Abstract：
:The spinal cord and mesodermal tissues of the trunk such as the vertebral column and skeletal musculature derive from neuro-mesodermal progenitors (NMPs). Sox2, Brachyury (T), and Tbx6 have been correlated with NMP potency and lineage choice; however, their exact role and interaction in these processes have not yet been revealed. Here we present a global analysis of NMPs and their descending lineages performed on purified cells from embryonic day 8.5 wild-type and mutant embryos. We show that T, cooperatively with WNT signaling, controls the progenitor state and the switch toward the mesodermal fate. Sox2 acts antagonistically and promotes neural development. T is also involved in remodeling the chromatin for mesodermal development. Tbx6 reinforces the mesodermal fate choice, represses the progenitor state, and confers paraxial fate commitment. Our findings refine previous models and establish molecular principles underlying mammalian trunk development, comprising NMP maintenance, lineage choice, and mesoderm formation.
journal_name
Dev Celljournal_title
Developmental cellauthors
Koch F,Scholze M,Wittler L,Schifferl D,Sudheer S,Grote P,Timmermann B,Macura K,Herrmann BGdoi
10.1016/j.devcel.2017.07.021subject
Has Abstractpub_date
2017-09-11 00:00:00pages
514-526.e7issue
5eissn
1534-5807issn
1878-1551pii
S1534-5807(17)30597-Xjournal_volume
42pub_type
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