Abstract：
:We found that the coagulation and cytokine pathways were important mechanisms involve in the degeneration of intervertebral discs (IVD) using a microarray approach to analyze gene expression in different grades of specimens. Furthermore, using a cytokine/chemokine array, a significant increase in CXCL8 expression was observed in human nucleus pulposus (NP) cells after thrombin treatment. The enhancement of CXCL8 expression by thrombin was activated by the PAR1 receptor. Importantly, analysis of degenerated human NP tissue samples showed that EGFR expression positively correlated with the grade of tissue degeneration. In NP cells, thrombin caused an increase in phosphorylation of the EGFR at the Tyr1068, and treatment with the pharmacological EGFR inhibitor, AG1473 effectively blocked thrombin-enhanced CXCL8 production. Surprisingly, inhibition of STAT3 for 24 h decreased expression of EGFR. Treatment with thrombin also increased Akt and GSK3α/β activation; this activation was also blocked by EGFR inhibitor. Although c-Src, ERK, and FAK were activated by thrombin, only c-Src and ERK were involved in the STAT3/CXCL8 induction. Our findings indicate that stimulation of an inflammatory response in NP cells by thrombin is part of a specific pathophysiology that modulates the EGFR activation through activation of Src/ERK/STAT3 signaling.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Huang BR,Chen TS,Bau DT,Chuang IC,Tsai CF,Chang PC,Lu DYdoi
10.1038/s41598-017-09122-3subject
Has Abstractpub_date
2017-08-17 00:00:00pages
8578issue
1issn
2045-2322pii
10.1038/s41598-017-09122-3journal_volume
7pub_type
杂志文章abstract：:As critical primary producers and recyclers of organic matter, the diversity of marine protists has been extensively explored by high-throughput barcode sequencing. However, classification of short metabarcoding sequences into traditional taxonomic units is not trivial, especially for lineages mainly known by their ge...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-59524-z
更新日期：2020-02-13 00:00:00
abstract：:Archeological researches have proposed arguments for human mobility and long-distance trading over the Eurasia before the Silk Roads. Here we utilize biologically available strontium isotope analysis to assess the extent of pre-Silk Road population movements and cultural communications across the Asian interior. From ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep35162
更新日期：2016-10-20 00:00:00
abstract：:The lunar cycle is believed to strongly influence the vertical distribution of many oceanic taxa, with implications for the foraging behaviour of nocturnal marine predators. Most studies to date testing lunar effects on foraging have focused on predator activity at-sea, with some birds and marine mammals demonstrating...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-01312-3
更新日期：2017-05-03 00:00:00
abstract：:Decrease in blood concentration of branched-chain amino acids, especially leucine, is known to promote liver carcinogenesis in patients with chronic liver disease, but the mechanism is unclear. We herein established hepatocellular carcinoma (HCC) cells knocked out for DEPDC5 by using the CRISPR/Cas9 system, and elucid...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-18323-9
更新日期：2018-01-08 00:00:00
abstract：:E-mail has become the main carrier of spreading malicious software and been widely used for phishing, even high-level persistent threats. The e-mail accounts with high social reputation are primary targets to be attacked and utilized by attackers, suffering a lot of probing attacks for a long time. In this paper, in o...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-63191-5
更新日期：2020-04-29 00:00:00
abstract：:Common estimates of external training intensity for resistance exercise do not incorporate inter-set recovery duration, and might not reflect the overall demands of training. This study aimed to assess novel metrics of exercise density (ED) during resistance exercise, and how these related to a physiological marker of...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-05953-2
更新日期：2017-07-17 00:00:00
abstract：:In this report, a hand-held impulse-radar breast cancer detector is presented and the detectability of malignant breast tumors is demonstrated in the clinical test at Hiroshima University Hospital, Hiroshima, Japan. The core functional parts of the detector consist of 65-nm technology complementary metal-oxide-semicon...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-16617-6
更新日期：2017-11-27 00:00:00
abstract：:Here we report that PTEN contributes to DNA double-strand break (DSB) repair via homologous recombination (HR), as evidenced by (i) inhibition of HR in a reporter plasmid assay, (ii) enhanced sensitivity to mitomycin-C or olaparib and (iii) reduced RAD51 loading at IR-induced DSBs upon PTEN knockdown. No association w...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-22289-7
更新日期：2018-03-02 00:00:00
abstract：:In a haptic search task, one has to detect the presence of a target among distractors using the sense of touch. A salient target can be detected faster than a non-salient target. However, little is known about the exploration strategies that are used, especially in 3D search tasks where items are held in the hand. In ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep06254
更新日期：2014-09-03 00:00:00
abstract：:Ovarian cancer is the second most common gynaecologic malignancy and the main cause of death from gynaecologic cancer, due to late diagnosis and chemoresistance. Studies have reported the role of cysteine in cancer, by contributing for hydrogen sulphide (H2S) generation and as a precursor of glutathione (GSH). However...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-27753-y
更新日期：2018-06-22 00:00:00
abstract：:Deprivation of maternal care during early development markedly affects emotional development, but the underlying neuromolecular mechanisms are not fully understood. In a mouse model of disrupted mother-infant relationship, early weaning causes long-term impacts on pups to exhibit increased corticosterone secretion, an...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-40530-9
更新日期：2019-03-08 00:00:00
abstract：:The Simon effect refers to the acceleration of choice responses when the target position and response location are consistent compared with scenarios in which they are inconsistent, even if the target position is not relevant to the response. Here, we provide the first demonstration that the tactile Simon-like effect ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-27114-9
更新日期：2018-06-08 00:00:00
abstract：:Urban heat dome flow, which is also referred to as urban heat island circulation, is important for urban ventilation and pollutant transport between adjacent cities when the background wind is weak or absent. A "dome-shaped" profile can form at the upper boundary of the urban heat island circulation. The horizontal ex...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-09917-4
更新日期：2017-09-15 00:00:00
abstract：:Electrosprays operate in several modes depending on the flow rate and electric potential. This allows the deposition of droplets containing nanoparticles into discrete nanodot arrays to fabricate various electronic devices. In this study, seven different suspensions with varying properties were investigated. In the dr...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-05175-6
更新日期：2017-07-11 00:00:00
abstract：:Myostatin (MSTN) is a dominant inhibitor of skeletal muscle development and growth. Mutations in MSTN gene can lead to muscle hypertrophy or double-muscled (DM) phenotype in cattle, sheep, dog and human. However, there has not been reported significant muscle phenotypes in pigs in association with MSTN mutations. Pigs...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep14435
更新日期：2015-09-24 00:00:00
abstract：:Among patients with non-small cell lung cancer (NSCLC), best supportive care (BSC) is well-known to improve patient's quality of life and prolong survival. This study aimed to clarify (1) the decision-making factors of BSC alone and (2) the prognostic factors after selection of no further anticancer therapies. We retr...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-56431-w
更新日期：2019-12-27 00:00:00
abstract：:It is well known that collective migrations of oxygen vacancies in oxide is the key principle of resistance change in oxide-based resistive memory (OxRAM). The practical usefulness of OxRAM mainly arises from the fact that these oxygen vacancy migrations take place at relatively low operating voltages. The activation ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-53498-3
更新日期：2019-11-19 00:00:00
abstract：:Recent experiments have revealed that some membrane proteins aggregate to form clusters. This type of process has been proven to be dynamic and to be actively maintained by external kinetics. Additionally, this dynamic recruiting is cholesterol- and actin-dependent, suggesting that raft organization and cytoskeleton r...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep02608
更新日期：2013-01-01 00:00:00
abstract：:As an important online information retaining and processing function, working memory plays critical roles in many other cognitive functions. Several long-term factors, such as age, addiction and diseases, have been affirmed to impair working memory, but whether or how the short-term factors, like painful stimuli or em...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-04702-9
更新日期：2017-07-03 00:00:00
abstract：:Cell wall disruption is necessary to maximize lipid extraction yields in conventional species of mass-cultivated microalgae. This study investigated the effect of sonication, solvent choice and number of extractions on the lipid yield, lipid class composition and fatty acid composition of the diatom Porosira glacialis...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-79269-z
更新日期：2020-12-17 00:00:00
abstract：:Accumulating evidence suggests that neuroinflammatory processes are implicated in the initiation and progression of amyotrophic lateral sclerosis (ALS). Previous reports have demonstrated an increase in microgliosis and astrogliosis in the lumbar spinal cord of SOD1G93A transgenic mice before the onset of symptoms, a ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-53982-w
更新日期：2019-11-27 00:00:00
abstract：:Great Indian Bustard (GIB) is listed as Critically Endangered, with less than 250 individuals surviving in three fragmented populations. The species is under tremendous threat due to various anthropogenic pressures. Effective management and conservation of GIB requires a proper monitoring protocol, which we propose us...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-48193-2
更新日期：2019-08-12 00:00:00
abstract：:Grapevine (Vitis vinifera L.) is one of the classical fruits of the Old World. Among the thousands of domesticated grapevine varieties and variable wild sylvestris populations, the range of variation in pip morphology is very wide. In this study we scanned representative samples of grape pip populations, in an attempt...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-24738-9
更新日期：2018-04-25 00:00:00
abstract：:Phosphorus (P) is a non-renewable resource which may be depleted within next few decades; hence high P use efficiency is need of time. Plants have evolved an array of adaptive mechanisms to enhance external P acquisition and reprioritize internal utilization under P deficiency. Tissue specific biomass and P allocation...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-61147-3
更新日期：2020-03-09 00:00:00
abstract：:Large consortia efforts and genome-wide association studies (GWASs) have linked a number of genetic variants within the 6p21 chromosomal region to non-Hodgkin lymphoma (NHL). Complementing these efforts, we genotyped previously reported SNPs in the human leukocyte antigen (HLA) class I (rs6457327) and class II (rs9271...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep41400
更新日期：2017-01-31 00:00:00
abstract：:Although electroporation has been widely accepted as the main gene transfer tool, there is still considerable scope to improve the electroporation efficiency of exogenous DNAs into primary cells. Here, we developed a square-wave pulsing protocol using OptiMEM-GlutaMAX for highly efficient transfection of murine embryo...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-70258-w
更新日期：2020-08-07 00:00:00
abstract：:Although a slowing of electroencephalographic (EEG) activity during wakefulness and -to some extent- sleep of Alzheimer disease (AD) patients (i.e., increased slow-frequency activity) was documented, recent findings in healthy elderly show a decreased 0.6-1 Hz slow wave activity (SWA) during NREM, which was associated...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep39688
更新日期：2017-01-03 00:00:00
abstract：:The groundwater crisis in northwestern India is the result of over-exploitation of groundwater resources for irrigation. The Government of India has targeted a 20 percent improvement in irrigation groundwater use efficiency. In this perspective, and using a regional-scale calibrated and validated three-dimensional gro...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-70416-0
更新日期：2020-08-10 00:00:00
abstract：:By means of first principles calculation and experiments, a detailed mechanism is proposed to include the stages of slip, adjustment, and expansion for the HCP → FCC phase transformation with the prismatic relation of [Formula: see text] and [Formula: see text] in titanium. It is revealed that the formation of four FC...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-20257-9
更新日期：2018-01-31 00:00:00
abstract：:Stress-induced sensitization represents a process whereby prior exposure to severe stressors leaves animals or humans in a hyper-responsive state to further stressors. Indeed, this phenomenon is assumed to be the basis of certain stress-associated pathologies, including post-traumatic stress disorder and psychosis. On...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep31244
更新日期：2016-08-11 00:00:00
