Abstract:
:γδT cells constitute a small proportion of lymphocytes in peripheral blood. Unlike αβT cells, the anti-tumor activities are exerted through several different pathways in a MHC-unrestricted manner. Thus, immunotherapy using γδT cells is considered to be effective for various types of cancer. Occasionally, however, ex vivo expanded cells are not as effective as expected due to cell exhaustion. To overcome the issue of T-cell exhaustion, researchers have generated induced pluripotent stem cells (iPSCs) that harbor the same T-cell receptor (TCR) genes as their original T-cells, which provide nearly limitless sources for antigen-specific cytotoxic T lymphocytes (CTLs). However, these technologies have focused on αβT cells and require a population of antigen-specific CTLs, which are purified by cell sorting with HLA-peptide multimer, as the origin of iPS cells. In the present study, we aimed to develop an efficient and convenient system for generating iPSCs that harbor rearrangements of the TCRG and TCRD gene regions (γδT-iPSCs) without cell-sorting. We stimulated human whole peripheral blood mononuclear cell (PBMC) culture using Interleukin-2 and Zoledronate to activate γδT cells. Gene transfer into those cells with the Sendai virus vector resulted in γδT cell-dominant expression of exogenous genes. The introduction of reprogramming factors into the stimulated PBMC culture allowed us to establish iPSC lines. Around 70% of the established lines carried rearrangements at the TCRG and TCRD gene locus. The γδT-iPSCs could differentiate into hematopoietic progenitors. Our technology will pave the way for new avenues toward novel immunotherapy that can be applied for various types of cancer. Stem Cells Translational Medicine 2018;7:34-44.
journal_name
Stem Cells Transl Medjournal_title
Stem cells translational medicineauthors
Watanabe D,Koyanagi-Aoi M,Taniguchi-Ikeda M,Yoshida Y,Azuma T,Aoi Tdoi
10.1002/sctm.17-0021subject
Has Abstractpub_date
2018-01-01 00:00:00pages
34-44issue
1eissn
2157-6564issn
2157-6580journal_volume
7pub_type
杂志文章abstract::The potential of autologous cell-based therapies including those using multipotent mesenchymal stromal cells (MSCs) is being investigated for multiple sclerosis (MS) and other neurological conditions. However, the phenotype of MSC in neurological diseases has not been fully characterized. We have previously shown that...
journal_title:Stem cells translational medicine
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pub_type: 杂志文章,评审
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journal_title:Stem cells translational medicine
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journal_title:Stem cells translational medicine
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journal_title:Stem cells translational medicine
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journal_title:Stem cells translational medicine
pub_type: 杂志文章
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journal_title:Stem cells translational medicine
pub_type: 杂志文章,评审
doi:10.5966/sctm.2012-0030
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journal_title:Stem cells translational medicine
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journal_title:Stem cells translational medicine
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journal_title:Stem cells translational medicine
pub_type: 杂志文章
doi:10.1002/sctm.20-0227
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journal_title:Stem cells translational medicine
pub_type: 杂志文章
doi:10.5966/sctm.2012-0155
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journal_title:Stem cells translational medicine
pub_type: 杂志文章
doi:10.1002/sctm.18-0216
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journal_title:Stem cells translational medicine
pub_type: 杂志文章
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journal_title:Stem cells translational medicine
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journal_title:Stem cells translational medicine
pub_type: 杂志文章
doi:10.1002/sctm.17-0086
更新日期:2018-02-01 00:00:00
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journal_title:Stem cells translational medicine
pub_type: 杂志文章
doi:10.1002/sctm.19-0114
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journal_title:Stem cells translational medicine
pub_type: 临床试验,杂志文章
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更新日期:2017-08-01 00:00:00
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journal_title:Stem cells translational medicine
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journal_title:Stem cells translational medicine
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journal_title:Stem cells translational medicine
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journal_title:Stem cells translational medicine
pub_type: 杂志文章,评审
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abstract::Perivascular stem cells (PSCs) are the natural ancestors of mesenchymal stem cells (MSCs) and are the stem cells responsible for homeostasis and repair in vivo. Prospectively identified and isolated PSCs have demonstrated increased plasticity and osteogenic potential. Cells from the infrapatellar fat pad (IFP) have de...
journal_title:Stem cells translational medicine
pub_type: 杂志文章
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journal_title:Stem cells translational medicine
pub_type: 杂志文章
doi:10.1002/sctm.18-0129
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journal_title:Stem cells translational medicine
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journal_title:Stem cells translational medicine
pub_type: 杂志文章,评审
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