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Andy's Algorithms: new automated digital image analysis pipelines for FIJI.

Abstract：

:Quantification of cellular antigens and their interactions via antibody-based detection methods are widely used in scientific research. Accurate high-throughput quantitation of these assays using general image analysis software can be time consuming and challenging, particularly when attempted by users with limited image processing and analysis knowledge. To overcome this, we have designed Andy's Algorithms, a series of automated image analysis pipelines for FIJI, that permits rapid, accurate and reproducible batch-processing of 3,3'-diaminobenzidine (DAB) immunohistochemistry, proximity ligation assays (PLAs) and other common assays. Andy's Algorithms incorporates a step-by-step tutorial and optimization pipeline to make batch image analysis simple for the untrained user and adaptable across laboratories. Andy's algorithms provide a simpler, faster, standardized work flow compared to existing programs, while offering equivalent performance and additional features, in a free to use open-source application of FIJI. Andy's Algorithms are available at GitHub, publicly accessed at https://github.com/andlaw1841/Andy-s-Algorithm .

journal_name

Sci Rep

journal_title

Scientific reports

authors

Law AMK,Yin JXM,Castillo L,Young AIJ,Piggin C,Rogers S,Caldon CE,Burgess A,Millar EKA,O'Toole SA,Gallego-Ortega D,Ormandy CJ,Oakes SR

doi

10.1038/s41598-017-15885-6

subject

Has Abstract

pub_date

2017-11-16 00:00:00

pages

15717

issue

1

issn

2045-2322

pii

10.1038/s41598-017-15885-6

journal_volume

7

pub_type

杂志文章

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