Abstract：
:Although cisplatin plays a central role in cancer chemotherapy, the mechanisms of cell response to this drug have been unexplored. The present study demonstrates the relationships between the intracellular pH (pHi), cell bioenergetics and the response of cervical cancer to cisplatin. pHi was measured using genetically encoded sensor SypHer2 and metabolic state was accessed by fluorescence intensities and lifetimes of endogenous cofactors NAD(P)H and FAD. Our data support the notion that cisplatin induces acidification of the cytoplasm early after the treatment. We revealed in vitro that a capacity of cells to recover and maintain alkaline pHi after the initial acidification is the crucial factor in mediating the cellular decision to survive and proliferate at a vastly reduced rate or to undergo cell death. Additionally, we showed for the first time that pHi acidification occurs after prolonged therapy in vitro and in vivo, and this, likely, favors metabolic reorganization of cells. A metabolic shift from glycolysis towards oxidative metabolism accompanied the cisplatin-induced inhibition of cancer cell growth in vitro and in vivo. Overall, these findings contribute to an understanding of the mechanisms underlying the responsiveness of an individual cell and tumor to therapy and are valuable for developing new therapeutic strategies.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Shirmanova MV,Druzhkova IN,Lukina MM,Dudenkova VV,Ignatova NI,Snopova LB,Shcheslavskiy VI,Belousov VV,Zagaynova EVdoi
10.1038/s41598-017-09426-4subject
Has Abstractpub_date
2017-08-21 00:00:00pages
8911issue
1issn
2045-2322pii
10.1038/s41598-017-09426-4journal_volume
7pub_type
杂志文章abstract：:Heavy metals are environmental pollutants affect the integrity and distribution of living organisms in the ecosystem and also humans across the food chain. The study targeted the removal of copper (Cu2+) from aqueous solutions, depending on the biosorption process. The bacterial candidate was identified using 16S rRNA...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-66101-x
更新日期：2020-06-11 00:00:00
abstract：:This study was designed to evaluate the prognostic value of platelet to lymphocyte ratio (PLR) in hepatocellular carcinoma (HCC). A comprehensive literature search for relevant studies was performed in Web of science, Embase and Pubmed. A total of nine studies with 2017 patients were included in this meta-analysis, an...
journal_title：Scientific reports
pub_type： 杂志文章,meta分析
doi：10.1038/srep35378
更新日期：2016-10-14 00:00:00
abstract：:A wet-chemical method combined with spark plasma sintering was used to prepare a W-Y2O3 alloy. High-temperature tensile tests and nano-indentation microhardness tests were used to characterize the mechanical properties of the alloy. After He-ion irradiation, fuzz and He bubbles were observed on the irradiated surface....
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep12755
更新日期：2015-07-31 00:00:00
abstract：:The exact mechanism of endometriosis is unknown. The recommendation system (RS) based on item similarities of machine learning has never been applied to the relationship between diseases. The study aim was to identify diseases associated with endometriosis by applying RS based on item similarities to insurance data in...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-41973-w
更新日期：2019-04-01 00:00:00
abstract：:Mutations in the SNX14 gene cause spinocerebellar ataxia, autosomal recessive 20 (SCAR20) in both humans and dogs. Studies implicating the phenotypic consequences of SNX14 mutations to be consequences of subcellular disruption to autophagy and lipid metabolism have been limited to in vitro investigation of patient-der...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-70797-2
更新日期：2020-08-13 00:00:00
abstract：:Metallic nanostructures are of immense scientific interest owing to unexpectedly strong interaction with light in deep subwavelength scales. Resonant excitations of surface and cavity plasmonic modes mediate strong light localization in nanoscale objects. Nevertheless, the role of surface plasmon-polaritons (SPP) in l...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep05683
更新日期：2014-07-14 00:00:00
abstract：:We introduce multi-gradients including Laplace pressure gradient, wettable gradient and wettable different gradient on a high adhesion surface via special wedge-pattern and improved anodic oxidation method. As a result of the cooperative effect mentioned above, controlled directional motion of a droplet on a high adhe...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep45687
更新日期：2017-04-03 00:00:00
abstract：:Colorectal cancer is one of the main causes of cancer death worldwide, and novel biomarkers are urgently needed for its early diagnosis and treatment. The utilization of metabolomics to identify and quantify metabolites in body fluids may allow the detection of changes in their concentrations that could serve as diagn...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-55952-8
更新日期：2019-12-27 00:00:00
abstract：:Phase transition is a natural phenomenon happened around our daily life, represented by the process from ice to water. While melting and solidifying at a certain temperature, a high heat of fusion is accompanied, classified as the latent heat. Phase change material (PCM) has been widely applied to store and release la...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep08884
更新日期：2015-03-09 00:00:00
abstract：:MRI of hyperpolarized media, such as (129)Xe and (3)He, shows great potential for clinical applications. The optimal use of the available spin polarization requires accurate flip angle calibrations and T1 measurements. Traditional flip angle calibration methods are time-consuming and suffer from polarization losses du...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep25854
更新日期：2016-05-12 00:00:00
abstract：:Crohn's disease (CD) has been associated with an increased consumption of n-6 polyunsaturated fatty acid (PUFA), while greater intake of n-3 PUFA has been associated with a reduced risk. We sought to investigate serum fatty acid composition in CD, and associations of fatty acids with disease activity, cytokines, and a...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-39232-z
更新日期：2019-02-27 00:00:00
abstract：:The development of a miniaturised device that provides efficient beam manipulation with high transmittance is extremely desirable for the broad range of applications including holography, metalens, and imaging. Recently, the potential of dielectric metasurfaces has been unleashed to efficiently manipulate the beam wit...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-42444-y
更新日期：2019-04-24 00:00:00
abstract：:Although stenting of non-branched arterial segments has acceptable clinical outcomes, in-stent restenosis (ISR) and stent thrombosis remain clinically significant issues for vascular bifurcations (15-28% restenosis). Local fluid and solid stresses appear to play an important role in restenosis and thrombosis. The comb...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-66777-1
更新日期：2020-06-15 00:00:00
abstract：:Here, we report large, near-daily oscillations of near-bottom temperatures, with ranges of up to 11.5 °C at depths of 30-60 m in September 2011 around the Saint Pierre and Miquelon archipelago (south-eastern Canada). These oscillations were detected on velocity and temperature profiles from moorings in Miquelon Bay an...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-31857-w
更新日期：2018-09-17 00:00:00
abstract：:Humans interact with the oceans in diverse and profound ways. The scope, magnitude, footprint and ultimate cumulative impacts of human activities can threaten ocean ecosystems and have changed over time, resulting in new challenges and threats to marine ecosystems. A fundamental gap in understanding how humanity is af...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-47201-9
更新日期：2019-08-12 00:00:00
abstract：:The evolution of sexual reproduction remains a fascinating enigma in biology. Theoretically, populations of sexual organisms investing half of their resources into producing male offspring that don't contribute to reproduction should grow at only half the rate of their asexual counterparts. This demographic disadvanta...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep23982
更新日期：2016-04-01 00:00:00
abstract：:Most of the current studies on myocardial strain are mainly applied in patients with sinus rhythm because the image quality of arrhythmias obtained with conventional scanning sequences does not meet diagnostic needs. Here, we intend to assess left ventricular (LV) global myocardial strain in patients with arrhythmias ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-66315-z
更新日期：2020-06-09 00:00:00
abstract：:We have demonstrated a proof-of-principle experiment of reference-frame-independent phase coding quantum key distribution (RFI-QKD) over an 80-km optical fiber. After considering the finite-key bound, we still achieve a distance of 50 km. In this scenario, the phases of the basis states are related by a slowly time-va...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep03617
更新日期：2014-01-09 00:00:00
abstract：:Large bowel preparation may cause a substantial change in the gut microbiota and metabolites. Here, we included a bowel prep group and a no-procedure control group and evaluated the effects of bowel prep on the stability of the gut microbiome and metabolome as well as on recovery. Gut microbiota and metabolome composi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-40182-9
更新日期：2019-03-11 00:00:00
abstract：:The insect membrane-protein, Torso, is a member of the receptor-tyrosine-kinase family, and is activated by its ligand, prothoracicotropic hormone (PTTH). Although PTTH is one of the most important regulators of insect development, the mechanism of Torso activation by the hormone has remained elusive. In this study, u...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep22437
更新日期：2016-03-01 00:00:00
abstract：:In situ HfB2-SiC-VSi2 composite was fabricated by reactive pressureless sintering at the temperature of 2150 °C for 4 h under a vacuum atmosphere. In situ SiC and VSi2 reinforcements were formed using VC and Si powders as starting materials according to the following reaction: VC + 3Si = SiC + VSi2. Microstructural st...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-73295-7
更新日期：2020-10-06 00:00:00
abstract：:During cryopreservation, ice recrystallization is a major cause of cellular damage. Conventional cryoprotectants such as dimethyl sulfoxide (DMSO) and glycerol function by a number of different mechanisms but do not mitigate or control ice recrystallization at concentrations utilized in cryopreservation procedures. In...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep23619
更新日期：2016-03-29 00:00:00
abstract：:Polycrystalline CeGe is investigated by means of DC and AC susceptibility, non-linear DC susceptibility, electrical transport and heat capacity measurements in the low temperature regime. This compound shows two peaks at low magnetic field around TI ~ 10.7 and TII ~ 7.3 K due to antiferromagnetic ordering and subseque...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-41565-8
更新日期：2019-03-26 00:00:00
abstract：:Atmosphere-ocean coupling effect on the frequency distribution of tropical cyclones (TCs) and its future change is studied using an atmosphere and ocean coupled general circulation model (AOGCM). In the present climate simulation, the atmosphere-ocean coupling in the AOGCM improves biases in the AGCM such as the polew...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep29800
更新日期：2016-07-15 00:00:00
abstract：:Intracellular pathogens belonging to the genus Leishmania have developed effective strategies that enable them to survive within host immune cells. Immunostimulatory compounds that counteract such immunological escape mechanisms represent promising treatment options for diseases. Here, we demonstrate that a lipopeptid...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-09894-8
更新日期：2017-08-25 00:00:00
abstract：:Magnesium (Mg) is a lightweight metal with applications in transportation and sustainable battery technologies, but its current production through ore reduction using the conventional Pidgeon process emits large amounts of CO2 and particulate matter (PM2.5). In this work, a novel Pidgeon process driven by microwaves h...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep46512
更新日期：2017-04-12 00:00:00
abstract：:Albuminuria is widely used to indicate early phases of diabetic nephropathy although it is limited by the fact that structural damage might precede albumin excretion. This necessitates identifying better biomarkers that diagnose or predict diabetic nephropathy. This is a cross-sectional hospital based study recruiting...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-02421-9
更新日期：2017-06-02 00:00:00
abstract：:Understanding and manipulating the dynamic properties of the magnetic vortices stabilized in patterned ferromagnetic structures are of great interest owing to the superior resonant features with the high thermal stability and their flexible tunability. So far, numerous methods for investigating the dynamic properties ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep17922
更新日期：2015-12-09 00:00:00
abstract：:Our study is major to establish and validate a simple type||diabetes mellitus (T2DM) screening model for identifying high-risk individuals among Chinese adults. A total of 643,439 subjects who participated in the national health examination had been enrolled in this cross-sectional study. After excluding subjects with...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-68383-7
更新日期：2020-07-14 00:00:00
abstract：:Empathetic verbal feedback from others has been shown to alleviate the intensity of experimental pain. To investigate the brain changes associated with this effect, we conducted 3T-fMRI measurements in 30 healthy subjects who received painful thermal stimuli on their left hand while overhearing empathetic, neutral or ...
journal_title：Scientific reports
pub_type： 临床试验,杂志文章
doi：10.1038/s41598-019-44879-9
更新日期：2019-06-10 00:00:00
