Abstract:
:Interactions between chronic lymphocytic leukemia (CLL) B cells and the bone marrow (BM) microenvironment play a major function in the physiopathology of CLL. Extracellular vesicles (EVs), which are composed of exosomes and microparticles, play an important role in cell communication. However, little is known about their role in CLL / microenvironment interactions. In the present study, EVs purified by ultracentrifugation from BM mesenchymal stromal cell (BM-MSC) cultures were added to CLL B cells. After their integration into CLL B cells, we observed a decrease of leukemic cell spontaneous apoptosis and an increase in their chemoresistance to several drugs, including fludarabine, ibrutinib, idelalisib and venetoclax after 24 hours. Spontaneous (P=0.0078) and stromal cell-derived factor 1α -induced migration capacities of CLL B cells were also enhanced (P=0.0020). A microarray study highlighted 805 differentially expressed genes between leukemic cells cultured with or without EVs. Of these, genes involved in the B-cell receptor pathway such as CCL3/4, EGR1/2/3, and MYC were increased. Interestingly, this signature presents important overlaps with other microenvironment stimuli such as B-cell receptor stimulation, CLL/nurse-like cells co-culture or those provided by a lymph node microenvironment. Finally, we showed that EVs from MSCs of leukemic patients also rescue leukemic cells from spontaneous or drug-induced apoptosis. However, they induce a higher migration and also a stronger gene modification compared to EVs of healthy MSCs. In conclusion, we show that EVs play a crucial role in CLL B cells/BM microenvironment communication.
journal_name
Haematologicajournal_title
Haematologicaauthors
Crompot E,Van Damme M,Pieters K,Vermeersch M,Perez-Morga D,Mineur P,Maerevoet M,Meuleman N,Bron D,Lagneaux L,Stamatopoulos Bdoi
10.3324/haematol.2016.163337subject
Has Abstractpub_date
2017-09-01 00:00:00pages
1594-1604issue
9eissn
0390-6078issn
1592-8721pii
haematol.2016.163337journal_volume
102pub_type
杂志文章相关文献
HAEMATOLOGICA文献大全abstract:BACKGROUND:Allogeneic hematopoietic cell transplantation is a potentially curative treatment for patients with acute lymphoblastic leukemia. However, the majority of older adults with acute lymphoblastic leukemia are not candidates for myeloablative conditioning regimens. A non-myeloablative preparative regimen is a re...
journal_title:Haematologica
pub_type: 临床试验,杂志文章,多中心研究
doi:10.3324/haematol.2011.040261
更新日期:2011-08-01 00:00:00
abstract:BACKGROUND:An effective second-line treatment for intermediate and high grade non-Hodgkin's lymphoma is greatly needed since 30% of patients do not achieved complete remission (CR) and another 20% to 30% of the CRs will eventually relapse. METHODS:A four-drug combination with Mitoxantrone, Etoposide, Cisplatin and Dex...
journal_title:Haematologica
pub_type: 临床试验,杂志文章
doi:
更新日期:1991-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:A D-dimer assay can be helpful to rule out thromboembolism provided it is sensitive, reliable, fast and easy to perform. Tests based on the ELISA methodology have a high diagnostic sensitivity, and are therefore adequate for excluding deep venous thrombosis (DVT). The drawbacks are their long a...
journal_title:Haematologica
pub_type: 杂志文章
doi:
更新日期:2000-05-01 00:00:00
abstract::Atrial natriuretic peptide (ANP) levels have been tested in patients with chronic relative polycythemia (RP), polycythemia vera, and in healthy subjects in order to find a possible underlying pathophysiological mechanism for relative polycythemia. No difference in statistical significance has been found between the me...
journal_title:Haematologica
pub_type: 杂志文章
doi:
更新日期:1989-07-01 00:00:00
abstract:BACKGROUND:Dyskeratosis congenita is a cancer-prone bone marrow failure syndrome caused by aberrations in telomere biology. DESIGN AND METHODS:We studied 65 patients with dyskeratosis congenita and 127 unaffected relatives. Telomere length was measured by automated multicolor flow fluorescence in situ hybridization in...
journal_title:Haematologica
pub_type: 临床试验,杂志文章
doi:10.3324/haematol.2011.055269
更新日期:2012-03-01 00:00:00
abstract:BACKGROUND:The genetic characterization of chronic lymphocytic leukemia cells correlates with the behavior, progression and response to treatment of the disease. DESIGN AND METHODS:Our aim was to investigate the role of ATM gene alterations, their biological consequences and their value in predicting disease progressi...
journal_title:Haematologica
pub_type: 杂志文章
doi:10.3324/haematol.2011.049270
更新日期:2012-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:The efficacy and safety of added dexamethasone were assessed in patients with relapsed and/or refractory multiple myeloma who had a suboptimal response to bortezomib alone. DESIGN AND METHODS:In two previously reported, open-label, multicenter phase 2 studies, bortezomib 1.0 or 1.3 mg/m2 was ...
journal_title:Haematologica
pub_type: 杂志文章,多中心研究,随机对照试验
doi:
更新日期:2006-07-01 00:00:00
abstract::The lupus anticoagulant (LAC) and anticardiolipin antibody (ACA) syndromes require particular therapeutic approaches: thrombotic accidents are an indication for oral anticoagulant therapy (OAT), whereas severe thrombocytopenia may require the special treatments used for immunologic thrombocytopenic purpura (ITP). We d...
journal_title:Haematologica
pub_type: 杂志文章
doi:
更新日期:1995-05-01 00:00:00
abstract::With the deaths of Janet Rowley and John Goldman in December 2013, the world lost two pioneers in the field of chronic myeloid leukemia. In 1973, Janet Rowley, unraveled the cytogenetic anatomy of the Philadelphia chromosome, which subsequently led to the identification of the BCR-ABL1 fusion gene and its principal pa...
journal_title:Haematologica
pub_type: 传,历史文章,杂志文章,评审
doi:10.3324/haematol.2015.139337
更新日期:2016-05-01 00:00:00
abstract::Hodgkin's lymphoma (HL) simulating acute cholestatic hepatitis is a very unusual clinical picture. Massive liver infiltration with conspicuous acute cholestatic symptoms, in fact, suggests more aggressive systemic diseases such as non Hodgkin's lymphomas or acute leukemias. Nevertheless, we observed two cases of mixed...
journal_title:Haematologica
pub_type: 杂志文章
doi:
更新日期:1989-05-01 00:00:00
abstract::Effects of angiotensin (Ang)-(1-7), an AngII metabolite, on bone marrow-derived hematopoietic cells were studied. We identified Ang-(1-7) to stimulate proliferation of human CD34(+) and mononuclear cells in vitro. Under in vivo conditions, we monitored proliferation and differentiation of human cord blood mononuclear ...
journal_title:Haematologica
pub_type: 杂志文章
doi:10.3324/haematol.2008.000034
更新日期:2009-06-01 00:00:00
abstract::Patients with chronic lymphocytic leukemia and unmutated immunoglobulin heavy-chain variable region gene (IGHV) have inferior survival from time of treatment in clinical studies. We assessed real-world outcomes based on mutational status and treatment regimen in a nationwide population-based cohort, comprising all 4,1...
journal_title:Haematologica
pub_type: 杂志文章
doi:10.3324/haematol.2019.220194
更新日期:2020-06-01 00:00:00
abstract::The objective of this study was to create a bioclinical model, based on clinical and molecular predictors of event-free and overall survival for relapsed/refractory diffuse large B-cell lymphoma patients treated on the Canadian Cancer Trials Group (CCTG) LY12 prospective study. In 91 cases, sufficient histologic mater...
journal_title:Haematologica
pub_type: 临床试验,杂志文章
doi:10.3324/haematol.2017.179309
更新日期:2018-02-01 00:00:00
abstract::Alemtuzumab serum levels and clinical response after subcutaneous administration (10 mg 3 times/week for six weeks) have been explored in 29 chronic lymphocytic leukemia patients receiving the monoclonal antibody as consolidation. Serum concentrations after each administration gradually increased during the first week...
journal_title:Haematologica
pub_type: 临床试验,杂志文章
doi:10.3324/haematol.2010.033159
更新日期:2011-06-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:With the development and refinement of guidance modalities for percutaneous biopsies, many investigators have reported studies supporting the role of guided core needle biopsy in the diagnosis of mediastinal lymphoma. The aims of this report are to evaluate the efficacy of findings at core need...
journal_title:Haematologica
pub_type: 杂志文章
doi:
更新日期:1999-07-01 00:00:00
abstract::Lenalidomide is a very active drug in myelodysplastic syndrome with del (5q). We report such a patient treated with this drug who developed unusual complex cytogenetic abnormalities, which were elucidated by multi-FISH and FISH analysis as jumping translocations involving the long arm of chromosome 5, that resulted in...
journal_title:Haematologica
pub_type: 信件
doi:10.3324/haematol.11917
更新日期:2008-02-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Identification and characterization of hematopoietic stem cells in peripheral blood (PB) and cord blood (CB) have suggested feasible alternatives to conventional allogeneic bone marrow (BM) transplantation. The growing interest in this use of allogeneic stem cells has prompted the Working Group...
journal_title:Haematologica
pub_type: 杂志文章,评审
doi:
更新日期:1997-03-01 00:00:00
abstract:BACKGROUND:In trials designed to evaluate new therapies for hematologic malignancies, end points such as leukemia-free survival are often used as surrogates for overall survival in acute leukemia. We aimed to assess whether leukemia-free survival is an acceptable statistical surrogate for overall survival when applied ...
journal_title:Haematologica
pub_type: 杂志文章,随机对照试验
doi:10.3324/haematol.2010.039131
更新日期:2011-08-01 00:00:00
abstract:BACKGROUND:There is little published information on the everyday clinical management of myelodysplastic syndromes in real world practice. DESIGN AND METHODS:We conducted a cross-sectional study of all patients with myelodysplastic syndromes attending 74 French centers in a 1-week period for inpatient admission, day-ho...
journal_title:Haematologica
pub_type: 杂志文章,多中心研究
doi:10.3324/haematol.2009.014357
更新日期:2010-06-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:How to reach the correct diagnosis of a lymph node enlargement is still a problem which strongly challenges the knowledge and experience of the clinician. Organized and specifically oriented literature on the right sequential steps and the logical criteria that should guide this diagnostic appr...
journal_title:Haematologica
pub_type: 杂志文章,评审
doi:
更新日期:1999-03-01 00:00:00
abstract::Blackfan-Diamond anemia (BDA) is a rare hypoproliferative anemia occurring in infancy or in early childhood. Patients who fail on usual doses of steroids did not achieve remission with other pharmacological agents. Claims that other molecules such as cyclosporin A (CSA) or antithymocyte globulin (ATG) are effective re...
journal_title:Haematologica
pub_type: 杂志文章
doi:
更新日期:1993-01-01 00:00:00
abstract::Multiple myeloma is preceded by an asymptomatic phase, comprising monoclonal gammopathy of uncertain significance and smoldering myeloma. Compared to the former, smoldering myeloma has a higher and non-uniform rate of progression to clinical myeloma, reflecting a subset of patients with higher risk. We evaluated the g...
journal_title:Haematologica
pub_type: 临床试验,杂志文章,多中心研究
doi:10.3324/haematol.2015.124651
更新日期:2015-09-01 00:00:00
abstract::Distinct expression patterns of pro- and anti-apoptotic proteins may contribute to different prognoses and therapy outcomes in adult versus childhood acute myeloid leukemia (AML). Therefore, we investigated whether expression levels of apoptosis-related proteins CD95, Bcl-2, Bax, Bcl-xL, procaspase-3, XIAP, cIAP-1, an...
journal_title:Haematologica
pub_type: 信件
doi:
更新日期:2004-03-01 00:00:00
abstract::Older chronic lymphocytic leukemia patients have poor outcomes with standard treatments and are underrepresented in clinical trials. We retrospectively reviewed outcomes of refractory chronic lymphocytic leukemia patients in two age categories (≥70 and <70 years) treated with single-agent flavopiridol, a drug active i...
journal_title:Haematologica
pub_type: 杂志文章
doi:10.3324/haematol.2011.047324
更新日期:2012-03-01 00:00:00
abstract:BACKGROUND:Increased levels of microparticles exposing tissue factor circulate in the blood of patients with coronary heart disease, possibly disseminating their pro-thrombotic and pro-inflammatory potential. Because diets rich in n-3 (polyunsaturated) fatty acids have been associated with reduced incidence of coronary...
journal_title:Haematologica
pub_type: 杂志文章
doi:10.3324/haematol.11845
更新日期:2008-06-01 00:00:00
abstract:BACKGROUND:31D8 monoclonal antibody (mAb) has been shown to bind heterogeneously to human neutrophils, identifying subsets of cells which differ in their functional response to chemotactic stimuli. In this study we used 31D8 mAb to determine whether differences in neutrophil subpopulations might explain the long-lastin...
journal_title:Haematologica
pub_type: 杂志文章
doi:
更新日期:1995-03-01 00:00:00
abstract::Leukemia stem cells (LSC) are more resistant to standard chemotherapy and their persistence during remission can cause relapse, which is still one of the major clinical challenges in the treatment of acute myeloid leukemia (AML). A better understanding of the mutational patterns and the prognostic impact of molecular ...
journal_title:Haematologica
pub_type: 杂志文章
doi:10.3324/haematol.2019.225003
更新日期:2020-03-01 00:00:00
abstract:BACKGROUND:Hemoglobin sickle cell disease is one of the most frequent hemoglobinopathies. Surprisingly, few studies have been dedicated to this disease, currently considered to be a mild variant of homozygous sickle cell disease. The aim of this study was to update our knowledge about hemoglobin sickle cell disease. D...
journal_title:Haematologica
pub_type: 杂志文章
doi:10.3324/haematol.2011.055202
更新日期:2012-08-01 00:00:00
abstract::To our knowledge, there is little information about functional thrombin activatable fibrinolysis inhibitor (TAFI) levels and the risk of acute coronary artery disease (CAD). We investigated the risk of acute CAD related to plasma levels of functional TAFI. We found that functional TAFI levels in plasma (above 126%), i...
journal_title:Haematologica
pub_type: 信件
doi:
更新日期:2004-07-01 00:00:00
abstract::We studied the impact of comorbidities on survival and evaluated the prognostic utility of comorbidity scores in MDS patients, who received best supportive care and were assessable according to the Charlson Comorbidity Index (CCI) and the Hematopoietic Stem Cell Transplantation Comorbidity Index (HCTCI): 171 patients ...
journal_title:Haematologica
pub_type: 杂志文章
doi:10.3324/haematol.2008.002063
更新日期:2009-05-01 00:00:00