Discovery of nitrate-CPK-NLP signalling in central nutrient-growth networks.


:Nutrient signalling integrates and coordinates gene expression, metabolism and growth. However, its primary molecular mechanisms remain incompletely understood in plants and animals. Here we report unique Ca2+ signalling triggered by nitrate with live imaging of an ultrasensitive biosensor in Arabidopsis leaves and roots. A nitrate-sensitized and targeted functional genomic screen identifies subgroup III Ca2+-sensor protein kinases (CPKs) as master regulators that orchestrate primary nitrate responses. A chemical switch with the engineered mutant CPK10(M141G) circumvents embryo lethality and enables conditional analyses of cpk10 cpk30 cpk32 triple mutants to define comprehensive nitrate-associated regulatory and developmental programs. Nitrate-coupled CPK signalling phosphorylates conserved NIN-LIKE PROTEIN (NLP) transcription factors to specify the reprogramming of gene sets for downstream transcription factors, transporters, nitrogen assimilation, carbon/nitrogen metabolism, redox, signalling, hormones and proliferation. Conditional cpk10 cpk30 cpk32 and nlp7 mutants similarly impair nitrate-stimulated system-wide shoot growth and root establishment. The nutrient-coupled Ca2+ signalling network integrates transcriptome and cellular metabolism with shoot-root coordination and developmental plasticity in shaping organ biomass and architecture.






Liu KH,Niu Y,Konishi M,Wu Y,Du H,Sun Chung H,Li L,Boudsocq M,McCormack M,Maekawa S,Ishida T,Zhang C,Shokat K,Yanagisawa S,Sheen J




Has Abstract


2017-05-18 00:00:00
















  • Changes in the tooth enamel of early Paleocene mammals allowing increased diet diversity.

    abstract::Hunter-Schreger bands (HSB) are seen in teeth that are composed of crossed sets of enamel prisms. They are present in the teeth of man and many other mammals, but absent in most insectivores and multituberculates. It has been suggested that the presence of HSB makes the tooth enamel less likely to split and is associa...


    pub_type: 杂志文章


    authors: von Koenigswald W,Rensberger JM,Pretzschner HU

    更新日期:1987-07-09 00:00:00

  • In vivo alteration of telomere sequences and senescence caused by mutated Tetrahymena telomerase RNAs.

    abstract::Mutating the CAACCCCAA sequence in the RNA component of telomerase causes the synthesis in vivo of new telomere sequences corresponding to the mutated RNA sequence, demonstrating that the telomerase contains the template for telomere synthesis. These mutations also lead to nuclear and cell division defects, and senesc...


    pub_type: 杂志文章


    authors: Yu GL,Bradley JD,Attardi LD,Blackburn EH

    更新日期:1990-03-08 00:00:00

  • A clue to the basic defect in cystic fibrosis from cloning the CF antigen gene.

    abstract::The metabolic basis of the autosomal recessive disease cystic fibrosis (CF) remains unidentified. Elevated levels of a serum protein in CF homozygotes and obligate heterozygotes have been described. As heterozygotes are clinically unaffected, any consistently observed abnormality in these individuals is a likely point...


    pub_type: 杂志文章


    authors: Dorin JR,Novak M,Hill RE,Brock DJ,Secher DS,van Heyningen V

    更新日期:1987-04-09 00:00:00

  • Structure of the Fc fragment of human IgE bound to its high-affinity receptor Fc epsilonRI alpha.

    abstract::The initiation of immunoglobulin-E (IgE)-mediated allergic responses requires the binding of IgE antibody to its high-affinity receptor, Fc epsilonRI. Crosslinking of Fc epsilonRI initiates an intracellular signal transduction cascade that triggers the release of mediators of the allergic response. The interaction of ...


    pub_type: 杂志文章


    authors: Garman SC,Wurzburg BA,Tarchevskaya SS,Kinet JP,Jardetzky TS

    更新日期:2000-07-20 00:00:00

  • The tungsten isotopic composition of the Earth's mantle before the terminal bombardment.

    abstract::Many precious, 'iron-loving' metals, such as gold, are surprisingly abundant in the accessible parts of the Earth, given the efficiency with which core formation should have removed them to the planet's deep interior. One explanation of their over-abundance is a 'late veneer'--a flux of meteorites added to the Earth a...


    pub_type: 杂志文章


    authors: Willbold M,Elliott T,Moorbath S

    更新日期:2011-09-07 00:00:00

  • Assembly of microtubule-associated protein tau into Alzheimer-like filaments induced by sulphated glycosaminoglycans.

    abstract::The paired helical filament (PHF) is the major component of the neurofibrillary deposits that form a defining neuropathological characteristic of Alzheimer's disease. PHFs are composed of microtubule-associated protein tau, in a hyperphosphorylated state. Hyperphosphorylation of tau results in its inability to bind to...


    pub_type: 杂志文章


    authors: Goedert M,Jakes R,Spillantini MG,Hasegawa M,Smith MJ,Crowther RA

    更新日期:1996-10-10 00:00:00

  • The open pore conformation of potassium channels.

    abstract::Living cells regulate the activity of their ion channels through a process known as gating. To open the pore, protein conformational changes must occur within a channel's membrane-spanning ion pathway. KcsA and MthK, closed and opened K(+) channels, respectively, reveal how such gating transitions occur. Pore-lining '...


    pub_type: 杂志文章


    authors: Jiang Y,Lee A,Chen J,Cadene M,Chait BT,MacKinnon R

    更新日期:2002-05-30 00:00:00

  • Robotics: self-reproducing machines.

    abstract::Self-reproduction is central to biological life for long-term sustainability and evolutionary adaptation. Although these traits would also be desirable in many engineered systems, the principles of self-reproduction have not been exploited in machine design. Here we create simple machines that act as autonomous modula...


    pub_type: 杂志文章


    authors: Zykov V,Mytilinaios E,Adams B,Lipson H

    更新日期:2005-05-12 00:00:00

  • Impaired T(H)17 cell differentiation in subjects with autosomal dominant hyper-IgE syndrome.

    abstract::The autosomal dominant hyper-IgE syndrome (HIES, 'Job's syndrome') is characterized by recurrent and often severe pulmonary infections, pneumatoceles, eczema, staphylococcal abscesses, mucocutaneous candidiasis, and abnormalities of bone and connective tissue. Mutations presumed to underlie HIES have recently been ide...


    pub_type: 杂志文章


    authors: Milner JD,Brenchley JM,Laurence A,Freeman AF,Hill BJ,Elias KM,Kanno Y,Spalding C,Elloumi HZ,Paulson ML,Davis J,Hsu A,Asher AI,O'Shea J,Holland SM,Paul WE,Douek DC

    更新日期:2008-04-10 00:00:00

  • Prodigious degassing of a billion years of accumulated radiogenic helium at Yellowstone.

    abstract::Helium is used as a critical tracer throughout the Earth sciences, where its relatively simple isotopic systematics is used to trace degassing from the mantle, to date groundwater and to time the rise of continents. The hydrothermal system at Yellowstone National Park is famous for its high helium-3/helium-4 isotope r...


    pub_type: 杂志文章


    authors: Lowenstern JB,Evans WC,Bergfeld D,Hunt AG

    更新日期:2014-02-20 00:00:00

  • Isolation of two novel candidate hormones using a chemical method for finding naturally occurring polypeptides.

    abstract::Naturally occurring peptides with biological actions have in most cases been detected by observing their biological activities in crude extracts and their isolation has been followed using bioassays. As a complement to the classical biological detection systems, we have proposed a chemical detection system based on fr...


    pub_type: 杂志文章


    authors: Tatemoto K,Mutt V

    更新日期:1980-06-05 00:00:00

  • A possible neuronal basis for representation of acoustic scenes in auditory cortex of the big brown bat.

    abstract::Behavioural studies and field observations demonstrate that echolocating bats simultaneously perceive range, direction and shape of multiple objects in the environment as acoustic images derived from echoes. Cortical echo delay-tuned neurons contribute to the perception of object range, because focal inactivation of t...


    pub_type: 杂志文章


    authors: Dear SP,Simmons JA,Fritz J

    更新日期:1993-08-12 00:00:00

  • Structural alteration of hair cells in the contralateral ear resulting from extracochlear electrical stimulation.

    abstract::Chronic electrical stimulation of the auditory nerve in patients with profound sensori-neural deafness is becoming increasingly routine. Therefore, it is important to understand more about the long-term consequences of this procedure. Hitherto, structural studies in animals after electrocochlear stimulation have conce...


    pub_type: 杂志文章


    authors: Dodson HC,Walliker JR,Frampton S,Douek EE,Fourcin AJ,Bannister LH

    更新日期:1986-03-06 00:00:00

  • Homologous expressed genes in the human sex chromosome pairing region.

    abstract::The human sex chromosomes share a pair of homologous genes which independently encode a cell-surface antigen defined by the monoclonal antibody 12E7 (refs 1, 2; see refs 3, 4 for review). The X-located homologue, MIC2X, escapes X-inactivation and the equivalent Y-located locus, MIC2Y, was one of the first genes shown ...


    pub_type: 杂志文章


    authors: Buckle V,Mondello C,Darling S,Craig IW,Goodfellow PN

    更新日期:1985-10-24 00:00:00

  • Cdk1 is sufficient to drive the mammalian cell cycle.

    abstract::Unicellular organisms such as yeasts require a single cyclin-dependent kinase, Cdk1, to drive cell division. In contrast, mammalian cells are thought to require the sequential activation of at least four different cyclin-dependent kinases, Cdk2, Cdk3, Cdk4 and Cdk6, to drive cells through interphase, as well as Cdk1 t...


    pub_type: 杂志文章


    authors: Santamaría D,Barrière C,Cerqueira A,Hunt S,Tardy C,Newton K,Cáceres JF,Dubus P,Malumbres M,Barbacid M

    更新日期:2007-08-16 00:00:00

  • Enkephalin-, VIP- and substance P-like immunoreactivity in the carotid body.

    abstract::The carotid body type I cell contains amines and has features, both morphological and cytochemical, which indicate that it may also produce a peptide. Many regulatory peptides are now known to be present in both central and peripheral tissues. In the periphery these neuropeptides occur in both classical endocrine (APU...


    pub_type: 杂志文章


    authors: Wharton J,Polak JM,Pearse AG,McGregor GP,Bryant MG,Bloom SR,Emson PC,Bisgard GE,Will JA

    更新日期:1980-03-20 00:00:00

  • Susceptibility to murine lymphocytic choriomeningitis maps to class I MHC genes--a model for MHC/disease associations.

    abstract::Susceptibility to some human diseases is linked, albeit weakly, to major transplantation antigens (HLA) encoded by the major histocompatibility gene complex (MHC). Here we have studied MHC/disease association in inbred strains of mice after intracerebral (i.c.) injection of lymphocytic choriomeningitis virus (LCMV). T...


    pub_type: 杂志文章


    authors: Zinkernagel RM,Pfau CJ,Hengartner H,Althage A

    更新日期:1985-08-29 00:00:00

  • The physical mechanisms of fast radio bursts.

    abstract::Fast radio bursts are mysterious millisecond-duration transients prevalent in the radio sky. Rapid accumulation of data in recent years has facilitated an understanding of the underlying physical mechanisms of these events. Knowledge gained from the neighbouring fields of gamma-ray bursts and radio pulsars has also of...


    pub_type: 杂志文章,评审


    authors: Zhang B

    更新日期:2020-11-01 00:00:00

  • Adjuvant-free IgG responses induced with antigen coupled to antibodies against class II MHC.

    abstract::The generation of strong serological responses to protein antigens in experimental animals usually requires the use of potent adjuvants, most of which cannot be used in human or veterinary vaccines because of deleterious side effects. Attempting to circumvent this problem, we have assessed an adjuvant-free antigen-del...


    pub_type: 杂志文章


    authors: Carayanniotis G,Barber BH

    更新日期:1987-05-07 00:00:00

  • Density dependence explains tree species abundance and diversity in tropical forests.

    abstract::The recurrent patterns in the commonness and rarity of species in ecological communities--the relative species abundance--have puzzled ecologists for more than half a century. Here we show that the framework of the current neutral theory in ecology can easily be generalized to incorporate symmetric density dependence....


    pub_type: 杂志文章


    authors: Volkov I,Banavar JR,He F,Hubbell SP,Maritan A

    更新日期:2005-12-01 00:00:00

  • Genome organization and transactivation of the human immunodeficiency virus type 2.

    abstract::Analysis of the nucleotide sequence of the human retrovirus associated with AIDS in West Africa, HIV-2, shows that it is evolutionarily distant from the previously characterized HIV-1. We suggest that these viruses existed long before the current AIDS epidemics. Their biological properties are conserved in spite of li...


    pub_type: 杂志文章


    authors: Guyader M,Emerman M,Sonigo P,Clavel F,Montagnier L,Alizon M

    更新日期:1987-04-16 00:00:00

  • Underdetection of cases of COVID-19 in France threatens epidemic control.

    abstract::As countries in Europe gradually relaxed lockdown restrictions after the first wave, test-trace-isolate strategies became critical to maintain the incidence of coronavirus disease 2019 (COVID-19) at low levels1,2. Reviewing their shortcomings can provide elements to consider in light of the second wave that is current...


    pub_type: 杂志文章


    authors: Pullano G,Di Domenico L,Sabbatini CE,Valdano E,Turbelin C,Debin M,Guerrisi C,Kengne-Kuetche C,Souty C,Hanslik T,Blanchon T,Boëlle PY,Figoni J,Vaux S,Campèse C,Bernard-Stoecklin S,Colizza V

    更新日期:2020-12-21 00:00:00

  • Hinf family: a novel repeated DNA family of the human genome.

    abstract::The isolation of a mutant adenovirus carrying an insertion of cellular DNA has led to the identification of a new family of human repetitive sequences, which are found tandemly arranged in the genome. The sequence of the viral insert resembles that of eukaryotic transposable elements. ...


    pub_type: 杂志文章


    authors: Shimizu Y,Yoshida K,Ren CS,Fujinaga K,Rajagopalan S,Chinnadurai G

    更新日期:1983-04-14 00:00:00

  • Role of Tet1 in erasure of genomic imprinting.

    abstract::Genomic imprinting is an allele-specific gene expression system that is important for mammalian development and function. The molecular basis of genomic imprinting is allele-specific DNA methylation. Although it is well known that the de novo DNA methyltransferases Dnmt3a and Dnmt3b are responsible for the establishme...


    pub_type: 杂志文章


    authors: Yamaguchi S,Shen L,Liu Y,Sendler D,Zhang Y

    更新日期:2013-12-19 00:00:00

  • Moving beyond microbiome-wide associations to causal microbe identification.

    abstract::Microbiome-wide association studies have established that numerous diseases are associated with changes in the microbiota. These studies typically generate a long list of commensals implicated as biomarkers of disease, with no clear relevance to disease pathogenesis. If the field is to move beyond correlations and beg...


    pub_type: 杂志文章


    authors: Surana NK,Kasper DL

    更新日期:2017-12-14 00:00:00

  • Quantum phase transition in a common metal.

    abstract::The classical theory of solids, based on the quantum mechanics of single electrons moving in periodic potentials, provides an excellent description of substances ranging from semiconducting silicon to superconducting aluminium. Over the last fifteen years, it has become increasingly clear that there are substances for...


    pub_type: 杂志文章


    authors: Yeh A,Soh YA,Brooke J,Aeppli G,Rosenbaum TF,Hayden SM

    更新日期:2002-10-03 00:00:00

  • Differential inhibition of neurone-neurone, neurone-astrocyte and astrocyte-astrocyte adhesion by L1, L2 and N-CAM antibodies.

    abstract::The cell adhesion molecules L1, N-CAM and Ng-CAM have been implicated in cell-cell interactions among developing neural cells. L1 and N-CAM are structurally and functionally distinct molecular entities and act synergistically in mediating Ca2+-independent adhesion between re-aggregating early postnatal cerebellar cell...


    pub_type: 杂志文章


    authors: Keilhauer G,Faissner A,Schachner M

    更新日期:1985-08-22 00:00:00

  • A gene expression atlas of the central nervous system based on bacterial artificial chromosomes.

    abstract::The mammalian central nervous system (CNS) contains a remarkable array of neural cells, each with a complex pattern of connections that together generate perceptions and higher brain functions. Here we describe a large-scale screen to create an atlas of CNS gene expression at the cellular level, and to provide a libra...


    pub_type: 杂志文章


    authors: Gong S,Zheng C,Doughty ML,Losos K,Didkovsky N,Schambra UB,Nowak NJ,Joyner A,Leblanc G,Hatten ME,Heintz N

    更新日期:2003-10-30 00:00:00

  • cMpl ligand is a humoral regulator of megakaryocytopoiesis.

    abstract::Megakaryocytopoiesis is the cellular developmental process that leads to platelet production. At least two humoral growth factors may be necessary for megakaryocyte proliferation and maturation. One is a megakaryocyte-colony stimulating factor (MK-CSF) which induces the proliferation and differentiation of megakaryocy...


    pub_type: 杂志文章


    authors: Wendling F,Maraskovsky E,Debili N,Florindo C,Teepe M,Titeux M,Methia N,Breton-Gorius J,Cosman D,Vainchenker W

    更新日期:1994-06-16 00:00:00

  • Structural plasticity and dynamic selectivity of acid-sensing ion channel-spider toxin complexes.

    abstract::Acid-sensing ion channels (ASICs) are voltage-independent, amiloride-sensitive channels involved in diverse physiological processes ranging from nociception to taste. Despite the importance of ASICs in physiology, we know little about the mechanism of channel activation. Here we show that psalmotoxin activates non-sel...


    pub_type: 杂志文章


    authors: Baconguis I,Gouaux E

    更新日期:2012-09-20 00:00:00