Abstract:
:Telomere DNA ends with a single-stranded 3' overhang. Long 3' overhangs may cause aberrant DNA damage responses and accelerate telomere attrition, which is associated with cancer and aging, respectively. Genetic studies have indicated several important players in preventing 5' end hyper-resection, yet detailed knowledge about the molecular mechanism in which they act is still lacking. Here, we use an in vitro DNA 5' end protection assay, to study how N. castellii Cdc13 and Rap1 protect against 5' exonucleolytic degradation by λ-exonuclease. The homogeneous telomeric repeat sequence of N. castellii allows us to study their protection ability at exact binding sites relative to the 5' end. We find efficient protection by both Cdc13 and Rap1 when bound close to the 5' end. Notably, Rap1 provides protection when binding dsDNA at a distance from the 5' end. The DNA binding domain of Rap1 is sufficient for 5' end protection, and its wrapping loop region is essential. Intriguingly, Rap1 facilitates protection also when its binding site contains 2 nt of ssDNA, thus spanning across the ds-ss junction. These results highlight a role of Rap1 in 5' end protection and indicate that Cdc13 and Rap1 have complementary roles in maintaining proper 3' overhang length.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Runnberg R,Narayanan S,Cohn Mdoi
10.1038/s41598-017-08663-xsubject
Has Abstractpub_date
2017-08-18 00:00:00pages
8729issue
1issn
2045-2322pii
10.1038/s41598-017-08663-xjournal_volume
7pub_type
杂志文章abstract::Extracellular vesicles derived from mesenchymal stem cells (MSCs) represent a novel approach for regenerative and immunosuppressive therapy. Recently, cytochalasin B-induced microvesicles (CIMVs) were shown to be effective drug delivery mediators. However, little is known about their immunological properties. We propo...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-67563-9
更新日期:2020-07-01 00:00:00
abstract::Scattering of H2 on CO is of great importance in astrophysics and also is a benchmark system for comparing theory to experiment. We present here a new 6-dimensional potential energy surface for the ground electronic state of H2-CO with an estimated uncertainty of about 0.6 cm(-1) in the global minimum region, several ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep28449
更新日期:2016-06-23 00:00:00
abstract::Milk is a major food of global economic importance, and its consumption is regarded as a classic example of gene-culture evolution. Humans have exploited animal milk as a food resource for at least 8500 years, but the origins, spread, and scale of dairying remain poorly understood. Indirect lines of evidence, such as ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep07104
更新日期:2014-11-27 00:00:00
abstract::Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease for which the pathophysiological mechanisms of motor neuron loss are not precisely clarified. Environmental and epigenetic mechanisms such as microRNAs (miRNAs) could have a role in disease progression. We studied the expression pattern of miRNAs in AL...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-61246-1
更新日期:2020-03-09 00:00:00
abstract::Clinical and experimental studies indicate that muscarinic acetylcholine receptors are potential pharmacological targets for the treatment of neurological diseases. Although these receptors have been described in human, bovine and rat cerebral microvascular tissue, a subtype functional characterization in mouse brain ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-05384-z
更新日期:2017-07-11 00:00:00
abstract::Combined neutron scattering and diffusion nuclear magnetic resonance experiments have been used to reveal significant interregional asymmetries (lateralization) in bovine brain hemispheres in terms of myelin arrangement and water dynamics at micron to atomic scales. Thicker myelin sheaths were found in the left hemisp...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-51022-1
更新日期:2019-10-11 00:00:00
abstract::To increase the generation efficiency of the terahertz wave in the Y band, the idea of dual-reflector is introduced in the relativistic surface wave oscillator (SWO) with large oversized structures. The dual-reflector and the slow-wave structure (SWS) construct a resonator where the field strength of TM01 mode inside ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-55525-9
更新日期:2020-01-15 00:00:00
abstract::Neuroprotective strategies in the treatment of stroke have been attracting a great deal of attentions. Our previous clinical and basic studies have demonstrated that protopanaxadiol ginsenoside-Rd (Rd), a monomer compound extracted from Panax ginseng or Panax notoginseng, has neuroprotective effects against ischemic s...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-64738-2
更新日期:2020-05-15 00:00:00
abstract::Defect engineering is a promising route for controlling the electronic properties of monolayer transition-metal dichalcogenide (TMD) materials. Here, we demonstrate that the electronic structure of MoS2 depends sensitively on the defect charge, both its sign and magnitude. In particular, we study shallow bound states ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-31941-1
更新日期:2018-09-11 00:00:00
abstract::Thermoelectric properties of Graphene nano-ribbons (GNRs) with nanopores (NPs) are explored for a range of pore dimensions in order to achieve a high performance two-dimensional nano-scale thermoelectric device. We reduce thermal conductivity of GNRs by introducing pores in them in order to enhance their thermoelectri...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep11297
更新日期:2015-06-17 00:00:00
abstract::Many neurological disorders of gluten-related diseases (GRD), not directly referable to the gastrointestinal tract, have been reported in association with celiac disease (CD), including ataxia, neuropathy and epilepsy. In particular, people with epilepsy diagnosed with CD seems to be characterized by intractable seizu...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-14845-4
更新日期:2017-11-09 00:00:00
abstract::Many efforts have been made in the last 30 years to develop more relevant in vitro models to study genotoxic responses of drugs and environmental contaminants. While 2D HepaRG cells are one of the most promising models for liver toxicology, a switch to 3D cultures that integrate both in vivo architecture and cell-cell...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-47114-7
更新日期:2019-07-22 00:00:00
abstract::Viral oncoproteins E6/E7 play key oncogenic role in human papillomavirus (HPV)-mediated cervical carcinogenesis in conjunction with aberrant activation of cellular signaling events. GLI-signaling has been implicated in metastasis and tumor recurrence of cervical cancer. However, the interaction of GLI-signaling with H...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep34377
更新日期:2016-09-28 00:00:00
abstract::Hydrogels have been developed and applied to various biomedical applications due to their biocompatibility. However, understanding of modulation between cells to hydrogel interface is still unclear, and parameters to explain the interaction are not sophisticated enough. In this report, we studied the effect of polymer...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-38951-7
更新日期:2019-02-21 00:00:00
abstract::Al substitution in hematite is ubiquitous in soils. With the increase of Al amount, the hematite morphology changes from rhombohedral crystals to disk-shaped ones, but the underlying mechanism is poorly understood. Herein, a series of Al-substituted hematite were synthesized and characterized by synchrotron X-ray diff...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep35960
更新日期:2016-10-27 00:00:00
abstract::A direct, simultaneous calculation of properties of a liquid using an ab initio electron-correlated theory has long been unthinkable. Here we present structural, dynamical, and response properties of liquid water calculated by ab initio molecular dynamics using the embedded-fragment spin-component-scaled second-order ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep14358
更新日期:2015-09-24 00:00:00
abstract::Pachychoroid spectrum diseases have attracted increasing attention, though their pathophysiology has yet to be fully elucidated. In this study, we assessed the vascular diameters of vortex veins in pachychoroid spectrum diseases such as central serous chorioretinopathy (CSC), pachychoroid neovasculopathy without polyp...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-75789-w
更新日期:2020-11-11 00:00:00
abstract::Sirtuin 1 (SIRT1) is involved in both aging and circadian-clock regulation, yet the link between the two processes in relation to SIRT1 function is not clear. Using Sirt1-deficient mice, we found that Sirt1 and Period 2 (Per2) constitute a reciprocal negative regulation loop that plays important roles in modulating he...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep28633
更新日期:2016-06-27 00:00:00
abstract::This study investigated the effects of 4 aeration levels (varied by injection of air to the soil through subsurface irrigation lines) at two subsurface irrigation line depths (15 and 40 cm) on plant growth, yield and nutritional quality of greenhouse tomato. In all experiments, fruit number, width and length, yield, v...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep39307
更新日期:2016-12-20 00:00:00
abstract::We present results from first-principles calculations on silane (SiH4) under pressure. We find that a three dimensional P-3 structure becomes the most stable phase above 241 GPa. A prominent structural feature, which separates the P-3 structure from previously observed/predicted SiH4 structures, is that a fraction of ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep13039
更新日期:2015-08-12 00:00:00
abstract::Genotyping-by-sequencing (GBS) or restriction-site associated DNA marker sequencing (RAD-seq) is a practical and cost-effective method for analysing large genomes from high diversity species. This method of sequencing, coupled with methylation-sensitive enzymes (often referred to as methylation-sensitive restriction e...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-19655-w
更新日期:2018-02-01 00:00:00
abstract::This study sought to compare the image quality and radiation dose of coronary computed tomography angiography (CCTA) from prospectively triggered 128-slice CT (128-MSCT) versus dual-source 64-slice CT (DSCT). The study was approved by the Medical Ethics Committee at Tongji Medical College of Huazhong University of Sci...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep34795
更新日期:2016-10-18 00:00:00
abstract::Following facial nerve axotomy, nerve function is not fully restored even after reconstruction. This may be attributed to axon degeneration/neuronal death and sustained neuroinflammation. CD38 is an enzyme that catalyses the hydrolysis of nicotinamide adenine dinucleotide (NAD+) and is a candidate molecule for regulat...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-73984-3
更新日期:2020-10-20 00:00:00
abstract::Aluminium nitride (AlN) is a promising semiconductor material for use as a substrate in high-power, high-frequency electronic and deep-ultraviolet optoelectronic devices. We study the feasibility of a novel AlN fabrication technique by using the Al/GaN substitution reaction method. The substitution method we propose h...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-69992-y
更新日期:2020-08-03 00:00:00
abstract::We report on the optical and structural characterization of rubrene polycrystalline films fabricated from vacuum deposition with various substrate temperatures (Tsub). Depending on Tsub, the role of twisted and planar rubrene conformational isomers on the properties of rubrene films is focused. The temperature (T)-dep...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep40824
更新日期:2017-01-16 00:00:00
abstract::Rice bacterial blight caused by Xanthomonas oryzae pv. oryzae (Xoo) is one of the most destructive rice diseases worldwide. Therefore, in addition to breeding disease-resistant rice cultivars, it is desirable to develop effective biocontrol agents against Xoo. Here, we report that a soil bacterium Pseudomonas taiwanen...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep32950
更新日期:2016-09-08 00:00:00
abstract::The present study evaluated the physiological, molecular and hormonal parameters from scion/rootstock interaction of citrus plants during recurrent water deficit. Responses of the Valencia (VO) scion variety grafted on two rootstocks with different soil water extraction capacities, Rangpur Lime (RL) and Sunki Maravilh...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-14161-x
更新日期:2017-10-20 00:00:00
abstract::To master linguistic communication, humans must acquire large vocabularies quickly and effortlessly. Efficient word learning might be facilitated by the ability to rapidly acquire novel word forms even outside the focus of attention, occurring within minutes of repetitive exposure and suggesting fast and automatic lex...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-34029-y
更新日期:2018-10-25 00:00:00
abstract::Negative diagonal magneto-conductivity/resistivity is a spectacular- and thought provoking-property of driven, far-from-equilibrium, low dimensional electronic systems. The physical response of this exotic electronic state is not yet fully understood since it is rarely encountered in experiment. The microwave-radiatio...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep03478
更新日期:2013-12-11 00:00:00
abstract::Studies suggest that chromium deficiency is associated with elevated levels of fasting blood glucose, circulating insulin, cholesterol and triglycerides, and decreased proportion of lean body mass. However, data directly relating chromium levels to metabolic syndrome (MetS) risk are lacking. A total of 3,648 American ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep15606
更新日期:2015-10-22 00:00:00