Abstract：
:Multi-modal three dimensional (3D) optical imaging combining both structural sensitivity and molecular specificity is highly desirable in biomedical research. In this paper, we present a method termed oblique scanning laser microscopy (OSLM) to combine optical coherence tomography (OCT), for simultaneously volumetric structural and molecular imaging with cellular resolution in all three dimensions. Conventional 3D laser scanning fluorescence microscopy requires repeated optical sectioning to create z-stacks in depth. Here, the use of an obliquely scanning laser eliminates the z-stacking process, then allows highly efficient 3D OCT and fluorescence imaging by using only one raster scan. The current setup provides ~3.6 × 4.2 × 6.5 μm resolution in fluorescence imaging, ~7 × 7 × 3.5 μm in OCT in three dimensions, and the current speed of imaging is up to 100 frames per second (fps) over a volume about 0.8 × 1 × 0.5 mm3. We demonstrate several mechanisms for molecular imaging, including intrinsically expressed GFP fluorescence, autofluorescence from Flavin proteins, and exogenous antibody-conjugated dyes. We also demonstrate potential applications in imaging human intestinal organoids (HIOs), colon mucosa, and retina.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Zhang L,Capilla A,Song W,Mostoslavsky G,Yi Jdoi
10.1038/s41598-017-08822-0subject
Has Abstractpub_date
2017-08-17 00:00:00pages
8591issue
1issn
2045-2322pii
10.1038/s41598-017-08822-0journal_volume
7pub_type
杂志文章abstract：:The ovules and egg cells are well developed to be fertilized at anthesis in many flowering plants. However, ovule development is triggered by pollination in most orchids. In this study, we characterized the function of a Bsister gene, named PeMADS28, isolated from Phalaenopsis equestris, the genome-sequenced orchid. S...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-79877-9
更新日期：2021-01-13 00:00:00
abstract：:Breast cancer susceptibility gene 1 (BRCA1) located at chromosome 17q12-21 is a classic tumor suppressor gene, and has been considered as a significant role in hereditary breast cancers. Moreover, numerous studies demonstrated the methylation status of CpG islands in the promoter regions of BRCA1 gene was aberrant in ...
journal_title：Scientific reports
pub_type： 杂志文章,meta分析
doi：10.1038/srep17869
更新日期：2015-12-08 00:00:00
abstract：:Metabolomics studies of diseases associated with chronic alcohol consumption provide compelling evidence of several perturbed metabolic pathways. Moreover, the holistic approach of such studies gives insights into the pathophysiological risk factors associated with chronic alcohol-induced disability, morbidity and mor...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-24128-1
更新日期：2018-04-10 00:00:00
abstract：:Resistance to adjuvant systemic treatment, including taxanes (docetaxel and paclitaxel) is a major clinical problem for breast cancer patients. lncRNAs (long non-coding RNAs) are non-coding transcripts, which have recently emerged as important players in a variety of biological processes, including cancer development ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-36231-4
更新日期：2018-12-19 00:00:00
abstract：:BK virus (BKV) is a polyomavirus that cause of allograft dysfunction among kidney transplant recipients. The role of BKV infection in non-renal solid organ transplant recipients is not well understood neither for the relationship between various BKV strains with occurrence of BKV viral viruria. This study aimed to und...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep28491
更新日期：2016-06-24 00:00:00
abstract：:Applications for high resolution 3D profiles, so-called grayscale lithography, exist in diverse fields such as optics, nanofluidics and tribology. All of them require the fabrication of patterns with reliable absolute patterning depth independent of the substrate location and target materials. Here we present a comple...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-16496-x
更新日期：2017-11-28 00:00:00
abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
doi：10.1038/s41598-020-62443-8
更新日期：2020-03-24 00:00:00
abstract：:Inversely tapered silicon photonic resonators on silicon substrates were shown to host multiple high-Q whispering gallery modes and constitute versatile building blocks for CMOS compatible solid state lighting, optical sensing and modulator devices. So far, numerical analyses by the finite difference time domain metho...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-45034-0
更新日期：2019-06-21 00:00:00
abstract：:Electron- or X-ray-induced characteristic X-ray analysis has been widely used to determine chemical compositions of materials in vast research fields. In recent years, analysis of characteristic X-rays from muonic atoms, in which a muon is captured, has attracted attention because both a muon beam and a muon-induced c...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-15719-5
更新日期：2017-11-13 00:00:00
abstract：:The stress reactivity hypothesis posits that the magnitude of cardiovascular reactions to acute stress tasks is related with future blood pressure status, heart hypertrophy, and atherosclerosis. We assessed the stress reactivity hypothesis and aimed to identify which physiological indices (blood pressure, heart-rate, ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep06882
更新日期：2014-11-13 00:00:00
abstract：:The goal of this study was to evaluate the risk of developing acute myocardial infarction (AMI) following retinal vein occlusion (RVO). A retrospective cohort study was performed from the National Health Insurance Service and comprised 1,025,340 random subjects who were followed from 2002 to 2013. Patients with RVO in...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep22351
更新日期：2016-02-29 00:00:00
abstract：:The present study aimed to determine the effect of thyroid hormone dysfunction on brown adipose tissue activity and white adipose tissue browning in mice. Twenty randomized female C57BL/6NTac mice per treatment group housed at room temperature were rendered hypothyroid or hyperthyroid. In-vivo small animal 18F-FDG PET...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep38124
更新日期：2016-12-12 00:00:00
abstract：:The mechanisms of pattern formation during embryonic development remain poorly understood. Embryonic stem cells in culture self-organise to form spatial patterns of gene expression upon geometrical confinement indicating that patterning is an emergent phenomenon that results from the many interactions between the cell...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-73228-4
更新日期：2020-10-01 00:00:00
abstract：:Rangelands play a critical role in the global carbon cycle. However, the eco-physiological mechanisms associated with the effects of grazing on leaf photosynthesis and soil respiration remain poorly understood. To examine the impacts of grazing on leaf photosynthesis and soil respiration, we measured the photosyntheti...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep30087
更新日期：2016-07-25 00:00:00
abstract：:The rat is an important model for liver regeneration. However, there is no in vitro culture system that can capture the massive proliferation that can be observed after partial hepatectomy in rats. We here describe the generation of rat liver stem cell lines. Rat liver stem cells, which grow as cystic organoids, were ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep22154
更新日期：2016-02-26 00:00:00
abstract：:Structural disorder is widespread in eukaryotic proteins and is vital for their function in diverse biological processes. It is therefore highly desirable to be able to predict the degree of order and disorder from amino acid sequence. It is, however, notoriously difficult to predict the degree of local flexibility wi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-71716-1
更新日期：2020-09-08 00:00:00
abstract：:Part of the symptomatology of post-traumatic stress disorder (PTSD) are alterations in arousal and reactivity which could be related to a maladaptive increase in the automated sensory change detection system of the brain. In the current EEG study we investigated whether the brain's response to a simple auditory sensor...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-16669-8
更新日期：2017-11-29 00:00:00
abstract：:The ligand-induced dimerization of cell surface single-transmembrane receptors is essential for their activation. However, physiological molecules that inhibit their dimerization and activation have not been identified. ErbB3 dimerizes with ErbB2 upon binding of heregulin (HRG) to ErbB3, causing the ErbB2-catalyzed ty...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-10107-5
更新日期：2017-09-12 00:00:00
abstract：:Dengue virus (DENV) co-circulation in Brazil represents a challenge for treatment and vaccine development. Despite public health impact, the occurrence of coinfections with other viruses is a common event. Increased T cell activation and altered inflammatory response are found during DENV coinfection with Human Immuno...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-62776-4
更新日期：2020-04-14 00:00:00
abstract：:Up to 40% of congenital diseases present disturbances of brain and craniofacial development resulting in simultaneous alterations of both systems. Currently, the best available method to preclinically visualize the brain and the bones simultaneously is to co-register micro-magnetic resonance (µMR) and micro-computed t...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-75720-3
更新日期：2020-10-30 00:00:00
abstract：:Cervical oesophageal cancer (CEC) is a relatively uncommon malignancy. The biological behaviour and treatment have not been well studied. This retrospective study reviewed the clinicopathological features of 28 patients with CEC who underwent surgical resection to investigate the biological behaviour, treatment and pr...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-03593-0
更新日期：2017-06-12 00:00:00
abstract：:Much attention has been given in the literature to the effects of astrophysical events on human and land-based life. However, little has been discussed on the resilience of life itself. Here we instead explore the statistics of events that completely sterilise an Earth-like planet with planet radii in the range 0.5-1....
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-05796-x
更新日期：2017-07-14 00:00:00
abstract：:Boron carbide (B4C) coating on diamond particle is synthesized by heating diamond particles in a powder mix of H3BO3 and B in Ar atmosphere. The composition, bond state and coverage fraction of boron carbide coating on diamond particles are investigated. The boron carbide coating favors to grow on diamond (100) surfac...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-11142-y
更新日期：2017-09-06 00:00:00
abstract：:Quantum entanglement can help to increase the precision of optical phase measurements beyond the shot noise limit (SNL) to the ultimate Heisenberg limit. However, the N-photon parity measurements required to achieve this optimal sensitivity are extremely difficult to realize with current photon detection technologies,...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep02684
更新日期：2013-09-25 00:00:00
abstract：:Charge separation is a critical process for achieving high efficiencies in organic photovoltaic cells. The initial tightly bound excitonic electron-hole pair has to dissociate fast enough in order to avoid photocurrent generation and thus power conversion efficiency loss via geminate recombination. Such process takes ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-04621-9
更新日期：2017-06-27 00:00:00
abstract：:Bisphenol A (BPA) is widely used in the manufacture of plastics and epoxy resins and is prevalent in the aquatic environment. BPA disrupts endocrine pathways in fish, but the long-term developmental implications are unknown. We demonstrate that BPA deposition in the eggs of rainbow trout (Oncorhynchus mykiss), an ecol...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-13301-7
更新日期：2017-10-26 00:00:00
abstract：:The microtubule cytoskeleton is composed of α-tubulin and β-tubulin heterodimers, and it serves to regulate the shape, motility, and division of a cell. Post-translational modifications including acetylation are closely associated with the functional aspects of the microtubule, involving in a number of pathological di...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep27775
更新日期：2016-06-08 00:00:00
abstract：:The surfactant and colloidal nanoparticles has been considered for various applications because of interaction of both complex mixtures. The hydrophilic SiO2 nanoparticle could not be surface active behavior at the liquid/air interface. In this study, the SiO2 nanoparticles have been modified with 3-isocyanatopropyltr...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-76464-w
更新日期：2020-11-10 00:00:00
abstract：:Nonstoichiometric silicon nitride SiNx is a promising material for developing a new generation of high-speed, reliable flash memory device based on the resistive effect. The advantage of silicon nitride over other dielectrics is its compatibility with the silicon technology. In the present work, a silicon nitride-base...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-021-82159-7
更新日期：2021-01-28 00:00:00
abstract：:Prostate cancer (PCa) is the second most common cancer among men worldwide. Its etiology remains largely unknown compared to other common cancers. We have developed a risk stratification model combining environmental factors with family history and genetic susceptibility. 818 PCa cases and 1,006 healthy controls were ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-09386-9
更新日期：2017-08-21 00:00:00
