Single-nucleotide polymorphism typing analysis for molecular subtyping of Salmonella Tennessee isolates associated with the 2007 nationwide peanut butter outbreak in the United States.


BACKGROUND:In 2007, a nationwide Salmonella Tennessee outbreak occurred via contaminated peanut butter. Here, we developed a single-nucleotide polymorphism (SNP)-typing method for S. Tennessee to determine the clonal subtypes of S. Tennessee that were associated with the peanut butter outbreak. METHODS AND RESULTS:One seventy-six S. Tennessee isolates from various sources, including humans, animals, food, and the environment, were analyzed by using the SNP technique. Eighty-four representative SNP markers were selected by comparing the sequences of three representative S. Tennessee strains with different multi-locus sequence typing and variable number tandem repeats from our collection. The set of eighty-four SNP markers showed 100% typeability for the 176 strains, with the nucleotide diversity ranging from 0.011 to 0.107 (mean = 0.049 ± 0.018, median = 0.044) for each marker. Among the four clades and nine subtypes generated by the SNP typing, subtype 1, which comprised 142 S. Tennessee strains, was the most predominant. The dominance of single-strain clones in subtype 1 revealed that S. Tennessee is highly clonal regardless of outbreak-association, source, or period of isolation, suggesting the presence of an S. Tennessee strain prototype. Notably, a minimum 18 SNP set was able to determine clonal S. Tennessee strains with similar discrimination power, potentially allowing more rapid and economic strain genotyping for both outbreaks and sporadic cases. CONCLUSIONS:The SNP-typing method described here might aid the investigation of the epidemiology and microevolution of pathogenic bacteria by discriminating between outbreak-related and sporadic clinical cases. In addition, this approach enables us to understand the population structure of the bacterial subtypes involved in the outbreak.


Gut Pathog


Gut pathogens


Dong HJ,Cho S,Boxrud D,Rankin S,Downe F,Lovchik J,Gibson J,Erdman M,Saeed AM




Has Abstract


2017-05-01 00:00:00










  • Blood profile holds clues to role of infection in a premonitory state for idiopathic parkinsonism and of gastrointestinal infection in established disease.

    abstract::The two-stage neuroinflammatory process, containment and progression, proposed to underlie neurodegeneration may predicate on systemic inflammation arising from the gastrointestinal tract. Helicobacter infection has been described as one switch in the pathogenic-circuitry of idiopathic parkinsonism (IP): eradication m...

    journal_title:Gut pathogens

    pub_type: 杂志文章


    authors: Charlett A,Dobbs RJ,Dobbs SM,Weller C,Ibrahim MA,Dew T,Sherwood R,Oxlade NL,Plant JM,Bowthorpe J,Lawson AJ,Curry A,Peterson DW,Bjarnason IT

    更新日期:2009-11-26 00:00:00

  • Antimicrobial resistances do not affect colonization parameters of intestinal E. coli in a small piglet group.

    abstract:BACKGROUND:Although antimicrobial resistance and persistence of resistant bacteria in humans and animals are major health concerns worldwide, the impact of antimicrobial resistance on bacterial intestinal colonization in healthy domestic animals has only been rarely studied. We carried out a retrospective analysis of t...

    journal_title:Gut pathogens

    pub_type: 杂志文章


    authors: Schierack P,Kadlec K,Guenther S,Filter M,Schwarz S,Ewers C,Wieler LH

    更新日期:2009-10-08 00:00:00

  • Genetic variants of Helicobacter pylori type IV secretion system components CagL and CagI and their association with clinical outcomes.

    abstract:BACKGROUND:Helicobacter pylori infection is associated with risk for chronic gastritis (CG), gastric ulcer (GU), duodenal ulcer (DU), and gastric cancer (GC). The H. pylori Cag type IV secretion system (TFSS) translocates the virulence factor cytotoxin-associated gene A protein into host cells and plays an important ro...

    journal_title:Gut pathogens

    pub_type: 杂志文章


    authors: Ogawa H,Iwamoto A,Tanahashi T,Okada R,Yamamoto K,Nishiumi S,Yoshida M,Azuma T

    更新日期:2017-04-21 00:00:00

  • Draft genomes of four enterotoxigenic Escherichia coli (ETEC) clinical isolates from China and Bangladesh.

    abstract:BACKGROUND:Enterotoxigenic Escherichia coli (ETEC) is an important pathogen that causes childhood and travelers' diarrhea. Here, we present the draft genomes of four ETEC isolates recovered from stool specimens of patients with diarrhea in Beijing, China and Dhaka, Bangladesh, respectively. RESULTS:We obtained the dra...

    journal_title:Gut pathogens

    pub_type: 杂志文章


    authors: Liu F,Yang X,Wang Z,Nicklasson M,Qadri F,Yi Y,Zhu Y,Lv N,Li J,Zhang R,Guo H,Zhu B,Sjöling Å,Hu Y

    更新日期:2015-04-08 00:00:00

  • Genome sequencing, annotation and comparative genomic analysis of Shigella dysenteriae strain SD1D.

    abstract:BACKGROUND:Shigellosis is an acute form of gastroenteritis caused by the bacteria belonging to the genus Shigella. It is the most common cause of morbidity and mortality in children. Shigella belongs to the family Enterobactericeae, which is a Gram-negative and rod shaped bacterium. In the present study, we report the ...

    journal_title:Gut pathogens

    pub_type: 杂志文章


    authors: Kaur G,Sathyabama S,Arora A,Verma S,Mubin N,Agrewala JN,Mayilraj S

    更新日期:2014-07-11 00:00:00

  • ESBL-plasmid carriage in E. coli enhances in vitro bacterial competition fitness and serum resistance in some strains of pandemic sequence types without overall fitness cost.

    abstract:Background:Extended spectrum beta lactamase (ESBL)-producing extraintestinal pathogenic Escherichia coli infections are of global interest because of their clinical and economic impact. The ESBL resistance genes disseminate through plasmids, and are found in successful global lineages such as ST131 and ST648. The carri...

    journal_title:Gut pathogens

    pub_type: 杂志文章


    authors: Ranjan A,Scholz J,Semmler T,Wieler LH,Ewers C,Müller S,Pickard DJ,Schierack P,Tedin K,Ahmed N,Schaufler K,Guenther S

    更新日期:2018-06-15 00:00:00

  • Role of TLR1, TLR2 and TLR6 in the modulation of intestinal inflammation and Candida albicans elimination.

    abstract:BACKGROUND:Toll-like receptors (TLRs) are the major pattern recognition receptors that mediate sensing of a wide range of microorganisms. TLR2 forms heterodimers with either TLR1 or TLR6, broadening its ligand diversity against pathogens. TLR1, TLR2 and TLR6 have been implicated in the recognition of Candida albicans, ...

    journal_title:Gut pathogens

    pub_type: 杂志文章


    authors: Choteau L,Vancraeyneste H,Le Roy D,Dubuquoy L,Romani L,Jouault T,Poulain D,Sendid B,Calandra T,Roger T,Jawhara S

    更新日期:2017-02-15 00:00:00

  • Comparison of clinical and immunological findings in gnotobiotic piglets infected with Escherichia coli O104:H4 outbreak strain and EHEC O157:H7.

    abstract:BACKGROUND:Shiga toxin (Stx) producing Escherichia coli (E. coli) (STEC) is the most frequent cause of diarrhoea-positive haemolytic uraemic syndrome (D + HUS) in humans. In 2011, a huge outbreak with an STEC O104:H4 strain in Germany highlighted the limited possibilities for causative treatment of this syndrome. The r...

    journal_title:Gut pathogens

    pub_type: 杂志文章


    authors: Wöchtl B,Gunzer F,Gerner W,Gasse H,Koch M,Bagó Z,Ganter M,Weissenböck H,Dinhopl N,Coldewey SM,von Altrock A,Waldmann KH,Saalmüller A,Zimmermann K,Steinmann J,Kehrmann J,Klein-Hitpass L,Blom J,Ehricht R,Engelmann I,

    更新日期:2017-05-25 00:00:00

  • No evidence of the Shiga toxin-producing E. coli O104:H4 outbreak strain or enteroaggregative E. coli (EAEC) found in cattle faeces in northern Germany, the hotspot of the 2011 HUS outbreak area.

    abstract:BACKGROUND:Ruminants, in particular bovines, are the primary reservoir of Shiga toxin-producing E. coli (STEC), but whole genome analyses of the current German ESBL-producing O104:H4 outbreak strain of sequence type (ST) 678 showed this strain to be highly similar to enteroaggregative E. coli (EAEC). Strains of the EAE...

    journal_title:Gut pathogens

    pub_type: 杂志文章


    authors: Wieler LH,Semmler T,Eichhorn I,Antao EM,Kinnemann B,Geue L,Karch H,Guenther S,Bethe A

    更新日期:2011-11-03 00:00:00

  • Insights of biosurfactant producing Serratia marcescens strain W2.3 isolated from diseased tilapia fish: a draft genome analysis.

    abstract:BACKGROUND:Serratia marcescens is an opportunistic bacterial pathogen with broad range of host ranging from vertebrates, invertebrates and plants. S. marcescens strain W2.3 was isolated from a diseased tilapia fish and it was suspected to be the causal agent for the fish disease as virulence genes were found within its...

    journal_title:Gut pathogens

    pub_type: 杂志文章


    authors: Chan XY,Chang CY,Hong KW,Tee KK,Yin WF,Chan KG

    更新日期:2013-10-22 00:00:00

  • Transcriptome changes and polymyxin resistance of acid-adapted Escherichia coli O157:H7 ATCC 43889.

    abstract:BACKGROUND:Acid treatment is commonly used for controlling or killing pathogenic microorganisms on medical devices and environments; however, inadequate acid treatment may cause acid tolerance response (ATR) and offer cross-protection against environmental stresses, including antimicrobials. This study aimed to charact...

    journal_title:Gut pathogens

    pub_type: 杂志文章


    authors: Hwang D,Kim SM,Kim HJ

    更新日期:2020-12-01 00:00:00

  • Draft genome sequences of two Bifidobacterium sp. from the honey bee (Apis mellifera).

    abstract:BACKGROUND:Widely considered probiotic organisms, Bifidobacteria are common inhabitants of the alimentary tract of animals including insects. Bifidobacteria identified from the honey bee are found in larval guts and throughout the alimentary tract, but attain their greatest abundance in the adult hind gut. To further u...

    journal_title:Gut pathogens

    pub_type: 杂志文章


    authors: Anderson KE,Johansson A,Sheehan TH,Mott BM,Corby-Harris V,Johnstone L,Sprissler R,Fitz W

    更新日期:2013-12-18 00:00:00

  • Infectious bursal disease virus inoculation infection modifies Campylobacter jejuni-host interaction in broilers.

    abstract:Background:Campylobacter jejuni is considered as a chicken commensal. The gut microbiota and the immune status of the host may affect its colonization. Infectious bursal disease virus (IBDV) is an immunosuppressive virus of chickens, which allows secondary pathogens to invade or exacerbates their pathogenesis. To inves...

    journal_title:Gut pathogens

    pub_type: 杂志文章


    authors: Li L,Pielsticker C,Han Z,Kubasová T,Rychlik I,Kaspers B,Rautenschlein S

    更新日期:2018-03-30 00:00:00

  • Comparison of phenotypic methods for the detection of carbapenem non-susceptible Enterobacteriaceae.

    abstract:BACKGROUND:Multidrug resistance and, in particular, carbapenem resistance is spreading worldwide at an alarming rate, comprehending a variety of bacterial species and causing both nosocomial and community acquired outbursts. Early and efficient detection of infected patients or colonized carriers are mandatory steps in...

    journal_title:Gut pathogens

    pub_type: 杂志文章


    authors: Bartolini A,Frasson I,Cavallaro A,Richter SN,Palù G

    更新日期:2014-05-19 00:00:00

  • Serologic host response to Helicobacter pylori and Campylobacter jejuni in socially housed Rhesus macaques (Macaca mulatta).

    abstract:UNLABELLED: BACKGROUND:Helicobacter pylori are successful colonizers of the human gastric mucosa. Colonization increases the risk of peptic ulcer disease and adenocarcinoma. However, potential benefits of H. pylori colonization include protection against early-onset asthma and against gastrointestinal infections. Camp...

    journal_title:Gut pathogens

    pub_type: 杂志文章


    authors: Kienesberger S,Perez-Perez GI,Rivera-Correa JL,Tosado-Acevedo R,Li H,Dubois A,Gonzalez-Martinez JA,Dominguez-Bello MG,Blaser MJ

    更新日期:2012-08-24 00:00:00

  • Multiple etiologies of infectious diarrhea and concurrent infections in a pediatric outpatient-based screening study in Odisha, India.

    abstract:BACKGROUND:There are multiple etiologies responsible for infectious gastroenteritis causing acute diarrhea which are often under diagnosed. Also acute diarrhea is one of the major causes of morbidity and mortality among children less than 5 years of age. METHODS:In our study, fecal samples (n = 130) were collected fro...

    journal_title:Gut pathogens

    pub_type: 杂志文章


    authors: Shrivastava AK,Kumar S,Mohakud NK,Suar M,Sahu PS

    更新日期:2017-04-11 00:00:00

  • Stool cultures show a lack of impact in the management of acute gastroenteritis for hospitalized patients in the Bronx, New York.

    abstract:Background:Acute gastroenteritis (AGE) is diagnosed with a presentation of > 1 episode of vomiting and > 3 episodes of diarrhea in a 24-h period. Treatment is supportive, however, in severe cases antibacterial treatment may be indicated. Stool cultures can detect the responsible pathogenic bacteria and can guide antibi...

    journal_title:Gut pathogens

    pub_type: 杂志文章


    authors: Fraij O,Castro N,de Leon Castro LA,Brandt LJ

    更新日期:2020-06-22 00:00:00

  • The impact of Rhodiola rosea on the gut microbial community of Drosophila melanogaster.

    abstract:Background:The root extract of Rhodiola rosea has historically been used in Europe and Asia as an adaptogen, and similar to ginseng and Shisandra, shown to display numerous health benefits in humans, such as decreasing fatigue and anxiety while improving mood, memory, and stamina. A similar extract in the Rhodiola fami...

    journal_title:Gut pathogens

    pub_type: 杂志文章


    authors: Labachyan KE,Kiani D,Sevrioukov EA,Schriner SE,Jafari M

    更新日期:2018-03-20 00:00:00

  • Phase-variable restriction/modification systems are required for Helicobacter pylori colonization.

    abstract:BACKGROUND:One mechanism utilized by bacterial pathogens for host adaptation and immune evasion is the generation of phenotypic diversity by the phasevarion that results from the differential expression of a suite of genes regulated by the activity of a phase-variable methyltransferase within a restriction modification...

    journal_title:Gut pathogens

    pub_type: 杂志文章


    authors: Gauntlett JC,Nilsson HO,Fulurija A,Marshall BJ,Benghezal M

    更新日期:2014-09-05 00:00:00

  • Existence of a novel qepA variant in quinolone resistant Escherichia coli from aquatic habitats of Bangladesh.

    abstract::Of 19 environmental Escherichia coli (n = 12) and Klebsiella pneumoniae (n = 7) tested for quinolone resistance-related genes qnrA, qnrB, qnrC, qnrS and qepA, four each of E. coli and K. pneumoniae possessed qnrS, and another E. coli isolate possessed a new variant of qepA. This is the first detection of qepA in envir...

    journal_title:Gut pathogens

    pub_type: 杂志文章


    authors: Rahman Z,Islam A,Rashid MU,Johura FT,Monira S,Watanabe H,Ahmed N,Camilli A,Alam M

    更新日期:2017-10-23 00:00:00

  • Genotyping of Salmonella Typhi using 8-loci multi locus VNTR analysis.

    abstract:BACKGROUND:Typhoid fever has caused severe epidemics in many Asian and African countries. The early detection of outbreaks and their sources may promote the prevention and control of typhoid fever, for which effective and timely molecular subtyping techniques are required. Pulsed field gel electrophoresis (PFGE) is rou...

    journal_title:Gut pathogens

    pub_type: 杂志文章


    authors: Wang H,Diao B,Cui Z,Yan M,Kan B

    更新日期:2016-04-04 00:00:00

  • Exposure to bile influences biofilm formation by Listeria monocytogenes.

    abstract::In the present study we demonstrate that the initial attachment of Listeria monocytogenes cells to plastic surfaces was significantly increased by growth in the presence of bile. Improved biofilm formation was confirmed by crystal violet staining, microscopy and bioluminescence detection of a luciferase-tagged strain....

    journal_title:Gut pathogens

    pub_type: 杂志文章


    authors: Begley M,Kerr C,Hill C

    更新日期:2009-05-28 00:00:00

  • Preliminary feasibility for recruiting and retaining black and white females to provide fecal samples for longitudinal research.

    abstract::As the associations between the gut microbiota and numerous health outcomes become more evident, it is important to conduct longitudinal microbiome research to advance the field beyond the identification of associations. It is also necessary to include individuals who have historically been underrepresented in biomedi...

    journal_title:Gut pathogens

    pub_type: 杂志文章


    authors: Carson TL,Little RB,Townsend S

    更新日期:2019-08-26 00:00:00

  • Bacteriophage-encoded shiga toxin gene in atypical bacterial host.

    abstract:BACKGROUND:Contamination from fecal bacteria in recreational waters is a major health concern since bacteria capable of causing human disease can be found in animal feces. The Dog Beach area of Ocean Beach in San Diego, California is a beach prone to closures due to high levels of fecal indicator bacteria (FIB). A pote...

    journal_title:Gut pathogens

    pub_type: 杂志文章


    authors: Casas V,Sobrepeña G,Rodriguez-Mueller B,Ahtye J,Maloy SR

    更新日期:2011-07-07 00:00:00

  • Systematic review and meta-analysis: the relationship between the Helicobacter pylori dupA gene and clinical outcomes.

    abstract:BACKGROUND:In 2005, the first disease-specific Helicobacter pylori virulence factor that induced duodenal ulcer and had a suppressive action on gastric cancer has been identified, and was named duodenal ulcer promoting gene (dupA). However, the importance of the dupA gene on clinical outcomes is conflicting in subseque...

    journal_title:Gut pathogens

    pub_type: 杂志文章


    authors: Shiota S,Matsunari O,Watada M,Hanada K,Yamaoka Y

    更新日期:2010-10-31 00:00:00

  • Synergistic effects of Bifidobacterium thermophilum RBL67 and selected prebiotics on inhibition of Salmonella colonization in the swine proximal colon PolyFermS model.

    abstract:BACKGROUND:Probiotics and prebiotics are promising strategies to counteract Salmonella prevalence in swine. In the present study, we investigated the effects of prebiotics (fructo- (FOS), galacto- (GOS) and mannan- (MOS) oligosaccharides) and the bacteriocinogenic Bifidobacterium thermophilum RBL67 (RBL67) on Salmonell...

    journal_title:Gut pathogens

    pub_type: 杂志文章


    authors: Tanner SA,Chassard C,Zihler Berner A,Lacroix C

    更新日期:2014-10-24 00:00:00

  • Comparative genomic analysis reveals genetic features related to the virulence of Bacillus cereus FORC_013.

    abstract:BACKGROUND:Bacillus cereus is well known as a gastrointestinal pathogen that causes food-borne illness. In the present study, we sequenced the complete genome of B. cereus FORC_013 isolated from fried eel in South Korea. To extend our understanding of the genomic characteristics of FORC_013, we conducted a comparative ...

    journal_title:Gut pathogens



    authors: Koo HJ,Ahn S,Chung HY,Kim S,Kim K,Ryu S,Lee JH,Choi SH,Kim H

    更新日期:2017-05-15 00:00:00

  • The neurotoxic effects of ampicillin-associated gut bacterial imbalances compared to those of orally administered propionic acid in the etiology of persistent autistic features in rat pups: effects of various dietary regimens.

    abstract:HYPOTHESIS:A healthy gut with normal intestinal microflora is completely disrupted by oral antibiotics. The byproducts of harmful gut bacteria can interfere with brain development and may contribute to autism. Strategies to improve the gut microflora profile through dietary modification may help to alleviate gut disord...

    journal_title:Gut pathogens

    pub_type: 杂志文章


    authors: El-Ansary A,Bhat RS,Al-Daihan S,Al Dbass AM

    更新日期:2015-03-22 00:00:00

  • Intestinal microbiota, probiotics and mental health: from Metchnikoff to modern advances: part III - convergence toward clinical trials.

    abstract::Rapid scientific and technological advances have allowed for a more detailed understanding of the relevance of intestinal microbiota, and the entire body-wide microbiome, to human health and well-being. Rodent studies have provided suggestive evidence that probiotics (e.g. lactobacillus and bifidobacteria) can influen...

    journal_title:Gut pathogens

    pub_type: 杂志文章


    authors: Bested AC,Logan AC,Selhub EM

    更新日期:2013-03-16 00:00:00

  • Leukocyte-subset counts in idiopathic parkinsonism provide clues to a pathogenic pathway involving small intestinal bacterial overgrowth. A surveillance study.

    abstract:UNLABELLED: BACKGROUND:Following Helicobacter pylori eradication in idiopathic parkinsonism (IP), hypokinesia improved but flexor-rigidity increased. Small intestinal bacterial-overgrowth (SIBO) is a candidate driver of the rigidity: hydrogen-breath-test-positivity is common in IP and case histories suggest that Helic...

    journal_title:Gut pathogens

    pub_type: 杂志文章


    authors: Dobbs RJ,Charlett A,Dobbs SM,Weller C,A Ibrahim MA,Iguodala O,Smee C,Plant JM,Lawson AJ,Taylor D,Bjarnason I

    更新日期:2012-10-19 00:00:00