Generation and characterization of a mouse line for monitoring translation in dopaminergic neurons.

Abstract:

:We developed a mouse line targeting midbrain dopamine neurons for Translating Ribosome Affinity Purification(TRAP). Here, we briefly report on the basic characterization of this mouse line including confirmation of expression of the transgene in midbrain dopamine neurons and validation of its effectiveness in capturing mRNA from these cells. We also report a translational profile of these neurons which may be of use to investigators studying the gene expression of these cells. Finally, we have provided the line to Jackson Laboratories for distribution and use in future studies.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Dougherty JD

doi

10.1038/s41598-017-08618-2

subject

Has Abstract

pub_date

2017-08-14 00:00:00

pages

8117

issue

1

issn

2045-2322

pii

10.1038/s41598-017-08618-2

journal_volume

7

pub_type

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