Abstract：
:Precision Medicine in Oncology requires tailoring of therapeutic strategies to individual cancer patients. Due to the limited quantity of tumor samples, this proves to be difficult, especially for early stage cancer patients whose tumors are small. In this study, we exploited a 2.4 × 2.4 centimeters polydimethylsiloxane (PDMS) based microfluidic chip which employed droplet microfluidics to conduct drug screens against suspended and adherent cancer cell lines, as well as cells dissociated from primary tumor of human patients. Single cells were dispersed in aqueous droplets and imaged within 24 hours of drug treatment to assess cell viability by ethidium homodimer 1 staining. Our results showed that 5 conditions could be screened for every 80,000 cells in one channel on our chip under current circumstances. Additionally, screening conditions have been adapted to both suspended and adherent cancer cells, giving versatility to potentially all types of cancers. Hence, this study provides a powerful tool for rapid, low-input drug screening of primary cancers within 24 hours after tumor resection from cancer patients. This paves the way for further technological advancement to cutting down sample size and increasing drug screening throughput in advent to personalized cancer therapy.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Wong AH,Li H,Jia Y,Mak PI,Martins RPDS,Liu Y,Vong CM,Wong HC,Wong PK,Wang H,Sun H,Deng CXdoi
10.1038/s41598-017-08831-zsubject
Has Abstractpub_date
2017-08-22 00:00:00pages
9109issue
1issn
2045-2322pii
10.1038/s41598-017-08831-zjournal_volume
7pub_type
杂志文章abstract：:Specific HPV genotypes have been recognized as risk factors inducing head and neck cancers (HNC). The aim of this study was to validate a real-time PCR assay to detect accurately High Risk HPV DNA in Formalin Fixed Paraffin Embedded (FFPE) and oral cytobrush samples and compare the results with conventional PCR. Repea...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-29790-z
更新日期：2018-07-27 00:00:00
abstract：:Early treatment, soon after infection, reduces HIV transmissions and benefits patients. The Transmission Reduction Intervention Project (TRIP) evaluated a network intervention to detect individuals recently infected (in the past 6 months). TRIP was conducted in Greece (2013-2015) and focused on drug injector networks....
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep38100
更新日期：2016-12-05 00:00:00
abstract：:Traffic-related air pollution is known to be associated with skin aging manifestations. We previously found that the use of fossil fuels was associated with skin aging, but no direct link between indoor air pollutants and skin aging manifestations has ever been shown. Here we directly measured the indoor PM2.5 exposur...
journal_title：Scientific reports
pub_type： 临床试验,杂志文章
doi：10.1038/s41598-017-15295-8
更新日期：2017-11-10 00:00:00
abstract：:Application of PCR to multiplexing assays is not trivial; it requires multiple fluorescent labels for amplicon detection and sophisticated software for data interpretation. Alternative PCR-free methods exploiting new concepts in nanotechnology exhibit high sensitivities but require multiple labeling and/or amplificati...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep02033
更新日期：2013-01-01 00:00:00
abstract：:Extracellular vesicles derived from mesenchymal stem cells (MSCs) represent a novel approach for regenerative and immunosuppressive therapy. Recently, cytochalasin B-induced microvesicles (CIMVs) were shown to be effective drug delivery mediators. However, little is known about their immunological properties. We propo...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-67563-9
更新日期：2020-07-01 00:00:00
abstract：:We describe the realization and characterization of a compact, autonomous fiber laser system that produces the optical frequencies required for laser cooling, trapping, manipulation, and detection of 87Rb atoms - a typical atomic species for emerging quantum technologies. This device, a customized laser system from th...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-59971-8
更新日期：2020-02-24 00:00:00
abstract：:The neural substrates by which speech sounds are perceptually segregated into distinct streams are poorly understood. Here, we recorded high-density scalp event-related potentials (ERPs) while participants were presented with a cyclic pattern of three vowel sounds (/ee/-/ae/-/ee/). Each trial consisted of an adaptatio...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep40790
更新日期：2017-01-19 00:00:00
abstract：:Surface-enhanced Raman spectroscopy (SERS) has attracted a lot of attention in molecular sensing because of the remarkable ability of plasmonic metal nanostructures to enhance the weak Raman scattering process. On the other hand, coherent vibrational spectroscopy triggered by impulsive excitation using ultrafast laser...
journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2016-11-04 00:00:00
abstract：:This study evaluates the forensic utility of 23 autosomal short tandem repeat markers in 400 samples from the Kuwaiti population, of which four markers (D10S1248, D22S1045, D2S441 and SE33) are reported for the first time for Kuwait. All the markers were shown to exhibit no deviation from Hardy-Weinberg equilibrium, n...
journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2021-01-21 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2017-03-10 00:00:00
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journal_title：Scientific reports
pub_type： 临床试验,杂志文章,多中心研究
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更新日期：2020-02-28 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2019-11-21 00:00:00
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pub_type： 杂志文章
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更新日期：2020-06-17 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-33450-7
更新日期：2018-11-01 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2019-12-17 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep31502
更新日期：2016-08-17 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-00264-y
更新日期：2017-03-14 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2019-12-27 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep06945
更新日期：2014-11-06 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2017-07-25 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2020-03-20 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2020-12-16 00:00:00
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pub_type： 杂志文章
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更新日期：2016-08-11 00:00:00
abstract：:A new approach to estimate the Shannon entropy of a long-range correlated sequence is proposed. The entropy is written as the sum of two terms corresponding respectively to power-law (ordered) and exponentially (disordered) distributed blocks (clusters). The approach is illustrated on the 24 human chromosome sequences...
journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2013-01-01 00:00:00
abstract：:The helicene is constructed by twisted benzene or other aromatic rings, exhibiting a helical structure. Using first-principles calculations, we investigate the electronic transport of helicenes under stretching or compressing. Interestingly, a U-shaped curve of the current against d (the pitch of a helicene) is observ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep16731
更新日期：2015-11-19 00:00:00
abstract：:The decreasing size of modern functional magnetic materials and devices cause a steadily increasing demand for high resolution quantitative magnetic characterization. Transmission electron microscopy (TEM) based measurements of the electron energy-loss magnetic chiral dichroism (EMCD) may serve as the needed experimen...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-01077-9
更新日期：2017-04-19 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2017-04-03 00:00:00
abstract：:The cancer stem cell (CSC) hypothesis proposes a hierarchical organization of tumors, in which stem-like cells sustain tumors and drive metastasis. The molecular mechanisms underlying the acquisition of CSCs and metastatic traits are not well understood. SOX9 is a transcription factor linked to stem cell maintenance a...
journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2016-08-30 00:00:00
abstract：:The newly discovered Candidatus 'Methanoperedens nitroreducens' (M. nitroreducens), mediating nitrate-dependent anaerobic oxidation of methane, is an important microorganism in linking carbon and nitrogen cycles. In order to explore the diversity of M. nitroreducens-like archaea in various environmental niches with ad...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-24974-z
更新日期：2018-05-16 00:00:00
