当前位置： SCI文献检索 > INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES期刊下所有文献 > Hydrogen-Deuterium exchange kinetics in β-lactoglobulin (-)-epicatechin complexes studied by FTIR spectroscopy.

Hydrogen-Deuterium exchange kinetics in β-lactoglobulin (-)-epicatechin complexes studied by FTIR spectroscopy.

Abstract：

:Hydrogen-Deuterium exchange kinetics of β-lactoglobulin and β-lactoglobulin (-)-epicatechin solutions has been investigated through the analysis of the amide I absorption band at 1650cm-1 in the FTIR spectrum. H-D substitution in NH amides and residues of the protein results in a slight red-shift and in intensity changes of the amide I components: either these effects have been inspected in the framework of the Principal Components Analysis methods. The present analysis allowed to unveil three H-D kinetics at the timescale of the oligomeric fluctuations of the protein. A fast mechanism (lifetime from 5 to 10min) can be ascribed to the dynamics of protein oligomers and aggregates at the scale of the quaternary structure variations, and it is not observed in the complexes β-lactoglobulin (-)-epicatechin. The other slowest kinetics, whose lifetimes are in the range 1-10h, are here associated to dynamics of high-molecular weight complexes that hamper the proton exchange. The role of (-)-epicatechin as an enhancer of the formation of stable high-molecular weight aggregates from β-lactoglobulin is also discussed by comparison of the lifetimes at different protein concentrations.

journal_name

Int J Biol Macromol

journal_title

International journal of biological macromolecules

authors

Caporaletti F,Carbonaro M,Maselli P,Nucara A

doi

10.1016/j.ijbiomac.2017.06.028

subject

Has Abstract

pub_date

2017-11-01 00:00:00

pages

521-526

issue

Pt A

eissn

0141-8130

issn

1879-0003

pii

S0141-8130(17)30356-2

journal_volume

104

pub_type

杂志文章

相关文献

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES文献大全
  • Effect of hydroxypropyl-β-cyclodextrin on the bounding of salazosulfapyridine to human serum albumin.

    abstract：:The supramolecular interaction between salazosulfapyridine (SASP) and hydroxypropyl-β-cyclodextrin (HP-β-CD), as well as the influence of HP-β-CD on SASP's binding to human serum albumin (HSA), were investigated. Phase-solubility studies indicate that the HP-β-CD/SASP inclusion complex was formed at a 1:1 host-guest s...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2016.07.033

    authors： Tang P,Tang B,Wang Q,Xu K,Xiong X,Li H

    更新日期：2016-11-01 00:00:00

  • Overcoming stemness and chemoresistance in colorectal cancer through miR-195-5p-modulated inhibition of notch signaling.

    abstract：:MiR-195-5p has been shown to have a regulatory role in a variety of cancers. Its influence on colorectal cancer (CRC), however, has never been evaluated. In the present study, we found that miR-195-5p expression was significantly decreased as compared to paired, tumor-adjacent normal colorectal tissues. We demonstrate...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.05.151

    authors： Jin Y,Wang M,Hu H,Huang Q,Chen Y,Wang G

    更新日期：2018-10-01 00:00:00

  • Osteochondral and bone tissue engineering scaffold prepared from Gallus var domesticus derived demineralized bone powder combined with gellan gum for medical application.

    abstract：:Osteochondral (OC) lesions can occur in the knee and ankle. Such lesions induce a fracture in the cartilage protecting the bone joints. Cartilage tissue shows limited self-regeneration ability, hence the tissue is avascular and lack of vascular innervation, while the bone is a unique organ with the capacity to self-re...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.01.191

    authors： Kim D,Cho HH,Thangavelu M,Song C,Kim HS,Choi MJ,Song JE,Khang G

    更新日期：2020-04-15 00:00:00

  • The effect of aluminum oxide on red blood cell integrity and hemoglobin structure at nanoscale.

    abstract：:Herein, we explored the interaction of Al2O3 NPs with RBCs and Hb to determine the effect of Al2O3 NPs on hemolytic activity and Hb denaturation. The percentage of hemolysis of extracts and direct contact assays triggered by Al2O3 NPs was calculated by determining supernatant Hb concentration at 540 nm. Far-UV CD and ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.07.154

    authors： Kahbasi S,Samadbin M,Attar F,Heshmati M,Danaei D,Rasti B,Salihi A,Nanakali NMQ,Aziz FM,Akhtari K,Hasan A,Falahati M

    更新日期：2019-10-01 00:00:00

  • Genome-wide analysis of purple acid phosphatase (PAP) family proteins in Jatropha curcas L.

    abstract：:Purple acid phosphatase (PAP) family genes play a crucial role in the phosphorus (P) foraging and recycling. There are 25 putative Jatropha curcas PAP genes (JcrPAP) were identified and classified into three groups based on their molecular weights. Subcellular localization prediction indicated that most of the JcrPAPs...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.11.027

    authors： Venkidasamy B,Selvaraj D,Ramalingam S

    更新日期：2019-02-15 00:00:00

  • Alkaline proteases from a newly isolated Micromonospora chaiyaphumensis S103: Characterization and application as a detergent additive and for chitin extraction from shrimp shell waste.

    abstract：:The present study was undertaken to characterize the extracellular thermostable serine alkaline proteases from newly actinomycete strain Micromonospora chaiyaphumensis S103 and to describe their evaluation in commercial detergents and shrimp waste deproteinization. This proteolytic crude extract was active and stable ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2016.10.036

    authors： Mhamdi S,Ktari N,Hajji S,Nasri M,Sellami Kamoun A

    更新日期：2017-01-01 00:00:00

  • Biochemical and functional characterization of an atypical plant l-arginase from Cilantro (Coriandrum sativam L.).

    abstract：:Arginase is one of the key enzymes responsible for maintaining the essential levels of nitrogen among plants, but biochemical and functional characterization of arginase among plants is limited. While screening for stable plant arginase, we found cilantro possessing an abundant and stable arginase. We purified arginas...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.06.096

    authors： Siddappa S,Basrur V,Ravishankar Rai V,Marathe GK

    更新日期：2018-10-15 00:00:00

  • The effect of deep eutectic solvents on catalytic function and structure of bovine liver catalase.

    abstract：:Aqueous solutions of reline and glyceline, the most common deep eutectic solvents, were used as a medium for Catalase reaction. By some spectroscopic methods such as UV-vis, fluorescence and circular dichroism (CD) function and structure of Catalase were investigated in aqueous solutions of reline and glyceline. These...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2016.11.043

    authors： Harifi-Mood AR,Ghobadi R,Divsalar A

    更新日期：2017-02-01 00:00:00

  • Multiple fingerprint profiles and chemometrics analysis of polysaccharides from Sarcandra glabra.

    abstract：:Multiple techniques including high performance size-exclusion chromatography (HPSEC), Fourier-transform infrared spectroscopy (FT-IR) and pre-column derivatization high-performance liquid chromatography (PCD-HPLC) were applied to the fingerprint analysis of the polysaccharides from Sarcandra glabra (SGPs) in different...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.11.103

    authors： Li H,Gong X,Wang Z,Pan C,Zhao Y,Gao X,Liu W

    更新日期：2019-02-15 00:00:00

  • Effect of sulfation and partial hydrolysis of polysaccharides from Polygonatum sibiricum on immune-enhancement.

    abstract：:The aqueous polysaccharide from Polygonatum sibiricum was extracted and fractionated using anion-exchange chromatography to obtain F1 fraction. The F1 was chemically sulfated and partially acid-hydrolyzed for the production of its over-sulfated (OS1,2,3) and hydrolyzed (HP1,2,3) derivatives, in which the sulfate conte...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.10.119

    authors： Yelithao K,Surayot U,Park W,Lee S,Lee DH,You S

    更新日期：2019-02-01 00:00:00

  • Observation of in vitro cellulose synthesis by bacterial cellulose synthase with time-resolved small angle X-ray scattering.

    abstract：:Cellulose synthase is the enzyme that produces cellulose in the living organisms like plant, and has two functions: polymerizing glucose residues (polymerization) and assembling these polymerized molecules into a crystalline microfibril with a "cellulose I" crystallographic structure (crystallization). Many studies, h...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.02.167

    authors： Tajima H,Penttilä PA,Imai T,Yamamoto K,Yuguchi Y

    更新日期：2019-06-01 00:00:00

  • Sustainable supercapacitors of nitrogen-doping porous carbon based on cellulose nanocrystals and urea.

    abstract：:The development of porous carbon materials from sustainable natural sources is an attractive topic in the field of energy storage materials. This study proposed the production of nitrogen-doped porous carbon (NPC) materials from the renewable cellulose nanocrystal (CNC) as carbon source and water-soluble urea as nitro...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.09.011

    authors： Wang S,Dong L,Li Z,Lin N,Xu H,Gao S

    更新日期：2020-12-01 00:00:00

  • Chitosan-alginate immobilized lipase based catalytic constructs: Development, characterization and potential applications.

    abstract：:In this study, lipase (LIP) was isolated from Aspergillus crevinus, statistically optimized and purified via ammonium sulfate fractionation (ASF), and Sephadex G-100 gel permeation chromatography. LIP was 2.26-folds purified with a specific activity of 223.60 U/mg. The molecular mass was estimated to be 60 kDa on sodi...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.07.192

    authors： Rashid R,Anwar Z,Zafar M,Rashid T,Butt I

    更新日期：2018-11-01 00:00:00

  • Antivirus and immune enhancement activities of sulfated polysaccharide from Angelica sinensis.

    abstract：:This study is to synthesize sulfated Angelica polysaccharides (APSs) and investigate the activity of one of the sulfated derivatives APS-1 on murine leukemia virus in vivo. Six sulfated derivatives with degree of sulfation ranging from 0.68 to 1.91 were obtained. And the virus replication was inhibited by APS-1 at the...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2011.11.027

    authors： Yang T,Jia M,Zhou S,Pan F,Mei Q

    更新日期：2012-04-01 00:00:00

  • Transcriptomic analysis of differentially expressed genes and related pathways in Harmonia axyridis after sulfoxaflor exposure.

    abstract：:Harmonia axyridis (Pallas) is a major biological control agent that is indirectly threatened by sulfoxaflor insecticide applications targeting pests in agro-ecosystems. However, the genome-wide response of H. axyridis to sulfoxaflor remains largely unknown. Here, we used high-throughput RNA-seq technology to character...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.07.032

    authors： Nawaz M,Hafeez M,Mabubu JI,Dawar FU,Li X,Khan MM,Hua H,Cai W

    更新日期：2018-11-01 00:00:00

  • Isolation and processing of silk proteins for biomedical applications.

    abstract：:Silk proteins of silkworms are chiefly composed of core fibroin protein and glycoprotein sericin that glues fibroin. Unique mechanical properties, cyto-compatibility and controllable biodegradability facilitate the use of fibroin in biomedical applications. Sericin serves as additive in cosmetic and food industries, a...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2014.06.022

    authors： Kundu B,Kurland NE,Yadavalli VK,Kundu SC

    更新日期：2014-09-01 00:00:00

  • Liquisolid system of paclitaxel using modified polysaccharides: In vitro cytotoxicity, apoptosis study, cell cycle analysis, in vitro mitochondrial membrane potential assessment, and pharmacokinetics.

    abstract：:The research was aimed to develop a liquisolid formulation of paclitaxel using novel, highly porous liquisolid carriers (modified polysaccharides) to enhance bioavailability of orally administered paclitaxel. Modified polysaccharides namely co-grinded treated guar gum (C-TGG), co-grinded treated tamarind kernel powder...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.06.188

    authors： Sharma V,Pathak K

    更新日期：2019-09-15 00:00:00

  • Factors influencing the morphology and adsorption performance of cellulose nanocrystal/iron oxide nanorod composites for the removal of arsenic during water treatment.

    abstract：:The removal of arsenic from reservoirs is a matter of great concern in many parts of the world. Since adsorption is one of the most effective methods for treating arsenic-containing media, iron oxide nanorods were prepared using cellulose nanocrystals (CNs) as a template to remove this harmful metal from aqueous solut...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.11.182

    authors： Dong F,Xu X,Shaghaleh H,Guo J,Guo L,Qian Y,Liu H,Wang S

    更新日期：2020-08-01 00:00:00

  • Oral delivery of nanoparticles containing anticancer SN38 and hSET1 antisense for dual therapy of colon cancer.

    abstract：:An oral delivery system intended for treatment of colon cancer in HT29 cancerous cells was investigated by encapsulating hSET1 antisense and SN38 anticancer in nanoparticles based on cysteine trimethyl chitosan (cysTMC) and carboxymethyl dextran (CMD). Studies have shown hSET1 as the main type of histone methyltransfe...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2015.03.066

    authors： Dinarvand M,Kiani M,Mirzazadeh F,Esmaeili A,Mirzaie Z,Soleimani M,Dinarvand R,Atyabi F

    更新日期：2015-01-01 00:00:00

  • Injectable thermosensitive hybrid hydrogel containing graphene oxide and chitosan as dental pulp stem cells scaffold for bone tissue engineering.

    abstract：:Here, we fabricated thermosensitive injectable hydrogel containing poly (N-isopropylacrylamide) (PNIPAAm)-based copolymer/graphene oxide (GO) composite with different feed ratio to chitosan (CS) as a natural polymer through physical and chemical crosslinking for the proliferation and differentiation of the human denta...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.06.138

    authors： Amiryaghoubi N,Noroozi Pesyan N,Fathi M,Omidi Y

    更新日期：2020-11-01 00:00:00

  • Antitumor and antimetastatic activities of rhamnogalacturonan-II-type polysaccharide isolated from mature leaves of green tea via activation of macrophages and natural killer cells.

    abstract：:To investigate the antitumor and antimetastatic polysaccharide from the mature leaves of green tea, GTE-II was purified using size exclusion chromatography. GTE-II consisted of 15 different sugars including rarely observed sugars such as 2-O-methyl-fucose, 2-O-methyl-xylose, apiose, aceric acid, 3-deoxy-d-manno-2-octu...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2017.02.043

    authors： Park HR,Hwang D,Suh HJ,Yu KW,Kim TY,Shin KS

    更新日期：2017-06-01 00:00:00

  • Synthesis, antimicrobial activity, and sustainable release of novel α-aminophosphonate derivatives loaded carrageenan cryogel.

    abstract：:Novel α-aminophosphonates 4 were synthesized via one pot three-component reaction of 4-aminoantipyrine, aldehydes, triphenylphosphite and Lewis acid catalyst. The chemical structures of all the synthesized compounds were elucidated by IR, NMR and MS spectral analysis. The antimicrobial activity of 4 was tested in vitr...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.06.251

    authors： Elsherbiny DA,Abdelgawad AM,El-Naggar ME,El-Sherbiny RA,El-Rafie MH,El-Sayed IE

    更新日期：2020-11-15 00:00:00

  • Inhibition of methemoglobin formation in aqueous solutions under aerobic conditions by the addition of amino acids.

    abstract：:Hemoglobin (Hb) as an important iron-containing oxygen-transport protein is easily oxidized to the ferric met-form, methemoglobin (metHb), and loses the capacity of binding oxygen during storage. In this study, the experimental data indicate that the presence of Tyr and Glu significantly suppress the metHb formation i...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2013.12.010

    authors： Wei Y,Li C,Zhang L,Su Z,Xu X

    更新日期：2014-03-01 00:00:00

  • Investigation of inulins from the roots of Morinda officinalis for potential therapeutic application as anti-osteoporosis agent.

    abstract：SCOPE:In China, the root of M. officinalis has been widely used over thousands of years against a wide range of bone disease such as lumbago, limb-ache, sciatica and rheumatic arthralgia, and has tremendous medicinal value. But the bioactive constituents responsible for the osteoprotective effects in M. officinalis rem...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.08.082

    authors： Jiang K,Huang D,Zhang D,Wang X,Cao H,Zhang Q,Yan C

    更新日期：2018-12-01 00:00:00

  • The mechanism underlying OBP heterodimer formation and the recognition of odors in Holotrichia oblita Faldermann.

    abstract：:Insect odorant-binding proteins (OBPs) play key roles in transport odors to receptors and contribute to insect survival. The cooperative interaction of HoblOBP1 and HoblOBP2 in Holotrichia oblita Faldermann (Coleoptera: Melolonthidae) could increase their binding capacity for ligands. In present study, molecular docki...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.10.182

    authors： Wei HS,Duan HX,Li KB,Zhang S,Wei ZJ,Yin J

    更新日期：2020-06-01 00:00:00

  • Silencing of IL-6 and STAT3 by siRNA loaded hyaluronate-N,N,N-trimethyl chitosan nanoparticles potently reduces cancer cell progression.

    abstract：:The immunosuppressive nature of the tumor microenvironment is a critical problem that should be considered before the design of immunotherapies. Interleukin (IL)-6 and its related downstream molecules such as signal transducer and activator of transcription (STAT)3 play an important role in the cancer progression, whi...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.01.273

    authors： Masjedi A,Ahmadi A,Atyabi F,Farhadi S,Irandoust M,Khazaei-Poul Y,Ghasemi Chaleshtari M,Edalati Fathabad M,Baghaei M,Haghnavaz N,Baradaran B,Hojjat-Farsangi M,Ghalamfarsa G,Sabz G,Hasanzadeh S,Jadidi-Niaragh F

    更新日期：2020-04-15 00:00:00

  • Quantitative proteomics to reveal the composition of Southern India spectacled cobra (Naja naja) venom and its immunological cross-reactivity towards commercial antivenom.

    abstract：:Indian cobra (Naja naja) envenomation is frequently reported across Indian subcontinent. Geographical differences in the venom composition of a particular species of snake often leads to inconsistencies in the antivenom neutralization. Consequently, determining the venom proteome from every locale is necessary for the...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.05.106

    authors： Chanda A,Mukherjee AK

    更新日期：2020-10-01 00:00:00

  • Hydrothermal encapsulation of lanthanum oxide derived Aegle marmelos admixed chitosan bead system for nitrate and phosphate retention.

    abstract：:The potential of hydrothermal technology was utilized for the preparation of promising adsorbents in order to overcome the troubles of methemoglobinemia (blue baby syndrome) and eutrophication which is caused by excess nitrate (NO3-) and phosphate (PO43-) ions in water. Hence, in the present investigation, the chitosa...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.02.106

    authors： Kumar IA,Jeyaprabha C,Meenakshi S,Viswanathan N

    更新日期：2019-06-01 00:00:00

  • Characterization of Se-enriched Pleurotus ostreatus polysaccharides and their antioxidant effects in vitro.

    abstract：:Selenium-enriched polysaccharides have become a major research focus owing to their high anti-oxidant activities. Selenium-enriched Pleurotus ostreatus polysaccharide (Se-POP) and the native polysaccharide (POP) were obtained by hot water extraction from fresh fruiting bodies of P. ostreatus with or without selenium. ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2017.12.152

    authors： Ma L,Zhao Y,Yu J,Ji H,Liu A

    更新日期：2018-05-01 00:00:00

  • Partial characterization of a levan type exopolysaccharide (EPS) produced by Leuconostoc mesenteroides showing immunostimulatory and antioxidant activities.

    abstract：:Leuconostoc mesenteroides S81 was isolated from traditional sourdough as an exopolysaccharide (EPS) producer strain. The monosaccharide composition of the EPS from strain S81 was characterized by HPLC analysis and only fructose was found in the repeating unit structure. The NMR spectroscopy analysis revealed that EPS ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.06.078

    authors： Taylan O,Yilmaz MT,Dertli E

    更新日期：2019-09-01 00:00:00