Cannabidiol administration reduces sublesional cancellous bone loss in rats with severe spinal cord injury.


:Patients with spinal cord injury (SCI) undergo severe loss of bone mineral below the level of lesion, and data on available treatment options after SCI is scarce. The aim of this work was to investigate the therapeutic effect of cannabidiol (CBD), a non-psychoactive cannabis, on sublesional bone loss in a rat model of SCI. The adult male rats were exposed to surgical transection of the cord and treated with CBD for consecutive 14 days. It was found that CBD treatment elevated the serum levels of osteocalcin, reduced the serum levels of collagen type I cross-linked C-telopeptide, and enhanced bone mineral density of tibiae and femurs. Treatment of SCI rats with CBD enhanced bone volume, trabecular thickness, and trabecular number, and reduced trabecular separation in proximal tibiae, and increased ultimate compressive load, stiffness, and energy to max force of femoral diaphysis. Treatment of SCI rats with CBD upregulated mRNA expression of alkaline phosphatase and osteoprotegerin and downregulated mRNA expression of receptor activator of NF-κB ligand and tartrate-resistant acid phosphatase in femurs. Furthermore, treatment of SCI rats with CBD enhanced mRNA expression of wnt3a, Lrp5 and ctnnb1 in femurs. In conclusion, CBD administration attenuated SCI-induced sublesional cancellous bone loss.


Eur J Pharmacol


Li D,Lin Z,Meng Q,Wang K,Wu J,Yan H




Has Abstract


2017-08-15 00:00:00












  • Effect of cyclophosphamide on gene expression of cytochromes p450 and beta-actin in the HL-60 cell line.

    abstract::Many studies have demonstrated that cyclophosphamide (CPA) can affect hepatic cytochrome p450 (CYP) isoenzyme activity in animals. We have investigated the effect of CPA on gene expression of various CYP enzymes as well as beta-actin in the human acute promyelocytic leukemia cell line (HL-60S) and its multidrug-resist...

    journal_title:European journal of pharmacology

    pub_type: 杂志文章


    authors: Xie HJ,Lundgren S,Broberg U,Finnström N,Rane A,Hassan M

    更新日期:2002-08-09 00:00:00

  • Tiapamil reduces the calcium inward current of isolated smooth muscle cells. Dependence on holding potential and pulse frequency.

    abstract::The calcium currents (ICa) of isolated smooth muscle cells (urinary bladder of the guinea-pig) were analyzed at 35 degrees C and 3.6 mM [Ca]0. The whole cells were voltage-clamped with a single patch electrode which was filled with 150 mM CsCl in order to block potassium outward currents. Tiapamil reduced ICa at conce...

    journal_title:European journal of pharmacology

    pub_type: 杂志文章


    authors: Klöckner U,Isenberg G

    更新日期:1986-08-15 00:00:00

  • Alterations of beta-receptor-adenylyl cyclase coupling by long-term ATP depletion in cultured rat cardiomyocytes.

    abstract::In cultured rat heart muscle cells, reversible long-term ATP depletion induces a decrease in beta-adrenoceptor density and a fall in isoproterenol- as well as forskolin-stimulated cAMP formation. However, isoproterenol-stimulated adenylyl cyclase activity in membrane preparations is not reduced after ATP depletion. Th...

    journal_title:European journal of pharmacology

    pub_type: 杂志文章


    authors: Wagenknecht B,Beuttler C

    更新日期:1991-11-13 00:00:00

  • Comparative effects of K+ channel blockade on the vasorelaxant activity of cromakalim, pinacidil and nicorandil.

    abstract::Three agents with K+ channel blocking activity, procaine, 4-aminopyridine (4-AP) and tetraethylammonium (TEA), were tested for inhibition of vasorelaxation and 86Rb+ efflux induced by cromakalim (BRL 34915), pinacidil and nicorandil in rabbit isolated mesenteric artery. The potency order for inhibition of vasorelaxati...

    journal_title:European journal of pharmacology

    pub_type: 杂志文章


    authors: Wilson C,Coldwell MC,Howlett DR,Cooper SM,Hamilton TC

    更新日期:1988-08-02 00:00:00

  • Impaired cyclic AMP generation in outer medullary tubules of gentamicin-treated rats.

    abstract::We have examined the effects of chronic gentamicin treatment on arginine8-vasopressin (AVP)-dependent cyclic AMP (cAMP) metabolism in rat medullary collecting tubules (oMCT) and medullary thick ascending limbs of Henle's loop (mTALH). Gentamicin attenuated AVP-stimulated cAMP accumulation to a greater extent in the mT...

    journal_title:European journal of pharmacology

    pub_type: 杂志文章


    authors: Kidwell D,Subramaniam S,Ott C,Jackson B

    更新日期:1990-12-04 00:00:00

  • Bufotalin from Venenum Bufonis inhibits growth of multidrug resistant HepG2 cells through G2/M cell cycle arrest and apoptosis.

    abstract::Venenum Bufonis, a traditional Chinese medicine, is widely used in the treatment of liver cancer in modern Chinese medical practices. In our search for anti-hepatoma constituents in Venenum Bufonis, bufotalin, bufalin, telocinobufagin and cinobufagin were obtained. Bufotalin was the most potent active compound among t...

    journal_title:European journal of pharmacology

    pub_type: 杂志文章


    authors: Zhang DM,Liu JS,Tang MK,Yiu A,Cao HH,Jiang L,Chan JY,Tian HY,Fung KP,Ye WC

    更新日期:2012-10-05 00:00:00

  • N-methyl-D-aspartate and alpha 2-adrenergic mechanisms are involved in the depressant action of flupirtine on spinal reflexes in rats.

    abstract::In urethane-chloralose anesthetised rats the muscle relaxant activity of flupirtine was investigated on the monosynaptic Hoffmann reflex recorded from plantar foot muscles and on the polysynaptic flexor reflex recorded from tibialis muscle. Intraperitoneal (i.p.; 2.5-25 mumol/kg) and intrathecal (i.t.; 33-330 nmol) ad...

    journal_title:European journal of pharmacology

    pub_type: 杂志文章


    authors: Schwarz M,Schmitt T,Pergande G,Block F

    更新日期:1995-04-04 00:00:00

  • Activation of KCNQ5 channels stably expressed in HEK293 cells by BMS-204352.

    abstract::The novel anti-ischemic compound, BMS-204352 ((3S)-(+)-(5-chloro-2-methoxyphenyl)-1,3-dihydro-3-fluoro-6-(trifluoromethyl)-2H-indol-2-one)), strongly activates the voltage-gated K+ channel KCNQ5 in a concentration-dependent manner with an EC50 of 2.4 microM. At 10 microM, BMS-204352 increased the steady state current ...

    journal_title:European journal of pharmacology

    pub_type: 杂志文章


    authors: Dupuis DS,Schrøder RL,Jespersen T,Christensen JK,Christophersen P,Jensen BS,Olesen SP

    更新日期:2002-02-22 00:00:00

  • Constitutive androstane receptor activation by 2,4,6-triphenyldioxane-1,3 suppresses the expression of the gluconeogenic genes.

    abstract::The constitutive androstane receptor (CAR, NR1I3) has a central role in detoxification processes, regulating the expression of a set of genes involved in metabolism. The dual role of NR1I3 as both a xenosensor and as a regulator of endogenous energy metabolism has recently been accepted. Here, we investigated the mech...

    journal_title:European journal of pharmacology

    pub_type: 杂志文章


    authors: Kachaylo EM,Yarushkin AA,Pustylnyak VO

    更新日期:2012-03-15 00:00:00

  • Contractile actions of imidazoline alpha-adrenoceptor agonists and effects of noncompetitive alpha1-adrenoceptor antagonists in human vas deferens.

    abstract::The contractile actions of imidazoline alpha-adrenoceptor agonists were investigated in human vas deferens longitudinal and circular muscle. The effects of phenoxybenzamine were studied in comparison to dibenamine and SZL-49 (4-amino-6,7-dimethoxy-2-quinazolinyl-4-(2-bicyclo[2,2,2]octa-2,5-dienylcarbonyl-2-piperazine)...

    journal_title:European journal of pharmacology

    pub_type: 杂志文章


    authors: Amobi N,Guillebaud J,Kaisary A,Lloyd-Davies RW,Turner E,Smith IC

    更新日期:2003-02-21 00:00:00

  • Broad therapeutic treatment window of [Nle(4), D-Phe(7)]alpha-melanocyte-stimulating hormone for long-lasting protection against ischemic stroke, in Mongolian gerbils.

    abstract::Melanocortin peptides have been shown to produce neuroprotection in experimental ischemic stroke. The aim of the present investigation was to identify the therapeutic treatment window of melanocortins, and to determine whether these neuropeptides chronically protect against damage consequent to brain ischemia. A 10-mi...

    journal_title:European journal of pharmacology

    pub_type: 杂志文章


    authors: Giuliani D,Leone S,Mioni C,Bazzani C,Zaffe D,Botticelli AR,Altavilla D,Galantucci M,Minutoli L,Bitto A,Squadrito F,Guarini S

    更新日期:2006-05-24 00:00:00

  • Melanin potentiates gentamicin-induced inhibition of collagen biosynthesis in human skin fibroblasts.

    abstract::One of the recognized side effects of gentamicin is ototoxicity. The mechanism underlying the organ specificity of this side effect of gentamicin has not been fully established. In view of the fact that a number of pharmacologic agents are known to form complexes with melanin and melanin is an abundant constituent of ...

    journal_title:European journal of pharmacology

    pub_type: 杂志文章


    authors: Wrześniok D,Buszman E,Karna E,Nawrat P,Palka J

    更新日期:2002-06-20 00:00:00

  • Selective cytotoxicity of the antibacterial peptide ABP-dHC-Cecropin A and its analog towards leukemia cells.

    abstract::Some cationic antibacterial peptides, with typical amphiphilic α-helical conformations in a membrane-mimicking environment, exhibit anticancer properties as a result of a similar mechanism of action towards both bacteria and cancer cells. We previously reported the cDNA sequence of the antimicrobial peptide ABP-dHC-Ce...

    journal_title:European journal of pharmacology

    pub_type: 杂志文章


    authors: Sang M,Zhang J,Zhuge Q

    更新日期:2017-05-15 00:00:00

  • In vitro and ex vivo characterization of (-)-TZ659 as a ligand for imaging the vesicular acetylcholine transporter.

    abstract::The loss of cholinergic neurons and synapses relates to the severity of dementia in several neurodegenerative pathologies; and the vesicular acetylcholine transporter (VAChT) provides a reliable biomarker of cholinergic function. We recently characterized and (11)C-labeled a new VAChT inhibitor, (-)-TZ659. Here we rep...

    journal_title:European journal of pharmacology

    pub_type: 杂志文章


    authors: Liu H,Jin H,Li J,Zhang X,Kaneshige K,Parsons SM,Perlmutter JS,Tu Z

    更新日期:2015-04-05 00:00:00

  • Pharmacological profile of YM358, a novel nonpeptide angiotensin AT1 receptor antagonist.

    abstract::The pharmacological profile of YM358, 2,7-diethyl-5-[[2'-(1 H-tetrazol-5-yl)biphenyl-4-yl]methyl]-5H-pyrazolo[1,5-b][1,2,4]tri azole potassium salt monohydrate, a novel non-peptide angiotensin AT1 receptor antagonist, was studied in vitro and in vivo. YM358 competed with [125I][Sar1, Ile8]angiotensin II for angiotensi...

    journal_title:European journal of pharmacology

    pub_type: 杂志文章


    authors: Shibasaki M,Fujimori A,Takanashi M,Kusayama T,Tokioka T,Satoh Y,Okazaki T,Uchida W,Inagaki O,Yanagisawa I

    更新日期:1997-09-24 00:00:00

  • Baroreceptor-induced decrease in muscle blood flow upon propranolol administration.

    abstract::The acute effects of propranolol, 1 mg/kg i.v., were studied in chloralosed, vagotomized cats. The vascularly isolated but innervated calf muscles were perfused from another animal. In one group of experiments, the carotid baroreceptors were exposed to ambient arterial pressure. Here, propranolol caused a fall in hear...

    journal_title:European journal of pharmacology

    pub_type: 杂志文章


    authors: Lisander B,Nilsson H

    更新日期:1978-08-01 00:00:00

  • Effect of N-methyl-D-aspartate on motor activity and in vivo adenosine striatal outflow in the rat.

    abstract::It has been previously found that the systemic administration of low doses of N-methyl-D-aspartate (NMDA) in mice induces motor depression. The effects of the systemic administration of different doses of NMDA (10, 30 and 60 mg/kg s.c.) on the motor activity and on the in vivo extracellular levels of adenosine in the ...

    journal_title:European journal of pharmacology

    pub_type: 杂志文章


    authors: Melani A,Corsi C,Giménez-Llort L,Martínez E,Ogren SO,Pedata F,Ferré S

    更新日期:1999-11-26 00:00:00

  • Differential effects of selective and non-selective inhibition of nitric oxide synthase on the expression and activity of cyclooxygenase-2 during gastric ulcer healing.

    abstract::Nitric oxide synthases (NOS) and cyclooxygenase-2 (COX-2) are important enzymes involved in ulcer healing but interactions between them have not been clearly defined. The aim of this study was to investigate the effects of selective or non-selective inhibition of NOS on the expression and activity of COX-2 during heal...

    journal_title:European journal of pharmacology

    pub_type: 杂志文章


    authors: Guo JS,Cho CH,Wang JY,Koo MW

    更新日期:2006-05-01 00:00:00

  • Kappa-opiates and urination: pharmacological evidence for an endogenous role of the kappa-opiate receptor in fluid and electrolyte balance.

    abstract::In prehydrated rats, the administration of kappa-opiate agonists such as bremazocine, ethylketocyclazocine or compound Upjohn-50,488 produced a dose-dependent increase in urine output and decreased the concentration of Na+ and K+ in the urine as compared to that of saline-treated rats. The diuretic effect of bremazoci...

    journal_title:European journal of pharmacology

    pub_type: 杂志文章


    authors: Huidobro-Toro JP,Parada S

    更新日期:1984-12-15 00:00:00

  • Rutin ameliorates diabetic neuropathy by lowering plasma glucose and decreasing oxidative stress via Nrf2 signaling pathway in rats.

    abstract::Rutin exhibits antidiabetic, antioxidant and anti-inflammatory properties, which makes rutin an attractive candidate for diabetic complications. The present study was designed to investigate the potential effect of rutin on diabetic neuropathy. After induction of diabetic neuropathy, rutin (5mg/kg, 25mg/kg and 50mg/kg...

    journal_title:European journal of pharmacology

    pub_type: 杂志文章


    authors: Tian R,Yang W,Xue Q,Gao L,Huo J,Ren D,Chen X

    更新日期:2016-01-15 00:00:00

  • Endogenous nitric oxide counteracts antigen-induced bronchoconstriction.

    abstract::In anesthetized, artificially ventilated guinea pigs immunized against ovalbumin, challenge with aerosolized ovalbumin (0.1 mg) elicited a substantial and sustained increase of insufflation pressure. The inhibitor of endogenous nitric oxide (NO) synthesis, L-NAME (N omega-nitro-L-arginine methylester, 30 mg kg-1 i.v.)...

    journal_title:European journal of pharmacology

    pub_type: 杂志文章


    authors: Persson MG,Friberg SG,Hedqvist P,Gustafsson LE

    更新日期:1993-11-16 00:00:00

  • A monoclonal antibody to calcitonin gene-related peptide abolishes capsaicin-induced gastroprotection.

    abstract::Calcitonin gene-related peptide (CGRP) released from vasodilator nerves is implicated in the gastroprotective action of capsaicin. This experimental paradigm was used to prove the effectiveness of a monoclonal anti-CGRP antibody. The experiments were performed in anaesthetized rats in which intragastric capsaicin (0.5...

    journal_title:European journal of pharmacology

    pub_type: 杂志文章


    authors: Peskar BM,Wong HC,Walsh JH,Holzer P

    更新日期:1993-11-30 00:00:00

  • Behavioural topography in the striatum: differential effects of quinpirole and D-amphetamine microinjections.

    abstract::Behavioural evidence has accumulated that supports the hypothesis that specific territories of the striatum contribute differentially to the control of motor behaviours. The present experiments compare the behavioural effects of microinjections of amphetamine (20 microg/0.5 microl) with those elicited by the D2-class ...

    journal_title:European journal of pharmacology

    pub_type: 杂志文章


    authors: Canales JJ,Iversen SD

    更新日期:1998-12-04 00:00:00

  • DHA supplementation prevent the progression of NASH via GPR120 signaling.

    abstract::Nonalcoholic steatohepatitis (NASH) is one of the most common liver diseases involving chronic accumulation of fat and inflammation, often leading to advanced fibrosis, cirrhosis and carcinoma. However, the pathological mechanism for this is unknown. GPR120/FFAR4 has been recognized as a functional fatty acid receptor...

    journal_title:European journal of pharmacology

    pub_type: 杂志文章


    authors: Nakamoto K,Shimada K,Harada S,Morimoto Y,Hirasawa A,Tokuyama S

    更新日期:2018-02-05 00:00:00

  • Alterations in the myocardial beta-adrenoceptor system of streptozotocin-diabetic rats.

    abstract::Previous investigations in our laboratory revealed subsensitivity of right ventricular tissue, isolated from one month STZ-diabetic rats, to the inotropic effects of isoproterenol. The present study was concerned with the characterization of this subsensitivity phenomenon. Observations of supersensitivity to methoxami...

    journal_title:European journal of pharmacology

    pub_type: 杂志文章


    authors: Ramanadham S,Tenner TE Jr

    更新日期:1987-04-29 00:00:00

  • Expression of purinergic P2Y receptor subtypes by INS-1 insulinoma beta-cells: a molecular and binding characterization.

    abstract::Purinergic P2Y-receptor agonists amplify glucose-induced insulin secretion from pancreatic beta-cells, thus offering new opportunities for the treatment of type 2 diabetes. However, little is known about which subtypes of purinergic P2Y receptors are expressed in these cells. The INS-1 beta-cell line is used as a mode...

    journal_title:European journal of pharmacology

    pub_type: 杂志文章


    authors: Lugo-Garcia L,Filhol R,Lajoix AD,Gross R,Petit P,Vignon J

    更新日期:2007-07-30 00:00:00

  • Loss of amitriptyline analgesia in alpha 2A-adrenoceptor deficient mice.

    abstract::Tricyclic antidepressants have analgesic and sedative effects in addition to their antidepressive properties. We tested the acute analgesic and locomotor inhibitory effects of the tricyclic antidepressant amitriptyline and the alpha(2)-adrenoceptor agonist clonidine in wild-type control and in alpha(2A)-adrenoceptor k...

    journal_title:European journal of pharmacology

    pub_type: 杂志文章


    authors: Ozdoğan UK,Lähdesmäki J,Mansikka H,Scheinin M

    更新日期:2004-02-06 00:00:00

  • Vascular beta-adrenoceptor blocking activity of endotoxin and pertussis toxin from Bordetella pertussis in rats.

    abstract::Isolated and purified leucocytosis promoting factor (LPF), alternatively described as pertussis toxin, reduced the hypotension after beta 2-adrenoceptor stimulation with salbutamol as well as the negative chronotropic activity induced by the muscarinic receptor stimulant arecoline 4 days after its injection into rats....

    journal_title:European journal of pharmacology

    pub_type: 杂志文章


    authors: De Wildt DJ,De Jong Y,Nijkamp FP,Kreeftenberg JG

    更新日期:1986-08-15 00:00:00

  • Cholinergic regulation of thyrotropin secretion in male rats.

    abstract::The cold-stimulated thyrotropin (TSH) secretion in male rats was suppressed by muscarinic agonists, i.e. Oxa-22, McN-A-343 (an M1 agonist), oxotremorine (an M2 agonist) and methacholine (a quaternary compound). The inhibitory effect of Oxa-22 was antagonized by atropine, butylscopolamine and glycopyrrolate as well as ...

    journal_title:European journal of pharmacology

    pub_type: 杂志文章


    authors: Männistö PT,Suomela A,Rauhala P,Tuominen RK,Suolinna T

    更新日期:1988-11-22 00:00:00

  • PLGA nanoparticles loaded with beta-lactoglobulin-derived peptides modulate mucosal immunity and may facilitate cow's milk allergy prevention.

    abstract::Beta-lactoglobulin (BLG)-derived peptides may facilitate oral tolerance to whey and prevent cow's milk allergy (CMA). Loading of BLG-peptides in poly(lactic-co-glycolic acid) (PLGA) nanoparticles (Pep-NP) may improve this. Here we studied the uptake of NP and the capacity of NP and Pep-NP to activate bone marrow dendr...

    journal_title:European journal of pharmacology

    pub_type: 杂志文章


    authors: Kostadinova AI,Middelburg J,Ciulla M,Garssen J,Hennink WE,Knippels LMJ,van Nostrum CF,Willemsen LEM

    更新日期:2018-01-05 00:00:00