Abstract:
:Pompe disease, a rare lysosomal storage disease caused by deficiency of the lysosomal acid α-glucosidase (GAA), is characterized by glycogen accumulation, triggering severe secondary cellular damage and resulting in progressive motor handicap and premature death. Numerous disease-causing mutations in the gaa gene have been reported, but the structural effects of the pathological variants were unknown. Here we present the high-resolution crystal structures of recombinant human GAA (rhGAA), the standard care of Pompe disease. These structures portray the unbound form of rhGAA and complexes thereof with active site-directed inhibitors, providing insight into substrate recognition and the molecular framework for the rationalization of the deleterious effects of disease-causing mutations. Furthermore, we report the structure of rhGAA in complex with the allosteric pharmacological chaperone N-acetylcysteine, which reveals the stabilizing function of this chaperone at the structural level.
journal_name
Nat Communjournal_title
Nature communicationsauthors
Roig-Zamboni V,Cobucci-Ponzano B,Iacono R,Ferrara MC,Germany S,Bourne Y,Parenti G,Moracci M,Sulzenbacher Gdoi
10.1038/s41467-017-01263-3subject
Has Abstractpub_date
2017-10-24 00:00:00pages
1111issue
1issn
2041-1723pii
10.1038/s41467-017-01263-3journal_volume
8pub_type
杂志文章abstract::Nanoscale coordination polymers (NCPs) are self-assembled from metal ions and organic bridging ligands, and can overcome many drawbacks of existing drug delivery systems by virtue of tunable compositions, sizes and shapes, high drug loadings, ease of surface modification and intrinsic biodegradability. Here we report ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms5182
更新日期:2014-06-25 00:00:00
abstract::The general paradigm is that monocytes are recruited to sites of inflammation and terminally differentiate into macrophages. There has been no demonstration of proliferation of peripherally-derived inflammatory macrophages under physiological conditions. Here we show that proliferation of both bone marrow-derived infl...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms2877
更新日期:2013-01-01 00:00:00
abstract::Lifespan is a trait of enormous personal interest. Research into the biological basis of human lifespan, however, is hampered by the long time to death. Using a novel approach of regressing (272,081) parental lifespans beyond age 40 years on participant genotype in a new large data set (UK Biobank), we here show that ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms11174
更新日期:2016-03-31 00:00:00
abstract::Artemisinin and its derivatives (collectively referred to as ARTs) rapidly reduce the parasite burden in Plasmodium falciparum infections, and antimalarial control is highly dependent on ART combination therapies (ACTs). Decreased sensitivity to ARTs is emerging, making it critically important to understand the mechan...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-06221-1
更新日期:2018-09-18 00:00:00
abstract::Magnetic skyrmions are localized, topologically protected spin structures that have been proposed for storing or processing information due to their intriguing dynamical and transport properties. Important in terms of applications is the recent discovery of interface stabilized skyrmions as evidenced in ultra-thin tra...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms11779
更新日期:2016-06-03 00:00:00
abstract::Bacterial CRISPR-Cas systems comprise diverse effector endonucleases with different targeting ranges, specificities and enzymatic properties, but many of them are inactive in mammalian cells and are thus precluded from genome-editing applications. Here we show that the type II-B FnCas9 from Francisella novicida posses...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms14958
更新日期:2017-04-07 00:00:00
abstract::Microorganisms form biofilms containing differentiated cell populations. To determine factors driving differentiation, we herein visualize protein and metal distributions within Pseudomonas aeruginosa biofilms using imaging mass spectrometry. These in vitro experiments reveal correlations between differential protein ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms11951
更新日期:2016-06-15 00:00:00
abstract::Metal oxides with a tunnelled structure are attractive as charge storage materials for rechargeable batteries and supercapacitors, since the tunnels enable fast reversible insertion/extraction of charge carriers (for example, lithium ions). Common synthesis methods can introduce large cations such as potassium, barium...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms13374
更新日期:2016-11-21 00:00:00
abstract::The photon-like behavior of electrons in graphene causes unusual confinement properties that depend strongly on the geometry and strength of the surrounding potential. We report bottom-up synthesis of atomically-precise one-dimensional (1D) arrays of point charges on graphene that allow exploration of a new type of su...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-08371-2
更新日期:2019-01-29 00:00:00
abstract::Sign epistasis is a central evolutionary constraint, but its causal factors remain difficult to predict. Here we use the notion of parameterised optima to explain epistasis within a signalling cascade, and test these predictions in Escherichia coli. We show that sign epistasis arises from the benefit of tuning phenoty...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-03644-8
更新日期:2018-04-13 00:00:00
abstract::Bright solitons are non-dispersive wave solutions, arising in a diverse range of nonlinear, one-dimensional systems, including atomic Bose-Einstein condensates with attractive interactions. In reality, cold-atom experiments can only approach the idealized one-dimensional limit necessary for the realization of true sol...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms2893
更新日期:2013-01-01 00:00:00
abstract::Recent discovery of the half quantized thermal Hall conductivity in [Formula: see text]-RuCl[Formula: see text], a candidate material for the Kitaev spin liquid, suggests the presence of a highly entangled quantum state in external magnetic fields. This field induced phase appears between the low field zig-zag magneti...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-15320-x
更新日期:2020-04-02 00:00:00
abstract::Van Allen radiation belts are typically two zones of energetic particles encircling the Earth separated by the slot region. How the outer radiation belt electrons are accelerated to relativistic energies remains an unanswered question. Recent studies have presented compelling evidence for the local acceleration by ver...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms10096
更新日期:2015-12-22 00:00:00
abstract::Previous studies have revealed that nucleosomes impede elongation of RNA polymerase II (RNAPII). Recent observations suggest a role for ATP-dependent chromatin remodellers in modulating this process, but direct in vivo evidence for this is unknown. Here using fission yeast, we identify Fun30Fft3 as a chromatin remodel...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms14527
更新日期:2017-02-20 00:00:00
abstract::Chiral metal complexes show promise as asymmetric catalysts and optical materials. Chiral-at-metal complexes composed of achiral ligands have expanded the versatility and applicability of chiral metal complexes, especially for octahedral and half-sandwich complexes. However, Werner-type tetrahedral complexes with a st...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-20074-7
更新日期:2020-12-09 00:00:00
abstract::Hollow-core fibre (HCF) is a powerful technology platform offering breakthrough performance improvements in sensing, communications, higher-power pulse delivery and other applications. Free from the usual constraints on what materials can guide light, it promises qualitatively new and ideal operating regimes: precisio...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms6085
更新日期:2014-10-28 00:00:00
abstract::The original version of this article contained an error in the spelling of the author Christian A.M. Wilson, which was incorrectly given as Christian M.A. Wilson. This has now been corrected in both the PDF and HTML versions of the article. ...
journal_title:Nature communications
pub_type: 杂志文章,已发布勘误
doi:10.1038/s41467-017-02384-5
更新日期:2017-12-14 00:00:00
abstract::Individual cells in clonal populations often respond differently to environmental changes; for binary phenotypes, such as cell death, this can be measured as a fractional response. These types of responses have been attributed to cell-intrinsic stochastic processes and variable abundances of biochemical constituents, ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-09275-x
更新日期:2019-03-21 00:00:00
abstract::Inelastic collisions involving molecular species are key to energy transfer in gaseous environments. They are commonly governed by an energy gap law, which dictates that transitions are dominated by those between initial and final states with roughly the same ro-vibrational energy. Transitions involving rotational ine...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-12691-8
更新日期:2019-10-11 00:00:00
abstract::Acid-sensing ion channel 3 (ASIC3) is involved in acid nociception, but its possible role in neurosensory mechanotransduction is disputed. We report here the generation of Asic3-knockout/eGFPf-knockin mice and subsequent characterization of heterogeneous expression of ASIC3 in the dorsal root ganglion (DRG). ASIC3 is ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms11460
更新日期:2016-05-10 00:00:00
abstract::Interfacial issues commonly exist in solid-state batteries, and the microstructural complexity combines with the chemical heterogeneity to govern the local interfacial chemistry. The conventional wisdom suggests that "point-to-point" ion diffusion at the interface determines the ion transport kinetics. Here, we show t...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-19528-9
更新日期:2020-11-11 00:00:00
abstract::In 1816, the coldest summer of the past two centuries was observed over northeastern North America and western Europe. This so-called Year Without a Summer (YWAS) has been widely attributed to the 1815 eruption of Indonesia's Mt. Tambora and was concurrent with agricultural failures and famines worldwide. To understan...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-017-01302-z
更新日期:2017-10-31 00:00:00
abstract::Human immunodeficiency virus (HIV) pre-exposure prophylaxis (PrEP) strategies with proven in vivo efficacy rely on antiretroviral drugs, creating the potential for drug resistance and complicated treatment options in individuals who become infected. Moreover, on-demand products are currently missing from the PrEP deve...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-06349-0
更新日期:2018-09-24 00:00:00
abstract::It is unclear whether the establishment of apical-basal cell polarity during the generation of epithelial lumens requires molecules acting at the plasma membrane/actin interface. Here, we show that the I-BAR-containing IRSp53 protein controls lumen formation and the positioning of the polarity determinants aPKC and po...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-17091-x
更新日期:2020-07-14 00:00:00
abstract::Flicker or 1/f noise in metal-oxide-semiconductor field-effect transistors (MOSFETs) has been identified as the main source of noise at low frequency. It often originates from an ensemble of a huge number of charges becoming trapped and de-trapped. However, as a deviation from the well-known model of 1/f noise is obse...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms1092
更新日期:2010-10-19 00:00:00
abstract::Scleroderma is an autoimmune rheumatic disorder accompanied by severe fibrosis in skin and other internal organs. During scleroderma progression, resident fibroblasts undergo activation and convert to α-smooth muscle actin (α-SMA) expressing myofibroblasts (MFBs) with increased capacity to synthesize collagens and fib...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-09101-4
更新日期:2019-03-08 00:00:00
abstract::Multipotent mesenchymal stromal cells (MSCs) are required for skeletal formation, maintenance, and repair throughout life; however, current models posit that postnatally arising long-lived adult MSCs replace transient embryonic progenitor populations. We previously reported exclusive expression and function of the emb...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-11100-4
更新日期:2019-07-18 00:00:00
abstract::Despite progress in understanding pollination network structure, the functional roles of floral sensory stimuli (visual, olfactory) have never been addressed comprehensively in a community context, even though such traits are known to mediate plant-pollinator interactions. Here, we use a comprehensive dataset of flora...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-03448-w
更新日期:2018-03-12 00:00:00
abstract::The three-dimensional architecture of the genome affects genomic functions. Multiple genome architectures at different length scales, including chromatin loops, domains, compartments, and lamina- and nucleolus-associated regions, have been discovered. However, how these structures are arranged in the same cell and how...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-16732-5
更新日期:2020-06-09 00:00:00
abstract::The 26S proteasome is the primary machinery that degrades ubiquitin (Ub)-conjugated proteins, including many proteotoxic proteins implicated in neurodegeneraton. It has been suggested that the elevation of proteasomal activity is tolerable to cells and may be beneficial to prevent the accumulation of protein aggregate...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms6633
更新日期:2014-12-05 00:00:00