当前位置： SCI文献检索 > Scientific Reports期刊下所有文献 > Structural Basis for DNA Recognition of a Single-stranded DNA-binding Protein from Enterobacter Phage Enc34.

Structural Basis for DNA Recognition of a Single-stranded DNA-binding Protein from Enterobacter Phage Enc34.

Abstract：

:Modern DNA sequencing capabilities have led to the discovery of a large number of new bacteriophage genomes, which are a rich source of novel proteins with an unidentified biological role. The genome of Enterobacter cancerogenus bacteriophage Enc34 contains several proteins of unknown function that are nevertheless conserved among distantly related phages. Here, we report the crystal structure of a conserved Enc34 replication protein ORF6 which contains a domain of unknown function DUF2815. Despite the low (~15%) sequence identity, the Enc34 ORF6 structurally resembles the gene 2.5 protein from bacteriophage T7, and likewise is a single-stranded DNA (ssDNA)-binding protein (SSB) that consists of a variation of the oligosaccharide/oligonucleotide-binding (OB)-fold and an unstructured C-terminal segment. We further report the crystal structure of a C-terminally truncated ORF6 in complex with an ssDNA oligonucleotide that reveals a DNA-binding mode involving two aromatic stacks and multiple electrostatic interactions, with implications for a common ssDNA recognition mechanism for all T7-type SSBs.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Cernooka E,Rumnieks J,Tars K,Kazaks A

doi

10.1038/s41598-017-15774-y

subject

Has Abstract

pub_date

2017-11-14 00:00:00

pages

15529

issue

1

issn

2045-2322

pii

10.1038/s41598-017-15774-y

journal_volume

7

pub_type

杂志文章

相关文献

Scientific Reports文献大全
  • Erratum: Daytime warming has stronger negative effects on soil nematodes than night-time warming.

    abstract：:This corrects the article DOI: 10.1038/srep44888. ...

    journal_title：Scientific reports

    pub_type： 杂志文章,已发布勘误

    doi：10.1038/srep46881

    authors： Yan X,Wang K,Song L,Wang X,Wu D

    更新日期：2017-08-29 00:00:00

  • Silicon-core glass fibres as microwire radial-junction solar cells.

    abstract：:Vertically aligned radial-junction solar cell designs offer potential improvements over planar geometries, as carrier generation occurs close to the junction for all absorption depths, but most production methods still require a single crystal substrate. Here, we report on the fabrication of such solar cells from poly...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep06283

    authors： Martinsen FA,Smeltzer BK,Nord M,Hawkins T,Ballato J,Gibson UJ

    更新日期：2014-09-04 00:00:00

  • BALB/c mice infected with DENV-2 strain 66985 by the intravenous route display injury in the central nervous system.

    abstract：:Dengue is a mild flu-like arboviral illness caused by dengue virus (DENV) that occurs in tropical and subtropical countries. An increasing number of reports have been indicating that dengue is also associated to neurological manifestations, however, little is known regarding the neuropathogenesis of the disease. Here,...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-28137-y

    authors： Salomão NG,Rabelo K,Póvoa TF,Alves AMB,da Costa SM,Gonçalves AJS,Amorim JF,Azevedo AS,Nunes PCG,Basílio-de-Oliveira CA,Basílio-de-Oliveira RP,Geraldo LHM,Fonseca CG,Lima FRS,Mohana-Borges R,Silva EM,Dos Santos FB,Olivei

    更新日期：2018-06-27 00:00:00

  • Safety profiles of terahertz scanning in ophthalmology.

    abstract：:Terahertz (THz) technology has emerged recently as a potential novel imaging modality in biomedical fields, including ophthalmology. However, the ocular biological responses after THz electromagnetic exposure have not been investigated. We conducted a rabbit study to evaluate the safety profiles of THz scanning on eye...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-021-82103-9

    authors： Liu YC,Ke L,Yang SWQ,Nan Z,Teo EPW,Lwin NC,Lin MT,Lee IXY,Chan AS,Schmetterer L,Mehta JS

    更新日期：2021-01-28 00:00:00

  • Search for efficient inhibitors of myotoxic activity induced by ophidian phospholipase A2-like proteins using functional, structural and bioinformatics approaches.

    abstract：:Ophidian accidents are considered an important neglected tropical disease by the World Health Organization. Particularly in Latin America, Bothrops snakes are responsible for the majority of the snakebite envenomings that are not efficiently treated by conventional serum therapy. Thus, the search for simple and effici...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-36839-6

    authors： Salvador GHM,Cardoso FF,Gomes AA,Cavalcante WLG,Gallacci M,Fontes MRM

    更新日期：2019-01-24 00:00:00

  • Molecular evidence of Chlamydia pecorum and arthropod-associated Chlamydiae in an expanded range of marsupials.

    abstract：:The order Chlamydiales are biphasic intracellular bacterial pathogens infecting humans and domesticated animals. Wildlife infections have also been reported, with the most studied example being Chlamydia pecorum infections in the koala, an iconic Australian marsupial. In koalas, molecular evidence suggests that spill-...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-13164-y

    authors： Burnard D,Huston WM,Webb JK,Jelocnik M,Reiss A,Gillett A,Fitzgibbon S,Carver S,Carrucan J,Flanagan C,Timms P,Polkinghorne A

    更新日期：2017-10-09 00:00:00

  • Identification of a CD133-CD55- population functions as a fetal common skeletal progenitor.

    abstract：:In this study, we identified a CD105+CD90.1-CD133-CD55- (CD133-CD55-) population in the fetal skeletal element that can generate bone and bone marrow. Besides osteoblasts and chondrocytes, the CD133-CD55- common progenitors can give rise to marrow reticular stromal cells and perivascular mesenchymal progenitors sugges...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep38632

    authors： Weng L,Hu X,Kumar B,Garcia M,Todorov I,Jung X,Marcucci G,Forman SJ,Chen CC

    更新日期：2016-12-08 00:00:00

  • Neurite regrowth stimulation by a red-light spot focused on the neuronal cell soma following blue light-induced retraction.

    abstract：:The interaction of light with biological tissues has been considered for various therapeutic applications. Light-induced neurite growth has the potential to be a clinically useful technique for neuron repair. However, most previous studies used either a large illumination area to accelerate overall neurite growth or e...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-54687-w

    authors： Kao YC,Liao YC,Cheng PL,Lee CH

    更新日期：2019-12-03 00:00:00

  • Development and physiological effects of an artificial diet for Wolbachia-infected Aedes aegypti.

    abstract：:The endosymbiotic bacterium Wolbachia spreads rapidly through populations of Aedes aegypti mosquitoes, and strongly inhibits infection with key human pathogens including the dengue and Zika viruses. Mosquito control programs aimed at limiting transmission of these viruses are ongoing in multiple countries, yet there i...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-16045-6

    authors： Dutra HLC,Rodrigues SL,Mansur SB,de Oliveira SP,Caragata EP,Moreira LA

    更新日期：2017-11-16 00:00:00

  • Neuroprotection mediated by inhibition of calpain during acute viral encephalitis.

    abstract：:Neurologic complications associated with viral encephalitis, including seizures and cognitive impairment, are a global health issue, especially in children. We previously showed that hippocampal injury during acute picornavirus infection in mice is associated with calpain activation and is the result of neuronal death...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep28699

    authors： Howe CL,LaFrance-Corey RG,Mirchia K,Sauer BM,McGovern RM,Reid JM,Buenz EJ

    更新日期：2016-06-27 00:00:00

  • Tomato plants rather than fertilizers drive microbial community structure in horticultural growing media.

    abstract：:Synthetic fertilizer production is associated with a high environmental footprint, as compounds typically dissolve rapidly leaching emissions to the atmosphere or surface waters. We tested two recovered nutrients with slower release patterns, as promising alternatives for synthetic fertilizers: struvite and a commerci...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-45290-0

    authors： Grunert O,Robles-Aguilar AA,Hernandez-Sanabria E,Schrey SD,Reheul D,Van Labeke MC,Vlaeminck SE,Vandekerckhove TGL,Mysara M,Monsieurs P,Temperton VM,Boon N,Jablonowski ND

    更新日期：2019-07-02 00:00:00

  • Protein docking refinement by convex underestimation in the low-dimensional subspace of encounter complexes.

    abstract：:We propose a novel stochastic global optimization algorithm with applications to the refinement stage of protein docking prediction methods. Our approach can process conformations sampled from multiple clusters, each roughly corresponding to a different binding energy funnel. These clusters are obtained using a densit...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-23982-3

    authors： Zarbafian S,Moghadasi M,Roshandelpoor A,Nan F,Li K,Vakli P,Vajda S,Kozakov D,Paschalidis IC

    更新日期：2018-04-12 00:00:00

  • Chiral Separation by Flows: The Role of Flow Symmetry and Dimensionality.

    abstract：:Separation of enantiomers by flows is a promising chiral resolution method using cost-effective microfluidics. Notwithstanding a number of experimental and numerical studies, a fundamental understanding still remains elusive, and an important question as to whether it is possible to specify common physical properties ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep35144

    authors： Ro S,Yi J,Kim YW

    更新日期：2016-10-14 00:00:00

  • Crystallographic anisotropy of the resistivity size effect in single crystal tungsten nanowires.

    abstract：:This work demonstrates an anisotropic increase in resistivity with decreasing width in single crystal tungsten (W) nanowires having a height of 21 nm. Nanowire-widths were in the range of 15-451 nm, with the anisotropy observed for widths below 50 nm. The longitudinal directions of the nanowires coincided with the <10...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep02591

    authors： Choi D,Moneck M,Liu X,Oh SJ,Kagan CR,Coffey KR,Barmak K

    更新日期：2013-01-01 00:00:00

  • Performance of Azure-winged magpies in Aesop's fable paradigm.

    abstract：:In this study, the improved Aesop's fable paradigm-a series of experiments originally used to test whether some animals understand the causality associated with water replacement-was used to explore the cognitive ability of Azure-winged magpies (Cyanopica cyanus). Experimental results on causal cue tasks showed that t...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-80452-5

    authors： Zhang Y,Yu C,Chen L,Li Z

    更新日期：2021-01-12 00:00:00

  • Millimeter-wave pulsed heating in vitro: cell mortality and heat shock response.

    abstract：:Millimeter wave (MMW)-induced heating represents a promising alternative for non-invasive hyperthermia of superficial skin cancer, such as melanoma. Pulsed MMW-induced heating of tumors allows for reaching high peak temperatures without overheating surrounding tissues. Herein, for the first time, we evaluate apoptotic...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-51731-7

    authors： Orlacchio R,Le Page Y,Le Dréan Y,Le Guével R,Sauleau R,Alekseev S,Zhadobov M

    更新日期：2019-10-24 00:00:00

  • Transcriptome and long noncoding RNA sequencing of three extracellular vesicle subtypes released from the human colon cancer LIM1863 cell line.

    abstract：:Previously we reported that LIM1863 colorectal cancer (CRC) cells secrete three distinct extracellular vesicle subtypes - two subpopulations of exosomes (apical EpCAM-Exos and basolateral A33-Exos) and shed microvesicles (sMVs) - with distinct protein and miRNA signatures. Here, we extend our omics approach to underst...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep38397

    authors： Chen M,Xu R,Ji H,Greening DW,Rai A,Izumikawa K,Ishikawa H,Takahashi N,Simpson RJ

    更新日期：2016-12-05 00:00:00

  • Determination and correction of persistent biases in quantum annealers.

    abstract：:Calibration of quantum computers is essential to the effective utilisation of their quantum resources. Specifically, the performance of quantum annealers is likely to be significantly impaired by noise in their programmable parameters, effectively misspecification of the computational problem to be solved, often resul...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep18628

    authors： Perdomo-Ortiz A,O'Gorman B,Fluegemann J,Biswas R,Smelyanskiy VN

    更新日期：2016-01-19 00:00:00

  • Gene expression profile changes in the jejunum of weaned piglets after oral administration of Lactobacillus or an antibiotic.

    abstract：:The small intestine plays an essential role in the health and well-being of animals. Previous studies have shown that Lactobacillus has a protective effect on intestinal morphology, intestinal epithelium integrity and appropriate maturation of gut-associated tissues. Here, gene expression in jejunum tissue of weaned p...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-16158-y

    authors： Zhang D,Shang T,Huang Y,Wang S,Liu H,Wang J,Wang Y,Ji H,Zhang R

    更新日期：2017-11-17 00:00:00

  • Regulation of the β-hemolysin gene cluster of Streptococcus anginosus by CcpA.

    abstract：:Streptococcus anginosus is increasingly recognized as an opportunistic pathogen. However, our knowledge about virulence determinants in this species is scarce. One exception is the streptolysin-S (SLS) homologue responsible for the β-hemolytic phenotype of the S. anginosus type strain. In S. anginosus the expression o...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-27334-z

    authors： Bauer R,Mauerer S,Spellerberg B

    更新日期：2018-06-13 00:00:00

  • ATR-FTIR spectral discrimination between normal and tumorous mouse models of lymphoma and melanoma from serum samples.

    abstract：:This study presents, attenuated total reflection Fourier transforms infrared spectroscopy of dried serum samples in an effort to assess biochemical changes induced by non-Hodgkin's lymphoma and subcutaneous melanoma. An EL4 mouse model of non-Hodgkin lymphoma and a B16 mouse model of subcutaneous melanoma are used to ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-17027-4

    authors： Ghimire H,Venkataramani M,Bian Z,Liu Y,Perera AGU

    更新日期：2017-12-05 00:00:00

  • Activation of RSK by phosphomimetic substitution in the activation loop is prevented by structural constraints.

    abstract：:The activation of the majority of AGC kinases is regulated by two phosphorylation events on two conserved serine/threonine residues located on the activation loop and on the hydrophobic motif, respectively. In AGC kinase family, phosphomimetic substitutions with aspartate or glutamate, leading to constitutive activati...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-56937-3

    authors： Somale D,Di Nardo G,di Blasio L,Puliafito A,Vara-Messler M,Chiaverina G,Palmiero M,Monica V,Gilardi G,Primo L,Gagliardi PA

    更新日期：2020-01-17 00:00:00

  • Time course of bilateral microglial activation in a mouse model of laser-induced glaucoma.

    abstract：:Microglial activation is associated with glaucoma. In the model of unilateral laser-induced ocular hypertension (OHT), the time point at which the inflammatory process peaks remains unknown. Different time points (1, 3, 5, 8, and 15 d) were compared to analyze signs of microglial activation both in OHT and contralater...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-61848-9

    authors： Ramírez AI,de Hoz R,Fernández-Albarral JA,Salobrar-Garcia E,Rojas B,Valiente-Soriano FJ,Avilés-Trigueros M,Villegas-Pérez MP,Vidal-Sanz M,Triviño A,Ramírez JM,Salazar JJ

    更新日期：2020-03-17 00:00:00

  • Pre-symptomatic modified phytohormone profile is associated with lower phytoplasma titres in an Arabidopsis seor1ko line.

    abstract：:The proteins AtSEOR1 and AtSEOR2 occur as conjugates in the form of filaments in sieve elements of Arabidopsis thaliana. A reduced phytoplasma titre found in infected defective-mutant Atseor1ko plants in previous work raised the speculation that non-conjugated SEOR2 is involved in the phytohormone-mediated suppression...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-71660-0

    authors： Bernardini C,Pagliari L,De Rosa V,Almeida-Trapp M,Santi S,Martini M,Buoso S,Loschi A,Loi N,Chiesa F,Mithöfer A,van Bel AJE,Musetti R

    更新日期：2020-09-08 00:00:00

  • Anti-correlated cortical networks arise from spontaneous neuronal dynamics at slow timescales.

    abstract：:In the highly interconnected architectures of the cerebral cortex, recurrent intracortical loops disproportionately outnumber thalamo-cortical inputs. These networks are also capable of generating neuronal activity without feedforward sensory drive. It is unknown, however, what spatiotemporal patterns may be solely at...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-18097-0

    authors： Kodama NX,Feng T,Ullett JJ,Chiel HJ,Sivakumar SS,Galán RF

    更新日期：2018-01-12 00:00:00

  • High-throughput physical vapour deposition flexible thermoelectric generators.

    abstract：:Flexible thermoelectric generators (TEGs) can provide uninterrupted, green energy from body-heat, overcoming bulky battery configurations that limit the wearable-technologies market today. High-throughput production of flexible TEGs is currently dominated by printing techniques, limiting material choices and performan...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-41000-y

    authors： Morgan KA,Tang T,Zeimpekis I,Ravagli A,Craig C,Yao J,Feng Z,Yarmolich D,Barker C,Assender H,Hewak DW

    更新日期：2019-03-13 00:00:00

  • Non-classical crystallisation pathway directly observed for a pharmaceutical crystal via liquid phase electron microscopy.

    abstract：:Non-classical crystallisation (NCC) pathways are widely accepted, however there is conflicting evidence regarding the intermediate stages of crystallisation, how they manifest and further develop into crystals. Evidence from direct observations is especially lacking for small organic molecules, as distinguishing these...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-75937-2

    authors： Cookman J,Hamilton V,Hall SR,Bangert U

    更新日期：2020-11-05 00:00:00

  • A comparative study of sleep and diurnal patterns in house mouse (Mus musculus) and Spiny mouse (Acomys cahirinus).

    abstract：:Most published sleep studies use three species: human, house mouse, or Norway rat. The degree to which data from these species captures variability in mammalian sleep remains unclear. To gain insight into mammalian sleep diversity, we examined sleep architecture in the spiny basal murid rodent Acomys cahirinus. First,...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-67859-w

    authors： Wang C,Guerriero LE,Huffman DM,Ajwad AA,Brooks TC,Sunderam S,Seifert AW,O'Hara BF

    更新日期：2020-07-02 00:00:00

  • Transient desialylation in combination with a novel antithrombin deficiency causing a severe and recurrent thrombosis despite anticoagulation therapy.

    abstract：:An in-depth focused study of specific cases of patients with recurrent thrombosis may help to identify novel circumstances, genetic and acquired factors contributing to the development of this disorder. The aim of this study was to carry out a detailed and sequential analysis of samples from a patient suffering from e...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep44556

    authors： Revilla N,de la Morena-Barrio ME,Miñano A,López-Gálvez R,Toderici M,Padilla J,García-Avello Á,Lozano ML,Lefeber DJ,Corral J,Vicente V

    更新日期：2017-03-17 00:00:00

  • Lactobacillus casei reduces susceptibility to type 2 diabetes via microbiota-mediated body chloride ion influx.

    abstract：:Gut microbiota mediated low-grade inflammation is involved in the onset of type 2 diabetes (T2DM). In this study, we used a high fat sucrose (HFS) diet-induced pre-insulin resistance and a low dose-STZ HFS rat models to study the effect and mechanism of Lactobacillus casei Zhang in protecting against T2DM onset. Hyper...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep05654

    authors： Zhang Y,Guo X,Guo J,He Q,Li H,Song Y,Zhang H

    更新日期：2014-07-18 00:00:00