Abstract：
:CRISPR-Cas genome editing technologies have revolutionized modern molecular biology by making targeted DNA edits simple and scalable. These technologies are developed by domesticating naturally occurring microbial adaptive immune systems that display wide diversity of functionality for targeted nucleic acid cleavage. Several CRISPR-Cas single effector enzymes have been characterized and engineered for use in mammalian cells. The unique properties of the single effector enzymes can make a critical difference in experimental use or targeting specificity. This review describes known single effector enzymes and discusses their use in genome engineering applications.
journal_name
ACS Chem Bioljournal_title
ACS chemical biologyauthors
Pyzocha NK,Chen Sdoi
10.1021/acschembio.7b00800subject
Has Abstractpub_date
2018-02-16 00:00:00pages
347-356issue
2eissn
1554-8929issn
1554-8937journal_volume
13pub_type
杂志文章,评审abstract：:Formylglycine (fGly) is a catalytically essential residue found almost exclusively in the active sites of type I sulfatases. Formed by post-translational oxidation of cysteine or serine side chains, this aldehyde-functionalized residue participates in a unique and highly efficient catalytic mechanism for sulfate ester...
journal_title：ACS chemical biology
pub_type： 杂志文章,评审
doi：10.1021/cb500897w
更新日期：2015-01-16 00:00:00
abstract：:Chloropupukeananin and chloropestolides are novel metabolites of the plant endophyte Pestalotiopsis fici, showing antimicrobial, antitumor, and anti-HIV activities. Their highly complex and unique skeletons were generated from the coisolated pestheic acid (1) and iso-A82775C (10) based on our previous studies. Here, w...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/acschembio.7b01059
更新日期：2018-03-16 00:00:00
abstract：:Many oncogenic mutants of the tumor suppressor p53 are conformationally unstable, including the frequently occurring Y220C mutant. We have previously developed several small-molecule stabilizers of this mutant. One of these molecules, PhiKan083, 1-(9-ethyl-9H-carbazole-3-yl)-N-methylmethanamine, binds to a mutation-in...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/acschembio.6b00315
更新日期：2016-08-19 00:00:00
abstract：:The Akt kinase family, consisting of three isoforms in humans, is a well-validated class of drug target. Through various screening campaigns in academics and pharmaceutical industries, several promising inhibitors have been developed to date. However, due to the mechanistic and structural similarities of Akt kinases, ...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/cb200388k
更新日期：2012-03-16 00:00:00
abstract：:Angiogenesis, the formation of new blood vessels, is implicated in a number of important human diseases, including cancer, diabetic retinopathy, and rheumatoid arthritis. To identify clinically useful angiogenesis inhibitors, we assembled and screened a library of mostly Food and Drug Administration-approved drugs for...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/cb600362d
更新日期：2007-04-24 00:00:00
abstract：:The oxidative addition of nitric oxide (NO) to a thiol, S-nitrosation, is a focus of studies on cyclic guanosine monophosphate (cGMP)-independent NO signaling. S-Nitrosation of the catalytic cysteine of the caspase proteases has important effects on apoptosis and consequently has received attention. Here we report on ...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/cb600393x
更新日期：2006-11-21 00:00:00
abstract：:Detection of metabolites and post-translational modifications can be achieved using the azide as a bioorthogonal chemical reporter. Once introduced into target biomolecules, either metabolically or through chemical modification, the azide can be tagged with probes using one of three highly selective reactions: the Sta...
journal_title：ACS chemical biology
pub_type： 信件
doi：10.1021/cb6003228
更新日期：2006-11-21 00:00:00
abstract：:New technology for the derivatization of peptide natural products is required for drug development. Despite the recent advances in the genome sequencing technique enabling us to search for the biosynthetic genes for wide variety of natural products, the technical methods to get access to them are limited. A class of R...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/acschembio.9b00330
更新日期：2019-06-21 00:00:00
abstract：:Current therapeutic interventions for both heart disease and heart failure are largely insufficient and associated with undesired side effects. Biomedical research has emphasized the role of sarcomeric protein function for the normal performance and energy efficiency of the heart, suggesting that directly targeting th...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/acschembio.0c00908
更新日期：2021-01-15 00:00:00
abstract：:Synthetic biology is the realization of systems with desired behavior using biological materials. A recent addition to the field is a bipartite consortium of the bacterium Escherichia coli in which each species harbors complementary gene circuits that actuate only when both are present above a critical density. This b...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/cb700256w
更新日期：2008-01-18 00:00:00
abstract：:Plasmodium falciparum thymidylate synthase-dihydrofolate reductase (TS-DHFR) is an essential enzyme in folate biosynthesis and a major malarial drug target. This bifunctional enzyme thus presents different design approaches for developing novel inhibitors against drug-resistant mutants. We performed a high-throughput ...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/cb8002804
更新日期：2009-01-16 00:00:00
abstract：:The use of nitric oxide (NO) as a signal for biofilm dispersal has been shown to increase the susceptibility of many biofilms to antibiotics, promoting their eradication. The delivery of NO to biofilms can be achieved by using NO donors with different kinetics and properties of NO release that can influence their effi...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/acschembio.7b00256
更新日期：2017-08-18 00:00:00
abstract：:The innate immune system provides the first line of defense against pathogens through the recognition of nonspecific patterns in RNA to protect the cell in a generalized way. The human RNA-activated protein kinase, PKR, is a dsRNA binding protein and an essential sensor in the innate immune response, which recognizes ...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/acschembio.6b00081
更新日期：2016-04-15 00:00:00
abstract：:The 3D structure of a protein is determined by the unique sequence of amino acid residues comprising the polypeptide chain. Sequence changes can cause protein misfolding, a potentially toxic phenomenon implicated in various neurodegenerative disorders. In a recent paper, translational misincorporation is proposed to b...
journal_title：ACS chemical biology
pub_type： 杂志文章,评审
doi：10.1021/cb6004068
更新日期：2006-10-24 00:00:00
abstract：:Despite being extensively characterized structurally and biochemically, the functional role of histone deacetylase 8 (HDAC8) has remained largely obscure due in part to a lack of known cellular substrates. Herein, we describe an unbiased approach using chemical tools in conjunction with sophisticated proteomics method...
journal_title：ACS chemical biology
pub_type： 信件
doi：10.1021/cb500492r
更新日期：2014-10-17 00:00:00
abstract：:Transcriptional activity is exquisitely sensitive to changes in promoter DNA topology. Transcription factors may therefore control gene activity by modulating the relative positioning of -10 and -35 promoter elements. The plant pathogen Pectobacterium atrosepticum, which causes soft rot in potatoes, must alter gene ex...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/acschembio.6b00168
更新日期：2016-07-15 00:00:00
abstract：:Available crystal structures of opioid receptors provide a high-resolution picture of ligand binding at the primary ("orthosteric") site, that is, the site targeted by endogenous ligands. Recently, positive allosteric modulators of opioid receptors have also been discovered, but their modes of binding and action remai...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/acschembio.5b00712
更新日期：2016-05-20 00:00:00
abstract：:Allosteric inhibitors can be more difficult to optimize without an understanding of how their binding influences the conformational motions of the target. Here, we used an integrated computational and experimental approach to probe the molecular mechanism of an allosteric inhibitor of heat shock protein 70 (Hsp70). Th...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/acschembio.8b00712
更新日期：2018-11-16 00:00:00
abstract：:Fatty acylation or lipid modification of proteins controls their cellular activation and diverse roles in physiology. It mediates protein-protein and protein-membrane interactions and plays an important role in regulating cellular signaling pathways. Currently, there is need for visualizing lipid modifications of prot...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/cb900085z
更新日期：2009-07-17 00:00:00
abstract：:Recent advances in mass spectrometry (MS)-based proteomics allow the identification and quantitation of thousands of posttranslational modification (PTM) sites in a single experiment. This follows from the development of more effective class enrichment strategies, new high performance instrumentation and bioinformatic...
journal_title：ACS chemical biology
pub_type： 杂志文章,评审
doi：10.1021/cb500904b
更新日期：2015-01-16 00:00:00
abstract：:Understanding the complex biochemical mechanisms that underlie the regulation, toxicity, and protein binding of metal ions requires the ability to analyze the metal content of individual proteins in complex mixtures. In this issue of ACS Chemical Biology, a technique combining gel electrophoresis with synchrotron X-ra...
journal_title：ACS chemical biology
pub_type： 评论,杂志文章
doi：10.1021/cb100145z
更新日期：2010-06-18 00:00:00
abstract：:Membrane curvature and lipid composition regulates important biological processes within a cell. Currently, several proteins have been reported to sense and/or induce membrane curvatures, e.g., Synaptotagmin-1 and Amphiphysin. However, the large protein scaffold of these curvature sensors limits their applications in ...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/cb300429e
更新日期：2013-01-18 00:00:00
abstract：:Designed second mitochondrial activator of caspases (Smac) mimetics based on an accessible [7,5]-bicyclic scaffold bind to and antagonize protein interactions involving the inhibitor of apoptosis (IAP) proteins, X-chromosome-linked IAP (XIAP), melanoma IAP (ML-IAP), and c-IAPs 1 and 2 (cIAP1 and cIAP2). The design rat...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/cb600276q
更新日期：2006-09-19 00:00:00
abstract：:It has been known for nearly a half century that human tumors, including those derived from the nervous system such as glioblastomas, medulloblastoma, and neuroblastomas are much more sensitive than normal tissues to l-methionine (l-Met) starvation. More recently, systemic l-Met depletion by administration of Pseudomo...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/cb300335j
更新日期：2012-11-16 00:00:00
abstract：:Many cellular processes are regulated by posttranslational modifications that are recognized by specific domains in protein binding partners. These interactions are often weak, thus allowing a highly dynamic and combinatorial regulatory network of protein-protein interactions. We report an efficient strategy that over...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/cb400723j
更新日期：2014-02-21 00:00:00
abstract：:The gibberellin (GA) phytohormones are produced not only by plants but also by fungi and bacteria. Previous characterization of a cytochrome P450 (CYP)-rich GA biosynthetic operon found in many symbiotic, nitrogen-fixing rhizobia led to the elucidation of bacterial GA biosynthesis and implicated GA9 as the final produ...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/acschembio.6b01038
更新日期：2017-04-21 00:00:00
abstract：:Chemical genetics is a powerful approach for identifying therapeutically active small molecules, but identifying the mechanisms of action underlying hit compounds remains challenging. Chemoproteomic platforms have arisen to tackle this challenge and enable rapid mechanistic deconvolution of small-molecule screening hi...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/acschembio.8b00381
更新日期：2018-08-17 00:00:00
abstract：:N-methyl-d-aspartate receptors (NMDARs) mediate glutamatergic signaling that is critical to cognitive processes in the central nervous system, and NMDAR hypofunction is thought to contribute to cognitive impairment observed in both schizophrenia and Alzheimer's disease. One approach to enhance the function of NMDAR is...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/acschembio.6b00913
更新日期：2017-02-17 00:00:00
abstract：:The canonical NF-κB signaling pathway is a mediator of the cellular inflammatory response and a target for developing therapeutics for multiple human diseases. The furthest downstream proteins in the pathway, the p50/p65 transcription factor heterodimer, have been recalcitrant toward small molecule inhibition despite ...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/acschembio.6b00751
更新日期：2017-01-20 00:00:00
abstract：:Tumor heterogeneity has hampered the development of novel effective therapeutic options for aggressive cancers, including the deadly primary adult brain tumor glioblastoma (GBM). Intratumoral heterogeneity is partially attributed to the tumor initiating cell (TIC) subset that contains highly tumorigenic, stem-like cel...
journal_title：ACS chemical biology
pub_type： 杂志文章
doi：10.1021/acschembio.8b00251
更新日期：2018-08-17 00:00:00
