Abstract:
:The growing appreciation of the importance of long noncoding RNAs (lncRNAs), together with the awareness that some of these RNAs are associated with chromatin, has inspired the development of methods to detect their sites of interaction on a genome-wide scale at high resolution. Hybridization capture methods combine antisense oligonucleotide hybridization with enrichment of RNA from cross-linked chromatin extracts. These techniques have provided insight into lncRNA localization and the interactions of lncRNAs with protein to better understand biological roles of lncRNAs. Here, we review the core principles of hybridization capture methods, focusing on the three most commonly used protocols: capture hybridization analysis of RNA targets (CHART), chromatin isolation by RNA purification (ChIRP) and RNA affinity purification (RAP). We highlight the general principles of these techniques and discuss how differences in experimental procedures present distinct challenges to help researchers using these protocols or, more generally, interpreting the results of hybridization capture experiments.
journal_name
Brief Funct Genomicsjournal_title
Briefings in functional genomicsauthors
Machyna M,Simon MDdoi
10.1093/bfgp/elx038subject
Has Abstractpub_date
2018-03-01 00:00:00pages
96-103issue
2eissn
2041-2649issn
2041-2657pii
4604807journal_volume
17pub_type
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journal_title:Briefings in functional genomics
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journal_title:Briefings in functional genomics
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journal_title:Briefings in functional genomics
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journal_title:Briefings in functional genomics
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journal_title:Briefings in functional genomics
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journal_title:Briefings in functional genomics
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journal_title:Briefings in functional genomics
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journal_title:Briefings in functional genomics
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journal_title:Briefings in functional genomics
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journal_title:Briefings in functional genomics
pub_type: 杂志文章,评审
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更新日期:2014-09-01 00:00:00
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journal_title:Briefings in functional genomics
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更新日期:2013-01-01 00:00:00
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journal_title:Briefings in functional genomics
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journal_title:Briefings in functional genomics
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journal_title:Briefings in functional genomics
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journal_title:Briefings in functional genomics
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journal_title:Briefings in functional genomics
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journal_title:Briefings in functional genomics
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journal_title:Briefings in functional genomics
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journal_title:Briefings in functional genomics
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journal_title:Briefings in functional genomics
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journal_title:Briefings in functional genomics
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journal_title:Briefings in functional genomics
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journal_title:Briefings in functional genomics
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