Abstract:
:Accumulating evidences suggest that p53 is a key coordinator of cellular events triggered by oxidative stress often associated with the impairment in thiamine metabolism and its functions. However, there are limited data regarding the pursuant feedback between p53 transactivation and thiamine homeostasis. Impairment in thiamine metabolism can be induced experimentally via interference with the thiamine uptake and/or inhibition of the thiamin pyrophosphate-dependent enzymes using thiamine antagonists - amprolium (AM), oxythiamine (OT) or pyrithiamine (PT). We found that exposure of neuronally differentiated SH-SY5Y cells to AM, OT and PT triggered upregulation of p53 gene expression, post-translational modification of p53 via phosphorylation and activation of p53 DNA-binding activity. Phosphorylation of p53 at Ser20 was equally efficient in upregulation of thiamine transporter 1 (THTR1) by all antagonists. However, induction of the expressions of the pyruvate dehydrogenase E1 component subunit beta (PDHB) and oxoglutarate dehydrogenase (OGDH) required dual phosphorylation of p53 at Ser9 and Ser20, seen in cells treated with PT and OT. Moreover, pretreatment of the cells with a decoy oligonucleotide carrying wild-type p53-response element markedly attenuated OT-induced THTR1, PDHB and OGDH gene expression suggesting an important role of p53 in transactivation of these genes. Finally, analysis of gene and metabolic networks showed that OT triggers cell apoptosis through the p53-dependent intrinsic pathway.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Chornyy S,Parkhomenko Y,Chorna Ndoi
10.1038/s41598-017-10878-xsubject
Has Abstractpub_date
2017-09-06 00:00:00pages
10632issue
1issn
2045-2322pii
10.1038/s41598-017-10878-xjournal_volume
7pub_type
杂志文章abstract::A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper. ...
journal_title:Scientific reports
pub_type: 已发布勘误
doi:10.1038/s41598-018-21981-y
更新日期:2018-03-06 00:00:00
abstract::The estrogen signaling pathway has been reported to modulate prostate cancer (PCa) progression through the activity of estrogen receptors α and β (ERα and ERβ). Given that selective estrogen receptor modulators (SERMs) are used to treat breast cancer, ERs have been proposed as attractive therapeutic targets in PCa. Ho...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-60844-3
更新日期:2020-03-04 00:00:00
abstract::Drug resistance caused by excessive and indiscriminate antibiotic usage has become a serious public health problem. The need of finding new antibacterial drugs is more urgent than ever before. Tyrosyl-tRNA synthase was proved to be a potent target in combating drug-resistant bacteria. In silico methodologies including...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-10618-1
更新日期:2017-09-14 00:00:00
abstract::TP53 mutation (i.e., loss of normal-p53) may evoke epithelial-mesenchymal transition (EMT), which was previously attributed to loss of certain miRNAs. However, not all epithelial cells undergo EMT upon TP53 mutation, and the p53-miRNA axis may not fully explain p53 function in epithelial integrity. We here show two mo...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-20043-7
更新日期:2018-01-25 00:00:00
abstract::Bacterial blight and bacterial leaf streak are serious, economically damaging, diseases of rice caused by the bacteria Xanthomonas oryzae pv. oryzae and X. oryzae pv. oryzicola. Bacillus amyloliquefaciens FZB42 was shown to possess biocontrol activity against these Xanthomonas strains by producing the antibiotic compo...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep12975
更新日期:2015-08-13 00:00:00
abstract::Period circadian clock (Per) genes Per1 and Per2 have essential roles in circadian oscillation. In this study, we identified a new role of Per1-Per2 cooperation, and its mechanism, using our new experimental methods. Under constant light conditions, the period length of Per1 and Per2 knockout mice depended on the copy...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep32769
更新日期:2016-09-09 00:00:00
abstract::Spatially-resolved neutron powder diffraction with a gauge volume of 2 × 2 × 20 mm(3) has been applied as an in situ method to probe the lithium concentration in the graphite anode of different Li-ion cells of 18650-type in charged state. Structural studies performed in combination with electrochemical measurements an...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep18380
更新日期:2015-12-18 00:00:00
abstract::Pluripotent cells can be subdivided into two distinct states, the naïve and the primed state, the latter being further advanced on the path of differentiation. There are substantial differences in the regulation of pluripotency between human and mouse, and in humans only stem cells that resemble the primed state in mo...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep07927
更新日期:2015-01-21 00:00:00
abstract::Adaptation of visual responses enhances visual information processing mainly by preserving the full dynamic range of neuronal responses during changing light conditions and is found throughout the whole visual system. Although adaptation in the primate superior colliculus neurons has received much attention little is ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-27331-2
更新日期:2018-06-12 00:00:00
abstract::Laser speckle flowmetry suffers from a debated quantification of the inverse relation between decorrelation time (τc) and blood flow velocity (V), i.e. 1/τc = αV. Using a modified microcirculation imager (integrated sidestream dark field - laser speckle contrast imaging [SDF-LSCI]), we experimentally investigate on th...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep25258
更新日期:2016-04-29 00:00:00
abstract::To investigate changes in the size of the population of patients who are receiving long-term, active treatment for neovascular age-related macular degeneration (AMD). This retrospective, observational study included 18,165 patients who received anti-vascular endothelial growth factor injections (3,974 eyes). The injec...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-49749-y
更新日期:2019-09-13 00:00:00
abstract::Rapid integration of high-quality functional devices in microchannels is in highly demand for miniature lab-on-a-chip applications. This paper demonstrates the embellishment of existing microfluidic devices with integrated micropatterns via femtosecond laser MRAF-based holographic patterning (MHP) microfabrication, wh...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep33281
更新日期:2016-09-13 00:00:00
abstract::The Synlestidae (Odonata: Zygoptera) of southern Africa comprise some highly localized species. All but one species are endemic to South Africa, and many to the Cape Floristic Region. Here we present the first phylogenetic reconstruction of the southern African Synlestidae using nuclear and mitochondrial molecular dat...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-72001-x
更新日期:2020-09-15 00:00:00
abstract::Three main surveillance systems (laboratory-confirmed, influenza-like illness (ILI) and nationwide Notifiable Infectious Diseases Reporting Information System (NIDRIS)) have been used for influenza surveillance in China. However, it is unclear which surveillance system is more reliable in developing influenza early wa...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-21059-9
更新日期:2018-02-12 00:00:00
abstract::Male infertility is a worldwide medical problem. Asthenozoospermia is a common cause of infertility. Epigenetic modifications of DNA and histones have been shown to influence human infertility, but no research has explored whether N(6)-methyladenosine (m(6)A) level in RNA is associated with asthenozoospermia. Here, we...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep24345
更新日期:2016-04-13 00:00:00
abstract::Greenhouse gas (GHG) emissions from livestock grazing systems are contributing to global warming. To examine the influence of yak grazing systems on GHG fluxes and relationships between GHG fluxes and environmental factors, we measured carbon dioxide (CO2), methane (CH4) and nitrous oxide (N2O) fluxes over three key s...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep40857
更新日期:2017-01-20 00:00:00
abstract::DNA double-strand break (DSB) repair is crucial to maintain genomic stability. The fidelity of the repair depends on the complexity of the lesion, with clustered DSBs being more difficult to repair than isolated breaks. Using live cell imaging of heavy ion tracks produced at a high-energy particle accelerator we visua...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-58084-6
更新日期:2020-01-29 00:00:00
abstract::The Morris Water Maze is commonly used in behavioural neuroscience for the study of spatial learning with rodents. Over the years, various methods of analysing rodent data collected during this task have been proposed. These methods span from classical performance measurements to more sophisticated categorisation tech...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-33456-1
更新日期:2018-10-10 00:00:00
abstract::Prostate cancer (PCa) is the most common solid neoplasm diagnosed in developed countries. Nerve-sparing radical prostatectomy (NS-RP) has been widely accepted as the best choice treatment for localised PCa. However, erectile dysfunction (ED) and urinary incontinence are commonly observed after NS-RP. Using meta-analys...
journal_title:Scientific reports
pub_type: 杂志文章,评审
doi:10.1038/srep05801
更新日期:2014-07-23 00:00:00
abstract::With the development of ultra-intense laser technology, MeV ions can be obtained from laser-foil interactions in the laboratory. These energetic ion beams can be applied in fast ignition for inertial confinement fusion, medical therapy, and proton imaging. However, these ions are mainly accelerated in the laser propag...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep08274
更新日期:2015-02-05 00:00:00
abstract::Tunicates are marine invertebrates that compose the closest phylogenetic group to the vertebrates. These chordates present a particularly diverse range of regenerative abilities and life-history strategies. Consequently, tunicates provide an extraordinary perspective into the emergence and diversity of these traits. H...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-23749-w
更新日期:2018-04-03 00:00:00
abstract::Graphene's unique physical and chemical properties make it an attractive platform for use in micro- and nanoelectronic devices. However, electrostatically controlling the flow of electrons in graphene can be challenging as a result of Klein tunneling, where electrons normally incident to a one-dimensional potential ba...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep08435
更新日期:2015-02-13 00:00:00
abstract::Excessive Zn2+ causes brain damage via promoting ROS generation. Here we investigated the role of ROS-sensitive TRPM2 channel in H2O2/Zn2+-induced Ca2+ signalling and cell death in microglial cells. H2O2/Zn2+ induced concentration-dependent increases in cytosolic Ca2+ concentration ([Ca2+]c), which was inhibited by PJ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep45032
更新日期:2017-03-21 00:00:00
abstract::Deprivation of maternal care during early development markedly affects emotional development, but the underlying neuromolecular mechanisms are not fully understood. In a mouse model of disrupted mother-infant relationship, early weaning causes long-term impacts on pups to exhibit increased corticosterone secretion, an...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-40530-9
更新日期:2019-03-08 00:00:00
abstract::Nutrient resorption processes in the plants infected by pathogen remain poorly understood. Huanglongbing (HLB) is a destructive disease of citrus. HLB-pathogen 'Candidatus Liberibacter asiaticus' grows specifically in the phloem of hosts and may cause problems in the plant vascular system after infection. Therefore, i...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep14675
更新日期:2015-09-30 00:00:00
abstract::This study aimed to investigate changes in types of dental arch form during adolescence and explore adolescent changes in size and form of dental arch. Hong Kong Chinese were recruited and digital dental arch models were obtained at ages 12, 15, and 18 years. Geometric morphometrics was used to investigate adolescent ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-55073-2
更新日期:2019-12-06 00:00:00
abstract::Heterogeneous catalysis, which is widely used in the chemical industry, makes a great use of supported late-transition-metal nanoparticles, and bimetallic catalysts often show superior catalytic performances as compared to their single metal counterparts. In order to optimize catalyst efficiency and discover new activ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep35226
更新日期:2016-10-14 00:00:00
abstract::Increasing population has posed insurmountable challenges to agriculture in the provision of future food security, particularly in the Middle East and North Africa (MENA) region where biophysical conditions are not well-suited for agriculture. Iran, as a major agricultural country in the MENA region, has long been in ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-08066-y
更新日期:2017-08-09 00:00:00
abstract::Stable isotopes in water (e.g., δ2H and δ18O) are important indicators of hydrological and ecological patterns and processes. Tap water can reflect integrated features of regional hydrological processes and human activities. China is a large country with significant meteorological and geographical variations. This rep...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep43653
更新日期:2017-03-02 00:00:00
abstract::We compared the effects of 12-week programs of resistance training (RT), high-intensity interval aerobic training (HIAT), and moderate-intensity continuous aerobic training (MICT). The primary goal was to evaluate the therapeutic effects of the exercise modalities for the management of nonalcoholic fatty liver disease...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep43029
更新日期:2017-02-22 00:00:00