Abstract:
:Recent reports have indicated human embryonic stem cells-derived midbrain dopamine (mDA) neurons as proper cell resources for use in Parkinson's disease (PD) therapy. Nevertheless, no detailed and systematic study has been conducted to identify which differentiation stages of mDA cells are most suitable for transplantation in PD therapy. Here, we transplanted three types of mDA cells, DA progenitors (differentiated in vitro for 16 days [D16]), immature DA neurons (D25), and DA neurons (D35), into PD mice and found that all three types of cells showed high viability and strong neuronal differentiation in vivo. Both D25 and D35 cells showed neuronal maturation and differentiation toward TH+ cells and, accordingly, satisfactory behavioral functional recovery. However, transplanted D16 cells were less capable of producing functional recovery. These findings provide a valuable guideline for standardizing the differentiation stage of the transplantable cells used in clinical cell therapy for PD. Stem Cells Translational Medicine 2017;6:1803-1814.
journal_name
Stem Cells Transl Medjournal_title
Stem cells translational medicineauthors
Qiu L,Liao MC,Chen AK,Wei S,Xie S,Reuveny S,Zhou ZD,Hunziker W,Tan EK,Oh SKW,Zeng Ldoi
10.1002/sctm.16-0470subject
Has Abstractpub_date
2017-09-01 00:00:00pages
1803-1814issue
9eissn
2157-6564issn
2157-6580journal_volume
6pub_type
杂志文章abstract::Osteoarthritis (OA) remains an intractable clinical challenge. Few drugs are available for reversing this degenerative disease, although some promising candidates have performed well in preclinical studies. Tumor necrosis factor α (TNFα) has been identified as a crucial effector modulating OA pathogenesis. This study ...
journal_title:Stem cells translational medicine
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pub_type: 杂志文章
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