Synthesis and evaluation of cytotoxic activities of artemisinin derivatives.


:Artemisinin is a naturally occurring antimalarial agent which has shown potent anticancer activity. In this work, new artemisinin derivatives with the piperazine group were synthesized. The cytotoxic activities of derivatives 5a-5d were evaluated by MTT assay against ten cell lines. The results showed that 5a-5d were more effective in inhibiting cancer cell growth than artemisinin. 5d was the most active against HepG2 and PLC-PRF-5 cells and presented no cytotoxicity on L-02 cells. Hoechst 33342 staining and flow cytometry experiment revealed that 5d could induce HepG2 and PLC-PRF-5 cell apoptosis. Flow cytometry analysis showed that 5d induced the loss of mitochondrial membrane potential (MMP) and increased the levels of intracellular free calcium and reactive oxygen species. 5d also induced cell cycle arrest in G2/M phase in HepG2 cells. According to the results of Western blotting and caspase-3 kit, 5d could significantly increase the content of p53, bax, Apaf-1, and caspase-3 and decrease the protein level of bcl-2, pro-caspase-9, and pro-caspase-3 in HepG2 cells. These findings indicate that 5d activates the mitochondria-mediated apoptotic pathway in HepG2 cells and may merit further investigation as a potential therapeutic agent for hepatocellular carcinoma.


Chem Biol Drug Des


Sun Q,Wang J,Li Y,Zhuang J,Zhang Q,Sun X,Sun D




Has Abstract


2017-11-01 00:00:00












  • Novel anti-cancer candidates from a combinatorial peptide library.

    abstract::STAT3 is attractive target for development of anti-cancer therapeutics as it is implicated in nearly all forms of human tumors. To identify novel leads, we screened a combinatorial peptide library displayed on the surface of M13 bacteriophage. After three rounds of biopanning, a dodecapeptide with the YYVSWPPDMMHY seq...

    journal_title:Chemical biology & drug design

    pub_type: 杂志文章


    authors: Ambaye ND,Yu HE

    更新日期:2021-01-01 00:00:00

  • Integrating Pharmacophore into Membrane Molecular Dynamics Simulations to Improve Homology Modeling of G Protein-coupled Receptors with Ligand Selectivity: A2A Adenosine Receptor as an Example.

    abstract::Homology modeling has been applied to fill in the gap in experimental G protein-coupled receptors structure determination. However, achievement of G protein-coupled receptors homology models with ligand selectivity remains challenging due to structural diversity of G protein-coupled receptors. In this work, we propose...

    journal_title:Chemical biology & drug design

    pub_type: 杂志文章


    authors: Zeng L,Guan M,Jin H,Liu Z,Zhang L

    更新日期:2015-12-01 00:00:00

  • Current state of a dual behaviour of antimicrobial peptides-Therapeutic agents and promising delivery vectors.

    abstract::Micro-organism resistance is an important challenge in modern medicine due to the global uncontrolled use of antibiotics. Natural and synthetic antimicrobial peptides (AMPs) symbolize a new family of antibiotics, which have stimulated research and clinical interest as new therapeutic options for infections. They repre...

    journal_title:Chemical biology & drug design

    pub_type: 杂志文章,评审


    authors: Piotrowska U,Sobczak M,Oledzka E

    更新日期:2017-12-01 00:00:00

  • Discovery of benzimidazole-based Leishmania mexicana cysteine protease CPB2.8ΔCTE inhibitors as potential therapeutics for leishmaniasis.

    abstract::Chemotherapy is currently the only effective approach to treat all forms of leishmaniasis. However, its effectiveness is severely limited due to high toxicity, long treatment length, drug resistance, or inadequate mode of administration. As a consequence, there is a need to identify new molecular scaffolds and targets...

    journal_title:Chemical biology & drug design

    pub_type: 杂志文章


    authors: De Luca L,Ferro S,Buemi MR,Monforte AM,Gitto R,Schirmeister T,Maes L,Rescifina A,Micale N

    更新日期:2018-09-01 00:00:00

  • Design, Synthesis, and Biological Evaluation of 1-(thiophen-2-yl)-9H-pyrido[3,4-b]indole Derivatives as Anti-HIV-1 Agents.

    abstract::A novel series of 1-(thiophen-2-yl)-9H-pyrido [3,4-b]indole derivatives were synthesized using DL-tryptophan as starting material. All the compounds were characterized by spectral analysis such as (1) H NMR, Mass, IR, elemental analysis and evaluated for inhibitory potency against HIV-1 replication. Among the reported...

    journal_title:Chemical biology & drug design

    pub_type: 杂志文章


    authors: Ashok P,Lu CL,Chander S,Zheng YT,Murugesan S

    更新日期:2015-06-01 00:00:00

  • Design, Synthesis and Biological Evaluation of Two Opioid Agonist and Cav 2.2 Blocker Multitarget Ligands.

    abstract::N-type voltage-dependent Ca(2+) channels (CaV 2.2) are located at nerve endings in the central and peripheral nervous systems and are strongly associated with the pathological processes of cerebral ischaemia and neuropathic pain. CaV 2.2 blockers such as the ω-conotoxin MVIIA (Prialt) are analgesic and have opioid-spa...

    journal_title:Chemical biology & drug design

    pub_type: 杂志文章


    authors: Mollica A,Costante R,Novellino E,Stefanucci A,Pieretti S,Zador F,Samavati R,Borsodi A,Benyhe S,Vetter I,Lewis RJ

    更新日期:2015-08-01 00:00:00

  • Modified benzoxazolone derivative as 18-kDa TSPO ligand.

    abstract::We have synthesized six new congeners of acetamidobenzoxazolone for Translocator Protein [18 kDa, TSPO] imaging. The best in vitro binding affinity (10.8 ± 1.2 nm) for TSPO was found for N-methyl-2-(5-(naphthalen-1-yl)-2-oxobenzo[d]oxazol-3(2H)-yl)-N-phenylacetamide, (NBMP). NBMP was synthesised by Suzuki coupling rea...

    journal_title:Chemical biology & drug design

    pub_type: 杂志文章


    authors: Kumari N,Chadha N,Srivastava P,Mishra LC,Bhagat S,Mishra AK,Tiwari AK

    更新日期:2017-10-01 00:00:00

  • Tumor targeting with 99m Tc radiolabeled peptides: Clinical application and recent development.

    abstract::Targeting overexpressed receptors on the cancer cells with radiolabeled peptides has become very important in nuclear oncology in the recent years. Peptides are small and have easy preparation and easy radiolabeling protocol with no side-effect and toxicity. These properties made them a valuable tool for tumor targeti...

    journal_title:Chemical biology & drug design

    pub_type: 杂志文章,评审


    authors: Rezazadeh F,Sadeghzadeh N

    更新日期:2019-03-01 00:00:00

  • Small steps to new drugs for bugs.

    abstract::Governments, academics and industry are beginning to listen to the medical communities call for new anti-bacterials. This special issue brings together diverse review articles on topics from economics and pricing to new discovery methods. ...

    journal_title:Chemical biology & drug design

    pub_type: 杂志文章


    authors: Melander RJ,Selwood DL

    更新日期:2015-01-01 00:00:00

  • Induction of cell death by a novel naphthoquinone containing a modified anthracycline ring system.

    abstract::The novel naphthoquinone adduct 12,13-Dihydro-N-methyl-6,11,13-trioxo-5H-benzo[4,5]cyclohepta[1,2-b]naphthalen-5,12-imine (hereafter called TU100) was synthesized as a potential chemotherapeutic agent. TU100 arrests tissue culture cells in S and G2/M phases of the cell cycle, followed by rapid induction of apoptosis. ...

    journal_title:Chemical biology & drug design

    pub_type: 杂志文章


    authors: Carvajal D,Kennedy S,Boustani A,Lazar M,Nguyen S,DiCesare JC,Sheaff RJ

    更新日期:2011-11-01 00:00:00

  • Discovering isozyme-selective inhibitor scaffolds of human carbonic anhydrases using structural alignment and de novo drug design approaches.

    abstract::The development of isozyme-selective carbonic anhydrase inhibitors is currently still a great challenge. In the present study, protein-ligand complex structures were obtained by AutoDock Vina with SBR ((R)-N-(3-indol-1-yl-2-methyl-propyl)-4-sulfamoyl-benzamide) as the only inhibitor docked into the binding pockets of ...

    journal_title:Chemical biology & drug design

    pub_type: 杂志文章


    authors: Xiang F,Xiang J,Fang Y,Zhang M,Li M

    更新日期:2014-02-01 00:00:00

  • QSAR models for toxicity of organic substances to Daphnia magna built up by using the CORAL freeware.

    abstract::CORAL (CORrelations And Logic, is a freeware available on the Internet. This freeware is designed to build up quantitative structure - property/activity relationships. The molecular structure for CORAL should be represented by the simplified molecular input line entry system (SMILES). Op...

    journal_title:Chemical biology & drug design

    pub_type: 杂志文章


    authors: Toropova AP,Toropov AA,Benfenati E,Gini G

    更新日期:2012-03-01 00:00:00

  • Synthesis of Met-enkephalin by solution-phase peptide synthesis methodology utilizing para-toluene sulfonic acid as N-terminal masking of l-methionine amino acid.

    abstract::The Met-enkephalin, Tyr-Gly-Gly-Phe-Met, was synthesized by the solution-phase synthesis (SPS) methodology employing -OBzl group as carboxyls' protection, while the t-Boc groups were employed for the N-terminal α-amines' protection for the majority of the amino acids of the pentapeptide sequence. The l-methionine (l-M...

    journal_title:Chemical biology & drug design

    pub_type: 杂志文章


    authors: Khan RA

    更新日期:2016-12-01 00:00:00

  • Minor structural modifications to Pracinostat produce big changes in its biological responses.

    abstract::A series of compounds similar to Pracinostat that contained benzimidazole ring and N-hydroxyacrylamide attached at 5- or 6-position were designed, synthesized, and evaluated as HDAC inhibitors. It was interesting to find that the corresponding derivative 1 with N-hydroxyacrylamide attached at 5-position was a potent H...

    journal_title:Chemical biology & drug design

    pub_type: 杂志文章


    authors: Jia R,Sun P,Zhang Y,Ge Y,Yu N

    更新日期:2019-08-01 00:00:00

  • Fullerene isoniazid conjugate--a tuberculostat with increased lipophilicity: synthesis and evaluation of antimycobacterial activity.

    abstract::A fullerene-isoniazid conjugate has been synthesized by 1, 3 dipolar cycloaddition reaction of fullerene (C(60)) with isonicotinic acid (4-formyl-benzylidene) hydrazide and N-methylglycine. The identity and purity of the compound was confirmed by elemental analysis, (1)H NMR, (13)C NMR and MALDI-TOF mass spectral anal...

    journal_title:Chemical biology & drug design

    pub_type: 信件


    authors: Kumar A,Patel G,Menon SK

    更新日期:2009-05-01 00:00:00

  • Preparation, characterization, and in vitro evaluation of isoniazid and rifampicin-loaded archaeosomes.

    abstract::The ability of Archaea to adapt their membrane lipid compositions to extreme environments has brought in archaeosomes into consideration for the development of drug delivery systems overcoming the physical, biological blockades that the body exhibits against drug therapies. In this study, we prepared unilamellar archa...

    journal_title:Chemical biology & drug design

    pub_type: 杂志文章


    authors: Attar A,Bakir C,Yuce-Dursun B,Demir S,Cakmakci E,Danis O,Birbir M,Ogan A

    更新日期:2018-01-01 00:00:00

  • The Binding Mode Prediction and Similar Ligand Potency in the Active Site of Vitamin D Receptor with QM/MM Interaction, MESP, and MD Simulation.

    abstract::Non-secosteroidal ligands are well-known vitamin D receptor (VDR) agonists. In this study, we described a combined QM/MM to define the protein-ligand interaction energy a strong positive correlation in both QM-MM interaction energy and binding free energy against the biological activity. The molecular dynamics simulat...

    journal_title:Chemical biology & drug design

    pub_type: 杂志文章


    authors: Selvaraman N,Selvam SK,Muthusamy K

    更新日期:2016-08-01 00:00:00

  • Structural models and binding site prediction of the C-terminal domain of human Hsp90: a new target for anticancer drugs.

    abstract::Heat shock protein 90 is a valuable target for anticancer drugs because of its role in the activation and stabilization of multiple oncogenic signalling proteins. While several compounds inhibit heat shock protein 90 by binding the N-terminal domain, recent studies have proved that the C-terminal domain is important f...

    journal_title:Chemical biology & drug design

    pub_type: 杂志文章


    authors: Sgobba M,Degliesposti G,Ferrari AM,Rastelli G

    更新日期:2008-05-01 00:00:00

  • KinomeRun: An interactive utility for kinome target screening and interaction fingerprint analysis towards holistic visualization on kinome tree.

    abstract::Kinases are key targets for many of the pathological conditions. Inverse screening of ligands serves as an essential mode to identify potential kinase targets in modern drug discovery research. Hence, we intend to develop KinomeRun, a robust pipeline for inverse screening and kinome tree visualization through the seam...

    journal_title:Chemical biology & drug design

    pub_type: 杂志文章


    authors: Ansar S,Vetrivel U

    更新日期:2020-10-01 00:00:00

  • Design, synthesis, and in vitro antitumor activity of a transferrin receptor-targeted peptide-doxorubicin conjugate.

    abstract::In this study, a peptide-drug conjugate was designed and synthesized by connecting a transferrin receptor (TfR)-targeted binding peptide analog BP9a (CAHLHNRS) with doxorubicin (DOX) through N-succinimidyl-3-maleimidopropionate (SMP) as the cross-linker. Confocal laser scanning microscopy results indicated that free D...

    journal_title:Chemical biology & drug design

    pub_type: 杂志文章


    authors: Li S,Zhao H,Fan Y,Zhao G,Wang R,Wen F,Wang J,Wang X,Wang Y,Gao Y

    更新日期:2020-01-01 00:00:00

  • Identifying de-NEDDylation inhibitors: Virtual high-throughput screens targeting SENP8.

    abstract::Protein modification can have far-reaching effects. NEDDylation, a protein modification process with the protein NEDD8, stabilizes and modifies how the targeted protein interacts with other proteins. Its role in system regulation makes it a prime therapeutic target, and virtual high-throughput screening has already id...

    journal_title:Chemical biology & drug design

    pub_type: 杂志文章


    authors: Chen JJ,Schmucker LN,Visco DP Jr

    更新日期:2019-04-01 00:00:00

  • Serendipitous discovery of a prodrug of a PARP-1 inhibitor.

    abstract::During SAR development of previously reported pyrrolocarbazole 1, a potent PARP-1 inhibitor, compound 14, was discovered serendipitously to be a prodrug of compound 1. ...

    journal_title:Chemical biology & drug design

    pub_type: 信件


    authors: Dunn D,Husten J,Aimone LD,Ator MA,Chatterjee S

    更新日期:2013-09-01 00:00:00

  • Probing the Binding Pocket of the Broadly Tuned Human Bitter Taste Receptor TAS2R14 by Chemical Modification of Cognate Agonists.

    abstract::Sensing potentially harmful bitter substances in the oral cavity is achieved by a group of (˜) 25 receptors, named TAS2Rs, which are expressed in specialized sensory cells and recognize individual but overlapping sets of bitter compounds. The receptors differ in their tuning breadths ranging from narrowly to broadly t...

    journal_title:Chemical biology & drug design

    pub_type: 杂志文章


    authors: Karaman R,Nowak S,Di Pizio A,Kitaneh H,Abu-Jaish A,Meyerhof W,Niv MY,Behrens M

    更新日期:2016-07-01 00:00:00

  • Synthesis of ranolazine derivatives containing the (1S,4S)-2,5-diazabicyclo[2.2.1]heptane moiety and their evaluation as vasodilating agents.

    abstract::Two diazabicyclic analogues of ranolazine, (S,S,S)-5 and (S,S,R)-5, and their epimeric mixture were synthesized. Furthermore, their vasomotor effects on rat aorta rings precontracted with phenylephrine were analyzed. These compounds showed vasodilating effects significantly greater than ranolazine. The vasodilating ac...

    journal_title:Chemical biology & drug design

    pub_type: 杂志文章


    authors: López-Ortiz M,Monsalvo I,Demare P,Paredes C,Mascher D,Hernández C,Hernández M,Regla I

    更新日期:2014-06-01 00:00:00

  • Mapping ligand-receptor interfaces: approaching the resolution limit of benzophenone-based photoaffinity scanning.

    abstract::Photoaffinity crosslinking has yielded important insights in the study of G protein-coupled receptors and the mode of ligand binding. The most widely used photolabile moiety is p-benzoylphenylalanine largely because of its reportedly high site specificity, reduced reactivity to water and light, photokinetics, and ease...

    journal_title:Chemical biology & drug design

    pub_type: 信件


    authors: Wittelsberger A,Mierke DF,Rosenblatt M

    更新日期:2008-04-01 00:00:00

  • Design of peptidomimetics for inhibition of HER2 receptor dimerization by a combination of virtual screening, MD simulations, and QSAR in silico methods.

    abstract::Malfunction or overexpression of ErbB receptors (epidermal growth factor receptors) is associated with occurrence and severity of several types of cancer. Initiation of signal cascades by ErbB2 (also known as human epidermal growth factor receptor 2/neu) in breast cancer has been blocked by monoclonal antibodies such ...

    journal_title:Chemical biology & drug design

    pub_type: 杂志文章


    authors: Jamalan M,Zeinali M,Barzegari Asadabadi E

    更新日期:2013-04-01 00:00:00

  • Sulfated small molecules targeting eBV in Burkitt lymphoma: from in silico screening to the evidence of in vitro effect on viral episomal DNA.

    abstract::Epstein-Barr virus (EBV) infects more than 90% of the world population. Following primary infection, Epstein-Barr virus persists in an asymptomatic latent state. Occasionally, it may switch to lytic infection. Latent EBV infection has been associated with several diseases, such as Burkitt lymphoma (BL). To date, there...

    journal_title:Chemical biology & drug design

    pub_type: 杂志文章


    authors: Lima RT,Seca H,Palmeira A,Fernandes MX,Castro F,Correia-da-Silva M,Nascimento MS,Sousa E,Pinto M,Vasconcelos MH

    更新日期:2013-05-01 00:00:00

  • Development of broad-spectrum halomethyl ketone inhibitors against coronavirus main protease 3CL(pro).

    abstract::Coronaviruses comprise a large group of RNA viruses with diverse host specificity. The emergence of highly pathogenic strains like the SARS coronavirus (SARS-CoV), and the discovery of two new coronaviruses, NL-63 and HKU1, corroborates the high rate of mutation and recombination that have enabled them to cross specie...

    journal_title:Chemical biology & drug design

    pub_type: 杂志文章


    authors: Bacha U,Barrila J,Gabelli SB,Kiso Y,Mario Amzel L,Freire E

    更新日期:2008-07-01 00:00:00

  • Design, Synthesis and in vivo Evaluation of Novel C-Aryl Glucosides as Potent Sodium-Dependent Glucose Cotransporters Inhibitors for the Treatment of Diabetes.

    abstract::A series of novel C-aryl glucosides with substituents at the 3'-position or cyclization at 3', 4'-positions of the distal aryl ring were designed and synthesized, which might decrease the oxidative metabolism of dapagliflozin. Preliminary evaluation for hypoglycemic effect and the risk of hypoglycemia were carried out...

    journal_title:Chemical biology & drug design

    pub_type: 杂志文章


    authors: Li Z,Wang X,Xu X,Qiu Q,Jiao L,Huang W,Qian H

    更新日期:2015-10-01 00:00:00

  • AVCpred: an integrated web server for prediction and design of antiviral compounds.

    abstract::Viral infections constantly jeopardize the global public health due to lack of effective antiviral therapeutics. Therefore, there is an imperative need to speed up the drug discovery process to identify novel and efficient drug candidates. In this study, we have developed quantitative structure-activity relationship (...

    journal_title:Chemical biology & drug design

    pub_type: 杂志文章


    authors: Qureshi A,Kaur G,Kumar M

    更新日期:2017-01-01 00:00:00