Abstract：
:Streptomyces lydicus A02 is used by industry because it has a higher natamycin-producing capacity than the reference strain S. natalensis ATCC 27448. We sequenced the complete genome of A02 using next-generation sequencing platforms, and to achieve better sequence coverage and genome assembly, we utilized single-molecule real-time (SMRT) sequencing. The assembled genome comprises a 9,307,519-bp linear chromosome with a GC content of 70.67%, and contained 8,888 predicted genes. Comparative genomics and natamycin biosynthetic gene cluster (BGC) analysis showed that BGC are highly conserved among evolutionarily diverse strains, and they also shared closer genome evolution compared with other Streptomyces species. Forty gene clusters were predicted to involve in the secondary metabolism of A02, and it was richly displayed in two-component signal transduction systems (TCS) in the genome, indicating a complex regulatory systems and high diversity of metabolites. Disruption of the phoP gene of the phoR-phoP TCS and nsdA gene confirmed phosphate sensitivity and global negative regulation of natamycin production. The genome sequence and analyses presented in this study provide an important molecular basis for research on natamycin production in Streptomyces, which could facilitate rational genome modification to improve the industrial use of A02.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Wu H,Liu W,Shi L,Si K,Liu T,Dong D,Zhang T,Zhao J,Liu D,Tian Z,Yue Y,Zhang H,Xuelian B,Liang Ydoi
10.1038/s41598-017-09532-3subject
Has Abstractpub_date
2017-08-22 00:00:00pages
9114issue
1issn
2045-2322pii
10.1038/s41598-017-09532-3journal_volume
7pub_type
杂志文章abstract：:Northern and tropical peatlands represent a globally significant carbon reserve accumulated over thousands of years of waterlogged conditions. It is unclear whether moderate drying predicted for northern peatlands will stimulate burning and carbon losses as has occurred in their smaller tropical counterparts where the...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep08063
更新日期：2015-01-27 00:00:00
abstract：:Lipoprotein associated phospholipase A2 (Lp-PLA2) has been characterized for its interfacial activation as well as inhibition by detergent micelles and lipoprotein particles. The enzyme has been shown to bind on the surfaces of hydrophobic aggregates, such as detergent micelles, lipoprotein particles and even polystyr...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-74236-0
更新日期：2020-10-14 00:00:00
abstract：:Exosomes contain cell-specific collections of bioactive materials including proteins, lipids, and RNAs that are transported to recipient cells to exert their impacts. MicroRNAs (miRNAs) can function as tumor suppressor or oncogenic genes and miR-21 is one of the most frequently up-regulated miRNAs in solid tumors incl...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-65207-6
更新日期：2020-05-19 00:00:00
abstract：:High efficiency polymer:fullerene photovoltaic device layers self-assemble with hierarchical features from ångströms to 100's of nanometers. The feature size, shape, composition, orientation, and order all contribute to device efficiency and are simultaneously difficult to study due to poor contrast between carbon bas...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep30915
更新日期：2016-08-08 00:00:00
abstract：:Cells harboring latent HIV-1 pose a major obstacle to eradication of the virus. The 'shock and kill' strategy has been broadly explored to purge the latent reservoir; however, none of the current latency-reversing agents (LRAs) can safely and effectively activate the latent virus in patients. In this study, we report ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-07157-0
更新日期：2017-08-25 00:00:00
abstract：:Graphene has been identified as an emerging horizon for a nanoscale photonic platform because the Fermi level of intrinsic graphene can be engineered to support surface plasmons (SPs). The current solid back electrical gating and chemical doping methods cannot facilitate the demonstration of graphene SPs at the near-i...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep06559
更新日期：2014-10-09 00:00:00
abstract：:A polymer coating as polycaprolactone (PCL) is applied to improve the initial corrosion resistance of biodegradable magnesium. In addition, plasma electrolytic oxidation (PEO) is performed to increase adhesion between the polymer and the metal. However, when a complex-shaped material such as a screw is implanted in a ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-31359-9
更新日期：2018-09-05 00:00:00
abstract：:Fuelled by a desire for greater connectivity, networked systems now pervade our society at an unprecedented level that will affect it in ways we do not yet understand. In contrast, nature has already developed efficient networks that can instigate rapid response and consensus when key elements are stimulated. We prese...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-53942-4
更新日期：2019-11-26 00:00:00
abstract：:Metabolomics has emerged as a powerful approach to comprehensively interrogate cellular biochemistry. As such, we applied an untargeted liquid chromatography-mass spectrometry metabolomic strategy to elucidate metabolome changes in the anthracnose-causing hemibiotrophic sorghum pathogen, Colletotrichum sublineolum. An...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-38692-7
更新日期：2019-03-01 00:00:00
abstract：:miRNAs play an indispensable role in human carcinogenesis. Dysregulated miR-1180-3p has been observed in several types of cancer, including hepatocellular carcinoma (HCC). This study intends to correlate the expression level of miR-1180-3p with clinical features and overall survival in HCC patients. The expression and...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-68450-z
更新日期：2020-07-14 00:00:00
abstract：:The magnetization process is a very important probe to study magnetic materials, particularly in search of spin-liquid states in quantum spin systems. Regrettably, however, progress of the theoretical analysis has been unsatisfactory, mostly because it is hard to obtain sufficient numerical data to support the theory....
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-70389-0
更新日期：2020-08-26 00:00:00
abstract：:MicroRNAs (miRNAs) have been considered to be potent biomarkers for early disease diagnosis and for cancer therapy. The rapid and selective detection of miRNAs without reverse transcription and labelling is highly desired. Herein, we report a simple and label-free miRNA detection method that is based on the Duplex-Spe...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep07400
更新日期：2014-12-10 00:00:00
abstract：:Plant genomes encode several classes of small regulatory RNAs (sRNAs) that play critical roles in both development and stress responses. Panax notoginseng (Burk.) F.H. Chen (P. notoginseng) is an important traditional Chinese herbal medicinal plant species for its haemostatic effects. Therefore, the root yield of P. n...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-09670-8
更新日期：2017-08-25 00:00:00
abstract：:Tropical deforestation is responsible for around one tenth of total anthropogenic carbon emissions, and tropical protected areas (PAs) that reduce deforestation can therefore play an important role in mitigating climate change and protecting biodiversity and ecosystem services. While the effectiveness of PAs in reduci...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-14467-w
更新日期：2017-10-25 00:00:00
abstract：:In utero and upon delivery, neonates are exposed to a wide array of microorganisms from various sources, including maternal bacteria. Prior studies have proposed that the mode of feeding shapes the gut microbiota and, subsequently the child's health. However, the effect of the mode of feeding and its influence on the ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep38309
更新日期：2016-12-06 00:00:00
abstract：:The maternal environment can influence the intensity of seed dormancy and thus seasonal germination timing and post-germination life history traits. We tested the hypotheses that germination season influences phenotypic expression of post-germination life history traits in the cold desert annual Isatis violascens and ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep25076
更新日期：2016-04-27 00:00:00
abstract：:The transition to agriculture was a key event in human history. The extent to which this transition is associated with biological changes in different world regions remains debated. Cultural and osteological records in Lower Nubia throughout the Holocene have been interpreted as a result of in situ differentiation or ...
journal_title：Scientific reports
pub_type： 历史文章,杂志文章
doi：10.1038/srep31040
更新日期：2016-08-09 00:00:00
abstract：:This study investigates the effect of Repetitive Transcranial Magnetic Stimulation (rTMS) on persistent post-concussion syndrome (PCS). The study design was a randomized (coin toss), placebo controlled, and double-blind study. Thirty-seven participants with PCS were assessed for eligibility; 22 were randomised and 18 ...
journal_title：Scientific reports
pub_type： 杂志文章,随机对照试验
doi：10.1038/s41598-019-41923-6
更新日期：2019-04-02 00:00:00
abstract：:The human connectome has become the very frequent subject of study of brain-scientists, psychologists and imaging experts in the last decade. With diffusion magnetic resonance imaging techniques, united with advanced data processing algorithms, today we are able to compute braingraphs with several hundred, anatomicall...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-68914-2
更新日期：2020-07-20 00:00:00
abstract：:Systemic Sclerosis (SSc) is a disease with limited therapeutic possibilities. Mesenchymal stem cells (MSCs)-therapy could be a promising therapeutic option, however the ideal MSCs source has not yet been found. To address this problem, we perform comparison between bone marrow (BM)-MSCs and adipose (A)-MSCs, by the mi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-43638-0
更新日期：2019-05-09 00:00:00
abstract：:Animals thriving in hot deserts rely on extraordinary adaptations and thermoregulatory capacities to cope with heat. Uncovering such adaptations, and how they may be favoured by selection, is essential for predicting climate change impacts. Recently, the arid-adapted zebra finch was discovered to program their offspri...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-75909-6
更新日期：2020-11-03 00:00:00
abstract：:The carrier transport characteristics of Sb2Se2Te topological insulators were investigated, after exposure to different levels of nitrogen gas. The magnetoresistance (MR) slope for the Sb2Se2Te crystal increased by approximately 100% at 10 K after 2-days of exposure. The Shubnikov-de Haas (SdH) oscillation amplitude i...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-05369-y
更新日期：2017-07-11 00:00:00
abstract：:Hedgehog (Hh) signaling plays a broad role in the development of many organs including bone and teeth. It is noted that sustained Hh activity in osteoblasts negatively regulates postnatal development in mice. However, it remains unknown whether Hh signaling contributes to cementum formation. In this study, to define t...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-64188-w
更新日期：2020-04-29 00:00:00
abstract：:Floral transition and petal onset, as two main aspects of flower development, are crucial to rapeseed evolutionary success and yield formation. Currently, very little is known regarding the genetic architecture that regulates flowering time and petal morphogenesis in Brassica napus. In the present study, a genome-wide...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep30576
更新日期：2016-07-27 00:00:00
abstract：:Recognising an object involves rapid visual processing and activation of semantic knowledge about the object, but how visual processing activates and interacts with semantic representations remains unclear. Cognitive neuroscience research has shown that while visual processing involves posterior regions along the vent...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-28865-1
更新日期：2018-07-13 00:00:00
abstract：:We report on neutron diffraction, thermal expansion, magnetostriction, dielectric, and specific heat measurements on polycrystalline FeCr2S4 in external magnetic fields. The ferrimagnetic ordering temperatures TC ≈ 170 K and the transition at TOO ≈ 10 K, which has been associated with orbital ordering, are only weakly...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep06079
更新日期：2014-08-15 00:00:00
abstract：:We have engineered the electronic structure at the interface between Cu2O and ZnO nanorods (NRs) array, through adjusting the carrier concentration of Cu2O. The electrodeposition of Cu2O at pH 11 acquired the highest carrier concentration, resulting in the largest interfacial electric field between Cu2O and ZnO, which...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep07882
更新日期：2015-01-20 00:00:00
abstract：:Little is known about circulating biomarkers of vascular injury in relation to cardiovascular disease risk. Thus, we evaluated associations between six novel markers (E-Selectin, P-Selectin, thrombomodulin, thrombopoietin, intercellular adhesion molecule 3 and GPIIb/IIIa) and established cardiovascular risk factors as...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-38259-y
更新日期：2019-02-28 00:00:00
abstract：:This study aimed to examine hemodynamic responses and the necessity of breaks under long working hours. Thirty-eight healthy males conducted PC-based work from 9:10 to 22:00. Nine 10-minute short breaks and two long breaks (a 1-hour break and a 50-minute break) were provided, and hemodynamic responses were measured re...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-32908-y
更新日期：2018-09-28 00:00:00
abstract：:Attaching Unique Molecular Identifiers (UMI) to RNA molecules in the first step of sequencing library preparation establishes a distinct identity for each input molecule. This makes it possible to eliminate the effects of PCR amplification bias, which is particularly important where many PCR cycles are required, for e...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-31064-7
更新日期：2018-09-03 00:00:00
