In vitro effects of omega-3 fatty acids on neutrophil intracellular calcium homeostasis and receptor expression for FMLP and LTB4.


:Diets enriched in omega-3 fatty acids exert antiinflammatory properties by suppressing some neutrophil (PMN) functions. Changes in cytosolic Ca2+ concentration, [Ca2+]i, are important for PMN activation and are in part regulated by membrane Ca2+ ATPases. Since membrane proteins are influenced by their lipid environment, we investigated the in vitro effects of the omega-3 fatty acids eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) on the [Ca2+]i of PMNs in response to f-Met-Leu-Phe (FMLP), leukotriene B4 (LTB4), and ionomycin. The resting [Ca2+]i of PMNs (in high Ca2+ environment) was increased after pretreatment (37 degrees C, 2 h) with DHA, but not with EPA, or the other fatty acids, oleic acid (OA), or linolenic acid (LA). The stimulated [Ca2+]i by either FMLP or LTB4 was suppressed in a high Ca2+ environment after pretreatment with either EPA or DHA but not with OA or LA. The stimulated [Ca2+]i rise by ionomycin was augmented after pretreatment with DHA but not with EPA, OA, or LA. Pretreatment of PMNs with either EPA or DHA reduced the receptor expression for both FMLP and LTB4. Since omega-3 fatty acids inhibit the expression of receptors for two activators of PMNs, FMLP and LTB4, as well as the [Ca2+]i rise in response to those two stimuli, we propose that the antiinflammatory properties of EPA and DHA may be attributed, at least in part, to alteration in membrane activation of phagocytes.






Georgilis K,Klempner MS




Has Abstract


1988-10-01 00:00:00












  • Comparison of in vivo effects of intravenous infusion of N-formyl-methionyl-leucyl-phenylalanine and phorbol myristate acetate in rabbits.

    abstract::Comparison of the physiologic responses in rabbits to the intravenous infusion of two polymorphonuclear neutrophil (PMN) activators, N-formyl-methionyl-leucyl-phenylalanine (FMLP) and phorbol myristate acetate (PMA), has revealed marked differences in kinetics for activation between these agents. FMLP infusion was ass...


    pub_type: 杂志文章


    authors: Lafuze JE,Baker MD,Oakes AL,Baehner RL

    更新日期:1987-12-01 00:00:00

  • Icariin Attenuates High-cholesterol Diet Induced Atherosclerosis in Rats by Inhibition of Inflammatory Response and p38 MAPK Signaling Pathway.

    abstract::Icariin is a flavonoid isolated from the traditional Chinese herbal medicine Epimedium brevicornum Maxim and has been reported to be effective for the treatment of a variety of cardiovascular diseases. The aim of the present study was to investigate the effect and mechanism of icariin on atherosclerosis (AS) using a h...


    pub_type: 杂志文章


    authors: Hu Y,Sun B,Liu K,Yan M,Zhang Y,Miao C,Ren L

    更新日期:2016-02-01 00:00:00

  • Properties of lymphocyte chemotactic factor (lymphotactin) in thymus.

    abstract::Aqueous extracts of calf thymus from immunologically stimulated animals contain trypsin- and neuraminidase-labile macromolecules with molecular weights of less than 30,000 daltons which will specifically attract chemotactically lymphocytes. This activity we ascribe to "lymphotactin." ...


    pub_type: 杂志文章


    authors: Houck JC,Chang CM

    更新日期:1976-03-01 00:00:00

  • Decreased production of nitric oxide by human neutrophils during septic multiple organ dysfunction syndrome. Comparison with endotoxin and cytokine effects on normal cells.

    abstract::The objective of this study was to determine nitric oxide (NO) and superoxide anion release (O-2) by neutrophils (PMNs) in the septic multiple organ dysfunction syndrome (MODS) and to compare them with the response of normal cells to lipopolysaccharide (LPS) and cytokines. NO production was measured by the release of ...


    pub_type: 杂志文章


    authors: Carreras MC,Catz SD,Pargament GA,Del Bosco CG,Poderoso JJ

    更新日期:1994-04-01 00:00:00

  • Effect of guanosine nucleotides on the respiratory burst oxidase from human neutrophils.

    abstract::The respiratory burst is a series of metabolic events which result in the production of microbicidal oxidants by phagocytes. The enzyme responsible for the respiratory burst is a membrane-bound oxidase which catalyzes the production of O-2 by the NADPH-dependent reduction of oxygen. Activity of this oxidase is diminis...


    pub_type: 杂志文章


    authors: Kaufman MR,Babior BM

    更新日期:1983-09-01 00:00:00

  • Melanoma Differentiation-Associated Gene 5 Positively Modulates TNF-α-Induced CXCL10 Expression in Cultured HuH-7 and HLE Cells.

    abstract::The molecular mechanisms of innate immunity are closely associated with the development of non-alcoholic fatty liver disease (NAFLD). TNF-α is a key cytokine involved in the pathogenesis of metabolic inflammation like NAFLD. Melanoma differentiation-associated gene 5 (MDA5) is a member of the intracellular RNA helicas...


    pub_type: 杂志文章


    authors: Kawaguchi S,Sakuraba H,Haga T,Matsumiya T,Seya K,Endo T,Sawada N,Iino C,Kikuchi H,Hiraga H,Fukuda S,Imaizumi T

    更新日期:2019-12-01 00:00:00

  • Difference in the blood monocyte phenotype between uremic patients and healthy controls: its relation to monocyte differentiation into macrophages in the peritoneal cavity.

    abstract::The phenotypic alterations between blood monocytes from 11 patients with end-stage renal disease, who had been on peritoneal dialysis for less than one week, and blood monocytes from 10 healthy controls, were analyzed. In addition, peritoneal macrophages in the dialysate effluent were enclosed. Analysis of functional ...


    pub_type: 杂志文章


    authors: Brauner A,Lu Y,Halldén G,Hylander B,Lundahl J

    更新日期:1998-02-01 00:00:00

  • Protein kinase C inhibitor calphostin C prevents cytokine-induced angiogenesis in the rat.

    abstract::A rat sponge implant model was used to examine the role of protein kinase C (PKC) in angiogenesis. Neovascular response was determined by measurements of relative sponge blood flow by a 133Xe clearance technique and confirmed histologically. Morphometric analysis was used to quantitate the amount of fibrovascular grow...


    pub_type: 杂志文章


    authors: Hu DE,Fan TP

    更新日期:1995-02-01 00:00:00

  • The Effect of Pressure-Controlled Ventilation and Volume-Controlled Ventilation in Prone Position on Pulmonary Mechanics and Inflammatory Markers.

    abstract::The aim of this present study is to compare the effect of pressure-controlled ventilation and volume-controlled ventilation on pulmonary mechanics and inflammatory markers in prone position. The study included 41 patients undergoing to vertebrae surgery. The patients were randomized into two groups: Group 1 received v...


    pub_type: 杂志文章,随机对照试验


    authors: Şenay H,Sıvacı R,Kokulu S,Koca B,Bakı ED,Ela Y

    更新日期:2016-08-01 00:00:00

  • Protective Effects of Isoliquiritigenin on LPS-Induced Acute Lung Injury by Activating PPAR-γ.

    abstract::Isoliquiritigenin (ILG), a major ingredient of licorice, has been reported to have anti-oxidative and anti-inflammatory effects. The aim of this study was to investigate the protective effects of ILG on lung injury using an animal model of lipopolysaccharide (LPS)-induced acute lung injury (ALI). Male BALB/c mice were...


    pub_type: 杂志文章


    authors: Zhang W,Wang G,Zhou S

    更新日期:2018-08-01 00:00:00

  • Expression of the HML-1 Epitope on Human Monocytes is Independent of αE Integrin mRNA.

    abstract::Interferon gamma (IFNγ)-mediated activation of the myelomonocytic cell line HL-60 and peripheral blood monocytes will induce expression of an epitope for the monoclonal antibody which recognizes human αEβ7 integrin, HML-1. Here, we found that the anti-human αEβ7 monoclonal antibody Ber-ACT8 did not recognize IFNγ-stim...


    pub_type: 杂志文章


    authors: Miller LA,Li C,Hyde DM

    更新日期:2000-06-01 00:00:00

  • The Effects of Quercetin on Acute Lung Injury and Biomarkers of Inflammation and Oxidative Stress in the Rat Model of Sepsis.

    abstract::Experimental studies indicate that sepsis causes remote organ injury although the molecular mechanism has not been clearly defined. In this report, the role of oxidative damage, and inflammation on lung injury, following sepsis model by cecal ligation and puncture, and the effects of quercetin, antioxidant, and anti-i...


    pub_type: 杂志文章


    authors: Gerin F,Sener U,Erman H,Yilmaz A,Aydin B,Armutcu F,Gurel A

    更新日期:2016-04-01 00:00:00

  • Colchicine and methotrexate reduce leukocyte adherence and emigration in rat mesenteric venules.

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    pub_type: 杂志文章


    authors: Asako H,Kubes P,Baethge BA,Wolf RE,Granger DN

    更新日期:1992-02-01 00:00:00

  • Autologous versus allogeneic T cell-stimulated IL-6 production by dermal fibroblasts.

    abstract::T cells adhere to human dermal fibroblasts (HDF). This cellular interaction leads to a pronounced secretion of the proinflammatory cytokines IL-6 and IL-8 via a juxtacrine stimulation induced by HDF-associated IL-1. Upon stimulation, fibroblasts express various surface proteins such as MCH-I molecules, which may inter...


    pub_type: 杂志文章


    authors: Spörri B,Bickel M,Limat A,Waelti ER,Hunziker T,Wiesmann UN

    更新日期:1997-08-01 00:00:00

  • Modulation of endothelial cell permeability by lung carcinoma cells: a potential mechanism of malignant pleural effusion formation.

    abstract::This study examined the hypothesis that tumor cells metastatic to the pleura secrete a soluble factor(s) that directly increases endothelial cell permeability. Nitrocellulose filters were endothelialized with bovine pulmonary artery endothelial cells and exposed to conditioned media from either human lung adenocarcino...


    pub_type: 杂志文章


    authors: Payne DK,Fuseler JW,Owens MW

    更新日期:1994-08-01 00:00:00

  • Identification of Rab6a as a New Target of microRNA-155 Involved in Regulating Lipopolysaccharide-Induced TNF Secretion.

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    pub_type: 杂志文章


    authors: Yang Y,Yang L

    更新日期:2016-02-01 00:00:00

  • Do NSAIDs affect the progression of osteoarthritis?

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    pub_type: 杂志文章,评审


    authors: Ding C

    更新日期:2002-06-01 00:00:00

  • ApoM-S1P Modulates Ox-LDL-Induced Inflammation Through the PI3K/Akt Signaling Pathway in HUVECs.

    abstract::Studies have shown that apolipoprotein M (apoM), the main carrier of sphingosine-1-phosphate (S1P), is closely related to lipid metabolism and inflammation. While there are many studies on apoM and lipid metabolism, little is known about the role of apoM in inflammation. Atherosclerosis is a chronic inflammatory proce...


    pub_type: 杂志文章


    authors: Zheng Z,Zeng Y,Zhu X,Tan Y,Li Y,Li Q,Yi G

    更新日期:2019-04-01 00:00:00

  • Dichloromethane fraction of Laminaria japonica ethanolic extract inhibits lipopolysaccharide-induced nitric oxide synthase and cyclooxygenase-2 expression in RAW 264.7 cells via NF-κB pathway.

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    pub_type: 杂志文章


    authors: Lee JY,Lee MS,Choi JW,Shin TS,Woo HC,Kim HR

    更新日期:2012-10-01 00:00:00

  • Alpha-lipoic acid improves acetic acid-induced gastric ulcer healing in rats.

    abstract::To evaluate the role of ALA treatment on the healing of acetic acid-induced gastric ulcer, rats were given ALA (35 mg/kg/day) or saline for 3 days before the induction of ulcer and the treatment was continued twice daily for 2 days (early) or 10 days (late) until they were decapitated. Gastric ulcer index, microscopic...


    pub_type: 杂志文章


    authors: Karakoyun B,Yüksel M,Ercan F,Erzik C,Yeğen BC

    更新日期:2009-02-01 00:00:00

  • Genetic susceptibility to nosocomial pneumonia, acute respiratory distress syndrome and poor outcome in patients at risk of critical illness.

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    pub_type: 杂志文章


    authors: Salnikova LE,Smelaya TV,Vesnina IN,Golubev AM,Moroz VV

    更新日期:2014-04-01 00:00:00

  • Retinoic acid modulation of mucin mRNA in rat tracheal explants: response to actinomycin D, cycloheximide, signal transduction effectors and antisense oligodeoxynucleotide.

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    pub_type: 杂志文章


    authors: Bhattacharyya SN,Ashbaugh P,Kaufman B,Manna B

    更新日期:1994-12-01 00:00:00

  • Exogenous nitric oxide increases neutrophil adhesion to cultured human endothelial monolayers through a protein kinase G dependent mechanism.

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    pub_type: 杂志文章


    authors: Okayama N,Coe L,Itoh M,Alexander JS

    更新日期:1999-02-01 00:00:00

  • Rosiglitazone ameliorates diabetic nephropathy by reducing the expression of Chemerin and ChemR23 in the kidney of streptozotocin-induced diabetic rats.

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    pub_type: 杂志文章


    authors: Hu W,Yu Q,Zhang J,Liu D

    更新日期:2012-08-01 00:00:00

  • A rise in ionized calcium activates the neutrophil NADPH-oxidase but is not sufficient to directly translocate cytosolic p47phox or p67phox to b cytochrome containing membranes.

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    pub_type: 杂志文章


    authors: Movitz C,Sjölin C,Dahlgren C

    更新日期:1997-10-01 00:00:00

  • AETIQ: a novel synthetic compound with anti-inflammatory properties in activated microglia.

    abstract::Neuroinflammation is believed to be involved in the pathogenesis of neurodegenerative diseases. Our novel synthetic compound 2-acetyl-1-ethyl-7-hydroxy-6-methoxy-1, 2, 3, 4-tetrahydroisoquinoline (AETIQ) was tested for its anti-inflammatory properties in activated microglial BV-2 cells. AETIQ attenuated nitric oxide (...


    pub_type: 杂志文章


    authors: Son HJ,Shin N,Shin EJ,Chi DY,Seo JW,Lee CS,Hwang O

    更新日期:2014-06-01 00:00:00

  • IL-1β Upregulates IL-8 Production in Human Müller Cells Through Activation of the p38 MAPK and ERK1/2 Signaling Pathways.

    abstract::Diabetic retinopathy shares some similarity with chronic inflammation and Müller cells dysfunction may play an important role in its initiation and progression since these cells are thought to be a major source of inflammatory factors. The goal of this study was to examine the effect of cytokines on human retinal Müll...


    pub_type: 杂志文章


    authors: Liu X,Ye F,Xiong H,Hu D,Limb GA,Xie T,Peng L,Yang W,Sun Y,Zhou M,Song E,Zhang DY

    更新日期:2014-10-01 00:00:00

  • Influence of extracellular Ca2+ and Mg2+ on chemotactic factor-induced neutrophil aggregation.

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    pub_type: 杂志文章


    authors: O'Flaherty JT,Showell HJ,Ward PA

    更新日期:1977-12-01 00:00:00

  • Transient endotoxemia during burn wound revision causes leukocyte beta 2 integrin up-regulation and cytokine release.

    abstract::After severe burns, a wound revision is often done to remove devitalized tissue and minimize bacterial growth. After such revision, the patient may show signs of sepsis. In a group of burned patients we found a transient endotoxemia, and a subsequent leukocyte activation, monitored as increased expression of the beta ...


    pub_type: 杂志文章


    authors: Ljunghusen O,Berg S,Hed J,Lundahl J,Nettelblad H,Sjögren F,Stendahl O

    更新日期:1995-08-01 00:00:00

  • Growth Differentiation Factor-15 Is a Novel Biomarker Predicting Acute Exacerbation of Chronic Obstructive Pulmonary Disease.

    abstract::Exacerbations in chronic obstructive pulmonary disease (COPD) reduce quality of life and are associated with a more rapid deterioration of the disease. Growth differentiation factor-15 (GDF-15) is a novel candidate exacerbation biomarker. In this study, we aimed to assess GDF-15 as a biomarker of acute exacerbation of...


    pub_type: 杂志文章


    authors: Mutlu LC,Altintas N,Aydin M,Tulubas F,Oran M,Kucukyalin V,Kaplan G,Gurel A

    更新日期:2015-10-01 00:00:00