Control of chrysanthemum flowering through integration with an aging pathway.

Abstract:

:Age, as a threshold of floral competence acquisition, prevents precocious flowering when there is insufficient biomass, and ensures flowering independent of environmental conditions; however, the underlying regulatory mechanisms are largely unknown. In this study, silencing the expression of a nuclear factor gene, CmNF-YB8, from the short day plant chrysanthemum (Chrysanthemum morifolium), results in precocious transition from juvenile to adult, as well as early flowering, regardless of day length conditions. The expression of SQUAMOSA PROMOTER BINDING-LIKE (SPL) family members, SPL3, SPL5, and SPL9, is upregulated in CmNF-YB8-RNAi plants, while expression of the microRNA, cmo-MIR156, is downregulated. In addition, CmNF-YB8 is shown to bind to the promoter of the cmo-MIR156 gene. Ectopic expression of cmo-miR156, using a virus-based microRNA expression system, restores the early flowering phenotype caused by CmNF-YB8 silencing. These results show that CmNF-YB8 influences flowering time through directly regulating the expression of cmo-MIR156 in the aging pathway.The mechanisms by which plant age regulates flowering remain incompletely understood. Here the authors show that age dependent regulation of SPL transcription factors by miR156 influence flowering via control of NF-YB8 expression in Chrysanthemum.

journal_name

Nat Commun

journal_title

Nature communications

authors

Wei Q,Ma C,Xu Y,Wang T,Chen Y,Lü J,Zhang L,Jiang CZ,Hong B,Gao J

doi

10.1038/s41467-017-00812-0

subject

Has Abstract

pub_date

2017-10-10 00:00:00

pages

829

issue

1

issn

2041-1723

pii

10.1038/s41467-017-00812-0

journal_volume

8

pub_type

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