NMDA receptor dependent changes in c-fos and p-CREB signaling following extinction and reinstatement of morphine place preference.

Abstract:

:Neural circuitry comprising the ventral tegmental area, nucleus accumbens (NAc), prefrontal cortex (PFC) and hippocampus (HIP) has a main role in reward phenomena. Previous behavioral studies indicated that intracerebroventricular administration of AP5 (NMDA glutamate receptor antagonist) and CNQX (AMPA/kainate glutamate receptor antagonist) during the extinction and before reinstatement of morphine-induced conditioned place preference (CPP) reduced the extinction period and reinstatement of morphine-CPP. Therefore, in the present study, we tried to evaluate the effect of antagonism of NMDA glutamate receptors on the p-CREB/CREB ratio and c-fos expression in the NAc, PFC and HIP during these two phases of morphine-CPP in male adult albino Wistar rats. The p-CREB/CREB ratio and c-fos levels were estimated by Western blot analysis. The results revealed that these two factors decreased by antagonism of NMDA glutamate receptors (different doses of AP5) compared to saline-control group in aforementioned regions. The reduction of molecular markers, especially the p-CREB/CREB ratio, after AP5 administration was more during the extinction period. Therefore, it can be assumed that consolidation and reconsolidation of morphine memory via intra-PFC, -NAc and -HIP NMDA glutamate receptors are in accordance with changes in p-CREB/CREB ratio and c-fos levels.

journal_name

Neurosci Lett

journal_title

Neuroscience letters

authors

Siahposht-Khachaki A,Ezzatpanah S,Razavi Y,Haghparast A

doi

10.1016/j.neulet.2017.10.028

subject

Has Abstract

pub_date

2018-01-01 00:00:00

pages

147-151

eissn

0304-3940

issn

1872-7972

pii

S0304-3940(17)30854-6

journal_volume

662

pub_type

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