Production of a tissue-like structure by contraction of collagen lattices by human fibroblasts of different proliferative potential in vitro.

Abstract:

:Fibroblasts can condense a hydrated collagen lattice to a tissue-like structure 1/28th the area of the starting gel in 24 hr. The rate of the process can be regulated by varying the protein content of the lattice, the cell number, or the concentration of an inhibitor such as Colcemid. Fibroblasts of high population doubling level propagated in vitro, which have left the cell cycle, can carry out the contraction at least as efficiently as cycling cells. The potential uses of the system as an immunologically tolerated "tissue" for wound healing and as a model for studying fibroblast function are discussed.

authors

Bell E,Ivarsson B,Merrill C

doi

10.1073/pnas.76.3.1274

subject

Has Abstract

pub_date

1979-03-01 00:00:00

pages

1274-8

issue

3

eissn

0027-8424

issn

1091-6490

journal_volume

76

pub_type

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