Abstract:
BACKGROUND:The emergence of fosfomycin resistance and extended-spectrum β-lactamase (ESBL) genes is a serious threat to public health and a new challenge in shigellosis treatment. The purpose of this study was to identify fosfomycin resistance and characterize β-lactamase genes in fos-carrying isolates of Shigella flexneri from patients in China. METHODS:A total of 263 S. flexneri isolates were collected from 34 hospitals in the Anhui Province of China during September 2012-September 2015 and screened for fosA3, fosA, and fosC2 by PCR amplification and sequencing. The fos-carrying isolates were then screened for β-lactamase genes. The clonal relationships between fosA3-carrying isolates, the transmissibility of fosfomycin resistance, replicon types of plasmids carrying fosfomycin resistance genes and other associated resistance genes were investigated. RESULTS:Twenty-five of the 263 isolates (9.5%) showed resistance to fosfomycin, and 18 (6.8%) were positive for fosA3. None of the isolates was positive for fosA or fosC2. Seventeen of the isolates carrying fosA3 (94%) were CTX-M producers (seven CTX-M-55, five CTX-M-14, and five CTX-M-123), while three (16.7%) were TEM producers (TEM-1).Sixteen (88.9%) fosA3-carrying isolates exhibited multi-drug resistance. The replicon types of the 13 fosA3-carrying plasmids were IncF (n=13), IncHI2 (n=3), IncIl-Ir (n=2), and IncN (n=1). CONCLUSIONS:Our results indicated that fosA3 could spread through plasmids in S. flexneri isolates, along with the bla(CTX-M) and bla(TEM), which facilitate its quick dispersal. To the best of our knowledge, this is the first report of CTX-M-123-type ESBLs in S. flexneri isolates from patients in China.
journal_name
Ann Lab Medjournal_title
Annals of laboratory medicineauthors
Liu Y,Cheng Y,Yang H,Hu L,Cheng J,Ye Y,Li Jdoi
10.3343/alm.2017.37.5.415subject
Has Abstractpub_date
2017-09-01 00:00:00pages
415-419issue
5eissn
2234-3806issn
2234-3814pii
37.415journal_volume
37pub_type
杂志文章abstract:BACKGROUND:The gold standard for antinuclear antibody (ANA) screening is the indirect immunofluorescence (IIF) assay with human epithelial cells (HEp-2). However, a number of substantial disadvantages of manual IIF assays have highlighted the need for the automation and standardization of fluorescent ANA (FANA) testing...
journal_title:Annals of laboratory medicine
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更新日期:2015-01-01 00:00:00
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更新日期:2015-09-01 00:00:00
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journal_title:Annals of laboratory medicine
pub_type: 杂志文章
doi:10.3343/alm.2013.33.6.415
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pub_type: 杂志文章
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abstract::[This corrects the article on p. 439 in vol. 40.]. ...
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pub_type: 杂志文章,已发布勘误
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更新日期:2021-01-01 00:00:00
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journal_title:Annals of laboratory medicine
pub_type: 杂志文章,评审
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journal_title:Annals of laboratory medicine
pub_type: 杂志文章
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更新日期:2012-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:2012-03-01 00:00:00
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journal_title:Annals of laboratory medicine
pub_type: 杂志文章
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更新日期:2021-05-01 00:00:00
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journal_title:Annals of laboratory medicine
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更新日期:2017-11-01 00:00:00
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journal_title:Annals of laboratory medicine
pub_type: 杂志文章
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更新日期:2016-09-01 00:00:00