MicroRNA‑206 contributes to the progression of steroid‑induced avascular necrosis of the femoral head by inducing osteoblast apoptosis by suppressing programmed cell death 4.

Abstract:

:The expression of microRNA‑206 (miR‑206) is aberrantly induced in steroid‑induced avascular necrosis of femoral head (SANFH). Therefore, investigating the function of miR‑206 in SANFH and uncovering the functional mechanism associated with the condition will promote the understanding and treatment of the disease. The purpose of the present study was to investigate the pro‑osteoclasteogenic effect of miR‑206 that occurs through regulation of programmed cell death 4 (PDCD4). The expression of miR‑206 and PDCD4 was analyzed in the clinical SANFH specimens. The level of miR‑206 and PDCD4 was regulated in human osteoblast lineage hFOB1.19 and the effect of different treatments on cell viability, proliferation, apoptosis and differentiation potential of osteoblasts were analyzed with a Cell Counting kit‑8, 5‑ethynyl‑2'‑deoxyuridine staining, flow cytometry and Hoechst staining. The expression of miR‑206 was upregulated while PDCD4 was downregulated in the SANFH specimens. Induced expression of miR‑206 decreased cell viability and proliferation, while apoptosis was induced. At the molecular level, overexpression of miR‑206 inhibited the expression of PDCD4, alkaline phosphatase (ALP) and B‑cell lymphoma 2 (Bcl‑2), and increased the expression of apoptosis regulator Bcl2‑X‑associated protein (Bax). Inhibiting the expression of miR‑206 increased cell viability and proliferation but had no effect on cell apoptosis, as detected by flow cytometry and Hoechst staining. However, at the molecular level, inhibiting the expression of miR‑206 induced expression of PDCD4, ALP and Bcl‑2, while it decreased the expression of Bax. Additionally, knockdown of PDCD4 blocked the effect of miR‑206 inhibition on hFOB1.19 cells, representing a PDCD4‑dependent manner of miR‑206 in inducing apoptosis of osteoblasts. Therefore, miR‑206 promoted the onset of SANFH by inducing apoptosis and suppressed the proliferation of osteoblasts, which was dependent on the inhibition of PDCD4.

journal_name

Mol Med Rep

authors

Zhang Z,Jin A,Yan D

doi

10.3892/mmr.2017.7963

subject

Has Abstract

pub_date

2018-01-01 00:00:00

pages

801-808

issue

1

eissn

1791-2997

issn

1791-3004

journal_volume

17

pub_type

杂志文章
  • Increased burden of rare deleterious variants of the KCNQ1 gene in patients with large‑vessel ischemic stroke.

    abstract::The impact of rare and damaging variants in genes associated with platelet function in large‑vessel ischemic stroke (LVIS) remains unknown. The aim of this study was to investigate the contribution of some of these variants to the genetic susceptibility to LVIS in Polish patients using a deep re‑sequencing of 54 selec...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2019.9987

    authors: Janicki PK,Eyileten C,Ruiz-Velasco V,Pordzik J,Czlonkowska A,Kurkowska-Jastrzebska I,Sugino S,Imamura Kawasawa Y,Mirowska-Guzel D,Postula M

    更新日期:2019-04-01 00:00:00

  • Combined application of Rho-ROCKII and GSK-3β inhibitors exerts an improved protective effect on axonal regeneration in rats with spinal cord injury.

    abstract::Previous studies have reported that the Rho-associated coiled-coil containing protein kinase 2 (ROCKII) and glycogen synthase kinase‑3β (GSK)‑3β signaling pathways are involved in axonal regeneration. The present study investigated the effects of the combined application of Y27632 (a ROCKII inhibitor) and 4-benzyl-2‑m...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2016.5918

    authors: Zhang G,Lei F,Zhou Q,Feng D,Bai Y

    更新日期:2016-12-01 00:00:00

  • Combined CT angiography and SPECT myocardial perfusion imaging for the detection of functionally relevant coronary stenoses.

    abstract::The present study aimed to discuss and evaluate the diagnostic performances and incremental clinical values of combined computed tomography coronary angiography (CTCA) and single photon myocardial perfusion imaging (MPI) for the detection of functionally relevant coronary stenoses (FRCS). In total, 54 patients underwe...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2013.1354

    authors: Li JM,Shi RF,Zhang LR,Li T,Dong Z

    更新日期:2013-05-01 00:00:00

  • Exogenous activation of LKB1/AMPK signaling induces G₁ arrest in cells with endogenous LKB1 expression.

    abstract::The tumor suppressor protein LKB1 is a serine/threonine kinase that plays a critical role in cell proliferation, and its inactivation has been linked to tumorigenesis in various cancer types. Current understanding of the LKB1 function is largely restricted to results from experiments on LKB1‑deficient cancer cells, wh...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2014.1916

    authors: Liang X,Wang P,Gao Q,Tao X

    更新日期:2014-03-01 00:00:00

  • Molecular basis of degenerative spinal disorders from a proteomic perspective (Review).

    abstract::Intervertebral disc degeneration (IDD) and ligamentum flavum hypertrophy (LFH) are major causes of degenerative spinal disorders. Comparative and proteomic analysis was used to identify differentially expressed proteins (DEPs) in IDD and LFH discs compared with normal discs. Subsequent gene ontology term enrichment an...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2019.10812

    authors: Liu C,Yang M,Liu L,Zhang Y,Zhu Q,Huang C,Wang H,Zhang Y,Li H,Li C,Huang B,Feng C,Zhou Y

    更新日期:2020-01-01 00:00:00

  • The study on mechanism of the modified Chinese herbal compound, jianpijiedu, on a mouse model of hepatic carcinoma cachexia.

    abstract::Various studies have investigated hepatic carcinoma cachexia, however, there is little published information regarding the effect of Chinese Medicine carcinoma cachexia. The present study was performed to investigate the effect of modified Chinese herbal compound jianpijiedu (MJPJD) on a mouse model of ascites‑induced...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2016.5602

    authors: Sun B,Luo H,Deng L,Zhang S,Chen Z

    更新日期:2016-10-01 00:00:00

  • Silencing cyclin-dependent kinase inhibitor 3 inhibits the migration of breast cancer cell lines.

    abstract::Cyclin-dependent kinase inhibitor 3 (CDKN3) belongs to the dual-specificity protein phosphatase family, which is hypothesized to regulate cell cycle progression in tumor cells. However, whether CDKN3 is a potential therapeutic target for breast cancer remains to be elucidated. The present in vitro study aimed to inves...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2016.5401

    authors: Deng M,Wang J,Chen Y,Zhang L,Xie G,Liu Q,Zhang T,Yuan P,Liu D

    更新日期:2016-08-01 00:00:00

  • Resveratrol ameliorates low shear stress‑induced oxidative stress by suppressing ERK/eNOS‑Thr495 in endothelial cells.

    abstract::Fluid shear stress has been revealed to differentially regulate endothelial nitric oxide synthase (eNOS) distribution in vessels. eNOS, a key enzyme in controlling nitric oxide (NO) release, has a crucial role in mediating oxidative stress, and resveratrol (RSV)‑mediated eNOS also attenuates oxidative damage and suppr...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2014.2390

    authors: Wang Z,Zhang J,Li B,Gao X,Liu Y,Mao W,Chen SL

    更新日期:2014-10-01 00:00:00

  • Honokiol induces autophagy and apoptosis of osteosarcoma through PI3K/Akt/mTOR signaling pathway.

    abstract::Honokiol is the main active constituent of Magnolia officinalis. With effective and long‑term pharmacological functions of being antibacterial, anti‑oxidative, anti‑inflammatory, antitumor, anti‑spasmic, anti‑anxiety and anti‑viral, Honokiol is clinically used in the treatment of acute enteritis and chronic gastritis....

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2017.8123

    authors: Li Z,Dong H,Li M,Wu Y,Liu Y,Zhao Y,Chen X,Ma M

    更新日期:2018-02-01 00:00:00

  • Death receptor 5 expression is inversely correlated with prostate cancer progression.

    abstract::Prostate carcinoma (PCa) is one of the most common cancers in men. Prostate-specific antigen (PSA) has been widely used to predict the outcome of PCa and screening with PSA has resulted in a decline in mortality. However, PSA is not an optimal prognostic tool as its sensitivity may be too low to reduce morbidity and m...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2014.2504

    authors: Hernandez-Cueto A,Hernandez-Cueto D,Antonio-Andres G,Mendoza-Marin M,Jimenez-Gutierrez C,Sandoval-Mejia AL,Mora-Campos R,Gonzalez-Bonilla C,Vega MI,Bonavida B,Huerta-Yepez S

    更新日期:2014-11-01 00:00:00

  • Vascular endothelial growth factor induces anti‑Müllerian hormone receptor 2 overexpression in ovarian granulosa cells of in vitro fertilization/intracytoplasmic sperm injection patients.

    abstract::Misregulation of vascular endothelial growth factor A (VEGF‑A) has been implicated in numerous types of ovarian disease, such as polycystic ovarian syndrome, ovarian hyperstimulation syndrome, endometriosis and ovarian cancer. VEGF regulates blood vessel permeability and angiogenesis. In our previous study, VEGF‑regul...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2016.5173

    authors: Fang Y,Lu X,Liu L,Lin X,Sun M,Fu J,Xu S,Tan Y

    更新日期:2016-06-01 00:00:00

  • Carbon dioxide‑pneumoperitoneum in rats reduces ischemia/reperfusion‑induced hepatic apoptosis and inflammatory responses by stimulating sensory neurons.

    abstract::Laparoscopic surgery induces a milder inflammatory response than open surgery, however, the precise mechanisms underlying this phenomenon remain to be elucidated. Our previous study demonstrated that stimulation of sensory neurons inhibited hepatic apoptosis and inflammatory responses in rats subjected to hepatic isch...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2014.2329

    authors: Kawai M,Zhao J,Ishiguro H,Takeyama H

    更新日期:2014-09-01 00:00:00

  • C9orf100, a new member of the Dbl-family guanine nucleotide exchange factors, promotes cell proliferation and migration in hepatocellular carcinoma.

    abstract::Dbl-family guanine nucleotide exchange factors (GEFs) are important activators of Rho GTPases, which are significantly associated with tumorigenesis and metastasis. The catalytic ability of the Dbl-family GEFs to activate Rho GTPases depends on their Dbl-homology domain followed by a pleckstrin-homology domain. In the...

    journal_title:Molecular medicine reports

    pub_type: 临床试验,杂志文章

    doi:10.3892/mmr.2012.783

    authors: Wang H,Li Y,Wang Y,Han ZG,Cai B

    更新日期:2012-05-01 00:00:00

  • Betulinic acid prevents high glucose‑induced expression of extracellular matrix protein in cardiac fibroblasts by inhibiting the TGF‑β1/Smad signaling pathway.

    abstract::The proliferation and differentiation of cardiac fibroblasts (CFs) is central to cardiac fibrosis. Betulinic acid (BA) is an active compound isolated from the bark of the birch tree Betula spp. (Betulaceae) and has been shown to attenuate hepatic fibrosis. However, the effect of BA on the high glucose‑induced fibrosis...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2017.7323

    authors: Jiang L,Chen FX,Zang ST,Yang QF

    更新日期:2017-11-01 00:00:00

  • Diallyl trisulfide reverses drug resistance and lowers the ratio of CD133+ cells in conjunction with methotrexate in a human osteosarcoma drug-resistant cell subline.

    abstract::P-glycoprotein (P-gp) overexpression and tumor stem cells are thought to be important factors in the cross-resistance of cancer cells. There have been no studies on whether diallyl trisulfide (DATS) can reverse drug resistance in human osteosarcoma cells. In the present study, we demonstrated that DATS down-regulated ...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr_00000091

    authors: Li J,Liu W,Zhao K,Zhang Y,Li X,Yang Q,Li Z,Li J

    更新日期:2009-03-01 00:00:00

  • κ‑carrageenan‑derived pentasaccharide attenuates Aβ25‑35‑induced apoptosis in SH‑SY5Y cells via suppression of the JNK signaling pathway.

    abstract::β-amyloid (Aβ)-mediated neuronal apoptosis is an important pathological feature of Alzheimer's disease (AD). Inhibiting apoptosis induced by Aβ may lead to the development of a potential therapeutic target for AD treatment. κ‑carrageenan‑derived pentasaccharide (KCP) extracted from marine red algae is involved in a va...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2016.6006

    authors: Liu Y,Jiang L,Li X

    更新日期:2017-01-01 00:00:00

  • Grooved hydroxyapatite scaffold modulates mitochondria homeostasis and thus promotes osteogenesis in bone mesenchymal stromal cells.

    abstract::Hydroxyapatite scaffolds (HASs) are widely studied as suitable materials for bone replacement scaffolds due to their chemical similarities to organic materials. In our previous study, a novel HAS with a 25‑30‑µm groove structure (HAS‑G) exhibited enhanced osteogenesis of bone mesenchymal stromal cells (BMSCs) compared...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2020.11352

    authors: Li C,Yang L,Ren X,Lin M,Shen D,Li Y,Zhang X,Liu C,Mu Y

    更新日期:2020-10-01 00:00:00

  • Multinucleated cells are involved in normal development and apoptosis in mouse testes.

    abstract::Multinucleated cells are present in impaired spermatogenesis and in the senescent testis. Following accumulating evidence from our previous studies on the identification of multinucleated cells during normal testicle development, the current study further investigated the possible mechanism and role of these multinucl...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2013.1568

    authors: Luo L,Li Y,Yang Y,He Y,Wang Y,Xu Z,Zhang Y

    更新日期:2013-09-01 00:00:00

  • Differential long non‑coding RNA and mRNA expression in differentiated human glioblastoma stem cells.

    abstract::Differentiation of glioblastoma stem cells (GSCs) may lead to inhibition of their self‑renewing ability and tumorigenic potential, as well as increasing their sensitivity to treatment. The critical role of long non‑coding RNAs (lncRNAs) in numerous biological processes has been revealed. However, the involvement of ln...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2016.5505

    authors: Li H,Li H,Hao Y,Jiao Y,Li Z,Yue H,Xu Z,Wang S,Cao Y,Zhao J

    更新日期:2016-09-01 00:00:00

  • Effect of fractionated irradiation on the expression of multidrug resistance genes in the CNE1 human nasopharyngeal carcinoma cell line.

    abstract::Nasopharyngeal carcinoma (NPC) often develops drug resistance following radiotherapy. The molecular basis of radiotherapy-related multidrug resistance (MDR) remains unclear. In the present study, we investigated the effect of fractionated irradiation on the expression of the MDR-1 gene and the MDR-associated protein P...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2012.1148

    authors: Ji XN,Yang F,Sui XM,Wang FG,Ge RG,Quan XL,Zhao T,Gao BW,Wang RY

    更新日期:2013-01-01 00:00:00

  • Electrical stimulation by semi-implantable electrodes decreases the levels of proteins associated with sciatic nerve injury-induced muscle atrophy.

    abstract::Muscle atrophy is a disease that is usually caused by denervation. The aim of the present study was to determine whether electrical stimulation by semi-implantable electrodes is capable of decreasing the levels of specific proteins associated with sciatic nerve injury-induced muscle atrophy. Male Sprague Dawley (SD) r...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2013.1487

    authors: Liu J,Li K,Huang X,Xie J,Huang X

    更新日期:2013-07-01 00:00:00

  • The effect of mitotane on viability, steroidogenesis and gene expression in NCI‑H295R adrenocortical cells.

    abstract::Mitotane, also known as o,p'‑DDD or (RS)‑1‑chl-oro‑2‑[2,2‑dichloro‑1‑(4‑chlorophenyl)‑ethyl]‑benzene, is an adrenal cortex-specific cytotoxic drug used in the therapy of adrenocortical carcinoma (ACC). The drug also inhibits steroidogenesis, however, the mechanisms of its anticancer...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2012.1244

    authors: Lehmann TP,Wrzesiński T,Jagodziński PP

    更新日期:2013-03-01 00:00:00

  • Knockdown of lncRNA HNF1A-AS1 inhibits oncogenic phenotypes in colorectal carcinoma.

    abstract::Long non-coding RNAs (lncRNAs) have been demonstrated to serve important roles in the development and progression of cancer. Recently HNF1A antisense RNA 1 (HNF1A‑AS1), a lncRNA, has been reported as exhibiting a potential oncogenic role in the development of many types of cancer. However, the expression and the role ...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2017.7175

    authors: Zhu W,Zhuang P,Song W,Duan S,Xu Q,Peng M,Zhou J

    更新日期:2017-10-01 00:00:00

  • Overexpression of CCDC34 in colorectal cancer and its involvement in tumor growth, apoptosis and invasion.

    abstract::It has been previously reported that increased expression of coiled‑coil domain containing 34 (CCDC34), a member of the CCDCs family, may promote the proliferation and invasion of bladder cancer cells. However, its role in colorectal cancer (CRC) remains unclear. The present study investigated CCDC34 expression in CRC...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2017.7860

    authors: Geng W,Liang W,Fan Y,Ye Z,Zhang L

    更新日期:2018-01-01 00:00:00

  • MicroRNA-18a upregulates autophagy and ataxia telangiectasia mutated gene expression in HCT116 colon cancer cells.

    abstract::Autophagy is an evolutionarily conserved, multi-step lysosomal degradation process in which a cell degrades its own long-lived proteins and damaged organelles. Ataxia telangiectasia mutated (ATM) has recently been shown to upregulate the process of autophagy. Previous studies showed...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2012.1214

    authors: Qased AB,Yi H,Liang N,Ma S,Qiao S,Liu X

    更新日期:2013-02-01 00:00:00

  • Genistein decreases A549 cell viability via inhibition of the PI3K/AKT/HIF‑1α/VEGF and NF‑κB/COX‑2 signaling pathways.

    abstract::Genistein is an important chemopreventive agent against atherosclerosis and cancer. However, whether genistein is effective in the treatment of lung cancer, and its underlying mechanism, remains to be determined. The present study demonstrated that genistein treatment of A549 lung cancer cells decreased viability in a...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2017.6260

    authors: Zhang J,Su H,Li Q,Li J,Zhao Q

    更新日期:2017-04-01 00:00:00

  • Differentiation of human amniotic epithelial cells into osteoblasts is induced by mechanical stretch via the Wnt/β‑catenin signalling pathway.

    abstract::Human amniotic epithelial cells (hAECs) have recently been recognized as a potential source of stem cells. The present study was designed to investigate the effects of mechanical stretch on the osteogenic differentiation of hAECs. As it has been previously reported that the physical environment is an important factor ...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2018.9571

    authors: Luan F,Ma K,Mao J,Yang F,Zhang M,Luan H

    更新日期:2018-12-01 00:00:00

  • Chondrogenic differentiation of bone marrow‑derived stem cells cultured in the supernatant of elastic cartilage cells.

    abstract::Repair of cartilage defects remains a challenge for surgeons, owing to its poor self‑repairing capacity. Cartilage tissue engineering, particularly marrow stem cell‑based cartilage regeneration, provides a promising option for the regeneration of damaged cartilage. Although producing tissue‑engineered cartilage from m...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2015.4113

    authors: Zhang X,Xue K,Zhou J,Xu P,Huang H,Liu K

    更新日期:2015-10-01 00:00:00

  • Reduced expression of SIRT2 in serous ovarian carcinoma promotes cell proliferation through disinhibition of CDK4 expression.

    abstract::The silent information regulator 2 related enzyme 2 (SIRT2) has been reported to have an important role in tumorigenesis. Although two distinct effects of SIRT2 have recently been revealed, which explain opposing expression patterns in different types of cancer, the specific function of SIRT2 in ovarian cancer remains...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2017.6183

    authors: Du Y,Wu J,Zhang H,Li S,Sun H

    更新日期:2017-04-01 00:00:00

  • Hypoxia in utero increases the risk of pulmonary hypertension in rat offspring and is associated with vasopressin type‑2 receptor upregulation.

    abstract::Pulmonary hypertension (PH) in newborns and adults is a disease that can lead to right heart failure and result in a shorter lifespan. PH was induced by maintaining pregnant rats in a hypoxic chamber for 4 h twice a day, from days 7‑21 of pregnancy. Hypoxia was confirmed by a decrease in the partial pressure of oxygen...

    journal_title:Molecular medicine reports

    pub_type: 杂志文章

    doi:10.3892/mmr.2020.11533

    authors: Ding H,Luo Y,Hu K,Huang H,Liu P,Xiong M,Zhu L,Yi J,Xu Y

    更新日期:2020-11-01 00:00:00