Abstract:
:Recently, biological roles of extracellular vesicles (which include among others exosomes, microvesicles and apoptotic bodies) have attracted substantial attention in various fields of biomedicine. Here we investigated the impact of sustained exposure of cells to the fluoroquinolone antibiotic ciprofloxacin on the released extracellular vesicles. Ciprofloxacin is widely used in humans against bacterial infections as well as in cell cultures against Mycoplasma contamination. However, ciprofloxacin is an inducer of oxidative stress and mitochondrial dysfunction of mammalian cells. Unexpectedly, here we found that ciprofloxacin induced the release of both DNA (mitochondrial and chromosomal sequences) and DNA-binding proteins on the exofacial surfaces of small extracellular vesicles referred to in this paper as exosomes. Furthermore, a label-free optical biosensor analysis revealed DNA-dependent binding of exosomes to fibronectin. DNA release on the surface of exosomes was not affected any further by cellular activation or apoptosis induction. Our results reveal for the first time that prolonged low-dose ciprofloxacin exposure leads to the release of DNA associated with the external surface of exosomes.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Németh A,Orgovan N,Sódar BW,Osteikoetxea X,Pálóczi K,Szabó-Taylor KÉ,Vukman KV,Kittel Á,Turiák L,Wiener Z,Tóth S,Drahos L,Vékey K,Horvath R,Buzás EIdoi
10.1038/s41598-017-08392-1subject
Has Abstractpub_date
2017-08-15 00:00:00pages
8202issue
1issn
2045-2322pii
10.1038/s41598-017-08392-1journal_volume
7pub_type
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