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Post-termination Ribosome Intermediate Acts as the Gateway to Ribosome Recycling.

Abstract：

:During termination of translation, the nascent peptide is first released from the ribosome, which must be subsequently disassembled into subunits in a process known as ribosome recycling. In bacteria, termination and recycling are mediated by the translation factors RF, RRF, EF-G, and IF3, but their precise roles have remained unclear. Here, we use single-molecule fluorescence to track the conformation and composition of the ribosome in real time during termination and recycling. Our results show that peptide release by RF induces a rotated ribosomal conformation. RRF binds to this rotated intermediate to form the substrate for EF-G that, in turn, catalyzes GTP-dependent subunit disassembly. After the 50S subunit departs, IF3 releases the deacylated tRNA from the 30S subunit, thus preventing reassembly of the 70S ribosome. Our findings reveal the post-termination rotated state as the crucial intermediate in the transition from termination to recycling.

journal_name

Cell Rep

journal_title

Cell reports

authors

Prabhakar A,Capece MC,Petrov A,Choi J,Puglisi JD

doi

10.1016/j.celrep.2017.06.028

subject

Has Abstract

pub_date

2017-07-05 00:00:00

pages

161-172

issue

1

issn

2211-1247

pii

S2211-1247(17)30825-2

journal_volume

20

pub_type

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