Functional Evaluation of a Bioartificial Liver Support System Using Immobilized Hepatocyte Spheroids in a Porcine Model of Acute Liver Failure.

Abstract:

:Bioartificial livers (BAL) may offer acute liver failure (ALF) patients an opportunity for cure without liver transplantation. We evaluated the efficacy of a spheroid-based BAL system, containing aggregates of porcine hepatocytes, in a porcine model of ALF. ALF pigs were divided into three groups. The control group consisted of treatment naïve pigs (n = 5), blank group consisted of pigs that were attached to the BAL system not containing hepatocytes for 12 hours (n = 5) and BAL group consisted of pigs that were attached to the BAL containing hepatocytes for 12 hours (n = 5). Increase in serum ammonia levels were significantly greater in the blank group (P < 0.01) and control group (P < 0.01), compared to the BAL group during the treatment period. Increase in ICP was significantly greater in the control group compared to the BAL group (P = 0.01). Survival was significantly prolonged in the BAL group compared to the blank group (P = 0.03). A BAL system with a bioreactor containing hepatocyte spheroids showed effective clearance of serum ammonia, preservation of renal function and delayed ICP increase in a porcine model of ALF.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Lee JH,Lee DH,Lee S,Kwon CHD,Ryu JN,Noh JK,Jang IK,Park HJ,Yoon HH,Park JK,Kim YJ,Kim SK,Lee SK

doi

10.1038/s41598-017-03424-2

subject

Has Abstract

pub_date

2017-06-19 00:00:00

pages

3804

issue

1

issn

2045-2322

pii

10.1038/s41598-017-03424-2

journal_volume

7

pub_type

杂志文章
  • Ultrasonic spray coating polymer and small molecular organic film for organic light-emitting devices.

    abstract::Ultrasonic spray coating process (USCP) with high material -utilization, low manufacture costs and compatibility to streamline production has been attractive in researches on photoelectric devices. However, surface tension exists in the solvent is still a huge obstacle to realize smooth organic film for organic light ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep37042

    authors: Liu S,Zhang X,Zhang L,Xie W

    更新日期:2016-11-22 00:00:00

  • Signs of biological activities of 28,000-year-old mammoth nuclei in mouse oocytes visualized by live-cell imaging.

    abstract::The 28,000-year-old remains of a woolly mammoth, named 'Yuka', were found in Siberian permafrost. Here we recovered the less-damaged nucleus-like structures from the remains and visualised their dynamics in living mouse oocytes after nuclear transfer. Proteomic analyses demonstrated the presence of nuclear components ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-40546-1

    authors: Yamagata K,Nagai K,Miyamoto H,Anzai M,Kato H,Miyamoto K,Kurosaka S,Azuma R,Kolodeznikov II,Protopopov AV,Plotnikov VV,Kobayashi H,Kawahara-Miki R,Kono T,Uchida M,Shibata Y,Handa T,Kimura H,Hosoi Y,Mitani T,Matsumo

    更新日期:2019-03-11 00:00:00

  • Prediction of Alzheimer's disease using blood gene expression data.

    abstract::Identification of AD (Alzheimer's disease)-related genes obtained from blood samples is crucial for early AD diagnosis. We used three public datasets, ADNI, AddNeuroMed1 (ANM1), and ANM2, for this study. Five feature selection methods and five classifiers were used to curate AD-related genes and discriminate AD patien...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-60595-1

    authors: Lee T,Lee H

    更新日期:2020-02-26 00:00:00

  • Compounds from multilayer plastic bags cause reproductive failures in artificial insemination.

    abstract::High levels of reproductive failure were detected in some Spanish sow farms in the Spring of 2010. Regular returns to estrus and variable reductions in litter size were observed. The problem started suddenly and did not appear to be related to the quality of the ejaculates, disease, alterations of body condition or an...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep04913

    authors: Nerin C,Ubeda JL,Alfaro P,Dahmani Y,Aznar M,Canellas E,Ausejo R

    更新日期:2014-05-09 00:00:00

  • Geographic variation in baseline innate immune function does not follow variation in aridity along a tropical environmental gradient.

    abstract::Geographic variation in aridity determines environmental productivity patterns, including large-scale variability in pathogens, vectors and associated diseases. If disease risk decreases with increasing aridity and is matched by immune defense, we predict a decrease in innate immune function along a gradient of increa...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-62806-1

    authors: Nwaogu CJ,Cresswell W,Tieleman BI

    更新日期:2020-04-03 00:00:00

  • Fractional Solitons in Excitonic Josephson Junctions.

    abstract::The Josephson effect is especially appealing to physicists because it reveals macroscopically the quantum order and phase. In excitonic bilayers the effect is even subtler due to the counterflow of supercurrent as well as the tunneling between layers (interlayer tunneling). Here we study, in a quantum Hall bilayer, th...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep15796

    authors: Hsu YF,Su JJ

    更新日期:2015-10-29 00:00:00

  • Magnetically-induced ferroelectricity in the (ND4)2[FeCl5(D2O)] molecular compound.

    abstract::The number of magnetoelectric multiferroic materials reported to date is scarce, as magnetic structures that break inversion symmetry and induce an improper ferroelectric polarization typically arise through subtle competition between different magnetic interactions. The (NH4)2[FeCl5(H2O)] compound is a rare case wher...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep14475

    authors: Alberto Rodríguez-Velamazán J,Fabelo Ó,Millán Á,Campo J,Johnson RD,Chapon L

    更新日期:2015-09-29 00:00:00

  • MicroRNA-449a enhances radiosensitivity by downregulation of c-Myc in prostate cancer cells.

    abstract::MicroRNAs (miRNAs) have been reported to be involved in DNA damage response induced by ionizing radiation (IR). c-Myc is reduced when cells treated with IR or other DNA damaging agents. It is unknown whether miRNAs participate in c-Myc downregulation in response to IR. In the present study, we found that miR-449a enha...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep27346

    authors: Mao A,Zhao Q,Zhou X,Sun C,Si J,Zhou R,Gan L,Zhang H

    更新日期:2016-06-02 00:00:00

  • Ultrahigh field MR imaging of a subconjunctival anti-glaucoma drug delivery system in a rabbit model.

    abstract::Local drug delivery systems (DDS) have become a favourable approach for the treatment of numerous diseases. Biomedical imaging techniques such as ultrahigh field magnetic resonance imaging (UHF-MRI) offer unique insight into DDS biodegradation in vivo. We describe the establishment of a 7 Tesla MRI routine for longitu...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-15954-w

    authors: Kopp F,Eickner T,Polei S,Falke K,Witt M,Grabow N,Stachs O,Guthoff RF,Lindner T

    更新日期:2017-11-17 00:00:00

  • A microcosm approach highlights the response of soil mineral weathering bacterial communities to an increase of K and Mg availability.

    abstract::The access and recycling of the base cations are essential processes for the long-lasting functioning of forest ecosystems. While the role of soil bacterial communities has been demonstrated in mineral weathering and tree nutrition, our understanding of the link between the availability of base cations and the functio...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-50730-y

    authors: Nicolitch O,Feucherolles M,Churin JL,Fauchery L,Turpault MP,Uroz S

    更新日期:2019-10-07 00:00:00

  • Mutation Spectrum in the CACNA1A Gene in 49 Patients with Episodic Ataxia.

    abstract::Episodic ataxia is an autosomal dominant ion channel disorder characterized by episodes of imbalance and incoordination. The disease is genetically heterogeneous and is classified as episodic ataxia type 2 (EA2) when it is caused by a mutation in the CACNA1A gene, encoding the α1A subunit of the P/Q-type voltage-gated...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-02554-x

    authors: Sintas C,Carreño O,Fernàndez-Castillo N,Corominas R,Vila-Pueyo M,Toma C,Cuenca-León E,Barroeta I,Roig C,Volpini V,Macaya A,Cormand B

    更新日期:2017-05-31 00:00:00

  • Purification of an unpolarized spin ensemble into entangled singlet pairs.

    abstract::Dynamical polarization of nuclear spin ensembles is of central importance for magnetic resonance studies, precision sensing and for applications in quantum information theory. Here we propose a scheme to generate long-lived singlet pairs in an unpolarized nuclear spin ensemble which is dipolar coupled to the electron ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-00603-z

    authors: Greiner JN,Dasari DBR,Wrachtrup J

    更新日期:2017-04-03 00:00:00

  • Classification of Articulator Movements and Movement Direction from Sensorimotor Cortex Activity.

    abstract::For people suffering from severe paralysis, communication can be difficult or nearly impossible. Technology systems called brain-computer interfaces (BCIs) are being developed to assist these people with communication by using their brain activity to control a computer without any muscle activity. To benefit the devel...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-50834-5

    authors: Salari E,Freudenburg ZV,Branco MP,Aarnoutse EJ,Vansteensel MJ,Ramsey NF

    更新日期:2019-10-02 00:00:00

  • Advantages and limitations of microtiter biofilm assays in the model of antibiofilm activity of Klebsiella phage KP34 and its depolymerase.

    abstract::One of the potential antibiofilm strategies is to use lytic phages and phage-derived polysaccharide depolymerases. The idea is to uncover bacteria embedded in the biofilm matrix making them accessible and vulnerable to antibacterials and the immune system. Here we present the antibiofilm efficiency of lytic phage KP34...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-77198-5

    authors: Latka A,Drulis-Kawa Z

    更新日期:2020-11-23 00:00:00

  • Photoinhibition of Phaeocystis globosa resulting from oxidative stress induced by a marine algicidal bacterium Bacillus sp. LP-10.

    abstract::Harmful algal blooms caused by Phaeocystis globosa have resulted in staggering losses to coastal countries because of their world-wide distribution. Bacteria have been studied for years to control the blooms of harmful alga, however, the action mechanism of them against harmful algal cells is still not well defined. H...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep17002

    authors: Guan C,Guo X,Li Y,Zhang H,Lei X,Cai G,Guo J,Yu Z,Zheng T

    更新日期:2015-11-25 00:00:00

  • Heparin Forms Polymers with Cell-free DNA Which Elongate Under Shear in Flowing Blood.

    abstract::Heparin is a widely used anticoagulant which inhibits factor Xa and thrombin through potentiation of antithrombin. We recently identified that the nucleic acid stain SYTOX reacts with platelet polyphosphate due to molecular similarities, some of which are shared by heparin. We attempted to study heparin in flowing blo...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-54818-3

    authors: de Vries JC,Barendrecht AD,Clark CC,Urbanus RT,Boross P,de Maat S,Maas C

    更新日期:2019-12-04 00:00:00

  • Thermopriming reprograms metabolic homeostasis to confer heat tolerance.

    abstract::Heat stress threatens agriculture worldwide. Plants acquire heat stress tolerance through priming, which establishes stress memory during mild or severe transient heat stress. Such induced thermotolerance restructures metabolic networks and helps maintain metabolic homeostasis under heat stress. Here, we used an elect...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-36484-z

    authors: Serrano N,Ling Y,Bahieldin A,Mahfouz MM

    更新日期:2019-01-17 00:00:00

  • Size-tunable copper nanocluster aggregates and their application in hydrogen sulfide sensing on paper-based devices.

    abstract::Polystyrene sulfonate (PSS), a strong polyelectrolyte, was used to prepare red photoluminescent PSS-penicillamine (PA) copper (Cu) nanoclusters (NC) aggregates, which displayed high selectivity and sensitivity to the detection of hydrogen sulfide (H2S). The size of the PSS-PA-Cu NC aggregates could be readily controll...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep24882

    authors: Chen PC,Li YC,Ma JY,Huang JY,Chen CF,Chang HT

    更新日期:2016-04-26 00:00:00

  • Limiting asymmetric hearing improves benefits of bilateral hearing in children using cochlear implants.

    abstract::Neurodevelopmental changes occur with asymmetric hearing loss, limiting binaural/spatial hearing and putting children at risk for social and educational challenges. These deficits may be mitigated by providing bilateral hearing in children through auditory prostheses. Effects on speech perception and spatial hearing w...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-31546-8

    authors: Polonenko MJ,Papsin BC,Gordon KA

    更新日期:2018-09-04 00:00:00

  • An activator of mTOR inhibits oxLDL-induced autophagy and apoptosis in vascular endothelial cells and restricts atherosclerosis in apolipoprotein E⁻/⁻ mice.

    abstract::Oxidized low-density lipoprotein (oxLDL) inhibits mammalian target of rapamycin (mTOR) and induces autophagy and apoptosis in vascular endothelial cells (VECs) that play very critical roles for the cardiovascular homostasis. We recently defined 3-benzyl-5-((2-nitrophenoxy) methyl)-dihydrofuran-2(3H)-one (3BDO) as a ne...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep05519

    authors: Peng N,Meng N,Wang S,Zhao F,Zhao J,Su L,Zhang S,Zhang Y,Zhao B,Miao J

    更新日期:2014-07-01 00:00:00

  • Effects of veridical expectations on syntax processing in music: Event-related potential evidence.

    abstract::Numerous past studies have investigated neurophysiological correlates of music-syntactic processing. However, only little is known about how prior knowledge about an upcoming syntactically irregular event modulates brain correlates of music-syntactic processing. Two versions of a short chord sequence were presented re...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep19064

    authors: Guo S,Koelsch S

    更新日期:2016-01-19 00:00:00

  • A Micro-Thermal Sensor for Focal Therapy Applications.

    abstract::There is an urgent need for sensors deployed during focal therapies to inform treatment planning and in vivo monitoring in thin tissues. Specifically, the measurement of thermal properties, cooling surface contact, tissue thickness, blood flow and phase change with mm to sub mm accuracy are needed. As a proof of princ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep21395

    authors: Natesan H,Hodges W,Choi J,Lubner S,Dames C,Bischof J

    更新日期:2016-02-26 00:00:00

  • Grading criteria for chronic ocular graft-versus-host disease: comparing the NIH eye score, Japanese dry eye score, and DEWS 2007 score.

    abstract::Ocular graft-versus-host disease (GVHD) is a common complication after hematopoietic stem cell transplantation (HSCT). Here we compared the diagnostic rates of ocular GVHD, including its severity, prognosis and the agreement, obtained using three grading scales: the National Institutes of Health (NIH) eye score, Japan...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep06680

    authors: Tatematsu Y,Ogawa Y,Abe T,Kamoi M,Uchino M,Saijo-Ban Y,Yaguchi S,Mukai S,Mori T,Okamoto S,Tsubota K

    更新日期:2014-10-22 00:00:00

  • Oral intake of curcumin markedly activated CYP 3A4: in vivo and ex-vivo studies.

    abstract::Curcumin, a specific secondary metabolite of Curcuma species, has potentials for a variety of beneficial health effects. It is nowadays used as a dietary supplement. Everolimus (EVL) is an immunosuppressant indicated for allograft rejection and cancer therapy, but with narrow therapeutic window. EVL is a substrate of ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep06587

    authors: Hsieh YW,Huang CY,Yang SY,Peng YH,Yu CP,Chao PD,Hou YC

    更新日期:2014-10-10 00:00:00

  • High Temperature Deformation Mechanism in Hierarchical and Single Precipitate Strengthened Ferritic Alloys by In Situ Neutron Diffraction Studies.

    abstract::The ferritic Fe-Cr-Ni-Al-Ti alloys strengthened by hierarchical-Ni2TiAl/NiAl or single-Ni2TiAl precipitates have been developed and received great attentions due to their superior creep resistance, as compared to conventional ferritic steels. Although the significant improvement of the creep resistance is achieved in ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep45965

    authors: Song G,Sun Z,Li L,Clausen B,Zhang SY,Gao Y,Liaw PK

    更新日期:2017-04-07 00:00:00

  • Conformation-dependent binding of a Tetrastatin peptide to αvβ3 integrin decreases melanoma progression through FAK/PI3K/Akt pathway inhibition.

    abstract::Tetrastatin, a 230 amino acid sequence from collagen IV, was previously demonstrated to inhibit melanoma progression. In the present paper, we identified the minimal active sequence (QKISRCQVCVKYS: QS-13) that reproduced the anti-tumor effects of whole Tetrastatin in vivo and in vitro on melanoma cell proliferation, m...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-28003-x

    authors: Lambert E,Fuselier E,Ramont L,Brassart B,Dukic S,Oudart JB,Dupont-Deshorgue A,Sellier C,Machado C,Dauchez M,Monboisse JC,Maquart FX,Baud S,Brassart-Pasco S

    更新日期:2018-06-29 00:00:00

  • Deterioration of the Hanson Logboat: chemical and imaging assessment with removal of polyethylene glycol conserving agent.

    abstract::The state of preservation of wood in two samples from the Hanson Logboat, currently on display in Derby Museum and Art Gallery, was analysed using elemental analysis (EA), pyrolysis-gas chromatography/flame ionisation detection (Py-GC/FID), pyrolysis-gas chromatography/mass spectrometry (Py-GC/MS) and scanning electro...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-14057-w

    authors: Pinder AP,Panter I,Abbott GD,Keely BJ

    更新日期:2017-10-20 00:00:00

  • Network diffusion-based analysis of high-throughput data for the detection of differentially enriched modules.

    abstract::A relation exists between network proximity of molecular entities in interaction networks, functional similarity and association with diseases. The identification of network regions associated with biological functions and pathologies is a major goal in systems biology. We describe a network diffusion-based pipeline f...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep34841

    authors: Bersanelli M,Mosca E,Remondini D,Castellani G,Milanesi L

    更新日期:2016-10-12 00:00:00

  • Pre-RC Protein MCM7 depletion promotes mitotic exit by Inhibiting CDK1 activity.

    abstract::MCM7, a subunit of mini-chromosome maintenance proteins (MCM) complex, plays an important role in initiating DNA replication during the G1 phase and extending DNA strands during the S phase. Here, we demonstrated that MCM7 is not only sustained but maintains association with chromatin during M phase. Remarkably, MCM7 ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-03148-3

    authors: Zheng D,Ye S,Wang X,Zhang Y,Yan D,Cai X,Gao W,Shan H,Gao Y,Chen J,Hu Z,Li H,Li J

    更新日期:2017-06-06 00:00:00

  • Differential Variance Analysis: a direct method to quantify and visualize dynamic heterogeneities.

    abstract::Many amorphous materials show spatially heterogenous dynamics, as different regions of the same system relax at different rates. Such a signature, known as Dynamic Heterogeneity, has been crucial to understand the nature of the jamming transition in simple model systems and is currently considered very promising to ch...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep43496

    authors: Pastore R,Pesce G,Caggioni M

    更新日期:2017-03-14 00:00:00