Abstract：
Background:UP elements (upstream element) are DNA sequences upstream of a promoter that interact with the α-subunit of RNA polymerase (RNAP) and can affect transcription by altering the binding RNAP to DNA. However, details of UP element and binding affinity effects on transcriptional strength are unclear. Results:Here, we investigated the effects of UP element sequences on gene transcription, binding affinity, and gene expression noise. Addition of UP elements resulted in increased gene expression (maximum 95.7-fold increase) and reduced gene expression noise (8.51-fold reduction). Half UP element sequences at the proximal subsite has little effect on transcriptional strength despite increasing binding affinity by 2.28-fold. In vitro binding assays were used to determine dissociation constants (Kd) and in the in vitro system, the full range of gene expression occurs in a small range of dissociation constants (25 nM < Kd < 45 nM) indicating that transcriptional strength is highly sensitive to small changes in binding affinity. Conclusions:These results demonstrate the utility of UP elements and provide mechanistic insight into the functional relationship between binding affinity and transcription. Given the centrality of gene expression via transcription to biology, additional insight into transcriptional mechanisms can foster both fundamental and applied research. In particular, knowledge of the DNA sequence-specific effects on expression strength can aid in promoter engineering for different organisms and for metabolic engineering to balance pathway fluxes.
journal_name
J Biol Engjournal_title
Journal of biological engineeringauthors
Yan Q,Fong SSdoi
10.1186/s13036-017-0075-2subject
Has Abstractpub_date
2017-11-01 00:00:00pages
33issn
1754-1611pii
75journal_volume
11pub_type
杂志文章abstract：:Due to steadily growing population and economic transitions in the more populous countries, renewable sources of energy are needed more than ever. Plant biomass as a raw source of bioenergy and biofuel products may meet the demand for sustainable energy; however, such plants typically compete with food crops, which sh...
journal_title：Journal of biological engineering
pub_type： 杂志文章,评审
doi：10.1186/s13036-017-0069-0
更新日期：2017-09-01 00:00:00
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journal_title：Journal of biological engineering
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journal_title：Journal of biological engineering
pub_type： 杂志文章,评审
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pub_type： 杂志文章
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journal_title：Journal of biological engineering
pub_type： 杂志文章,评审
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journal_title：Journal of biological engineering
pub_type： 杂志文章
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更新日期：2014-07-01 00:00:00
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journal_title：Journal of biological engineering
pub_type： 杂志文章,评审
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journal_title：Journal of biological engineering
pub_type： 杂志文章,评审
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更新日期：2019-01-18 00:00:00
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pub_type： 杂志文章
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更新日期：2018-12-04 00:00:00
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pub_type： 杂志文章
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更新日期：2017-07-12 00:00:00
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journal_title：Journal of biological engineering
pub_type： 杂志文章
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更新日期：2020-01-13 00:00:00
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journal_title：Journal of biological engineering
pub_type： 杂志文章
doi：10.1186/1754-1611-6-18
更新日期：2012-09-14 00:00:00
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pub_type： 杂志文章
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journal_title：Journal of biological engineering
pub_type： 杂志文章
doi：10.1186/1754-1611-6-1
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pub_type： 杂志文章
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journal_title：Journal of biological engineering
pub_type： 杂志文章
doi：10.1186/s13036-017-0062-7
更新日期：2017-11-21 00:00:00
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journal_title：Journal of biological engineering
pub_type： 杂志文章
doi：10.1186/s13036-019-0143-x
更新日期：2019-02-07 00:00:00
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journal_title：Journal of biological engineering
pub_type： 杂志文章,评审
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pub_type： 杂志文章
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journal_title：Journal of biological engineering
pub_type： 杂志文章
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pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：Journal of biological engineering
pub_type： 杂志文章
doi：10.1186/1754-1611-7-26
更新日期：2013-11-18 00:00:00
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journal_title：Journal of biological engineering
pub_type： 杂志文章
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更新日期：2007-10-10 00:00:00
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journal_title：Journal of biological engineering
pub_type： 杂志文章
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更新日期：2013-12-02 00:00:00
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journal_title：Journal of biological engineering
pub_type： 杂志文章,评审
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更新日期：2020-09-09 00:00:00
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journal_title：Journal of biological engineering
pub_type： 杂志文章
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journal_title：Journal of biological engineering
pub_type： 杂志文章
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更新日期：2018-11-14 00:00:00
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journal_title：Journal of biological engineering
pub_type： 杂志文章
doi：10.1186/s13036-019-0187-y
更新日期：2019-06-28 00:00:00
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journal_title：Journal of biological engineering
pub_type： 杂志文章
doi：10.1186/1754-1611-2-10
更新日期：2008-07-09 00:00:00
