Downregulation of ATP1A1 promotes cancer development in renal cell carcinoma.


BACKGROUND:Aberrant expression of Na+/K+-ATPase α1 subunit (ATP1A1) is widely observed in multiple types of tumors, and its tissue-specific expression relates to cancer development. However, the functions and molecular mechanisms in renal cell carcinoma (RCC) are not fully understood. METHODS:We investigated the ATP1A1 expression changes and possible roles in RCC through a quantitative proteomic approach and an integrative biochemical assessment. We detected ATP1A1 in RCC with LC-MS/MS, and further validated its expression with immunohistochemical analyses of 80 pairs of the RCC tumor and non-tumor tissues samples. The association of ATP1A1 expression with RCC pathology was statistically analyzed. Cell proliferation, migration and apoptosis were measured by CCK-8, boyden chamber assay and flow cytometry, respectively. The production of reactive oxygen species (ROS) was labeled with a single staining using a commercial kit, and was further detected with flow cytometry. RESULTS:The ATP1A1 shows a significantly decreased expression in human RCC tissues than in the adjacent non-tumor tissues. The RCC patients with ATP1A1-positive expression exhibit longer overall survival time than the ATP1A1-negative patients. The exogenous overexpression of ATP1A1 inhibits RCC cell proliferation and cell migration by increasing the production of ROS. In addition, ATP1A1-mediated Raf/MEK/ERK signaling pathway is suppressed in RCC cells, indicating the possible occurrence of induced cell apoptosis. CONCLUSIONS:Our in vitro and in vivo data of ATP1A1 inhibitory roles in RCC progression suggest that ATP1A1 is a potential novel suppressor protein for renal cancer.


Clin Proteomics


Clinical proteomics


Zhang D,Zhang P,Yang P,He Y,Wang X,Yang Y,Zhu H,Xu N,Liang S




Has Abstract


2017-05-04 00:00:00












  • Biomarkers of blood cadmium and incidence of cardiovascular events in non-smokers: results from a population-based proteomics study.

    abstract:Background:Cadmium is a toxic metal with multiple adverse health effects, including risk of cardiovascular disease (CVD). The mechanistic link between cadmium and CVD is unclear. Our aim was to examine the associations between blood cadmium (B-Cd) and 88 potential protein biomarkers of CVD. Methods:B-Cd and 88 plasma ...

    journal_title:Clinical proteomics

    pub_type: 杂志文章


    authors: Borné Y,Fagerberg B,Sallsten G,Hedblad B,Persson M,Melander O,Nilsson J,Orho-Melander M,Barregard L,Engström G

    更新日期:2019-05-15 00:00:00

  • Advances in mass spectrometry-based clinical biomarker discovery.

    abstract::The greatest unmet needs in biomarker discovery are those discoveries that lead to the development of clinical diagnostic tests. These clinical diagnostic tests can provide early intervention when a patient would present otherwise healthy (e.g., cancer or cardiovascular disease) and aid clinical decision making with i...

    journal_title:Clinical proteomics

    pub_type: 杂志文章,评审


    authors: Crutchfield CA,Thomas SN,Sokoll LJ,Chan DW

    更新日期:2016-01-07 00:00:00

  • Discovery and validation of a colorectal cancer classifier in a new blood test with improved performance for high-risk subjects.

    abstract:BACKGROUND:The aim was to improve upon an existing blood-based colorectal cancer (CRC) test directed to high-risk symptomatic patients, by developing a new CRC classifier to be used with a new test embodiment. The new test uses a robust assay format-electrochemiluminescence immunoassays-to quantify protein concentratio...

    journal_title:Clinical proteomics

    pub_type: 杂志文章


    authors: Croner LJ,Dillon R,Kao A,Kairs SN,Benz R,Christensen IJ,Nielsen HJ,Blume JE,Wilcox B

    更新日期:2017-07-25 00:00:00

  • Quantification of angiotensin II-regulated proteins in urine of patients with polycystic and other chronic kidney diseases by selected reaction monitoring.

    abstract:BACKGROUND:Angiotensin-II (Ang II) mediates progression of autosomal-dominant polycystic kidney disease (ADPKD) and other chronic kidney diseases (CKD). However, markers of kidney Ang II activity are lacking. We previously defined 83 Ang II-regulated proteins in vitro, which reflected kidney Ang II activity in vivo. M...

    journal_title:Clinical proteomics

    pub_type: 杂志文章


    authors: Konvalinka A,Batruch I,Tokar T,Dimitromanolakis A,Reid S,Song X,Pei Y,Drabovich AP,Diamandis EP,Jurisica I,Scholey JW

    更新日期:2016-08-05 00:00:00

  • Down-regulation of kallikrein-related peptidase 5 (KLK5) expression in breast cancer patients: a biomarker for the differential diagnosis of breast lesions.

    abstract:BACKGROUND:Kallikrein-related peptidase 5 (KLK5) is a secreted trypsin-like protease of the KLK family, encoded by the KLK5 gene. KLK5 has been found to cleave various extracellular matrix components, as well as to activate several other KLK proteases, triggering the stimulation of tissue microenvironment proteolytic c...

    journal_title:Clinical proteomics

    pub_type: 杂志文章


    authors: Avgeris M,Papachristopoulou G,Polychronis A,Scorilas A

    更新日期:2011-05-31 00:00:00

  • Comprehensive proteome analysis of nasal lavage samples after controlled exposure to welding nanoparticles shows an induced acute phase and a nuclear receptor, LXR/RXR, activation that influence the status of the extracellular matrix.

    abstract:Background:Epidemiological studies have shown that many welders experience respiratory symptoms. During the welding process a large number of airborne nanosized particles are generated, which might be inhaled and deposited in the respiratory tract. Knowledge of the underlying mechanisms behind observed symptoms is stil...

    journal_title:Clinical proteomics

    pub_type: 杂志文章


    authors: Ali N,Ljunggren S,Karlsson HM,Wierzbicka A,Pagels J,Isaxon C,Gudmundsson A,Rissler J,Nielsen J,Lindh CH,Kåredal M

    更新日期:2018-05-11 00:00:00

  • The plasma peptides of breast versus ovarian cancer.

    abstract:Background:There is a need to demonstrate a proof of principle that proteomics has the capacity to analyze plasma from breast cancer versus other diseases and controls in a multisite clinical trial design. The peptides or proteins that show a high observation frequency, and/or precursor intensity, specific to breast ca...

    journal_title:Clinical proteomics

    pub_type: 杂志文章


    authors: Dufresne J,Bowden P,Thavarajah T,Florentinus-Mefailoski A,Chen ZZ,Tucholska M,Norzin T,Ho MT,Phan M,Mohamed N,Ravandi A,Stanton E,Slutsky AS,Dos Santos CC,Romaschin A,Marshall JC,Addison C,Malone S,Heyland D,Schelte

    更新日期:2019-12-23 00:00:00

  • The proteins cleaved by endogenous tryptic proteases in normal EDTA plasma by C18 collection of peptides for liquid chromatography micro electrospray ionization and tandem mass spectrometry.

    abstract::The tryptic peptides from ice cold versus room temperature plasma were identified by C18 liquid chromatography and micro electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS). Samples collected on ice showed low levels of endogenous tryptic peptides compared to the same samples incubated at room temperature....

    journal_title:Clinical proteomics

    pub_type: 杂志文章


    authors: Dufresne J,Florentinus-Mefailoski A,Ajambo J,Ferwa A,Bowden P,Marshall J

    更新日期:2017-12-02 00:00:00

  • Elucidating the endogenous synovial fluid proteome and peptidome of inflammatory arthritis using label-free mass spectrometry.

    abstract:Background:Inflammatory arthritis (IA) is an immunological disorder in which loss of immune tolerance to endogenous self-antigens perpetuates synovitis and eventual destruction of the underlying cartilage and bone. Pathological changes in the joint are expected to be represented by synovial fluid (SF) proteins and pept...

    journal_title:Clinical proteomics

    pub_type: 杂志文章


    authors: Mahendran SM,Keystone EC,Krawetz RJ,Liang K,Diamandis EP,Chandran V

    更新日期:2019-05-30 00:00:00

  • Mass spectrometry-based analysis of glycoproteins and its clinical applications in cancer biomarker discovery.

    abstract::Glycosylation is one of the most important posttranslational modifications of proteins and plays essential roles in various biological processes. Aberration in the glycan moieties of glycoproteins is associated with many diseases. It is especially critical to develop the rapid and sensitive methods for analysis of abe...

    journal_title:Clinical proteomics

    pub_type: 杂志文章


    authors: Liu H,Zhang N,Wan D,Cui M,Liu Z,Liu S

    更新日期:2014-04-10 00:00:00

  • Proteomic analysis of purified protein derivative of Mycobacterium tuberculosis.

    abstract:BACKGROUND:Purified protein derivative (PPD) has been used for more than half a century as an antigen for the diagnosis of tuberculosis infection based on delayed type hypersensitivity. Although designated as "purified," in reality, the composition of PPD is highly complex and remains ill-defined. In this report, high ...

    journal_title:Clinical proteomics

    pub_type: 杂志文章


    authors: Prasad TS,Verma R,Kumar S,Nirujogi RS,Sathe GJ,Madugundu AK,Sharma J,Puttamallesh VN,Ganjiwale A,Myneedu VP,Chatterjee A,Pandey A,Harsha H,Narayana J

    更新日期:2013-07-19 00:00:00

  • Identification of Cytauxzoon felis antigens via protein microarray and assessment of expression library immunization against cytauxzoonosis.

    abstract:Background:Cytauxzoonosis is a disease of felids in North America caused by the tick-transmitted apicomplexan parasite Cytauxzoon felis. Cytauxzoonosis is particularly virulent for domestic cats, but no vaccine currently exists. The parasite cannot be cultivated in vitro, presenting a significant limitation for vaccine...

    journal_title:Clinical proteomics

    pub_type: 杂志文章


    authors: Schreeg ME,Marr HS,Tarigo JL,Sherrill MK,Outi HK,Scholl EH,Bird DM,Vigil A,Hung C,Nakajima R,Liang L,Trieu A,Doolan DL,Thomas JE,Levy MG,Reichard MV,Felgner PL,Cohn LA,Birkenheuer AJ

    更新日期:2018-12-29 00:00:00

  • Proteomic analysis of human placental syncytiotrophoblast microvesicles in preeclampsia.

    abstract:BACKGROUND:Placental syncytiotrophoblast microvesicles (STBM) are shed into the maternal circulation during normal pregnancy. STBM circulate in significantly increased amounts in preeclampsia (PE) and are considered to be among contributors to the exaggerated proinflammatory, procoagulant state of PE. However, protein ...

    journal_title:Clinical proteomics

    pub_type: 杂志文章


    authors: Baig S,Kothandaraman N,Manikandan J,Rong L,Ee KH,Hill J,Lai CW,Tan WY,Yeoh F,Kale A,Su LL,Biswas A,Vasoo S,Choolani M

    更新日期:2014-11-19 00:00:00

  • Quantitative phosphoproteomic analysis reveals reciprocal activation of receptor tyrosine kinases between cancer epithelial cells and stromal fibroblasts.

    abstract:Background:Cancer-associated fibroblasts (CAFs) are one of the most important components of tumor stroma and play a key role in modulating tumor growth. However, a mechanistic understanding of how CAFs communicate with tumor cells to promote their proliferation and invasion is far from complete. A major reason for this...

    journal_title:Clinical proteomics

    pub_type: 杂志文章


    authors: Wu X,Zahari MS,Renuse S,Sahasrabuddhe NA,Chaerkady R,Kim MS,Fackler MJ,Stampfer M,Gabrielson E,Sukumar S,Pandey A

    更新日期:2018-06-15 00:00:00

  • CD44 glycoprotein in cancer: a molecular conundrum hampering clinical applications.

    abstract::CD44 is a heavily glycosylated membrane receptor playing a key role in cell adhesion, signal transduction and cytoskeleton remodelling. It is also one of the most studied glycoproteins in cancer, frequently explored for stem cell identification, and associated with chemoresistance and metastasis. However, CD44 is a ge...

    journal_title:Clinical proteomics

    pub_type: 杂志文章


    authors: Azevedo R,Gaiteiro C,Peixoto A,Relvas-Santos M,Lima L,Santos LL,Ferreira JA

    更新日期:2018-06-27 00:00:00

  • Global proteome profiling of human livers upon ischemia/reperfusion treatment.

    abstract::Hepatic ischemia/reperfusion (I/R) injury represents a major risk factor for liver transplantation and is related to graft dysfunction and acute/chronic rejection. However, a significant part of these processes remain poorly characterized. To reveal differences in the proteome during liver I/R injury, we collected hum...

    journal_title:Clinical proteomics

    pub_type: 杂志文章


    authors: Cai H,Qi S,Yan Q,Ling J,Du J,Chen L

    更新日期:2021-01-06 00:00:00

  • Potential urine proteomics biomarkers for primary nephrotic syndrome.

    abstract:BACKGROUND:Nephrotic syndrome (NS) is a nonspecific kidney disorder, commonly caused by minimal change disease (MCD), focal segmental glomerulosclerosis (FSGS), and membranous nephropathy (MN). Here we analyzed urinary protein profiles, aiming to discover disease-specific biomarkers of these three common diseases in NS...

    journal_title:Clinical proteomics

    pub_type: 杂志文章


    authors: Choi YW,Kim YG,Song MY,Moon JY,Jeong KH,Lee TW,Ihm CG,Park KS,Lee SH

    更新日期:2017-05-16 00:00:00

  • Proteomic profiling of lung adenocarcinoma indicates heightened DNA repair, antioxidant mechanisms and identifies LASP1 as a potential negative predictor of survival.

    abstract:BACKGROUND:Lung cancer is the leading cause of cancer mortality in the United States. Non-small cell lung cancer accounts for 85% of all lung cancers for which adenocarcinoma is the most common histological type. Management of lung cancer is hindered by high false-positive rates due to difficulty resolving between beni...

    journal_title:Clinical proteomics

    pub_type: 杂志文章


    authors: Fahrmann JF,Grapov D,Phinney BS,Stroble C,DeFelice BC,Rom W,Gandara DR,Zhang Y,Fiehn O,Pass H,Miyamoto S

    更新日期:2016-10-27 00:00:00

  • Proteomic analysis of plasma exosomes to differentiate malignant from benign pulmonary nodules.

    abstract:Background:It is difficult to distinguish benign pulmonary nodules (PNs) from malignant PNs by conventional examination. Therefore, novel biomarkers that can identify the nature of PNs are needed. Exosomes have recently been identified as an attractive alternative approach since tumor-specific molecules can be found in...

    journal_title:Clinical proteomics

    pub_type: 杂志文章


    authors: Kuang M,Tao X,Peng Y,Zhang W,Pan Y,Cheng L,Yuan C,Zhao Y,Mao H,Zhuge L,Zhou Z,Chen H,Sun Y

    更新日期:2019-02-02 00:00:00

  • The impact of ultraviolet- and infrared-based laser microdissection technology on phosphoprotein detection in the laser microdissection-reverse phase protein array workflow.

    abstract::Reversible protein phosphorylation represents a key mechanism by which signals are transduced in eukaryotic cells. Dysregulated phosphorylation is also a hallmark of carcinogenesis and represents key drug targets in the precision medicine space. Thus, methods that preserve phosphoprotein integrity in the context of cl...

    journal_title:Clinical proteomics

    pub_type: 杂志文章


    authors: Hunt AL,Pierobon M,Baldelli E,Oliver J,Mitchell D,Gist G,Bateman NW,Larry Maxwell G,Petricoin EF,Conrads TP

    更新日期:2020-03-09 00:00:00

  • Cardiovascular proteomics in the era of big data: experimental and computational advances.

    abstract::Proteomics plays an increasingly important role in our quest to understand cardiovascular biology. Fueled by analytical and computational advances in the past decade, proteomics applications can now go beyond merely inventorying protein species, and address sophisticated questions on cardiac physiology. The advent of ...

    journal_title:Clinical proteomics

    pub_type: 杂志文章,评审


    authors: Lam MP,Lau E,Ng DC,Wang D,Ping P

    更新日期:2016-12-05 00:00:00

  • Phosphotyrosine profiling of curcumin-induced signaling.

    abstract:BACKGROUND:Curcumin, derived from the rhizome Curcuma longa, is a natural anti-cancer agent and has been shown to inhibit proliferation and survival of tumor cells. Although the anti-cancer effects of curcumin are well established, detailed understanding of the signaling pathways altered by curcumin is still lacking. I...

    journal_title:Clinical proteomics

    pub_type: 杂志文章


    authors: Sathe G,Pinto SM,Syed N,Nanjappa V,Solanki HS,Renuse S,Chavan S,Khan AA,Patil AH,Nirujogi RS,Nair B,Mathur PP,Prasad TSK,Gowda H,Chatterjee A

    更新日期:2016-06-15 00:00:00

  • Phosphotyrosine profiling of human cerebrospinal fluid.

    abstract:Background:Cerebrospinal fluid (CSF) is an important source of potential biomarkers that affect the brain. Biomarkers for neurodegenerative disorders are needed to assist in diagnosis, monitoring disease progression and evaluating efficacy of therapies. Recent studies have demonstrated the involvement of tyrosine kinas...

    journal_title:Clinical proteomics

    pub_type: 杂志文章


    authors: Sathe G,Na CH,Renuse S,Madugundu A,Albert M,Moghekar A,Pandey A

    更新日期:2018-09-12 00:00:00

  • Proteomic characterization of high-density lipoprotein particles in patients with non-alcoholic fatty liver disease.

    abstract:Background:Metabolic diseases such as obesity and diabetes are associated with changes in high-density lipoprotein (HDL) particles, including changes in particle size and protein composition, often resulting in abnormal function. Recent studies suggested that patients with non-alcoholic fatty liver disease (NAFLD), inc...

    journal_title:Clinical proteomics

    pub_type: 杂志文章


    authors: Rao PK,Merath K,Drigalenko E,Jadhav AYL,Komorowski RA,Goldblatt MI,Rohatgi A,Sarzynski MA,Gawrieh S,Olivier M

    更新日期:2018-03-06 00:00:00

  • Challenges and opportunities of bovine milk analysis by mass spectrometry.

    abstract::Bovine milk and its products (e.g. cheese, yoghurt) are an important part of human diet with beneficial effects for all ages. Although analyses of different milk components (e.g. proteins, lipids) pose huge challenges, the use of mass spectrometric (MS)-based techniques is steadily improving our understanding of the c...

    journal_title:Clinical proteomics

    pub_type: 杂志文章,评审


    authors: Verma A,Ambatipudi K

    更新日期:2016-04-19 00:00:00

  • Serum proteomic-based analysis identifying autoantibodies against PRDX2 and PRDX3 as potential diagnostic biomarkers in nasopharyngeal carcinoma.

    abstract:BACKGROUND:Nasopharyngeal carcinoma (NPC) is a major head and neck cancer with high occurrence in Southeast Asia and southern China. We aimed to identify autoantibodies that may contribute to early detection of NPC. METHODS:We used serological proteome analysis to identify candidate autoantibodies against tumor-associ...

    journal_title:Clinical proteomics

    pub_type: 杂志文章


    authors: Lin LH,Xu YW,Huang LS,Hong CQ,Zhai TT,Liao LD,Lin WJ,Xu LY,Zhang K,Li EM,Peng YH

    更新日期:2017-02-01 00:00:00

  • Proteomics in India: the clinical aspect.

    abstract::Proteomics has emerged as a highly promising bioanalytical technique in various aspects of applied biological research. In Indian academia, proteomics research has grown remarkably over the last decade. It is being extensively used for both basic as well as translation research in the areas of infectious and immune di...

    journal_title:Clinical proteomics

    pub_type: 杂志文章,评审


    authors: Mukherjee S,Bandyopadhyay A

    更新日期:2016-11-05 00:00:00

  • N-GlycositeAtlas: a database resource for mass spectrometry-based human N-linked glycoprotein and glycosylation site mapping.

    abstract:Background:N-linked glycoprotein is a highly interesting class of proteins for clinical and biological research. The large-scale characterization of N-linked glycoproteins accomplished by mass spectrometry-based glycoproteomics has provided valuable insights into the interdependence of glycoprotein structure and protei...

    journal_title:Clinical proteomics

    pub_type: 杂志文章


    authors: Sun S,Hu Y,Ao M,Shah P,Chen J,Yang W,Jia X,Tian Y,Thomas S,Zhang H

    更新日期:2019-09-07 00:00:00

  • Mass spectrometry-based analyses showing the effects of secretor and blood group status on salivary N-glycosylation.

    abstract:BACKGROUND:The carbohydrate portions of salivary glycoproteins play important roles, including mediating bacterial and leukocyte adhesion. Salivary glycosylation is complex. Many of its glycoproteins present ABO and Lewis blood group determinants. An individual's genetic complement and secretor status govern the expres...

    journal_title:Clinical proteomics

    pub_type: 杂志文章


    authors: Albertolle ME,Hassis ME,Ng CJ,Cuison S,Williams K,Prakobphol A,Dykstra AB,Hall SC,Niles RK,Ewa Witkowska H,Fisher SJ

    更新日期:2015-12-30 00:00:00

  • Functional proteomics can define prognosis and predict pathologic complete response in patients with breast cancer.

    abstract:PURPOSE:To determine whether functional proteomics improves breast cancer classification and prognostication and can predict pathological complete response (pCR) in patients receiving neoadjuvant taxane and anthracycline-taxane-based systemic therapy (NST). METHODS:Reverse phase protein array (RPPA) using 146 antibodi...

    journal_title:Clinical proteomics

    pub_type: 杂志文章


    authors: Gonzalez-Angulo AM,Hennessy BT,Meric-Bernstam F,Sahin A,Liu W,Ju Z,Carey MS,Myhre S,Speers C,Deng L,Broaddus R,Lluch A,Aparicio S,Brown P,Pusztai L,Symmans WF,Alsner J,Overgaard J,Borresen-Dale AL,Hortobagyi GN,Co

    更新日期:2011-07-08 00:00:00