Abstract：
:Compared with the commercially available single nucleotide polymorphism (SNP) chip based on the Bead Chip technology, the solution hybrid selection (SHS)-based target enrichment SNP chip is not only design-flexible, but also cost-effective for genotype sequencing. In this study, we propose to design an animal SNP chip using the SHS-based target enrichment strategy for the first time. As an update to the international collaboration on goat research, a 66 K SNP chip for cashmere goat was created from the whole-genome sequencing data of 73 individuals. Verification of this 66 K SNP chip with the whole-genome sequencing data of 436 cashmere goats showed that the SNP call rates was between 95.3% and 99.8%. The average sequencing depth for target SNPs were 40X. The capture regions were shown to be 200 bp that flank target SNPs. This chip was further tested in a genome-wide association analysis of cashmere fineness (fiber diameter). Several top hit loci were found marginally associated with signaling pathways involved in hair growth. These results demonstrate that the 66 K SNP chip is a useful tool in the genomic analyses of cashmere goats. The successful chip design shows that the SHS-based target enrichment strategy could be applied to SNP chip design in other species.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Qiao X,Su R,Wang Y,Wang R,Yang T,Li X,Chen W,He S,Jiang Y,Xu Q,Wan W,Zhang Y,Zhang W,Chen J,Liu B,Liu X,Fan Y,Chen D,Jiang H,Fang D,Liu Z,Wang X,Zhang Y,Mao D,Wang Z,Di R,Zhao Q,Zhong T,Yang H,Wadoi
10.1038/s41598-017-09285-zsubject
Has Abstractpub_date
2017-08-17 00:00:00pages
8621issue
1issn
2045-2322pii
10.1038/s41598-017-09285-zjournal_volume
7pub_type
杂志文章abstract：:Sex hormones may have a role in the pathophysiology of substance use disorders, as demonstrated by the association between testosterone and addictive behaviour in opioid dependence. Although opioid use has been found to suppress testosterone levels in men and women, the extent of this effect and how it relates to meth...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep06189
更新日期：2014-08-26 00:00:00
abstract：:Bacterial pneumonia is a major cause of acute lung injury and acute respiratory distress syndrome, characterized by alveolar barrier disruption. NLRP3 is best known for its ability to form inflammasomes and to regulate IL-1β and IL-18 production in myeloid cells. Here we show that NLRP3 protects the integrity of the a...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep30943
更新日期：2016-08-01 00:00:00
abstract：:The diversity of DNA sequencing methods and algorithms for genome assemblies presents scientists with a bewildering array of choices. Here, we construct and compare eight candidate assemblies combining overlapping shotgun read data, mate-pair and Chicago libraries and four different genome assemblers to produce a high...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-19430-x
更新日期：2018-01-24 00:00:00
abstract：:The American mink (Neovison vison) is a semiaquatic species of mustelid native to North America. It's an important animal for the fur industry. Many efforts have been made to locate genes influencing fur quality and color, but this search has been impeded by the lack of a reference genome. Here we present the first dr...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-15169-z
更新日期：2017-11-06 00:00:00
abstract：:Chinese olive contains plenty of polyphenols, which possess a wide range of biological actions. In this study, we aimed to investigate the role of the ethyl acetate fraction of Chinese olive fruit extract (CO-EtOAc) in the modulation of lipid accumulation in vitro and in vivo. In cellular studies, CO-EtOAc attenuated ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-19553-1
更新日期：2018-01-18 00:00:00
abstract：:Surface enhanced Raman scattering (SERS) is generally and widely used to enhance the vibrational fingerprint of molecules located at the vicinity of noble metal nanoparticles. In this work, SERS is originally used to enhance the own vibrational density of states (VDOS) of nude and isolated gold nanoparticles. This off...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep39164
更新日期：2016-12-16 00:00:00
abstract：:Enhancing genetic resistance of chickens to Newcastle Disease Virus (NDV) provides a promising way to improve poultry health, and to alleviate poverty and food insecurity in developing countries. In this study, two inbred chicken lines with different responses to NDV, Fayoumi and Leghorn, were challenged with LaSota N...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-19754-8
更新日期：2018-01-19 00:00:00
abstract：:A light reflection device combined with visible light spectrophotometer was used to measure the reflectance-wavelength curves of colored stainless steel after coloring, hardening and sealing. After these processes, the coordinate in CIE 1931 color coordinate system and brightness of samples were confirmed by chromatom...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-70359-6
更新日期：2020-08-11 00:00:00
abstract：:The G protein-coupled receptor opsin is a phospholipid scramblase that facilitates rapid transbilayer phospholipid exchange in liposomes. The mechanism by which opsin scrambles lipids is unknown. It has been proposed that lipid translocation may occur at protein-protein interfaces of opsin dimers. To test this possibi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-16842-z
更新日期：2017-12-01 00:00:00
abstract：:Transfer printing, a two-step process (i.e. picking up and printing) for heterogeneous integration, has been widely exploited for the fabrication of functional electronics system. To ensure a reliable process, strong adhesion for picking up and weak or no adhesion for printing are required. However, it is challenging ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep16133
更新日期：2015-11-10 00:00:00
abstract：:In this study, an interval optimization model is developed to maximize the benefits of a water rights transfer system that comprises industry and agriculture sectors in the Ningxia Hui Autonomous Region in China. The model is subjected to a number of constraints including water saving potential from agriculture and ec...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-02734-9
更新日期：2017-06-14 00:00:00
abstract：:The Middle East is one of the most water stressed regions in the world, receiving the majority of its hydrological input during the winter, in the form of highly variable and scattered precipitation. The persistence of wintertime anticyclonic conditions over the region can deflect storm tracks and result in extended s...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-12430-3
更新日期：2017-09-26 00:00:00
abstract：:Bacterial periplasmic-binding proteins have been acclaimed as general biosensing platform, but their range of natural ligands is too limited for optimal development of chemical compound detection. Computational redesign of the ligand-binding pocket of periplasmic-binding proteins may yield variants with new properties...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-53507-5
更新日期：2019-11-15 00:00:00
abstract：:Insects' exoskeleton, gut, hemocoel, and cells are colonized by various microorganisms that often play important roles in their host life. Moreover, insects are frequently infected by vertically transmitted symbionts that can manipulate their reproduction. The aims of this study were the characterization of bacterial ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-32747-x
更新日期：2018-09-26 00:00:00
abstract：:CRISPR/Cas9-based gene knockout in animal cells, particularly in teleosts, has proven to be very efficient with regards to mutation rates, but the precise insertion of exogenous DNA or gene knock-in via the homology-directed repair (HDR) pathway has seldom been achieved outside of the model organisms. Here, we succeed...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-79409-5
更新日期：2020-12-17 00:00:00
abstract：:The accurate prediction of the impact of an amino acid substitution on the thermal stability of a protein is a central issue in protein science, and is of key relevance for the rational optimization of various bioprocesses that use enzymes in unusual conditions. Here we present one of the first computational tools to ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep23257
更新日期：2016-03-18 00:00:00
abstract：:Immune checkpoint inhibitors (ICIs) elicit antitumour effects by activating the host immunity and cause immune-related adverse events (irAEs). ICI-related interstitial lung disease (ICI-ILD) is a fatal irAE that is difficult to treat; moreover, its incidence is relatively higher in patients with lung cancer. Therefore...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-70743-2
更新日期：2020-08-13 00:00:00
abstract：:With about 350 million people chronically infected around the world hepatitis B is a major health problem. Template for progeny HBV synthesis is the viral genome, organized as a minichromosome (cccDNA) inside the hepatocyte nucleus. How viral cccDNA gene expression is regulated by its chromatin structure; more importa...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep25901
更新日期：2016-05-13 00:00:00
abstract：:For the application of formic acid as a liquid organic hydrogen carrier, development of efficient catalysts for dehydrogenation of formic acid is a challenging topic, and most studies have so far focused on the composition of metals and supports, the size effect of metal nanoparticles, and surface chemistry of support...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep33502
更新日期：2016-09-26 00:00:00
abstract：:A correction to this article has been published and is linked from the HTML version of this paper. The error has been fixed in the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
doi：10.1038/s41598-017-14857-0
更新日期：2017-11-16 00:00:00
abstract：:Mullite nanowhiskers are prepared by a facile technique at low temperature using mica and AlF3 as raw material. Mica acts as reactant as well as substrate. By controlling the reaction temperature and holding time, the mullite nanowhisker array with uniform morphology is obtained. The nanowhisker array possesses Al-ric...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep09690
更新日期：2015-04-13 00:00:00
abstract：:The vascular smooth muscle cells (VSMCs) are exposed to interstitial flow induced shear stress that may be sensed by the surface glycocalyx, a surface layer composed primarily of proteoglycans and glycoproteins, to mediate cell contraction during the myogenic response. We, therefore, attempted to elucidate the signal ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep42092
更新日期：2017-02-13 00:00:00
abstract：:In cloud water, microorganisms are exposed to very strong stresses especially related to the presence of reactive oxygen species including H2O2 and radicals, which are the driving force of cloud chemistry. In order to understand how the bacterium Pseudomonas graminis isolated from cloud water respond to this oxidative...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-49319-2
更新日期：2019-09-05 00:00:00
abstract：:Spiny mice of the genus Acomys display several unique physiological traits, including menstruation and scar-free wound healing; characteristics that are exceedingly rare in mammals, and of considerable interest to the scientific community. These unique attributes, and the potential for spiny mice to accurately model h...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-09334-7
更新日期：2017-08-21 00:00:00
abstract：:Degradation of the biologically potent octapeptide angiotensin Ang II-(1-8) is mediated by the activities of several peptidases. The conversion of Ang II to the septapeptide Ang-(1-7) is of particular interest as the latter also confers organ protection. The conversion is catalyzed by angiotensin-converting enzyme 2 a...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep45473
更新日期：2017-04-05 00:00:00
abstract：:Inflammation is considered as a major contributor to brain injury following cerebral ischemia. The therapeutic potential of both MLC601/MLC901, which are herbal extract preparations derived from Chinese Medicine, has been reported both in advanced stroke clinical trials and also in animal and cellular models. The aim ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-36138-0
更新日期：2018-12-24 00:00:00
abstract：:We present a new general facile strategy for the preparation of protein-functionalized QDs in a single step at ambient conditions. We demonstrated that highly luminescent red to near-infrared (NIR) protein-functionalized QDs could be synthesized at room temperature in one second through a one-pot reaction that proceed...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep02825
更新日期：2013-10-02 00:00:00
abstract：:Long-term monitoring of host-parasite interactions is important for understanding the consequences of infection on host fitness and population dynamics. In an eight-year survey of the loggerhead sea turtle (Caretta caretta) population nesting in Cabo Verde, we determined the spatiotemporal variation of Ozobranchus mar...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-75498-4
更新日期：2020-10-29 00:00:00
abstract：:Sauropodomorpha were herbivorous saurischian dinosaurs that incorporate Sauropoda and early-diverging sauropodomorphs. The oldest sauropodomorph remains are known from Late Triassic deposits, most of them Gondwanan. The Laurasian record comprises some Triassic forms, but the bulk is Jurassic in age. Among the 14 Juras...
journal_title：Scientific reports
pub_type： 历史文章,杂志文章
doi：10.1038/s41598-020-67754-4
更新日期：2020-07-03 00:00:00
abstract：:Cellulose is the major component of cell wall materials. A 300 bp specific fragment from the cDNA fragment was chosen to insert into vector pFGC1008 at forward and reverse orientations to construct the recombinant RNAi vector. Knockdown of BoiCesA caused "dwarf" phenotype with smaller leaves and a loss of the content ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep41397
更新日期：2017-02-07 00:00:00
