Abstract:
:Copper resistance is a key virulence trait of the uropathogen Proteus mirabilis. Here we show that P. mirabilis ScsC (PmScsC) contributes to this defence mechanism by enabling swarming in the presence of copper. We also demonstrate that PmScsC is a thioredoxin-like disulfide isomerase but, unlike other characterized proteins in this family, it is trimeric. PmScsC trimerization and its active site cysteine are required for wild-type swarming activity in the presence of copper. Moreover, PmScsC exhibits unprecedented motion as a consequence of a shape-shifting motif linking the catalytic and trimerization domains. The linker accesses strand, loop and helical conformations enabling the sampling of an enormous folding landscape by the catalytic domains. Mutation of the shape-shifting motif abolishes disulfide isomerase activity, as does removal of the trimerization domain, showing that both features are essential to foldase function. More broadly, the shape-shifter peptide has the potential for 'plug and play' application in protein engineering.
journal_name
Nat Communjournal_title
Nature communicationsauthors
Furlong EJ,Lo AW,Kurth F,Premkumar L,Totsika M,Achard MES,Halili MA,Heras B,Whitten AE,Choudhury HG,Schembri MA,Martin JLdoi
10.1038/ncomms16065subject
Has Abstractpub_date
2017-07-19 00:00:00pages
16065issn
2041-1723pii
ncomms16065journal_volume
8pub_type
杂志文章abstract::Human-pluripotent-stem-cell-derived kidney cells (hPSC-KCs) have important potential for disease modelling and regeneration. Whether the hPSC-KCs can reconstitute tissue-specific phenotypes is currently unknown. Here we show that hPSC-KCs self-organize into kidney organoids that functionally recapitulate tissue-specif...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms9715
更新日期:2015-10-23 00:00:00
abstract::As influenza A viruses remain a major threat to human health worldwide, the discovery of broadly neutralizing monoclonal antibodies that recognize conserved epitopes would facilitate the development of antibody-based therapeutic strategies. Here we report that a VH4-4-encoded human mAb named 3E1 could neutralize H1 an...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms13577
更新日期:2016-12-02 00:00:00
abstract::Late-onset Alzheimer's disease (AD) can, in part, be considered a metabolic disease. Besides age, female sex and APOE ε4 genotype represent strong risk factors for AD that also give rise to large metabolic differences. We systematically investigated group-specific metabolic alterations by conducting stratified associa...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-14959-w
更新日期:2020-03-02 00:00:00
abstract::Heparin-like oligosaccharides mediate numerous important biological interactions, of which many are implicated in various diseases. Synthetic improvements are central to the development of such oligosaccharides as therapeutics and, in addition, there are no methods to elucidate the pharmacokinetics of structurally def...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms3016
更新日期:2013-01-01 00:00:00
abstract::Microbial biofilm formation on indwelling medical devices causes persistent infections that cannot be cured with conventional antibiotics. To address this unmet challenge, we engineer tunable active surface topographies with micron-sized pillars that can beat at a programmable frequency and force level in an electroma...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-16055-5
更新日期:2020-05-05 00:00:00
abstract::Maize rough dwarf disease (MRDD), caused by various species of the genus Fijivirus, threatens maize production worldwide. We previously identified a quantitative locus qMrdd1 conferring recessive resistance to one causal species, rice black-streaked dwarf virus (RBSDV). Here, we show that Rab GDP dissociation inhibito...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-14372-3
更新日期:2020-01-24 00:00:00
abstract::Understanding the microscopic elementary process of chemical reactions, especially in condensed phase, is highly desirable for improvement of efficiencies in industrial chemical processes. Here we show an approach to gaining new insights into elementary reactions in condensed phase by combining quantum chemical calcul...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms5238
更新日期:2014-06-25 00:00:00
abstract::The development of native-like HIV-1 envelope (Env) trimer antigens has enabled the induction of neutralizing antibody (NAb) responses against neutralization-resistant HIV-1 strains in animal models. However, NAb responses are relatively weak and narrow in specificity. Displaying antigens in a multivalent fashion on n...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-12080-1
更新日期:2019-09-19 00:00:00
abstract::Strain engineering is widely used in material science to tune the (opto-)electronic properties of materials and enhance the performance of devices. Two-dimensional atomic crystals are a versatile playground to study the influence of strain, as they can sustain very large deformations without breaking. Various optical ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-02830-y
更新日期:2018-02-06 00:00:00
abstract::Normalization is a common cortical computation widely observed in sensory perception, but its importance in perception of reward value and decision making remains largely unknown. We examined (1) whether normalized value signals occur in the orbitofrontal cortex (OFC) and (2) whether changes in behavioral task context...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-017-02614-w
更新日期:2018-01-11 00:00:00
abstract::Non-line-of-sight (NLOS) imaging recovers objects using diffusely reflected indirect light using transient illumination devices in combination with a computational inverse method. While capture systems capable of collecting light from the entire NLOS relay surface can be much more light efficient than single pixel poi...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-15157-4
更新日期:2020-04-02 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Nature communications
pub_type: 已发布勘误
doi:10.1038/s41467-020-15018-0
更新日期:2020-02-25 00:00:00
abstract::The Greenland ice sheet has become one of the main contributors to global sea level rise, predominantly through increased meltwater runoff. The main drivers of Greenland ice sheet runoff, however, remain poorly understood. Here we show that clouds enhance meltwater runoff by about one-third relative to clear skies, us...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms10266
更新日期:2016-01-12 00:00:00
abstract::Detecting the metallic Dirac electronic states on the surface of Topological Insulators (TIs) is critical for the study of important surface quantum properties (SQPs), such as Majorana zero modes, where simultaneous probing of the bulk and edge electron states is required. However, there is a particular shortage of ex...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-14838-4
更新日期:2020-03-09 00:00:00
abstract::Blood brain barrier (BBB) breakdown is not only a consequence of but also contributes to many neurological disorders, including stroke and Alzheimer's disease. How the basement membrane (BM) contributes to the normal functioning of the BBB remains elusive. Here we use conditional knockout mice and an acute adenovirus-...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms4413
更新日期:2014-03-03 00:00:00
abstract::The original version of this Article contained an error in the spelling of the author David Bulkley, which was incorrectly given as David Bulkey. This has now been corrected in both the PDF and HTML versions of the Article. ...
journal_title:Nature communications
pub_type: 杂志文章,已发布勘误
doi:10.1038/s41467-019-09422-4
更新日期:2019-03-26 00:00:00
abstract::Telomere maintenance is a universal hallmark of cancer. Most tumors including low-grade oligodendrogliomas use telomerase reverse transcriptase (TERT) expression for telomere maintenance while astrocytomas use the alternative lengthening of telomeres (ALT) pathway. Although TERT and ALT are hallmarks of tumor prolifer...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-20312-y
更新日期:2021-01-04 00:00:00
abstract::Global emergence of Gram-negative bacteria carrying the plasmid-borne resistance genes, blaMBL and mcr, raises a significant challenge to the treatment of life-threatening infections by the antibiotics, carbapenem and colistin (COL). Here, we identify an antirheumatic drug, auranofin (AUR) as a dual inhibitor of metal...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-18939-y
更新日期:2020-10-16 00:00:00
abstract::Coordinated activity of VEGF and Notch signals guides the endothelial cell (EC) specification into tip and stalk cells during angiogenesis. Notch activation in stalk cells leads to proliferation arrest via an unknown mechanism. By using gain- and loss-of-function gene-targeting approaches, here we show that PTEN is cr...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms8935
更新日期:2015-07-31 00:00:00
abstract::Soft particulate media include a wide range of systems involving athermal dissipative particles both in non-living and biological materials. Characterization of flows of particulate media is of great practical and theoretical importance. A fascinating feature of these systems is the existence of a critical rigidity tr...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-017-00022-8
更新日期:2017-04-10 00:00:00
abstract::Yeast lacks dedicated photoreceptors; however, blue light still causes pronounced oscillations of the transcription factor Msn2 into and out of the nucleus. Here we show that this poorly understood phenomenon is initiated by a peroxisomal oxidase, which converts light into a hydrogen peroxide (H2O2) signal that is sen...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms14791
更新日期:2017-03-24 00:00:00
abstract::Glucagon is released from pancreatic α-cells to activate pathways that raise blood glucose. Its secretion is regulated by α-cell-intrinsic glucose sensing and paracrine control through insulin and somatostatin. To understand the inadequately high glucagon levels that contribute to hyperglycemia in type-2 diabetes (T2D...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-15717-8
更新日期:2020-04-20 00:00:00
abstract::Adenovirus is a nuclear replicating DNA virus reliant on host RNA processing machinery. Processing and metabolism of cellular RNAs can be regulated by METTL3, which catalyzes the addition of N6-methyladenosine (m6A) to mRNAs. While m6A-modified adenoviral RNAs have been previously detected, the location and function o...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-19787-6
更新日期:2020-11-26 00:00:00
abstract::Rapid and, in many cases, unprecedented Arctic climate changes are having far-reaching impacts on natural and human systems. Despite state-of-the-art climate models capturing the rapid nature of Arctic climate change, termed Arctic amplification, they significantly disagree on its magnitude. Using a regional, process-...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-07061-9
更新日期:2018-11-27 00:00:00
abstract::The field of optical metrology with its high precision position, rotation and wavefront sensors represents the basis for lithography and high resolution microscopy. However, the on-chip integration-a task highly relevant for future nanotechnological devices-necessitates the reduction of the spatial footprint of sensin...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-16739-y
更新日期:2020-06-09 00:00:00
abstract::Tumour vessels have been studied extensively as they are critical sites for drug delivery, anti-angiogenic therapies and immunotherapy. As a preclinical tool, intravital microscopy (IVM) allows for in vivo real-time direct observation of vessels at the cellular level. However, to date there are no reports of intravita...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms10684
更新日期:2016-02-17 00:00:00
abstract::Exfoliated two-dimensional (2D) sheets can readily stack to form flexible, free-standing films with lamellar microstructure. The interlayer spaces in such lamellar films form a percolated network of molecularly sized, 2D nanochannels that could be used to regulate molecular transport. Here we report self-assembled cla...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms8602
更新日期:2015-07-13 00:00:00
abstract::The quiescent centre (QC) in the Arabidopsis root apical meristem is essential for stem cell organization. Here we show that the loss of REPRESSOR OF WUSCHEL1 (ROW1), a PHD domain-containing protein, leads to QC failure, defects in cell differentiation and ectopic expression of WUSCHEL-RELATED HOMEOBOX 5 (WOX5) in cel...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms7003
更新日期:2015-01-29 00:00:00
abstract::Reliable quantum information processing in the face of errors is a major fundamental and technological challenge. Quantum error correction protects quantum states by encoding a logical quantum bit (qubit) in multiple physical qubits. To be compatible with universal fault-tolerant computations, it is essential that sta...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms11526
更新日期:2016-05-05 00:00:00
abstract::Alkaline-urea aqueous solvent system provides a novel and important approach for the utilization of polysaccharide. As one of the most important polysaccharide, chitosan can be well dissolved in this solvent system, and the resultant hydrogel material possesses unique and excellent properties. Thus the sound understan...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms12033
更新日期:2016-06-23 00:00:00