当前位置： SCI文献检索 > Asian Pacific Journal of Tropical Medicine期刊下所有文献 > First report on molecular characterization of Leishmania species from cutaneous leishmaniasis patients in southern Khyber Pakhtunkhwa province of Pakistan.

First report on molecular characterization of Leishmania species from cutaneous leishmaniasis patients in southern Khyber Pakhtunkhwa province of Pakistan.

Abstract：

OBJECTIVE:To report presence of Leishmania major in Khyber Pakhtunkhwa of Pakistan, where cutaneous leishmaniasis (CL) is endemic and was thought to be caused by Leishmania tropica only. METHODS:Biopsy samples from 432 CL suspected patients were collected from 3 southern districts of Khyber Pakhtunkhwa during years 2011-2016. Microscopy on Giemsa stained slides were done followed by amplification of the ribosomal internal transcribed spacer 1 gene. RESULTS:Leishmania amastigotes were detected by microscopy in 308 of 432 samples (71.3%) while 374 out of 432 samples (86.6%) were positive by ribosomal internal transcribed spacer 1 PCR. Subsequent restriction fragment length polymorphism confirmed L. tropica in 351 and L. major in 6 biopsy samples. CONCLUSIONS:This study is the first molecular characterization of Leishmania species in southern Khyber Pakhtunkhwa. It confirmed the previous assumptions that anthroponotic CL is the major CL form present in Khyber Pakhtunkhwa province. Furthermore, this is the first report of L. major from a classical anthroponotic CL endemic focus identified in rural areas of Kohat district in southern Khyber Pakhtunkhwa.

journal_name

Asian Pac J Trop Med

journal_title

Asian Pacific journal of tropical medicine

authors

Hussain M,Munir S,Ayaz S,Khattak BU,Khan TA,Muhammad N,Anees M,Rahman H,Qasim M,Jamal MA,Ahmed I,Rahim K,Mazhar H,Watanay N,Kasbari M

doi

10.1016/j.apjtm.2017.07.015

subject

Has Abstract

pub_date

2017-07-01 00:00:00

pages

718-721

issue

7

eissn

1995-7645

issn

2352-4146

pii

S1995-7645(17)30144-X

journal_volume

10

pub_type

杂志文章

相关文献

Asian Pacific Journal of Tropical Medicine文献大全
  • Tissue tropism and parasite burden of Toxoplasma gondii RH strain in experimentally infected mice.

    abstract：OBJECTIVE:To evaluate parasite distribution and tissue tropism of Toxoplasma gondii tachyzoites in experimentally infected mice using real time QPCR. METHODS:In this survey 16 Balb/c mice were inoculated with 1 × 10(4) alive tachyzoites of Toxoplasma gondii RH strain. After 1, 2, 3 days post infection and the last day...

    journal_title：Asian Pacific journal of tropical medicine

    pub_type： 杂志文章

    doi：10.1016/S1995-7645(14)60087-0

    authors： Dadimoghaddam Y,Daryani A,Sharif M,Ahmadpour E,Hossienikhah Z

    更新日期：2014-07-01 00:00:00

  • Malaria helminth co-infections and their contribution for aneamia in febrile patients attending Azzezo health center, Gondar, Northwest Ethiopia: a cross sectional study.

    abstract：OBJECTIVE:To assess the prevalence of malaria helminth co-infections and their contribution for aneamia in febrile patients attending Azzezo health center, Gondar, Northwest Ethiopia. METHODS:A cross section study was conducted among febrile patients attending Azezo health center from February-March 30, 2011. Convenie...

    journal_title：Asian Pacific journal of tropical medicine

    pub_type： 杂志文章

    doi：10.1016/S1995-7645(12)60147-3

    authors： Alemu A,Shiferaw Y,Ambachew A,Hamid H

    更新日期：2012-10-01 00:00:00

  • Isolation and characterization of anti-diabetic component (bioactivity-guided fractionation) from Ocimum sanctum L. (Lamiaceae) aerial part.

    abstract：OBJECTIVE:To isolate and characterize antidiabetic component (bioactivity-guided fractionation) from hydro alcoholic extract of Ocimum sanctum (O. sanctum) aerial part. METHODS:Ten fractions (F1-F10) were isolated from hydro alcoholic extract of O. sanctum aerial part by column chromatography. All the fractions F1 to ...

    journal_title：Asian Pacific journal of tropical medicine

    pub_type： 杂志文章

    doi：10.1016/S1995-7645(11)60086-2

    authors： Patil R,Patil R,Ahirwar B,Ahirwar D

    更新日期：2011-04-01 00:00:00

  • Escherichia coli tetracycline efflux determinants in relation to tetracycline residues in chicken.

    abstract：OBJECTIVE:To screen for Escherichia coli (E. coli) resistant to tetracycline, followed by identification of tet efflux genes by polymerase chain reaction (PCR). In addition, detection of tetracycline residues in chicken livers and kidneys were conducted using high performance liquid chromatography-tandem quadrupole mas...

    journal_title：Asian Pacific journal of tropical medicine

    pub_type： 杂志文章

    doi：10.1016/S1995-7645(13)60125-X

    authors： S N AB,B M AM,A S AA,A E E,I Y M

    更新日期：2013-09-01 00:00:00

  • Mosquito larvicidal and ovicidal properties of Eclipta alba (L.) Hassk (Asteraceae) against chikungunya vector, Aedes aegypti (Linn.) (Diptera: Culicidae).

    abstract：OBJECTIVE:The present study deals with the investigation of larvicidal and ovicidal activities of benzene, hexane, ethyl acetate, methanol and chloroform leaf extract of Eclipta alba (E. alba) against dengue vector, Aedes aegypti (Ae. Aegypti). METHODS:Twenty five early III instar larvae of Ae. aegypti was exposed to ...

    journal_title：Asian Pacific journal of tropical medicine

    pub_type： 杂志文章

    doi：10.1016/S1995-7645(11)60026-6

    authors： Govindarajan M,Karuppannan P

    更新日期：2011-01-01 00:00:00

  • Harmony in health sector: a requirement for effective healthcare delivery in Nigeria.

    abstract：:Harmony is defined as the pleasing combination of elements of a system to form an all-inclusive, all involving and more productive team. The aim of this present review was to investigate the factors militating against harmony among healthcare professional in the Nigerian healthcare delivery system. This review was car...

    journal_title：Asian Pacific journal of tropical medicine

    pub_type： 杂志文章

    doi：10.1016/S1995-7645(14)60196-6

    authors： Osaro E,Charles AT

    更新日期：2014-09-01 00:00:00

  • Effects of Gmelina arborea extract on experimentally induced diabetes.

    abstract：OBJECTIVE:To study the effects of aqueous extract of Gmelina arborea bark on normoglycemic levels and streptozotocin (STZ) induced diabetes in rats. METHODS:After single administration of the aqueous extract, plasma glucose level was determined up to 6 h. In subacute study, the aqueous extract was administered for 28 ...

    journal_title：Asian Pacific journal of tropical medicine

    pub_type： 杂志文章

    doi：10.1016/S1995-7645(13)60104-2

    authors： Kulkarni YA,Veeranjaneyulu A

    更新日期：2013-08-01 00:00:00

  • Publication patterns and citation analysis of APJTM during 2008 and June 2014.

    abstract：OBJECTIVE:To draw on data about publication patterns and citation indicators of Asian Pacific Journal of Tropical Medicine (APJTM) during 2008 and June 2014 in order to know about the current state of the journal. METHODS:Data used in this study were collected based on publications in APJTM from 2008 to June, 2014. In...

    journal_title：Asian Pacific journal of tropical medicine

    pub_type： 杂志文章

    doi：10.1016/S1995-7645(14)60109-7

    authors： Lei Y,Tan BJ,Zou Z,Zhang MM,Song RP,Qu SH,Li J

    更新日期：2014-08-01 00:00:00

  • Protective effect of omeprazole on gastric mucosal of cirrhotic portal hypertension rats.

    abstract：OBJECTIVE:To observe the protective effect of omeprazole on gastric mucosal of cirrhotic portal hypertension rats. METHODS:All rats were randomly divided into normal control group, cirrhosis and treatment group. Thioacetamide was used to establish rat model of cirrhotic portal hypertension. The necrotic tissue of gast...

    journal_title：Asian Pacific journal of tropical medicine

    pub_type： 杂志文章

    doi：10.1016/S1995-7645(14)60065-1

    authors： Gao W,Li HY,Wang LX,Hao LJ,Gao JL,Zheng RJ,Cai CJ,Si YL

    更新日期：2014-05-01 00:00:00

  • Seasonal variations in health indices of free-ranging asymptomatic guinea fowls (Numida meleagris) in Zambia.

    abstract：OBJECTIVE:To determine the impact of seasonal variations on health indices of free-ranging asymptomatic guinea fowls (Numida meleagris) in Zambia. METHODS:A time series analysis was carried out on a prospective cohort study over a 12 month period between March 2010 and February 2011 by examining a total 147 guinea fow...

    journal_title：Asian Pacific journal of tropical medicine

    pub_type： 杂志文章

    doi：10.1016/S1995-7645(14)60221-2

    authors： Nalubamba KS,Mudenda NB,Bwalya EC,Masuku M,Munyeme M,Munang'andu HM

    更新日期：2014-09-01 00:00:00

  • Pulmonary sequestration infected with nontuberculous mycobacteria: a report of two cases and literature review.

    abstract：:We report two cases of pulmonary sequestration infected with nontuberculous mycobacteria (NTM): Mycobacterium avium and Mycobacterium abscessus. Chest computed tomography showed pneumonic consolidation in the right lower lobe, which received a systemic blood supply from the descending aorta in both patients. Video-ass...

    journal_title：Asian Pacific journal of tropical medicine

    pub_type： 杂志文章,评审

    doi：10.1016/S1995-7645(12)60172-2

    authors： Koh WJ,Hong G,Kim K,Ahn S,Han J

    更新日期：2012-11-01 00:00:00

  • Study on prevention effect of Zishen Yutai pill combined with progesterone for threatened abortion in rats.

    abstract：OBJECTIVE:To observe preventive and therapeutic effects and the mechanism of actions on Zishen Yutai pill combined with progesterone on threatened abortion in rats. METHODS:After pregnancy, 50 SPF female SD rats were selected and divided into control group, model group, progesterone group, Zishen Yutai pill group, and...

    journal_title：Asian Pacific journal of tropical medicine

    pub_type： 杂志文章

    doi：10.1016/j.apjtm.2016.04.002

    authors： Zhang Y,Yan W,Ge PF,Li Y,Ye Q

    更新日期：2016-06-01 00:00:00

  • Suppressive effect of pectic polysaccharides extracted from Rauwolfia verticillata (Lour.) Baill.var.hainanensis Tsiang on inflammation by regulation of NF- κ B pathway and interleukin-17 in mice with dextran sulphatesodium-induced ulcerative colitis.

    abstract：OBJECTIVE:To investigate the effects of pectic polysaccharides extracted from Rauwolfia verticillata (Lour.) Baill.var.hainanensis Tsiang on an experimental murine colitis model. METHODS:Experimental colitis was induced by dextran sulfate sodium (DSS), and mice were divided into 4 groups: control, DSS alone, DSS plus ...

    journal_title：Asian Pacific journal of tropical medicine

    pub_type： 杂志文章

    doi：10.1016/S1995-7645(14)60306-0

    authors： Miao XP,Sun XN,Cui LJ,Cao QF,Zhuang GF,Deng TZ,Zhang DY

    更新日期：2015-02-01 00:00:00

  • Current perspectives on dengue episode in Malaysia.

    abstract：:Prevalence of dengue transmission has been alarmed by an estimate of 390 million infections per annum. Urban encroachment, ecological disruption and poor sanitation are all contributory factors of increased epidemiology. Complication however arises from the fact that dengue virus inherently exists as four different se...

    journal_title：Asian Pacific journal of tropical medicine

    pub_type： 杂志文章,评审

    doi：10.1016/j.apjtm.2016.03.004

    authors： Pang EL,Loh HS

    更新日期：2016-04-01 00:00:00

  • Clostridium difficile infection: a review of the literature.

    abstract：:Clostridium difficile is a Gram-positive, strictly anaerobic, spore-forming bacterium. It is the most common cause of antibiotic-associated diarrhea in hospitals and other healthcare facilities and is of significant concern because of the increasing morbidity and mortality rates as well as increased health care costs....

    journal_title：Asian Pacific journal of tropical medicine

    pub_type： 杂志文章

    doi：10.1016/S1995-7645(14)60197-8

    authors： Khan FY,Elzouki AN

    更新日期：2014-09-01 00:00:00

  • Role of Moringa oleifera in regulation of diabetes-induced oxidative stress.

    abstract：OBJECTIVE:To evaluate the antioxidant activity of aqueous extract of Moringa oleifera (M. oleifera) young leaves by in vivo as well as in vitro assays. METHODS:In vitro study included estimation of total phenolic, total flavonol, total flavonoid and total antioxidant power (FRAP assay). In addition, in vivo study was ...

    journal_title：Asian Pacific journal of tropical medicine

    pub_type： 杂志文章

    doi：10.1016/S1995-7645(13)60068-1

    authors： Jaiswal D,Rai PK,Mehta S,Chatterji S,Shukla S,Rai DK,Sharma G,Sharma B,Khair S,Watal G

    更新日期：2013-06-01 00:00:00

  • Expression of TRAP1 in gastric cancer tissue and its correlation with malignant biology.

    abstract：OBJECTIVE:To study the expression of tumor necrosis factor receptor-associated protein 1 (TRAP1) in gastric cancer tissue and its correlation with malignant biology. METHODS:Gastric cancer tissue and adjacent normal tissue were collected, and mRNA content and protein content of TRAP1 were detected; gastric cancer cell...

    journal_title：Asian Pacific journal of tropical medicine

    pub_type： 杂志文章

    doi：10.1016/j.apjtm.2015.12.014

    authors： Han P,Wang QL,Zhang X

    更新日期：2016-01-01 00:00:00

  • Molecular cloning of virB12 gene of Brucella melitensis 16M strain in pET28a vector.

    abstract：OBJECTIVE:To clone the virB12 gene in pET28a expression vector for production of recombinant protein to be used as antigenic component for future serological test development. METHODS:Brucella melitensis (B. melitensis) 16M strain was cultured and bacterial DNA was extracted by Bioneer AccuPrep® Genomic DNA Extraction...

    journal_title：Asian Pacific journal of tropical medicine

    pub_type： 杂志文章

    doi：10.1016/S1995-7645(12)60089-3

    authors： Mirkalantari S,Amirmozafari N,Kazemi B,Irajian G

    更新日期：2012-07-01 00:00:00

  • Efficacy evaluation of the protein isolated from Peganum harmala seeds as an antioxidant in liver of rats.

    abstract：OBJECTIVE:To evaluate the curative effect of the 132 KD protein isolated from the seeds of Peganum harmala (P. harmala) L. against carbon tetrachloride (CCl(4)) induced oxidative stress in rats. METHODS:Animals were post treated intraperitoneally with 132 KD isolated protein at doses of 4 and 8 mg/kg body weight and b...

    journal_title：Asian Pacific journal of tropical medicine

    pub_type： 杂志文章

    doi：10.1016/S1995-7645(13)60058-9

    authors： Soliman AM,Abu-El-Zahab HS,Alswiai GA

    更新日期：2013-04-13 00:00:00

  • Efficacy evaluation and kinetic study of biosorption of nickel and zinc by bacteria isolated from stressed conditions in a bubble column.

    abstract：OBJECTIVE:To investigate the biosorption potential of isolated bacteria as an alternative biosorbent material for the removal of zinc and nickel from aqueous solution in a bubble column bioreactor. METHORDS:In this study from four points of waste water treatment plant, some Gram-positive and Gram-negative bacteria und...

    journal_title：Asian Pacific journal of tropical medicine

    pub_type： 杂志文章

    doi：10.1016/S1995-7645(14)60231-5

    authors： Arjomandzadegan M,Rafiee P,Moraveji MK,Tayeboon M

    更新日期：2014-09-01 00:00:00

  • RETRACTED: Effect of thioredoxin-interacting protein on Wnt/β-catenin signaling pathway and diabetic myocardial infarction.

    abstract：OBJECTIVE:To explore the regulatory role of thioredoxin-interacting protein (TXNIP) in Wnt/β-catenin signaling pathway and therefore to elucidate its function in diabetic myocardial infarction. METHODS:Diabetic myocardial infarction models were generated in mice. The expression levels of TXNIP and β-catenin and level ...

    journal_title：Asian Pacific journal of tropical medicine

    pub_type： 杂志文章,撤回出版物

    doi：10.1016/j.apjtm.2015.10.010

    authors： Yu H,Zhao XX,Shan XH,Li P,Chen T

    更新日期：2015-11-01 00:00:00

  • Antidiarrheal activity of Pterocarpus erinaceus methanol leaf extract in experimentally-induced diarrhea.

    abstract：OBJECTIVE:To investigate the antidiarrheal activity of the methanol leaf extract of Pterocarpus erinaceus in vivo. METHODS:The methanol leaf extract of Pterocarpus erinaceus was evaluated using different doses (100, 200 and 400 mg/kg body weight) orally for antidiarrheal activity using castor oil-induced diarrhea, cha...

    journal_title：Asian Pacific journal of tropical medicine

    pub_type： 杂志文章

    doi：10.1016/S1995-7645(12)60014-5

    authors： Ezeja IM,Ezeigbo II,Madubuike KG,Udeh NE,Ukweni IA,Akomas SC,Ifenkwe DC

    更新日期：2012-02-01 00:00:00

  • Lentivirus vectors construction of SiRNA targeting interference GPC3 gene and its biological effects on liver cancer cell lines Huh-7.

    abstract：OBJECTIVES:To build GPC3 gene short hairpin interference RNA (shRNA) slow virus vector, observe expression of Huh-7 GPC3 gene in human liver cell line proliferation apoptosis and the effect of GPC3 gene influencing on liver cancer cell growth, and provide theoretical basis for gene therapy of liver cancer. METHODS:Hep...

    journal_title：Asian Pacific journal of tropical medicine

    pub_type： 杂志文章

    doi：10.1016/S1995-7645(14)60136-X

    authors： Lei CJ,Yao C,Pan QY,Long HC,Li L,Zheng SP,Zeng C,Huang JB

    更新日期：2014-10-01 00:00:00

  • Free radical scavenging property and antiproliferative activity of Rhodiola imbricata Edgew extracts in HT-29 human colon cancer cells.

    abstract：OBJECTIVE:To investigate the in vitro antioxidant and antiproliferative activity of rhizome extracts of Rhodiola imbricata (R. imbricata) in HT-29 human colon cancer cell line. METHODS:The successively extracted rhizome of R. imbricata using various solvents was analyzed for their total phenolics, tannins and flavonoi...

    journal_title：Asian Pacific journal of tropical medicine

    pub_type： 杂志文章

    doi：10.1016/S1995-7645(12)60194-1

    authors： Senthilkumar R,Parimelazhagan T,Chaurasia OP,Srivastava RB

    更新日期：2013-01-01 00:00:00

  • RETRACTED: Effect of transplantation of BMMSCs on pathological change of gastric precancerous lesions of rats.

    abstract：OBJECTIVE:To build the rat model of gastric precancerous lesions and discuss the effect of transplantation of mesenchymal stem cells (BMMSCs) on the pathological change. METHODS:The rat model of gastric precancerous lesions was built using N-methyl-N'-nitro-N'-nitrosoguanidine. After the intravenous transplantation of...

    journal_title：Asian Pacific journal of tropical medicine

    pub_type： 杂志文章,撤回出版物

    doi：10.1016/j.apjtm.2015.11.006

    authors： Lin ZL,Zheng GW,Zhang L,Zheng JT,Chen H

    更新日期：2015-12-01 00:00:00

  • GC-MS analysis of volatile compounds of Perilla frutescens Britton var. Japonica accessions: Morphological and seasonal variability.

    abstract：OBJECTIVE:To investigate the composition of volatile compounds in the different accessions of Perilla frutescens (P. frutescens) collected from various habitats of China and Japan. METHODS:In the present study, the essential oil from the leaves of P. frutescens cultivars from China and Japan was extracted by hydro-dis...

    journal_title：Asian Pacific journal of tropical medicine

    pub_type： 杂志文章

    doi：10.1016/j.apjtm.2017.07.004

    authors： Ghimire BK,Yoo JH,Yu CY,Chung IM

    更新日期：2017-07-01 00:00:00

  • Comparison on serum biomarkers for anovulatory and ovulatory dysfunctional uterine bleeding in Lizu females.

    abstract：OBJECTIVE:To screen, identify, and compare the serum biomarkers between anovulatory dysfunctional uterine bleeding (ADUB) and ovulatory dysfunctional uterine bleeding (ODUB) in Lizu females. METHODS:The subjects included 128 ADUB patients, 63 ODUB patients, and 93 controls. The serum and supernate of the subjects' men...

    journal_title：Asian Pacific journal of tropical medicine

    pub_type： 杂志文章

    doi：10.1016/S1995-7645(14)60012-2

    authors： Bao S,Yang SY,Li ZR,Wen GB

    更新日期：2014-02-01 00:00:00

  • Profile and geographical distribution of reported cutaneous leishmaniasis cases in Northwestern Saudi Arabia, from 2010 to 2013.

    abstract：OBJECTIVE:To describe the profile and geographical distribution of reported cases of cutaneous leishmaniasis (CL) in the province of Hail, Saudi Arabia from 2010 to 2013. METHODS:Human data were collected from the dermatology service of both King Khaled and General Hail hospitals. RESULTS:A total of 483 confirmed CL ...

    journal_title：Asian Pacific journal of tropical medicine

    pub_type： 杂志文章

    doi：10.1016/S1995-7645(14)60332-1

    authors： Haouas N,Amer O,Ishankyty A,Alazmi A,Ishankyty I

    更新日期：2015-04-01 00:00:00

  • Effect of Rheum palmatum L. on NF-κB signaling pathway of mice with acute liver failure.

    abstract：OBJECTIVE:To explore the regulation effect of Rheum palmatum (R. palmatum) L. on NF-κB signaling pathway of ALF mice. METHODS:The intraperitoneal injection of d-GalN/LPS was employed for the model building. Mice in the treatment group and positive control group were given the R. palmatum L. and bifendate before the mo...

    journal_title：Asian Pacific journal of tropical medicine

    pub_type： 杂志文章

    doi：10.1016/j.apjtm.2015.09.011

    authors： Zhang RZ,Qiu H,Wang N,Long FL,Mao DW

    更新日期：2015-10-01 00:00:00

  • Effects and mechanism of miR-214 on hepatocellular carcinoma.

    abstract：OBJECTIVE:To explore the role of miR-214 in the progression of hepatocellular carcinoma (HCC) and its inhibitory mechanisms in depressing the signaling pathway of β-catenin, this study was conducted. METHODS:We ectopically expressed miR-214 in HepG2 cells to obtain cell lines Lv-miR-214-HepG2 and their control Lv-cont...

    journal_title：Asian Pacific journal of tropical medicine

    pub_type： 杂志文章

    doi：10.1016/S1995-7645(14)60350-3

    authors： Zhang LL,Guo YJ,Zhao CN,Gao JY

    更新日期：2015-05-01 00:00:00