Abstract:
:Recent advances in fluorescent protein technology provide a wide variety of biological imaging applications; however current tools for bio-imaging in the Gram-positive bacterium Staphylococcus aureus has necessitated further developments for fluorescence intensity and for a multicolor palette of fluorescent proteins. To enhance the expression of multicolor fluorescent proteins in clinical S. aureus strains, we developed new fluorescent protein expression vectors, containing the blaZ/sodp promoter consisting of the β-lactamase gene (blaZ) promoter and the ribosome binding site (RBS) of superoxide dismutase gene (sod). We found S. aureus-adapted GFP (GFPsa) driven by the blaZ/sodp promoter was highly expressed in the S. aureus laboratory strain RN4220, but not in the clinical strains, MW2 and N315, harboring the endogenous blaI gene, a repressor of the blaZ gene promoter. We therefore constructed a constitutively induced blaZ/sodp promoter (blaZ/sodp(Con)) by introducing substitution mutations into the BlaI binding motif, and this modification allowed enhanced expression of the multicolor GFP variants (GFPsa, EGFP, mEmerald, Citrine, Cerulean, and BFP) as well as codon-optimized reef coral fluorescent proteins (mCherry and AmCyan) in the S. aureus clinical strains. These new fluorescent probes provide new tools to enhance expression of multicolor fluorescent proteins and facilitate clear visualization of clinical S. aureus strains.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Kato F,Nakamura M,Sugai Mdoi
10.1038/s41598-017-02930-7subject
Has Abstractpub_date
2017-06-06 00:00:00pages
2865issue
1issn
2045-2322pii
10.1038/s41598-017-02930-7journal_volume
7pub_type
杂志文章abstract::It is well known that the Gobi Desert is the dominant source area of the Badain Jaran Desert (BJD) and the Chinese Loess Plateau (CLP). However, due to the absence of quantitative analyses, there are nearly no exact assessments of its actual contribution. Combinations of field investigations, wind tunnel experiments, ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-37635-y
更新日期:2019-01-30 00:00:00
abstract::Bacterial biofilms are particularly problematic since they become resistant to most available antibiotics. Hence, novel potential antagonists to inhibit biofilm formation are urgent. Here the influences of two natural products, ursolic acid and resveratrol, on biofilm of the clinical methicillin-resistant Staphylococc...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep05467
更新日期:2014-06-27 00:00:00
abstract::During pregnancy a variety of immunological changes occur to accommodate the fetus. It is unknown whether these changes continue to affect humoral immunity postpartum or how quickly they resolve. IgG levels were measured to P. falciparum and P. vivax antigens in 201 postpartum and 201 controls over 12 weeks. Linear mi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep32159
更新日期:2016-08-25 00:00:00
abstract::Humans are good at selectively listening to specific target conversations, even in the presence of multiple concurrent speakers. In our research, we study how auditory-visual cues modulate this selective listening. We do so by using immersive Virtual Reality technologies with spatialized audio. Exposing 32 participant...
journal_title:Scientific reports
pub_type: 临床试验,杂志文章
doi:10.1038/s41598-017-04201-x
更新日期:2017-06-19 00:00:00
abstract::Bovine tuberculosis (BTB) testing in cattle requires a significant investment of time, equipment, and labor. Novel, rapid, cheaper and accurate methods are needed. The Alere Determine TB lipoarabinomannan antigen (LAM-test) is a World Health Organization-endorsed point-of-care urine test designed to detect active TB d...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-62314-2
更新日期:2020-03-25 00:00:00
abstract::The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has resulted in thousands of deaths in the world. Information about prediction model of prognosis of SARS-CoV-2 infection is scarce. We used machine learning for processing laboratory findings of 110 patients with SARS-CoV-2 pneumonia (including 51 non-s...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-71114-7
更新日期:2020-08-20 00:00:00
abstract::Head and neck squamous cell carcinoma (HNSCC) development is a multistage process includes the normal, dysplasia and squamous cell carcinoma (SCC) stages. Recently, increasing evidence has suggested that the tumor microenvironment (TME) is an integral part of malignant transformation. Exploring certain key node genes ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep20208
更新日期:2016-02-01 00:00:00
abstract::Recently, the organic functionalization of polyoxometalates (POMs) has drawn increasing interest, and an easy and effective route to achieve organic derivatives is of great importance. Herein, the first reported synthesis of a tosyl ester derivative of the polyoxometalate (Bu4N)2[V6O13{(OCH2)3CCH2SO3C7H4}2]·2.5CH3CN (...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-12633-8
更新日期:2017-10-02 00:00:00
abstract::Accumulating evidences have suggested the potential association between Int7G24A (rs334354) polymorphism and cancer risk. However, results from epidemiological studies are controversial. We thus conducted this meta-analysis to clarify the association. Relevant studies were identified on electronic databases according ...
journal_title:Scientific reports
pub_type: 杂志文章,meta分析
doi:10.1038/srep11350
更新日期:2015-06-15 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Scientific reports
pub_type: 已发布勘误
doi:10.1038/s41598-020-61338-y
更新日期:2020-03-06 00:00:00
abstract::Multiplexed error-robust fluorescence in situ hybridization (MERFISH) allows simultaneous imaging of numerous RNA species in their native cellular environment and hence spatially resolved single-cell transcriptomic measurements. However, the relatively modest brightness of signals from single RNA molecules can become ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-43943-8
更新日期:2019-05-22 00:00:00
abstract::Chromatin remodeling has been newly established as an important cancer genome characterization and recent exome and whole-genome sequencing studies of hepatocellular carcinoma (HCC) showed that recurrent inactivating mutations in SWI/SNF subunits involved in the molecular basis of hepatocarcinogenesis. To test the hyp...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep04147
更新日期:2014-02-21 00:00:00
abstract::Microbial rhodopsin is a photoreceptor protein found in various bacteria and archaea, and it is considered to be a light-utilization device unique to heterotrophs. Recent studies have shown that several cyanobacterial genomes also include genes that encode rhodopsins, indicating that these auxiliary light-utilizing pr...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-73606-y
更新日期:2020-10-07 00:00:00
abstract::BPA is an oestrogenic endocrine disrupting chemical compound. Exposure to BPA in as early as pregnancy leads to lifelong effects. Since endocrine and immune systems interact in a bidirectional manner, endocrine disruption may cause permanent alterations of the immune system, affecting a future anti-tumoral response. N...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-10135-1
更新日期:2017-09-05 00:00:00
abstract::Recently, extracorporeal shock wave therapy (ESWT) has been shown to be a novel therapy for carpal tunnel syndrome (CTS). However, previous studies did not examine the diverse effects of different-session ESWT for different-grades CTS. Thus, we conducted a randomized, single-blind, placebo-controlled study. Sixty-nine...
journal_title:Scientific reports
pub_type: 杂志文章,随机对照试验
doi:10.1038/srep38344
更新日期:2016-12-02 00:00:00
abstract::Cell fusion can occur between mesenchymal stem cells (MSCs) transplanted to improve cardiac function and cells of the recipient. The therapeutic benefit or detriment of resultant cell hybrids is unknown. Here we augment fusion of transplanted MSCs with recipient cardiac cell types via viral fusogens to determine how c...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep20283
更新日期:2016-02-05 00:00:00
abstract::Refractive index sensors based on surface plasmon resonance (SPR) promise to deliver high sensitivities. However, these sensitivities depend on the derivative of the monitored SPR parameters near resonance, so this dependency leads to a relatively narrow detection range for refractive index changes. Herein, we introdu...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-41353-4
更新日期:2019-03-26 00:00:00
abstract::The strong adhesion of sub-micron sized particles to surfaces is a nuisance, both for removing contaminating colloids from surfaces and for conscious manipulation of particles to create and test novel micro/nano-scale assemblies. The obvious idea of using detergents to ease these processes suffers from a lack of contr...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep36443
更新日期:2016-11-03 00:00:00
abstract::The authors present the preparation procedure and a computational model of a three-layered fibrous scaffold for prolonged drug release. The scaffold, produced by emulsion/sequential electrospinning, consists of a poly(D,L-lactic-co-glycolic acid) (PLGA) fiber layer sandwiched between two poly(ε-caprolactone) (PCL) lay...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-68117-9
更新日期:2020-07-07 00:00:00
abstract::This paper reports on a tunable transmission frequency characteristics-based metamaterial absorber of an X band sensing application with a fractional bandwidth. Tunable resonator metamaterial absorbers fabricated with dielectric surface have been the subject of growing attention of late. Absorbers possess electromagne...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-69792-4
更新日期:2020-08-04 00:00:00
abstract::Randomizing the layer thickness of superlattices (SL) can lead to localization of coherent phonons and thereby reduces the lattice thermal conductivity κ l . In this work, we propose strategies that can suppress incoherent phonon transport in the above random multilayer (RML) structure to further reduce κ l . Molecula...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-08359-2
更新日期:2017-08-15 00:00:00
abstract::This study aimed to investigate whether single nucleotide polymorphisms (SNPs) of five NLR family genes (NOD1, NOD2, NLRP1, NLRP3 and CIITA) are associated with Behcet's disease (BD) in a Chinese Han population. The study was carried out in 950 BD patients and 1440 controls for 19 SNPs in the selected NLR genes. In th...
journal_title:Scientific reports
pub_type: 临床试验,杂志文章,多中心研究
doi:10.1038/srep20098
更新日期:2016-02-01 00:00:00
abstract::The primary hurdle for diagnosis of some diseases is the long incubation required to culture and confirm the presence of bacteria. The concept of using microbial VOCs as "signature markers" could provide a faster and noninvasive diagnosis. Finding biomarkers is challenging due to the specificity required in complex ma...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-47907-w
更新日期:2019-08-20 00:00:00
abstract::This study was to assess the safety and efficacy of artificial ascitetes in assisting CT-guided cryoablation of hepatic tumors adjacent to the gastrointestinal (GI) tract. A total of 84 patients with peripheral hepatic tumors adjacent to the GI tract, who were treated cryoablation, were included in this retrospective ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-17023-8
更新日期:2017-11-30 00:00:00
abstract::Nitrogen vacancy (NV) centers in diamond have been used as ultrasensitive magnetometers to perform nuclear magnetic resonance (NMR) spectroscopy of statistically polarized samples at 1-100 nm length scales. However, the spectral linewidth is typically limited to the kHz level, both by the NV sensor coherence time and ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-43404-2
更新日期:2019-05-06 00:00:00
abstract::Disseminated diseases of the central nervous system such as amyotrophic lateral sclerosis (ALS) require that therapeutic agents are delivered and distributed broadly. Intrathecal route is attractive in that respect, but to date there was no methodology available allowing for optimization of this technique to assure sa...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-34723-x
更新日期:2018-11-07 00:00:00
abstract::Stable electroluminescence from micro-pixelated light-emitting diode (μLED) occurs when electrons and holes are continuously injected from external electrodes. Different from the general recognition, in this work, μLED works in an operation mode, namely, non-electrical contact and non-carrier injection mode, and can b...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-65092-z
更新日期:2020-05-15 00:00:00
abstract::Graphene-based materials have been studied in many applications, owing to the excellent electrical, mechanical, and thermal properties of graphene. In the current study, an environmentally friendly approach to the preparation of a reduced graphene oxide-gold nanoparticle (rGO-AuNP) nanocomposite was developed by using...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-15210-1
更新日期:2017-11-03 00:00:00
abstract::Despite strengthened antimicrobial therapy, biofilm infections of Acinetobacter baumannii are associated with poor prognosis and limited therapeutic options. Assessing antibiotics on planktonic bacteria can result in failure against biofilm infections. Currently, antibiotics to treat biofilm infections are administere...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-42353-0
更新日期:2019-04-19 00:00:00
abstract::Peptides derived from the N-terminal heptad repeat (NHR) of HIV-1 gp41 can be potent inhibitors against viral entry when presented in a nonaggregating trimeric coiled-coil conformation via the introduction of exogenous trimerization motifs and intermolecular disulfide bonds. We recently discovered that crosslinking is...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep32161
更新日期:2016-08-26 00:00:00