Antibiotic-induced release of small extracellular vesicles (exosomes) with surface-associated DNA.

Abstract:

:Recently, biological roles of extracellular vesicles (which include among others exosomes, microvesicles and apoptotic bodies) have attracted substantial attention in various fields of biomedicine. Here we investigated the impact of sustained exposure of cells to the fluoroquinolone antibiotic ciprofloxacin on the released extracellular vesicles. Ciprofloxacin is widely used in humans against bacterial infections as well as in cell cultures against Mycoplasma contamination. However, ciprofloxacin is an inducer of oxidative stress and mitochondrial dysfunction of mammalian cells. Unexpectedly, here we found that ciprofloxacin induced the release of both DNA (mitochondrial and chromosomal sequences) and DNA-binding proteins on the exofacial surfaces of small extracellular vesicles referred to in this paper as exosomes. Furthermore, a label-free optical biosensor analysis revealed DNA-dependent binding of exosomes to fibronectin. DNA release on the surface of exosomes was not affected any further by cellular activation or apoptosis induction. Our results reveal for the first time that prolonged low-dose ciprofloxacin exposure leads to the release of DNA associated with the external surface of exosomes.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Németh A,Orgovan N,Sódar BW,Osteikoetxea X,Pálóczi K,Szabó-Taylor KÉ,Vukman KV,Kittel Á,Turiák L,Wiener Z,Tóth S,Drahos L,Vékey K,Horvath R,Buzás EI

doi

10.1038/s41598-017-08392-1

subject

Has Abstract

pub_date

2017-08-15 00:00:00

pages

8202

issue

1

issn

2045-2322

pii

10.1038/s41598-017-08392-1

journal_volume

7

pub_type

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