Abstract:
:Mesenchymal stem cells (MSCs) have been used for cell-based therapies in regenerative medicine, with increasing importance in central and peripheral nervous system repair. However, MSCs grafting present disadvantages, such as, a high number of cells required for transplantation and low survival rate when transplanted into the central nervous system (CNS). In line with this, MSCs secretome which present on its composition a wide range of molecules (neurotrophins, cytokines) and microvesicles, can be a solution to surpass these problems. However, the effect of MSCs secretome in axonal elongation is poorly understood. In this study, we demonstrate that application of MSCs secretome to both rat cortical and hippocampal neurons induces an increase in axonal length. In addition, we show that this growth effect is axonal intrinsic with no contribution from the cell body. To further understand which are the molecules required for secretome-induced axonal outgrowth effect, we depleted brain-derived neurotrophic factor (BDNF) from the secretome. Our results show that in the absence of BDNF, secretome-induced axonal elongation effect is lost and that axons present a reduced axonal growth rate. Altogether, our results demonstrate that MSCs secretome is able to promote axonal outgrowth in CNS neurons and this effect is mediated by BDNF.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Martins LF,Costa RO,Pedro JR,Aguiar P,Serra SC,Teixeira FG,Sousa N,Salgado AJ,Almeida RDdoi
10.1038/s41598-017-03592-1subject
Has Abstractpub_date
2017-06-23 00:00:00pages
4153issue
1issn
2045-2322pii
10.1038/s41598-017-03592-1journal_volume
7pub_type
杂志文章abstract::Several systems can be represented as multiplex networks, i.e. in terms of a superposition of various graphs, each related to a different mode of connection between nodes. Hence, the definition of proper mathematical quantities aiming at capturing the added level of complexity of those systems is required. Various ste...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep09120
更新日期:2015-03-13 00:00:00
abstract::Wave propagation is an important characteristic for pattern formation and pattern dynamics. To date, various waves in homogeneous media have been investigated extensively and have been understood to a great extent. However, the wave behaviors in heterogeneous media have been studied and understood much less. In this w...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep25177
更新日期:2016-05-04 00:00:00
abstract::Study of knockout (KO) mice has helped understand the link between many genes/proteins and human diseases. Identification of infertile KO mice provides valuable tools to characterize the molecular mechanisms underlying gamete formation. The KIAA0319L gene has been described to have a putative association with dyslexia...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-28666-6
更新日期:2018-07-10 00:00:00
abstract::California has experienced a dry 21(st) century capped by severe drought from 2012 through 2015 prompting questions about hydroclimatic sensitivity to anthropogenic climate change and implications for the future. We address these questions using a Holocene lake sediment record of hydrologic change from the Sierra Neva...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep33325
更新日期:2016-09-15 00:00:00
abstract::We have applied a combined computational procedure based on inverse and direct docking in order to identify putative protein targets of a panel of mycotoxins and xenobiotic compounds that can contaminate food and that are known to have several detrimental effects on human health. This procedure allowed us to identify ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-15148-4
更新日期:2017-11-09 00:00:00
abstract::Inositol Requiring Enzyme-1 (IRE1) is the most conserved transducer of the Unfolded Protein Response (UPR), a surveillance mechanism that ensures homeostasis of the endoplasmic reticulum (ER) in eukaryotes. IRE1 activation orchestrates adaptive responses, including lipid anabolism, metabolic reprogramming, increases i...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-16662-1
更新日期:2017-11-28 00:00:00
abstract::Social immunity forms an essential part of the defence repertoire of social insects. In response to infestation by the parasitic mite Varroa destructor and its associated viruses, honey bees (Apis mellifera L.) have developed a specific behaviour (varroa-sensitive hygiene, or VSH) that helps protect the colony from th...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep25444
更新日期:2016-05-03 00:00:00
abstract::As the magnetic storage density increases in commercial products, e.g. the hard disc drives, a full understanding of dynamic magnetism in nanometer resolution underpins the development of next-generation products. Magnetic force microscopy (MFM) is well suited to exploring ferromagnetic domain structures. However, ato...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep05594
更新日期:2014-07-11 00:00:00
abstract::Salvia miltiorrhiza Bunge, which contains tanshinones and phenolic acids as major classes of bioactive components, is one of the most widely used herbs in traditional Chinese medicine. Production of tanshinones and phenolic acids is enhanced by methyl jasmonate (MeJA). Transcription factor MYC2 is the switch of jasmon...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep22852
更新日期:2016-03-07 00:00:00
abstract::Nitric oxide (NO), a vascular signaling molecule, is primarily produced by endothelial NO synthase. Recently, a functional endothelial NO synthase (eNOS) was described in red blood cells (RBC). The RBC-eNOS contributes to the intravascular NO pool and regulates physiological functions. However the regulatory mechanism...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep26935
更新日期:2016-06-27 00:00:00
abstract::Adulterant herbal materials are a threat to consumer safety. In this study, we used DNA barcoding to investigate the proportions and varieties of adulterant species in traditional Chinese medicine (TCM) markets. We used a DNA barcode database of TCM (TCMD) that was established by our group to investigate 1436 samples ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep18723
更新日期:2016-01-07 00:00:00
abstract::Genetic circuit-based biosensors have emerged as an effective analytical tool in synthetic biology; these biosensors can be applied to high-throughput screening of new biocatalysts and metabolic pathways. Sigma 54 (σ54)-dependent transcription factor (TF) can be a valuable component of these biosensors owing to its in...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-62892-1
更新日期:2020-04-08 00:00:00
abstract::Targeting myeloid differentiation protein 2 (MD-2) or Toll-like receptor 4 (TLR4) with small molecule inhibitor rescues the systemic inflammatory response syndrome (SIRS) in sepsis due to infection with Gram-negative bacteria but not other microbes. Herein, we provided IκB kinase β (IKKβ) in innate immune process as a...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep41180
更新日期:2017-02-01 00:00:00
abstract::Jute fibre is the second most important fibre next to cotton. It is obtained from the bark of plant through microbial retting process. Here we report optimized microbial retting protocol that can lower retting period and produce high fibre quality. A total of 451 bacterial colonies have been isolated from five jute re...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-61898-z
更新日期:2020-03-20 00:00:00
abstract::The heterogeneity of metabolic reactions leads to a non-uniform distribution of temperature in different parts of the living cell. The demand to study normal functioning and pathological abnormalities of cellular processes requires the development of new visualization methods. Previously, we have shown that the 35-kDa...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-45421-7
更新日期:2019-06-20 00:00:00
abstract::This is the first pharmacological characterisation of a neuropeptide G protein-coupled receptor (GPCR) in a crustacean. We cloned the ORF of the red pigment-concentrating hormone from a German strain of Daphnia pulex (Dappu-RPCH), as well as that of the cognate receptor (Dappu-RPCHR). Dappu-RPCHR has the hallmarks of ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-06805-9
更新日期:2017-07-31 00:00:00
abstract::We report a strategy to achieve a discrete cage molecule featuring a high level of structural hierarchy through a multiple-assembly process. A cobalt (Co) supramolecular triple-stranded helicate (Co-TSH)-based discrete molecular cage (1) is successfully synthesized and fully characterized. The solid-state structure of...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep43448
更新日期:2017-03-06 00:00:00
abstract::Nitric oxide (NO) produced by endothelial cells in response to cytokines displays anti-inflammatory activity by preventing the adherence, migration and activation of neutrophils. The molecular mechanism by which NO operates at the blood-endothelium interface to exert anti-inflammatory properties is largely unknown. He...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-16342-0
更新日期:2017-11-23 00:00:00
abstract::To address the limitations of titanium dioxide (TiO2) and expand the applicability of the photocatalytic activity of TiO2,WSe2 and silica, an eco-friendly, self-assembled method for combining a silica precursor with a WSe2-graphene-TiO2 composite with cetyltrimethylammonium bromide (CTAB) as surface active agents is p...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-31188-w
更新日期:2018-08-24 00:00:00
abstract::Bears are iconic mammals with a complex evolutionary history. Natural bear hybrids and studies of few nuclear genes indicate that gene flow among bears may be more common than expected and not limited to polar and brown bears. Here we present a genome analysis of the bear family with representatives of all living spec...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep46487
更新日期:2017-04-19 00:00:00
abstract::The functions of purinergic P2 receptors (P2Rs) for extracellular adenosine triphosphate (ATP) are poorly understood. Here, for the first time, we show that activation of P2Rs in an important arousal region, the basal forebrain (BF), promotes wakefulness, whereas inhibition of P2Rs promotes sleep. Infusion of a non-hy...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-29103-4
更新日期:2018-07-16 00:00:00
abstract::We have proposed and successfully demonstrated a novel approach to direct conversion of mechanical energy into electrical energy using microfluidics. The method combines previously demonstrated reverse electrowetting on dielectric (REWOD) phenomenon with the fast self-oscillating process of bubble growth and collapse....
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep16537
更新日期:2015-11-16 00:00:00
abstract::Materials with a layered structure have attracted tremendous attention because of their unique properties. The ultrathin nanosheet structure can result in extremely rapid intercalation/de-intercalation of Na ions in the charge-discharge progress. Herein, we report a manganese oxide with pre-intercalated K and Na ions ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-02028-0
更新日期:2017-05-22 00:00:00
abstract::Integrin α11β1 is a collagen-binding integrin, which is receiving increasing attention in the context of wound healing and fibrosis. Although α11β1 integrin displays similar collagen specificity to α2β1 integrin, both integrins have distinct in vivo functions. In this context, the contribution of α11 subunit cytoplasm...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-51689-6
更新日期:2019-10-25 00:00:00
abstract::MicroRNA (miRNA) regulates gene expression by binding to specific sites in the 3'untranslated regions of its target genes. Machine learning based miRNA target prediction algorithms first extract a set of features from potential binding sites (PBSs) in the mRNA and then train a classifier to distinguish targets from no...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep08004
更新日期:2015-01-23 00:00:00
abstract::The search for efficient chemotherapy drugs and other anti-cancer treatments would benefit from a deeper understanding of the tumor microenvironment (TME) and its role in tumor progression. Because in vivo experimental methods are unable to isolate or control individual factors of the TME and in vitro models often do ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-59658-0
更新日期:2020-02-20 00:00:00
abstract::Facial mimicry has been suggested to be a behavioral index for emotional empathy. The present study is the first to investigate the link between facial muscle activity and empathy for pain by facial electromyographic (EMG) recording while observers watched videos depicting real-life painful events. Three types of visu...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep16988
更新日期:2015-12-09 00:00:00
abstract::Exposure to stress during early life affects subsequent behaviors and increases the vulnerability to adult pathologies, a phenomenon that has been well documented in humans and rodents. In this study, we introduce a chronic unpredictable stress protocol adapted to young zebrafish, which is an increasingly popular vert...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-67182-4
更新日期:2020-06-25 00:00:00
abstract::The aim of this study was to identify the synergistic effect of microRNA expression with classical risk factors of coronary heart disease (CHD) and to explore their diagnostic value for coronary stenotic lesions in subjects with CHD. Plasma samples were obtained from 66 subjects with CHD and from 58 control individual...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep14925
更新日期:2015-10-08 00:00:00
abstract::The surveillance of infectious diseases is of great importance for disease control and prevention, and more attention should be paid to the Class B notifiable diseases in China. Meanwhile, according to the International Monetary Fund (IMF), the annual growth of Chinese gross domestic product (GDP) would decelerate bel...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-016-0020-5
更新日期:2016-12-23 00:00:00