Abstract:
:Melanoma is the leading cause of death from skin disease due to its propensity for metastasis. Studies have shown that integrin-mediated focal adhesion kinase (FAK) signal pathway is implicated in cell proliferation, survival and metastasis of tumor cells. Our previous results indicated that diallyl trisulfide (DATS) provided its antimelanoma activity via inducing cell cycle arrest and apoptosis. The aim of this study was to explore DATS mediated antimetastatic effect and the corresponding mechanism in human melanoma A375 cells. We found that DATS exhibited an inhibitory effect on the abilities of migration and invasion in A375 cells under noncytotoxic concentrations analyzed by wound healing assays and Matrigel invasion chamber system. DATS attenuated invasion of A375 cells with characteristic of decreased activities and protein expressions of matrix metalloproteinase-2 (MMP-2) and MMP-9. Moreover, DATS exerted an inhibitory effect on cell adhesion of A375 cells, which is in correlation with the change in integrin signaling pathway. Results of Western blotting showed that DATS decreased the levels of several integrin subunits, including α4, α5, αv, β1, β3 and β4. Subsequently, DATS induced a strong decrease in total FAK, phosphorylated FAK Tyr-397,-576, -577, and disorganized F-actin stress fibers, resulting in a nonmigratory phenotype. These results suggest that the antimetastatic potential of DATS for human melanoma cells might be due to the disruption of integrin/FAK signaling pathway.
journal_name
Environ Toxicoljournal_title
Environmental toxicologyauthors
Wang HC,Chu YL,Hsieh SC,Sheen LYdoi
10.1002/tox.22445subject
Has Abstractpub_date
2017-11-01 00:00:00pages
2352-2359issue
11eissn
1520-4081issn
1522-7278journal_volume
32pub_type
杂志文章abstract::Hypothyroxinemia induced by maternal mild iodine deficiency causes neurological deficits and impairments of brain function in offspring. Hypothyroxinemia is prevalent in developing and developed countries alike. However, the mechanism underlying these deficits remains less well known. Given that the myelin plays an im...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.21997
更新日期:2015-11-01 00:00:00
abstract::Nickel oxide nanoparticles (nano NiO) could induce hepatocyte apoptosis, while its potential mechanisms are unclear. This study aimed to explore the role of endoplasmic reticulum (ER) stress pathways in hepatocyte apoptosis induced by nano NiO. Male Wistar rats were administrated with nano NiO (0.015, 0.06, and 0.24 m...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22492
更新日期:2017-12-01 00:00:00
abstract::The risk presented by β-blockers on aquatic organisms remains uncertain, particularly given the enantiospecific differences in toxicity of chiral β-blockers. In this study, the toxicity of two β-blockers, propranolol and metoprolol, was determined. The 96-h LC50 of propranolol in the zebrafish larvae was 2.48 mg/L, wh...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.21867
更新日期:2014-12-01 00:00:00
abstract::Effect-directed analysis (EDA) has been reported to be a powerful tool for the identification of the responsible toxicants in contaminated, hazardous environmental samples. The aim of this study was to investigate whether it also is possible to use currently available EDA methodology to identify potentially relevant t...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.20135
更新日期:2005-10-01 00:00:00
abstract::Sick building syndrome (SBS) is a set of several clinically recognizable symptoms reported by occupants of a building without a clear cause. Neuropathy target esterase (NTE) is a membrane bound serine esterase and its reaction with organophosphates (OPs) can lead to OP-induced delayed neuropathy (OPIDN) and nerve axon...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.21839
更新日期:2014-10-01 00:00:00
abstract::Lake Elphinstone is a tropical inland water body in the far north of the Fitzroy Catchment in Central Queensland, Australia, and has experienced recurrent toxic cyanoprokaryote blooms since 1997. This article reports on an examination of the environmental conditions of the lake and the concurrent cyanoprokaryote speci...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.10142
更新日期:2003-12-01 00:00:00
abstract::Exposure to sublethal hexavalent chromium (as 2 and 4 mg L⁻¹ potassium dichromate for 1 and 2 months) during late preparatory to mid prespawning phase of annual reproductive cycle severely affected the pituitary-ovarian axis of a teleost Channa punctatus. Gonadosomatic index (GSI), ovarian histopathology, immunocytoch...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.20654
更新日期:2012-07-01 00:00:00
abstract::Airborne particulates, especially fine particles and bound chemical compounds, are a potential mediator of adverse health effects. In this study an analysis was done of the concentration and size distribution of air particulate matter, the content of bound polycyclic aromatic hydrocarbons (PAHs), and the biological ef...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.10054
更新日期:2002-01-01 00:00:00
abstract::Fluorescent pseudomonads were a major component [log (10) 4.2-6.1 colony-forming units mL-1] of the culturable heterotrophic gram-negative bacterioplankton observed in three Mississippi Delta oxbow lakes in this study. Pure cultures of fluorescent pseudomonads were isolated from three Mississippi Delta oxbow lakes (18...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/1522-7278(2001)16:1<9::aid-tox20>3.0.co;2-
更新日期:2001-01-01 00:00:00
abstract::Kupffer cells (KCs) are important in hepatic homeostasis and responses to xenobiotics. KCs are activated on interaction with endotoxin, releasing cytokines, and reactive oxygen species normally associated with increased gene expression, cellular growth, or hepatic injury. Ethanol-induced endotoxemia is one means of KC...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.21814
更新日期:2014-08-01 00:00:00
abstract::Smoking increases the risk of cardiovascular disorders and leads to damage caused by inflammation and oxidative stress. The actin cytoskeleton is a key player in the response to inflammatory stimuli and is an early target of cellular oxidative stress. The purpose of this study was to investigate the changes in actin c...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22133
更新日期:2016-11-01 00:00:00
abstract::Contamination of the urban aquatic environment with chemical and biological substances could have a long-term impact on human health because these substances threaten the integrity of the urban ecosystem and the availability of high-quality water for recreation and consumption. In light of this, the aim of the present...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.20067
更新日期:2004-12-01 00:00:00
abstract::Aluminum methylcyclohexylphosphinate (AMHP), calcium methylcyclohexylphosphinate (CMHP), aluminum diethylphosphinate (ADEP), and aluminum methylethylphosphinate (AMEP) are organic dialkyl phosphinates (DPs) and emerging phosphorus-based flame retardants. The broad-spectrum DPs flame retardants occupy high-end industri...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22383
更新日期:2017-05-01 00:00:00
abstract::Arsenite has been shown to induce a variety of oxidative damage in mammalian cells. However, the mechanisms underlying cellular responses to its adverse effects remain unknown. We previously showed that the level of Nrf2, a nuclear transcription factor significantly increased in arsenite-treated human bronchial epithe...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22374
更新日期:2017-08-01 00:00:00
abstract::Two phylogenetically distant types of test-systems-root tip meristems of barley (Hordeum vulgare) and human lymphocytes in vitro were used to detect genotoxicity and cytotoxicity induced by the herbicide paraquat (PQ) in the concentration range (10(-6) to 5 x 10(-4) mol/l). As an endpoint for cytotoxicity the mitotic ...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.20503
更新日期:2010-06-01 00:00:00
abstract::Polychlorinated biphenyls (PCBs) are a class of organic pollutants that have been linked to pancreatic disease. However, their role in affecting the exocrine function of pancreas and the underlying mechanism remains elusive. In the present study, male C57 mice were treated with Aroclor 1254, a commercially available P...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22079
更新日期:2016-06-01 00:00:00
abstract::Fascinating super paramagnetic uniqueness of iron oxide particles at nano-scale level make them extremely useful in the state of the art therapies, equipments, and techniques. Cobalt ferrite (CoFe2 O4 ) magnetic nanoparticles (MNPs) are extensively used in nano-based medicine and electronics, results in extensive disc...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22206
更新日期:2016-12-01 00:00:00
abstract::Methoxychlor (MXC), an organochloride insecticide, is a potent toxicant-targeting female reproductive system and known to cause follicular atresia by inducing apoptosis within granulosa cells. Oxidative stress plays a pivotal role in apoptosis; thus, this study focuses on the ameliorative action of N-acetyl cysteine (...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22221
更新日期:2017-01-01 00:00:00
abstract::Ouabain, a cardiotonic steroid, was used for the treatment of heart failure and atrial fibrillation and induces cancer cell apoptosis in many human cancer cells including human leukemia cells. However, there are no reports to show the effects on immune responses in a leukemia mouse model. In this study, WEHI-3 cell ge...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22732
更新日期:2019-05-01 00:00:00
abstract::Dibutyltin (DBT) is the degradation products of TBT, which is generally considered higher toxicity than TBT in the immune system. In order to learn more about the mechanisms of immune-toxic of DBT, we exposed zebrafish (Danio rerio) to 0, 1, 10 and 100 ng/L DBT for 8 weeks. At the end of the experiment, we determined ...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22502
更新日期:2018-01-01 00:00:00
abstract::In recent years, the protective effect of hydrogensulfide donor sodium hydrosulfide(NaHS) on multiple organs has been widely reported. The study aimed to explorethe effect of commonly used concentration of NaHS on theliver and its potential damage mechanism. Rats divided into 4 groups: control, NaHS I (1 mg/kg), II (3...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22868
更新日期:2020-03-01 00:00:00
abstract::The microbial assay for risk assessment (MARA) is an innovative system based on an array of 11 different microbial species freeze-dried in a 96-well micro-titer plate. Developed for testing the toxicity of chemicals, mixtures, and environmental samples, the assay employs species of a taxonomically diverse range. In ad...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.20419
更新日期:2008-10-01 00:00:00
abstract::To study the effects and possible mechanisms of suspected endocrine disrupting compounds (EDCs), a wide variety of assays have been developed. In this work, we generated engineered Escherichia coli biosensor strains that incorporate the ligand-binding domains (LBDs) of the β-subtype estrogen receptors (ERβ) from Solea...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.20708
更新日期:2013-04-01 00:00:00
abstract::Colorectal cancer (CRC) is one of the most common cancers and causes of cancer-related death. There are several first-line chemotherapeutic drugs used to treat CRC. Oxaliplatin (OXA) is an alkylating cytotoxic agent that is usually combined with other chemotherapeutic drugs to treat stage II and stage III CRC. However...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22544
更新日期:2018-05-01 00:00:00
abstract::The roles of long noncoding RNA (lncRNA) MACC1-AS1 have been revealed in various tumors. This work aims to explore the roles of lncRNA MACC1-AS1 in the stemness of nonsmall cell lung cancer (NSCLC) cells and the underlying mechanism. We showed that overexpression of MACC1-AS1 enhanced the stemness of NSCLC cells, whic...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22936
更新日期:2020-09-01 00:00:00
abstract::Cantharidin is one of the major compounds from mylabris and it has cytotoxic effects in many different types of human cancer cells. Previously, we found that cantharidin induced cell death through cell cycle arrest and apoptosis induction in human lung cancer NCI-H460 cells. However, cantharidin-affected DNA damage, r...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.21986
更新日期:2015-09-01 00:00:00
abstract::Lead ions (Pb(2+) ) are toxic industrial pollutants associated with chronic inflammatory diseases in humans and animals. Previously, we found that Pb(2+) ions induce COX-2 gene expression via the EGF receptor/nuclear factor-κB signal transduction pathway in epidermoid carcinoma cell line A431. In this study, to see wh...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.21976
更新日期:2015-09-01 00:00:00
abstract::Water bodies are routinely monitored for the presence of potentially toxic cyanobacteria; however, the methodology for confirming toxicity is currently complex and expensive. Here we describe the application of gene-based technology to rapidly identify cylindrospermopsin-producing cyanobacteria, specifically, Cylindro...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.10108
更新日期:2003-04-01 00:00:00
abstract::Liver is one of the major target organs of arsenic toxicity and carcinogenesis. Nuclear factor (erythroid-2 related) factor 2 (Nrf2) is a redox-sensitive transcription factor, regulating critically cellular defense responses against the toxic metallic arsenic in many cell types and tissues. This study was conducted to...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.20731
更新日期:2013-07-01 00:00:00
abstract::2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) and polychlorinated biphenyls (PCBs) are persistent organic pollutants which coexist in environment, and human are co-exposed to these chemicals. Our present study was aimed to investigate the possible enhanced nonalcoholic fatty liver disease (NAFLD) in ApoE(-/-) mice co-exp...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22006
更新日期:2015-12-01 00:00:00