Abstract:
:This study explored whether valproic acid (VPA, a histone deacetylase inhibitor) could radiosensitize osteosarcoma and primary-culture tumor cells, and determined the mechanism of VPA-induced radiosensitization. The working system included osteosarcoma cells (U2OS) and primary-culture cells from chemical carcinogen (DMBA)-induced breast cancer in rats; and clonogenic survival, immunofluorescence, fluorescent in situ hybridization (FISH) for chromosome aberrations, and comet assays were used in this study. It was found that VPA at the safe or critical safe concentration of 0.5 or 1.0 mM VPA could result in the accumulation of more ionizing radiation (IR)-induced DNA double strand breaks, and increase the cell radiosensitivity. VPA-induced radiosensitivity was associated with the inhibition of DNA repair activity in the working systems. In addition, the chromosome aberrations including chromosome breaks, chromatid breaks, and radial structures significantly increased after the combination treatment of VPA and IR. Importantly, the results obtained by primary-culture cells from the tissue of chemical carcinogen-induced breast cancer in rats further confirmed our findings. The data in this study demonstrated that VPA at a safe dose was a radiosensitizer for osteosarcoma and primary-culture tumor cells through suppressing DNA-double strand breaks repair function.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Liu G,Wang H,Zhang F,Tian Y,Tian Z,Cai Z,Lim D,Feng Zdoi
10.3390/ijms18051027subject
Has Abstractpub_date
2017-05-10 00:00:00issue
5issn
1422-0067pii
ijms18051027journal_volume
18pub_type
杂志文章abstract::Atherosclerosis is an inflammatory disease in which dysfunction of mitochondria play an important role, and disorders of lipid management intensify this process. Agmatine, an endogenous polyamine formed by decarboxylation of arginine, exerts a protective effect on mitochondria and modulates fatty acid metabolism. We i...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms18081706
更新日期:2017-08-04 00:00:00
abstract::Human Ribonuclease 6 is a secreted protein belonging to the ribonuclease A (RNaseA) superfamily, a vertebrate specific family suggested to arise with an ancestral host defense role. Tissue distribution analysis revealed its expression in innate cell types, showing abundance in monocytes and neutrophils. Recent evidenc...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms17040552
更新日期:2016-04-13 00:00:00
abstract::Alterations to the programmed cell death protein-1 (PD-1) pathway were previously shown to be involved in a poorer prognosis for patients with proliferative glomerulonephritis (PGN). Here, we investigated the association between several infectious agents and the expression of PD-1 and its ligand (PD-L1) on T and B lym...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21218001
更新日期:2020-10-27 00:00:00
abstract::In the present study, we evaluated tumor-infiltrating lymphocytes (TILs) and blood regulatory T lymphocyte (Tregs, CD4+/CD25+/FoxP3+) expression in bladder cancer patients. The number of CD4+, CD8+, CD25+, FoxP3+ and CD20+ TILs was analyzed in association with clinico-pathomorphological features. In more advanced meta...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms17030285
更新日期:2016-02-25 00:00:00
abstract::Specific labelling of target cell surfaces using antibody-conjugated paramagnetic nanobeads is essential for efficient magnetic cell separation. However, studies examining parameters determining the kinetics of bead-cell binding are scarce. The present study determines the binding rates for specific and unspecific bin...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms15058821
更新日期:2014-05-19 00:00:00
abstract::In advanced cirrhosis, the TNFα-mediated intestinal inflammation and bacteria dysbiosis are involved in the development of inflammation and vasoconstriction-related renal dysfunction. In colitis and acute kidney injury models, activation of SIRT1 attenuates the TNFα-mediated intestinal and renal abnormalities. This st...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms22031233
更新日期:2021-01-27 00:00:00
abstract::Adoptive cellular immunotherapy (ACI) is a promising treatment for a number of cancers. Cytokine-induced killer cells (CIKs) are considered to be major cytotoxic immunologic effector cells. Usually cancer cells are able to suppress antitumor responses by secreting immunosuppressive factors. CIKs have significant antit...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms20174307
更新日期:2019-09-03 00:00:00
abstract::This review focuses on the role of 5'-activated protein kinase (AMPK) in the effects of resveratrol (RSV) and some RSV derivatives on hepatic steatosis. In vitro studies, performed in different hepatic cell models, have demonstrated that RSV is effective in preventing liver TG accumulation by activating AMPK, due to i...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms19113473
更新日期:2018-11-05 00:00:00
abstract::Members of the placenta-specific miRNA cluster C19MC, including miR-519d, are secreted by fetal trophoblast cells within extracellular vesicles (EVs). Trophoblast-derived EVs can be internalized by the autologous trophoblast and surrounding maternal immune cells, resulting in coordination of cellular responses. The st...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21103458
更新日期:2020-05-14 00:00:00
abstract::It was reported that substance P had beneficial effects in the healing of acute tendon injury. However, the relationship between substance P and degenerative tendinopathy development remains unclear. The purpose of this study was to determine the role of substance P in the pathogenesis of tendinopathy. Healthy and ten...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms18061241
更新日期:2017-06-09 00:00:00
abstract::Pterostilbene (PTE) is a natural sterbenoid contained in blueberries that has an antioxidant effect. In contrast, PTE also generates oxidative stress in cancer cells and provides an antitumor effect. Here, we examined the potential mechanism of this contrasting effect of PTE using three gastrointestinal cancer cell li...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21249347
更新日期:2020-12-08 00:00:00
abstract::Burkholderia cenocepacia is an important opportunistic pathogen in cystic fibrosis (CF) patients, and has also been isolated from natural environments. In previous work, we explored the virulence and pathogenic potential of environmental B. cenocepacia strains and demonstrated that they do not differ from clinical str...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms18112417
更新日期:2017-11-14 00:00:00
abstract::Caspase-8 was originally identified as a central player of programmed cell death triggered by death receptor stimulation. In that context, its activity is tightly regulated through several mechanisms, with the best established being the expression of FLICE-like inhibitory protein (FLIP) family proteins and the Src-dep...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms19123798
更新日期:2018-11-29 00:00:00
abstract::This Special Issue of International Journal of Molecular Sciences (IJMS) contains 7 reviews and 12 original research papers written by a panel of experts who highlight recent advances in molecular structure and cellular function of purinergic P2 receptors[...]. ...
journal_title:International journal of molecular sciences
pub_type: 社论
doi:10.3390/ijms22010383
更新日期:2020-12-31 00:00:00
abstract::Overexpression of epidermal growth factor receptors (EGFR) occurs in >90% of pancreatic ductal adenocarcinomas (PDACs) and is associated with a poorer prognosis. A systematic review of electronic databases identified studies exploring the addition of EGFR-targeted treatment to chemotherapy in patients with locally adv...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,meta分析,评审
doi:10.3390/ijms18050909
更新日期:2017-04-26 00:00:00
abstract::α-Synuclein is a naturally unfolded protein which easily aggregates and forms toxic inclusions and deposits. It is associated with several neurodegenerative diseases, including Parkinson's disease (PD), dementia with Lewy bodies (DLB), and multiple system atrophy (MSA). These diseases, called synucleinopathies, have o...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21082801
更新日期:2020-04-17 00:00:00
abstract::CD155 is an adhesion molecule belonging to the Nectin/Nectin-like family often overexpressed on tumor cells and involved in many different processes such as cell adhesion, migration and proliferation. In contrast to these pro-tumorigenic functions, CD155 is also a ligand for the activating receptor DNAM-1 expressed on...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21030922
更新日期:2020-01-30 00:00:00
abstract::Chronic inflammation in adipose tissue may contribute to depot-specific adipose tissue expansion, leading to obesity and insulin resistance. Dietary supplementation with quercetin or botanical extracts containing quercetin attenuates high fat diet (HFD)-induced obesity and insulin resistance and decreases inflammation...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19030895
更新日期:2018-03-17 00:00:00
abstract::This study examined whether the conserved arginine cluster present within the 29-amino acid insert of the long form of the D₂ dopamine receptor (D2L-R) confers its predominant intracellular localization. We hypothesized that the conserved arginine cluster (RRR) located within the insert could act as an RXR-type endopl...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms17071152
更新日期:2016-07-19 00:00:00
abstract::CXCL7, a chemokine highly expressed in platelets, orchestrates neutrophil recruitment during thrombosis and related pathophysiological processes by interacting with CXCR2 receptor and sulfated glycosaminoglycans (GAG). CXCL7 exists as monomers and dimers, and dimerization (~50 μM) and CXCR2 binding (~10 nM) constants ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms18030508
更新日期:2017-02-26 00:00:00
abstract::A novel application for non-thermal plasma is the induction of immunogenic cancer cell death for cancer immunotherapy. Cells undergoing immunogenic death emit danger signals which facilitate anti-tumor immune responses. Although pathways leading to immunogenic cell death are not fully understood; oxidative stress is c...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms18050966
更新日期:2017-05-03 00:00:00
abstract:BACKGROUND:Psoriasis is hallmarked by vascular dysfunction, arterial hypertension, and an increased risk for cardiovascular diseases. We have shown recently that skin-driven interleukin (IL)-17A expression promotes psoriasis-like disease in mice, and this is associated with vascular inflammation, vascular dysfunction, ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20174261
更新日期:2019-08-30 00:00:00
abstract::Ile-Ala-Val-Pro-Gly-Glu-Val-Ala (IAVPGEVA), Ile-Ala-Val-Pro-Thr-Gly-Val-Ala (IAVPTGVA) and Leu-Pro-Tyr-Pro (LPYP), three peptides deriving from soy glycinin hydrolysis, are known to regulate cholesterol metabolism in human hepatic HepG2 cells. We have recently demonstrated that the mechanism of action involves the act...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms161126029
更新日期:2015-11-16 00:00:00
abstract::Human MLH3 (hMLH3) gene has been suggested to play a role in the DNA mismatch repair mechanism, while it may also be associated with abnormal spermatogenesis and subsequently male infertility. The aim of the present study was to investigate possible relationships between the single nucleotide polymorphism (SNP) rs1750...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms18020314
更新日期:2017-02-02 00:00:00
abstract::Long-chain fatty acyl-CoA synthetase (ACSLs) is an essential enzyme for the synthesis of fatty acyl-CoA. ACSL1 plays a key role in the synthesis of triglycerides, phospholipids, and cholesterol esters. BACKGROUND:In the current study, triglyceride content did not increase after overexpression of the ACSL1 gene. METH...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21062044
更新日期:2020-03-17 00:00:00
abstract::Growth factors and other agents that could potentially enhance tissue regeneration have been identified, but their therapeutic value in clinical medicine has been limited for reasons such as difficulty to maintain bioactivity of locally applied therapeutics in the protease-rich environment of regenerating tissues. Alt...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms161023556
更新日期:2015-09-30 00:00:00
abstract::When cellular reducing enzymes fail to shield the cell from increased amounts of reactive oxygen species (ROS), oxidative stress arises. The redox state is misbalanced, DNA and proteins are damaged and cellular transcription networks are activated. This condition can lead to the initiation and/or to the progression of...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms131013104
更新日期:2012-10-12 00:00:00
abstract::Growing evidence suggests that inflammatory responses, in both the brain and peripheral tissues, contribute to disease pathology in Huntington's disease (HD), an inherited, progressive neurodegenerative disorder typically affecting adults in their 30-40 s. Hence, studies of inflammation-related markers in peripheral f...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21176363
更新日期:2020-09-02 00:00:00
abstract::Plant regeneration and somatic embryogenesis through interspecific hybridization among different Carica species were studied for the development of a papaya ringspot virus-resistant variety. The maximum fruit sets were recorded from the cross of the native variety C. papaya cv. Shahi with the wild species C. cauliflor...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms131217065
更新日期:2012-12-12 00:00:00
abstract::Mitochondria have emerged as key players regarding cellular homeostasis not only due to their contribution regarding energy production through oxidative phosphorylation, but also due to their involvement in signaling, ion regulation, and programmed cell death. Indeed, current knowledge supports the notion that mitocho...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms18081821
更新日期:2017-08-22 00:00:00