Abstract：
:Human lactoferrin (hLF) is a valuable protein for pharmaceutical products and functional foods, and worldwide demand for this protein has steadily increased. However, large-scale recombinant human lactoferrin (rhLF) production using current animal bioreactor techniques is limited by the low expression of foreign proteins, the use of antibiotic resistance genes and the down-regulation of endogenous milk proteins. Here, we generated a herd of marker-free, hLF bacterial artificial chromosome (BAC) transgenic cloned cows, as confirmed by Polymerase chain reaction, Southern blot and Western blot analyses. These transgenic cloned cows produced rhLF in milk at concentrations of 4.5-13.6 g/L. Moreover, the total protein content of the milk was increased. Over two hundred transgenic cloned cows were propagated by multiple ovulation and embryo transfer (MOET). A total of 400-450 g of rhLF protein, which shows similar enzymatic activity to natural hLF in iron binding and release, can be purified on a large scale from >100 L of milk per day. Our results suggested that transgenic bovine mammary bioreactors have the potential for large-scale protein production.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Wang M,Sun Z,Yu T,Ding F,Li L,Wang X,Fu M,Wang H,Huang J,Li N,Dai Ydoi
10.1038/s41598-017-11462-zsubject
Has Abstractpub_date
2017-09-06 00:00:00pages
10733issue
1issn
2045-2322pii
10.1038/s41598-017-11462-zjournal_volume
7pub_type
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