivation. The relative performance of these practices in terms of yield, water input, and economics varies across rice-growing regions. A gl...
journal_title:Scientific reports
pub_type: 杂志文章,meta分析
doi:10.1038/s41598-017-09742-9
更新日期:2017-08-24 00:00:00
abstract::The key to arthritis management is early diagnosis and treatment to prevent further joint destruction and maximize functional ability. Osteoarthritis (OA) and rheumatoid arthritis (RA) are two common types of arthritis that the primary care provider must differentiate, in terms of diagnosis and treatment. Effective an...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-35473-6
更新日期:2018-11-20 00:00:00
abstract::The mammalian retina encodes the visual world in action potentials generated by 20-50 functionally and anatomically-distinct types of retinal ganglion cell (RGC). Individual RGC types receive synaptic input from distinct presynaptic circuits; therefore, their responsiveness to specific features in the visual scene ari...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-45048-8
更新日期:2019-06-18 00:00:00
abstract::We found a novel spontaneous mouse mutant with depigmentation in the ventral body, which we called White Spotting (WS) mouse. Genetic investigation revealed deletion of a > 1.2-Mb genomic region containing nine genes (Kit, Kdr, Srd5a3, Tmeme165, Clock, Pdcl2, Nmu, Exoc1, and Cep135). We designated this mutant allele K...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep13632
更新日期:2015-09-08 00:00:00
abstract::This study investigates the effects of aircraft cabin pressure on intracranial pressure (ICP) elevation of a pneumocephalus patient. We propose an experimental setup that simulates the intracranial hydrodynamics of a pneumocephalus patient during flight. It consists of an acrylic box (skull), air-filled balloon [intra...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-70614-w
更新日期:2020-08-12 00:00:00
abstract::Soil respiration (Rs) is an important source of atmospheric CO2 flux and is sensitive to changes in soil nutrient and water contents. Despite extensive studies on the effects of enhanced atmospheric nitrogen (N) deposition and changes in precipitation (P) on Rs, few studies have taken into account the effects of inter...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-34969-5
更新日期:2018-11-08 00:00:00