Single-peptide DNA-dependent RNA polymerase homologous to multi-subunit RNA polymerase.

Abstract:

:Transcription in all living organisms is accomplished by multi-subunit RNA polymerases (msRNAPs). msRNAPs are highly conserved in evolution and invariably share a ∼400 kDa five-subunit catalytic core. Here we characterize a hypothetical ∼100 kDa single-chain protein, YonO, encoded by the SPβ prophage of Bacillus subtilis. YonO shares very distant homology with msRNAPs, but no homology with single-subunit polymerases. We show that despite homology to only a few amino acids of msRNAP, and the absence of most of the conserved domains, YonO is a highly processive DNA-dependent RNA polymerase. We demonstrate that YonO is a bona fide RNAP of the SPβ bacteriophage that specifically transcribes its late genes, and thus represents a novel type of bacteriophage RNAPs. YonO and related proteins present in various bacteria and bacteriophages have diverged from msRNAPs before the Last Universal Common Ancestor, and, thus, may resemble the single-subunit ancestor of all msRNAPs.

journal_name

Nat Commun

journal_title

Nature communications

authors

Forrest D,James K,Yuzenkova Y,Zenkin N

doi

10.1038/ncomms15774

subject

Has Abstract

pub_date

2017-06-06 00:00:00

pages

15774

issn

2041-1723

pii

ncomms15774

journal_volume

8

pub_type

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