The Effect of VPA on Increasing Radiosensitivity in Osteosarcoma Cells and Primary-Culture Cells from Chemical Carcinogen-Induced Breast Cancer in Rats.


:This study explored whether valproic acid (VPA, a histone deacetylase inhibitor) could radiosensitize osteosarcoma and primary-culture tumor cells, and determined the mechanism of VPA-induced radiosensitization. The working system included osteosarcoma cells (U2OS) and primary-culture cells from chemical carcinogen (DMBA)-induced breast cancer in rats; and clonogenic survival, immunofluorescence, fluorescent in situ hybridization (FISH) for chromosome aberrations, and comet assays were used in this study. It was found that VPA at the safe or critical safe concentration of 0.5 or 1.0 mM VPA could result in the accumulation of more ionizing radiation (IR)-induced DNA double strand breaks, and increase the cell radiosensitivity. VPA-induced radiosensitivity was associated with the inhibition of DNA repair activity in the working systems. In addition, the chromosome aberrations including chromosome breaks, chromatid breaks, and radial structures significantly increased after the combination treatment of VPA and IR. Importantly, the results obtained by primary-culture cells from the tissue of chemical carcinogen-induced breast cancer in rats further confirmed our findings. The data in this study demonstrated that VPA at a safe dose was a radiosensitizer for osteosarcoma and primary-culture tumor cells through suppressing DNA-double strand breaks repair function.


Int J Mol Sci


Liu G,Wang H,Zhang F,Tian Y,Tian Z,Cai Z,Lim D,Feng Z




Has Abstract


2017-05-10 00:00:00










  • Effects of Different Pollens on Primary Metabolism and Lignin Biosynthesis in Pear.

    abstract::To investigate the effect of pollination on the fruit quality of 'Dangshan Su' pear, 'Dangshan Su' was fertilized by the pollen of 'Wonhwang' (Pyrus pyrifolia Nakai.) (DW) and 'Jingbaili' (Pyrus ussuriensis Maxim.) (DJ). The analysis of primary metabolites was achieved through untargeted metabolomics, and the quantita...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Li S,Su X,Abdullah M,Sun Y,Li G,Cheng X,Lin Y,Cai Y,Jin Q

    更新日期:2018-08-02 00:00:00

  • Androgen receptor phosphorylation at serine 308 and serine 791 predicts enhanced survival in castrate resistant prostate cancer patients.

    abstract::We previously reported that AR phosphorylation at serine 213 was associated with poor outcome and may contribute to prostate cancer development and progression. This study investigates if specific AR phosphorylation sites have differing roles in the progression of hormone naïve prostate cancer (HNPC) to castrate resis...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: McCall P,Adams CE,Willder JM,Bennett L,Qayyum T,Orange C,Underwood MA,Edwards J

    更新日期:2013-08-13 00:00:00

  • Repellent and Antifeedant Activities of Citral-Derived Lactones against the Peach Potato Aphid.

    abstract::Citral is well known for its antimicrobial, antifungal, and insecticidal activities. Natural sesquiterpene α-methylenelactones also exhibit a broad spectrum of biological activities. The aim of the study was to explore the effect of structural changes to citral molecules on citral behavior-modifying activity towards M...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Dancewicz K,Szumny A,Wawrzeńczyk C,Gabryś B

    更新日期:2020-10-28 00:00:00

  • Optimizing a Male Reproductive Aging Mouse Model by D-Galactose Injection.

    abstract::The d-galactose (d-gal)-injected animal model, which is typically established by administering consecutive subcutaneous d-gal injections to animals for approximately six or eight weeks, has been frequently used for aging research. In addition, this animal model has been demonstrated to accelerate aging in the brain, k...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Liao CH,Chen BH,Chiang HS,Chen CW,Chen MF,Ke CC,Wang YY,Lin WN,Wang CC,Lin YH

    更新日期:2016-01-13 00:00:00

  • Hexokinase-II Inhibition Synergistically Augments the Anti-tumor Efficacy of Sorafenib in Hepatocellular Carcinoma.

    abstract::This study aimed to examine whether inhibition of hexokinase (HK)-II activity enhances the efficacy of sorafenib in in-vivo models of hepatocellular carcinoma (HCC), and to evaluate the prognostic implication of HK-II expression in patients with HCC. We used 3-bromopyruvate (3-BP), a HK-II inhibitor to target HK-II. T...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Yoo JJ,Yu SJ,Na J,Kim K,Cho YY,Lee YB,Cho EJ,Lee JH,Kim YJ,Youn H,Yoon JH

    更新日期:2019-03-14 00:00:00

  • STAT3 in Tumor-Associated Myeloid Cells: Multitasking to Disrupt Immunity.

    abstract::Myeloid immune cells, such as dendritic cells, monocytes, and macrophages, play a central role in the generation of immune responses and thus are often either disabled or even hijacked by tumors. These new tolerogenic activities of tumor-associated myeloid cells are controlled by an oncogenic transcription factor, sig...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Su YL,Banerjee S,White SV,Kortylewski M

    更新日期:2018-06-19 00:00:00

  • Mg2+ Is a Missing Link in Plant Cell Ca2+ Signalling and Homeostasis-A Study on Vicia faba Guard Cells.

    abstract::Hyperpolarization-activated calcium channels (HACCs) are found in the plasma membrane and tonoplast of many plant cell types, where they have an important role in Ca2+-dependent signalling. The unusual gating properties of HACCs in plants, i.e., activation by membrane hyperpolarization rather than depolarization, dict...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Lemtiri-Chlieh F,Arold ST,Gehring C

    更新日期:2020-05-27 00:00:00

  • Aquaporin Channels in the Heart-Physiology and Pathophysiology.

    abstract::Mammalian aquaporins (AQPs) are transmembrane channels expressed in a large variety of cells and tissues throughout the body. They are known as water channels, but they also facilitate the transport of small solutes, gasses, and monovalent cations. To date, 13 different AQPs, encoded by the genes AQP0-AQP12, have been...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Verkerk AO,Lodder EM,Wilders R

    更新日期:2019-04-25 00:00:00

  • Environmental risk factors for multiple sclerosis: a review with a focus on molecular mechanisms.

    abstract::Multiple sclerosis (MS) is a chronic disabling disease of the central nervous system commonly affecting young adults. Pathologically, there are patches of inflammation (plaques) with demyelination of axons and oligodendrocyte loss. There is a global latitude gradient in MS prevalence, and incidence of MS is increasing...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: O'Gorman C,Lucas R,Taylor B

    更新日期:2012-01-01 00:00:00

  • TNF-α gene knockout in triple negative breast cancer cell line induces apoptosis.

    abstract::Tumor necrosis factor alpha (TNF-α) is a pro-inflammatory cytokine involved in the promotion and progression of cancer, including triple negative breast cancer cells. Thus, there is significant interest in understanding the molecular signaling pathways that connect TNF-α with the survival of tumor cells. In our experi...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Pileczki V,Braicu C,Gherman CD,Berindan-Neagoe I

    更新日期:2012-12-24 00:00:00

  • Isoliquiritigenin Induces Autophagy and Inhibits Ovarian Cancer Cell Growth.

    abstract::Ovarian cancer is one of the commonest gynecologic malignancies, which has a poor prognosis for patients at the advanced stage. Isoliquiritigenin (ISL), an active flavonoid component of the licorice plant, previously demonstrated antioxidant, anti-inflammatory, and tumor suppressive effects. In this study, we investig...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Chen HY,Huang TC,Shieh TM,Wu CH,Lin LC,Hsia SM

    更新日期:2017-09-21 00:00:00

  • Using Proteomics to Understand How Leishmania Parasites Survive inside the Host and Establish Infection.

    abstract::Leishmania is a protozoan parasite that causes a wide range of different clinical manifestations in mammalian hosts. It is a major public health risk on different continents and represents one of the most important neglected diseases. Due to the high toxicity of the drugs currently used, and in the light of increasing...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Veras PS,Bezerra de Menezes JP

    更新日期:2016-08-19 00:00:00

  • Myricanol induces apoptotic cell death and anti-tumor activity in non-small cell lung carcinoma in vivo.

    abstract::This study explored the inhibiting effect and mechanism of myricanol on lung adenocarcinoma A549 xenografts in nude mice. Forty nude mice with subcutaneous A549 xenografts were randomly divided into five groups: high-dose myricanol (40 mg/kg body weight) group; middle-dose myricanol (20 mg/kg body weight) group; low-d...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Dai G,Tong Y,Chen X,Ren Z,Ying X,Yang F,Chai K

    更新日期:2015-01-26 00:00:00

  • The Endocannabinoid System and Autism Spectrum Disorders: Insights from Animal Models.

    abstract::Autism spectrum disorder (ASD) defines a group of neurodevelopmental disorders whose symptoms include impaired communication and social interaction with restricted or repetitive motor movements, frequently associated with general cognitive deficits. Although it is among the most severe chronic childhood disorders in t...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Zamberletti E,Gabaglio M,Parolaro D

    更新日期:2017-09-07 00:00:00

  • Biogenesis and mechanism of action of small non-coding RNAs: insights from the point of view of structural biology.

    abstract::Non-coding RNAs are dominant in the genomic output of the higher organisms being not simply occasional transcripts with idiosyncratic functions, but constituting an extensive regulatory network. Among all the species of non-coding RNAs, small non-coding RNAs (miRNAs, siRNAs and piRNAs) have been shown to be in the cor...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Costa MC,Leitão AL,Enguita FJ

    更新日期:2012-01-01 00:00:00

  • Integrated Molecular Characterization of Gastrointestinal Stromal Tumors (GIST) Harboring the Rare D842V Mutation in PDGFRA Gene.

    abstract::Gastrointestinal stromal tumors (GIST) carrying the D842V activating mutation in the platelet-derived growth factor receptor alpha (PDGFRA) gene are a very rare subgroup of GIST (about 10%) known to be resistant to conventional tyrosine kinase inhibitors (TKIs) and to show an indolent behavior. In this study, we perfo...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Indio V,Astolfi A,Tarantino G,Urbini M,Patterson J,Nannini M,Saponara M,Gatto L,Santini D,do Valle IF,Castellani G,Remondini D,Fiorentino M,von Mehren M,Brandi G,Biasco G,Heinrich MC,Pantaleo MA

    更新日期:2018-03-04 00:00:00

  • Unraveling the Role of Inflammation in the Pathogenesis of Diabetic Kidney Disease.

    abstract::Diabetic kidney disease (DKD) remains the leading cause of end-stage renal disease (ESRD) and is therefore a major burden on the healthcare system. Patients with DKD are highly susceptible to developing cardiovascular disease, which contributes to increased morbidity and mortality rates. While progress has been made t...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Matoba K,Takeda Y,Nagai Y,Kawanami D,Utsunomiya K,Nishimura R

    更新日期:2019-07-10 00:00:00

  • The Implication of Substance P in the Development of Tendinopathy: A Case Control Study.

    abstract::It was reported that substance P had beneficial effects in the healing of acute tendon injury. However, the relationship between substance P and degenerative tendinopathy development remains unclear. The purpose of this study was to determine the role of substance P in the pathogenesis of tendinopathy. Healthy and ten...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Han SH,Choi W,Song J,Kim J,Lee S,Choi Y,Byun SE,Ahn T,Ahn H,Ding C,Baik L,Ward S,Ting K,Lee S

    更新日期:2017-06-09 00:00:00

  • Deleterious Effect of Advanced CKD on Glyoxalase System Activity not Limited to Diabetes Aetiology.

    abstract::Methylglyoxal production is increased in diabetes. Methylglyoxal is efficiently detoxified by enzyme glyoxalase 1 (GLO1). The aim was to study the effect of diabetic and CKD milieu on (a) GLO1 gene expression in peripheral blood mononuclear cells; (b) GLO1 protein levels in whole blood; and (c) GLO1 activity in RBCs i...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Pácal L,Chalásová K,Pleskačová A,Řehořová J,Tomandl J,Kaňková K

    更新日期:2018-05-18 00:00:00

  • Proteomic analysis identified DJ-1 as a cisplatin resistant marker in non-small cell lung cancer.

    abstract::The aim of study is to identify cisplatin-resistance associated biomarkers for non-small cell lung cancers (NSCLC). We use two-dimensional electrophoresis (2-DE) combined with MALDI-TOF mass spectrometry to compare the proteome between lung cancer cell line A549 and its cisplatin-resistant subline A549/DDP. Nine cispl...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Zeng HZ,Qu YQ,Zhang WJ,Xiu B,Deng AM,Liang AB

    更新日期:2011-01-01 00:00:00

  • Bevacizumab-Based Chemotherapy Combined with Regional Deep Capacitive Hyperthermia in Metastatic Cancer Patients: A Pilot Study.

    abstract::As an angiogenesis inhibitor, bevacizumab has been investigated in combination with different chemotherapeutic agents, achieving an established role for metastatic cancer treatment. However, potential synergic anti-angiogenic effects of hyperthermia have not tested to date in literature. The aim of our study was to an...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Ranieri G,Ferrari C,Di Palo A,Marech I,Porcelli M,Falagario G,Ritrovato F,Ramunni L,Fanelli M,Rubini G,Gadaleta CD

    更新日期:2017-07-06 00:00:00

  • Chromosomal Density of Cancer Up-Regulated Genes, Aberrant Enhancer Activity and Cancer Fitness Genes Are Associated with Transcriptional Cis-Effects of Broad Copy Number Gains in Colorectal Cancer.

    abstract::Broad Copy Number Gains (BCNGs) are copy-number increases of chromosomes or large segments of chromosomal arms. Publicly-available single-nucleotide polymorphism (SNP) array and RNA-Seq data of colon adenocarcinoma (COAD) samples from The Cancer Genome Atlas (TCGA) consortium allowed us to design better control groups...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Condorelli DF,Privitera AP,Barresi V

    更新日期:2019-09-19 00:00:00

  • Ligand Shaping in Induced Fit Docking of MraY Inhibitors. Polynomial Discriminant and Laplacian Operator as Biological Activity Descriptors.

    abstract::Docking-i.e., interaction of a small molecule (ligand) with a proteic structure (receptor)-represents the ground of drug action mechanism of the vast majority of bioactive chemicals. Ligand and receptor accommodate their geometry and energy, within this interaction, in the benefit of receptor-ligand complex. In an ind...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Lungu CN,Diudea MV,Putz MV

    更新日期:2017-06-27 00:00:00

  • A Novel Tetraenoic Fatty Acid Isolated from Amaranthus spinosus Inhibits Proliferation and Induces Apoptosis of Human Liver Cancer Cells.

    abstract::Amaranthus spinosus Linn. (Family: Amaranthaceae) has been shown to be useful in preventing and mitigating adverse pathophysiological conditions and complex diseases. However, only limited information is available on the anticancer potential of this plant. In this study, we examined the antiproliferative and pro-apopt...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Mondal A,Guria T,Maity TK,Bishayee A

    更新日期:2016-09-22 00:00:00

  • The Effect of Alendronate Loaded Biphasic Calcium Phosphate Scaffolds on Bone Regeneration in a Rat Tibial Defect Model.

    abstract::This study investigated the effect of alendronate (Aln) released from biphasic calcium phosphate (BCP) scaffolds. We evaluated the in vitro osteogenic differentiation of Aln/BCP scaffolds using MG-63 cells and the in vivo bone regenerative capability of Aln/BCP scaffolds using a rat tibial defect model with radiograph...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Park KW,Yun YP,Kim SE,Song HR

    更新日期:2015-11-06 00:00:00

  • Contributions of Myosin Light Chain Kinase to Regulation of Epithelial Paracellular Permeability and Mucosal Homeostasis.

    abstract::Intestinal barrier function is required for the maintenance of mucosal homeostasis. Barrier dysfunction is thought to promote progression of both intestinal and systemic diseases. In many cases, this barrier loss reflects increased permeability of the paracellular tight junction as a consequence of myosin light chain ...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: He WQ,Wang J,Sheng JY,Zha JM,Graham WV,Turner JR

    更新日期:2020-02-03 00:00:00

  • A Tale of Two Proteolytic Machines: Matrix Metalloproteinases and the Ubiquitin-Proteasome System in Pulmonary Fibrosis.

    abstract::Pulmonary fibrosis is a chronic and progressive lung disease characterized by the activation of fibroblasts and the irreversible deposition of connective tissue matrices that leads to altered pulmonary architecture and physiology. Multiple factors have been implicated in the pathogenesis of lung fibrosis, including ge...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Roque W,Boni A,Martinez-Manzano J,Romero F

    更新日期:2020-05-29 00:00:00

  • Metagenomic Analysis of Upwelling-Affected Brazilian Coastal Seawater Reveals Sequence Domains of Type I PKS and Modular NRPS.

    abstract::Marine environments harbor a wide range of microorganisms from the three domains of life. These microorganisms have great potential to enable discovery of new enzymes and bioactive compounds for industrial use. However, only ~1% of microorganisms from the environment can currently be identified through cultured isolat...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Cuadrat RR,Cury JC,Dávila AM

    更新日期:2015-11-27 00:00:00

  • Mitochondria-Targeting Antioxidant Provides Cardioprotection through Regulation of Cytosolic and Mitochondrial Zn2+ Levels with Re-Distribution of Zn2+-Transporters in Aged Rat Cardiomyocytes.

    abstract::Aging is an important risk factor for cardiac dysfunction. Heart during aging exhibits a depressed mechanical activity, at least, through mitochondria-originated increases in ROS. Previously, we also have shown a close relationship between increased ROS and cellular intracellular free Zn2+ ([Zn2+]i) in cardiomyocytes ...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Olgar Y,Tuncay E,Turan B

    更新日期:2019-08-02 00:00:00

  • Comparative Proteomics Analyses of Pollination Response in Endangered Orchid Species Dendrobium Chrysanthum.

    abstract::Pollination is a crucial stage in plant reproductive process. The self-compatibility (SC) and self-incompatibility (SI) mechanisms determined the plant genetic diversity and species survival. D. chrysanthum is a highly valued ornamental and traditional herbal orchid in Asia but has been declared endangered. The sexual...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Wang W,Yu H,Li T,Li L,Zhang G,Liu Z,Huang T,Zhang Y

    更新日期:2017-11-23 00:00:00