Corneal Potential Maps Measured With Multi-Electrode Electroretinography in Rat Eyes With Experimental Lesions.

Abstract:

Purpose:Conventional full-field flash electroretinography (ERG) yields a single response waveform that can be useful in the early detection and diagnosis of many diseases affecting the retina. It is an objective measurement that probes the entire retina. However, localized areas of dysfunction have relatively small influence on ERG amplitudes compared to normal ranges. Here we evaluate the use of corneal potential maps obtained in response to full-field flash stimuli for sensitivity to local areas of retinal damage. Methods:A contact lens electrode array was used to record 25 ERG waveforms simultaneously following saturating full-field flash stimuli (multi-electrode electroretinography, meERG) in rats. Waveforms were evaluated for a-wave and b-wave amplitudes; these values were normalized and further evaluated for spatial differences across the corneal surface. Cluster analysis and a support vector machine approach were used to classify meERG responses from healthy eyes and eyes with central (photocoagulation) or peripheral (cryocoagulation) experimental lesions. Results:A normative normalized corneal potential map was obtained from healthy eyes (n = 26). Corneal potential maps from eyes with experimental lesions (n = 13) could be classified with sensitivity and specificity of approximately 80% based solely on the normalized spatial distribution of corneal potentials, that is, with no knowledge of absolute amplitudes. Conclusions:Corneal potential maps obtained in response to full-field flash stimuli are altered in eyes with scotomas in the central and far-peripheral retina. The meERG approach yields useful spatial information following a single brief flash, analogous to body-surface potential maps used to evaluate heart and brain.

authors

Derafshi Z,Kunzer BE,Mugler EM,Rokhmanova N,Park DW,Tajalli H,Shetty K,Ma Z,Williams JC,Hetling JR

doi

10.1167/iovs.16-20726

subject

Has Abstract

pub_date

2017-06-01 00:00:00

pages

2863-2873

issue

7

eissn

0146-0404

issn

1552-5783

pii

2630799

journal_volume

58

pub_type

杂志文章
  • Decrease of anterior ciliary arterial pressure with increased ocular pressure.

    abstract::The relationship of the ocular pressure to the anterior ciliary arterial pressure was studied by measuring the pressure of the anterior ciliary artery using a modified pressure chamber. Of the 40 subjects, 9 were normal, 16 were ocular hypertensive patients, and 15 were primary, open-angle, glaucoma patients. A signif...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Sakimoto G,Schwartz B

    更新日期:1984-08-01 00:00:00

  • Local and Systemic Inflammatory Biomarkers of Diabetic Retinopathy: An Integrative Approach.

    abstract:Purpose:To review the usefulness of local and systemic inflammatory biomarkers of diabetic retinopathy (DR) to implement a more personalized treatment. Methods:An integrated research (from ophthalmologist and diabetologist point of view) of most significant literature on serum, vitreous, and aqueous humor (AH) biochem...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章,评审

    doi:10.1167/iovs.17-21769

    authors: Vujosevic S,Simó R

    更新日期:2017-05-01 00:00:00

  • A double-blind placebo-controlled study to evaluate valacyclovir alone and with aspirin for asymptomatic HSV-1 DNA shedding in human tears and saliva.

    abstract:PURPOSE:To test the effect of valacyclovir alone and with aspirin on the asymptomatic shedding of HSV-1 DNA in tears and saliva of healthy individuals. METHOD. The subjects (n = 45) were randomized into three groups without regard to age, sex, or race. Group 1 (n = 14) received the placebo, group 2 (n = 15) received a ...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章,随机对照试验

    doi:10.1167/iovs.09-3729

    authors: Kumar M,Hill JM,Clement C,Varnell ED,Thompson HW,Kaufman HE

    更新日期:2009-12-01 00:00:00

  • The Relationship Between Helicobacter pylori Infection and Open-Angle Glaucoma: A Meta-Analysis.

    abstract:PURPOSE:Previous studies assessed the associations between Helicobacter pylori infection and open-angle glaucoma (OAG) and produced inconsistent results. Therefore, we performed a meta-analysis to evaluate the effects of H. pylori infection on OAG risk. METHODS:Relevant studies that reported the associations between H...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章,meta分析,评审

    doi:10.1167/iovs.15-17059

    authors: Zeng J,Liu H,Liu X,Ding C

    更新日期:2015-08-01 00:00:00

  • Repeated injections of a ciliary neurotrophic factor analogue leading to long-term photoreceptor survival in hereditary retinal degeneration.

    abstract:PURPOSE:To determine whether ciliary neurotrophic factor (CNTF) or brain-derived neurotrophic factor (BDNF) treatment leads to long-term photoreceptor survival in hereditary retinal degeneration. METHODS:An autosomal dominant feline model of rod-cone dystrophy was used throughout the study with two normal animals. In ...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Chong NH,Alexander RA,Waters L,Barnett KC,Bird AC,Luthert PJ

    更新日期:1999-05-01 00:00:00

  • A Novel Motion-on-Color Paradigm for Isolating Magnocellular Pathway Function in Preperimetric Glaucoma.

    abstract:PURPOSE:This study investigated a novel motion-on-color paradigm to functionally isolate the magnocellular pathway and evaluate its diagnostic value in preperimetric glaucoma patients. METHODS:Thirty patients with preperimetric primary open-angle glaucoma and 30 controls participated in this study. They were tested in...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.15-16394

    authors: Wen W,Zhang P,Liu T,Zhang T,Gao J,Sun X,He S

    更新日期:2015-07-01 00:00:00

  • Assessment of central visual function in Stargardt's disease/fundus flavimaculatus with ultrahigh-resolution optical coherence tomography.

    abstract:PURPOSE:To assess photoreceptor morphology in patients with Stargardt's disease and fundus flavimaculatus using ultrahigh-resolution optical coherence tomography (UHR-OCT) and correlate it with visual acuity (VA). METHODS:This was a prospective observational case series. Fourteen patients with Stargardt's disease (nin...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.04-0212

    authors: Ergun E,Hermann B,Wirtitsch M,Unterhuber A,Ko TH,Sattmann H,Scholda C,Fujimoto JG,Stur M,Drexler W

    更新日期:2005-01-01 00:00:00

  • Effects of lomerizine, a calcium channel antagonist, on retinal and optic nerve head circulation in rabbits and humans.

    abstract:PURPOSE:To investigate the effects of lomerizine, a Ca2+ antagonist, on the ocular tissue circulation in rabbits and on the circulation in the optic nerve head (ONH) and choroid in healthy volunteers. METHODS:Lomerizine (0.1 [n=10] or 0.3 [n=11] mg/kg) or vehicle solution (n=11) was injected intravenously in urethane-...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 临床试验,杂志文章

    doi:10.1167/iovs.02-1173

    authors: Tamaki Y,Araie M,Fukaya Y,Nagahara M,Imamura A,Honda M,Obata R,Tomita K

    更新日期:2003-11-01 00:00:00

  • Retinal basement membrane abnormalities and the retinopathy of Alport syndrome.

    abstract:PURPOSE:To determine the effects of X-linked and autosomal recessive Alport syndrome on retinal basement membranes and how these result in the characteristic perimacular dot-and-fleck retinopathy, lozenge, and macular hole. METHODS:The type IV collagen chains present in the normal retina were determined immunohistoche...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章,多中心研究

    doi:10.1167/iovs.08-3323

    authors: Savige J,Liu J,DeBuc DC,Handa JT,Hageman GS,Wang YY,Parkin JD,Vote B,Fassett R,Sarks S,Colville D

    更新日期:2010-03-01 00:00:00

  • Lens-specific expression of a major histocompatibility complex class I molecule disrupts normal lens development and induces cataracts in transgenic mice.

    abstract:PURPOSE:Lens epithelial tissue does not normally express major histocompatibility complex (MHC) class I molecules. In addition, the mechanism of self-tolerance to intraocular antigens is unknown. To study the effect of class I expression in the lens, transgenic mice were produced that express an allo-MHC class I molecu...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Martin WD,Egan RM,Stevens JL,Woodward JG

    更新日期:1995-05-01 00:00:00

  • Redistribution of Mn++-dependent pyrimidine 5'-nucleotidase (MDPNase) activity during shedding and phagocytosis.

    abstract::Ultrastructural cytochemistry was used to localize a previously undescribed retinal enzyme activity, ie, "manganese-dependent pyrimidine 5'-nucleotidase" (MDPNase) activity in retinas from rats raised in cyclic light and killed at various times in the lighting cycle. In retinas fixed during the last 3 hr of darkness, ...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Irons MJ

    更新日期:1987-01-01 00:00:00

  • Glycogen and DNA content of corneal epithelium: comparison of preparation methods.

    abstract::Three methods of corneal epithelial preparation for subsequent analysis of epithelial glycogen and DNA content were compared: scraping, lamellar stripping, and in situ freezing. All three methods gave good recovery of these substances. However, there was incomplete recovery of the sample using the in situ freezing met...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Friend J,Kiorpes T,Kinoshita S

    更新日期:1983-02-01 00:00:00

  • Decrease in rate of myopia progression with a contact lens designed to reduce relative peripheral hyperopia: one-year results.

    abstract:PURPOSE:To determine whether a novel optical treatment using contact lenses to reduce relative peripheral hyperopia can slow the rate of progress of myopia. METHODS:Chinese children, aged 7 to 14 years, with baseline myopia from sphere -0.75 to -3.50 D and cylinder ≤1.00 D, were fitted with novel contact lenses (n = 4...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章,随机对照试验

    doi:10.1167/iovs.11-7260

    authors: Sankaridurg P,Holden B,Smith E 3rd,Naduvilath T,Chen X,de la Jara PL,Martinez A,Kwan J,Ho A,Frick K,Ge J

    更新日期:2011-12-09 00:00:00

  • Retinomotor pigment migration in the teleost retinal pigment epithelium. II. Cyclic-3',5'-adenosine monophosphate induction of dark-adaptive movement in vitro.

    abstract::The retinal pigment epithelium (RPE) and photoreceptors of teleosts exhibit dramatic examples of cell motility (called retinomotor movements) in response to diurnal changes in lighting conditions. In darkness the pigment granules of the RPE migrate to the scleral base of the RPE cell and cone photoreceptors elongate. ...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Burnside B,Basinger S

    更新日期:1983-01-01 00:00:00

  • Effects of selenium on ion homeostasis and transparency in cultured lenses.

    abstract::Selenium toxicity was investigated in cultured rabbit lenses to provide further information about the role of Ca++ in Se cataract. At a dose of 0.1 mM for 20 hr, Se induces a 10% change in Na levels within 6 hr, a 30% increase after 20 hr, and a three-fold increase within 48 hr of subsequent culture after removal of S...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Hightower KR,McCready JP

    更新日期:1989-01-01 00:00:00

  • Single choroideremia gene in nonmammalian vertebrates explains early embryonic lethality of the zebrafish model of choroideremia.

    abstract:PURPOSE:Mutations of the CHM gene underlie the X-linked chorioretinal degeneration choroideremia (CHM). The affected gene product, Rab Escort Protein (REP)1, mediates the posttranslational prenyl modification of Rab GTPases. In patients with CHM, the related REP2 partially compensates for the loss of function of REP1. ...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.08-2755

    authors: Moosajee M,Tulloch M,Baron RA,Gregory-Evans CY,Pereira-Leal JB,Seabra MC

    更新日期:2009-06-01 00:00:00

  • Swelling-activated potassium channel in porcine pigmented ciliary epithelial cells.

    abstract:PURPOSE:Ion channels in the ciliary epithelium play critical roles in the formation of aqueous humor in the eye. The present study identified a novel, swelling-activated K(+) current in freshly dissociated porcine pigmented ciliary epithelial cells. METHODS:Ciliary epithelial cells were freshly dissociated from porcin...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.11-7173

    authors: Takahira M,Sakurai M,Sakurada N,Sugiyama K

    更新日期:2011-07-29 00:00:00

  • Neural network classification of corneal topography. Preliminary demonstration.

    abstract:PURPOSE:Videokeratography is a powerful tool for the diagnosis of corneal shape abnormalities. However, interpretation of the topographic map is sometimes difficult, especially when pathologies with similar topographic patterns are suspected. The neural networks model, an artificial intelligence approach, was applied f...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Maeda N,Klyce SD,Smolek MK

    更新日期:1995-06-01 00:00:00

  • Regulation of paracrine cytokine balance controlling collagenase synthesis by corneal cells.

    abstract:PURPOSE:Classic studies have demonstrated that corneal epithelial cell density in culture can alter the balance of stimulatory and inhibitory cytokines controlling the elaboration of collagenolytic activity by co-cultured stromal cells. The current study attempts to bring the understanding of this mechanism to a molecu...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Strissel KJ,Rinehart WB,Fini ME

    更新日期:1997-02-01 00:00:00

  • Role of Endothelium in Abnormal Cannabidiol-Induced Vasoactivity in Retinal Arterioles.

    abstract:PURPOSE:Cannabinoids have been reported to mediate changes in vascular resistance through endothelial receptor targets. We examined involvement of the endothelium in cannabinoid-mediated vasoactive responses in resistance arterioles of the retina. METHODS:Vascular responses to both intraluminal (IL) and extraluminal (...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.14-14879

    authors: Su EN,Kelly ME,Cringle SJ,Yu DY

    更新日期:2015-06-01 00:00:00

  • Therapeutic Effects of a Novel Phenylphthalimide Analog for Corneal Neovascularization and Retinal Vascular Leakage.

    abstract:Purpose:Neovascularization (NV) and retinal vascular leakage are major causes of impaired vision in ocular diseases. The purpose of this study was to identify novel phenylphthalimide analogs with therapeutic effects on NV and vascular leakage and to explore the mechanism of action. Methods:Antiangiogenic activities of...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.18-24015

    authors: Wang B,Li PK,Ma JX,Chen D

    更新日期:2018-07-02 00:00:00

  • Orbital Fibroblasts From Graves' Orbitopathy Patients Share Functional and Immunophenotypic Properties With Mesenchymal Stem/Stromal Cells.

    abstract:PURPOSE:In Graves' orbitopathy (GO), inflammation and expansion of the retrobulbar tissue are the result of a pathophysiologic process in which orbital fibroblasts (GO-Fs) are considered the central cell type. However, in a previous study we observed that GO-Fs expressed some of the consensus surface markers described ...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.15-16610

    authors: Brandau S,Bruderek K,Hestermann K,Görtz GE,Horstmann M,Mattheis S,Lang S,Eckstein A,Berchner-Pfannschmidt U

    更新日期:2015-10-01 00:00:00

  • Adaptation of saccadic and vestibulo-ocular systems after extraocular muscle tenectomy.

    abstract::Adaptation of saccadic magnitude and vestibulo-ocular reflex gain (VOR) was examined in six monkeys that had undergone a tenectomy of the medial and lateral recti of one eye. After the tenectomy, when the normal eye was patched, a conjugate increase in saccadic magnitude and VOR was observed in both eyes. Subsequent u...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Snow R,Hore J,Vilis T

    更新日期:1985-07-01 00:00:00

  • Incomplete cortical reorganization in macular degeneration.

    abstract:PURPOSE:Activity in regions of the visual cortex corresponding to central scotomas in subjects with macular degeneration (MD) is considered evidence for functional reorganization in the brain. Three unresolved issues related to cortical activity in subjects with MD were addressed: Is the cortical response to stimuli pr...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.09-4926

    authors: Liu T,Cheung SH,Schuchard RA,Glielmi CB,Hu X,He S,Legge GE

    更新日期:2010-12-01 00:00:00

  • Imaging Laser-Induced Choroidal Neovascularization in the Rodent Retina Using Optical Coherence Tomography Angiography.

    abstract:PURPOSE:The purpose of this study was to evaluate the performance of optical coherence tomography angiography (OCTA) in visualizing laser-induced choroidal neovascularization (CNV) in the rodent retina. METHODS:Choroidal neovascularization was induced via laser photocoagulation in 2 male Brown Norway rats and 2 male C...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.15-18946

    authors: Park JR,Choi W,Hong HK,Kim Y,Jun Park S,Hwang Y,Kim P,Joon Woo S,Hyung Park K,Oh WY

    更新日期:2016-07-01 00:00:00

  • Studies on bicarbonate transporters and carbonic anhydrase in porcine nonpigmented ciliary epithelium.

    abstract:PURPOSE:Bicarbonate transport plays a role in aqueous humor (AH) secretion. The authors examined bicarbonate transport mechanisms and carbonic anhydrase (CA) in porcine nonpigmented ciliary epithelium (NPE). METHODS:Cytoplasmic pH (pH(i)) was measured in cultured porcine NPE loaded with BCECF. Anion exchanger (AE), so...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.08-2487

    authors: Shahidullah M,To CH,Pelis RM,Delamere NA

    更新日期:2009-04-01 00:00:00

  • Murine model of ocular infection by a human biovar of Chlamydia trachomatis.

    abstract:PURPOSE:A human biovar of Chlamydia trachomatis was used to develop a murine model of ocular chlamydial infection. The inbred mouse model will allow detailed immunologic studies during ocular infection, and use of a human biovar for infection may aid in identification of appropriate vaccine strategies against chlamydia...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Whittum-Hudson JA,O'Brien TP,Prendergast RA

    更新日期:1995-09-01 00:00:00

  • Spantide I decreases type I cytokines, enhances IL-10, and reduces corneal perforation in susceptible mice after Pseudomonas aeruginosa infection.

    abstract:PURPOSE:To determine the effects of blocking substance P (SP) interactions with its major receptor (NK1-R) using the antagonist spantide I in susceptible mice infected with Pseudomonas aeruginosa. METHODS:Immunohistochemistry and enzyme immunosorbent assay (EIA) tested levels of SP in the cornea of B6 and BALB/c mice....

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.06-0882

    authors: Hazlett LD,McClellan SA,Barrett RP,Liu J,Zhang Y,Lighvani S

    更新日期:2007-02-01 00:00:00

  • Rod photoreceptors in infant rats with a history of oxygen exposure.

    abstract:PURPOSE:To study in an infant rat model of retinopathy of prematurity, the rod photoreceptors, which are known to have attenuated photoresponses. METHODS:Rhodopsin was extracted from whole retinas, the thickness of the rod outer segment (ROS) layer was measured, large phagosomes were counted, and the ROS ultrastructur...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Fulton AB,Reynaud X,Hansen RM,Lemere CA,Parker C,Williams TP

    更新日期:1999-01-01 00:00:00

  • Characterization of effector T cells in dry eye disease.

    abstract:PURPOSE:Dry eye disease (DED) is associated with ocular surface inflammation that is thought to be mediated primarily by CD4 T cells. The purpose of this study was to investigate whether this T cell-mediated immune response is generated in the lymphoid compartment and to characterize the functional phenotype of the T c...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.08-2417

    authors: El Annan J,Chauhan SK,Ecoiffier T,Zhang Q,Saban DR,Dana R

    更新日期:2009-08-01 00:00:00